LOCUS       NM_002950               2284 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens ribophorin I (RPN1), mRNA.
ACCESSION   NM_002950
VERSION     NM_002950.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Lampson,B.L., Ramiotarez,A.S., Baro,M., He,L., Hegde,M., Koduri,V.,
            Pfaff,J.L., Hanna,R.E., Kowal,J., Shirole,N.H., He,Y., Doench,J.G.,
            Contessa,J.N., Locher,K.P. and Kaelin,W.G. Jr.
  TITLE     Positive selection CRISPR screens reveal a druggable pocket in an
            oligosaccharyltransferase required for inflammatory signaling to
            NF-kappaB
  JOURNAL   Cell 187 (9), 2209-2223 (2024)
   PUBMED   38670073
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Gemmer,M., Chaillet,M.L., van Loenhout,J., Cuevas Arenas,R.,
            Vismpas,D., Grollers-Mulderij,M., Koh,F.A., Albanese,P.,
            Scheltema,R.A., Howes,S.C., Kotecha,A., Fedry,J. and Forster,F.
  TITLE     Visualization of translation and protein biogenesis at the ER
            membrane
  JOURNAL   Nature 614 (7946), 160-167 (2023)
   PUBMED   36697828
REFERENCE   3  (bases 1 to 2284)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   4  (bases 1 to 2284)
  AUTHORS   Boughton,A.J., Zhang,D., Singh,R.K. and Fushman,D.
  TITLE     Polyubiquitin and ubiquitin-like signals share common recognition
            sites on proteasomal subunit Rpn1
  JOURNAL   J Biol Chem 296, 100450 (2021)
   PUBMED   33617881
  REMARK    GeneRIF: Polyubiquitin and ubiquitin-like signals share common
            recognition sites on proteasomal subunit Rpn1.
REFERENCE   5  (bases 1 to 2284)
  AUTHORS   Buljan,M., Ciuffa,R., van Drogen,A., Vichalkovski,A., Mehnert,M.,
            Rosenberger,G., Lee,S., Varjosalo,M., Pernas,L.E., Spegg,V.,
            Snijder,B., Aebersold,R. and Gstaiger,M.
  TITLE     Kinase Interaction Network Expands Functional and Disease Roles of
            Human Kinases
  JOURNAL   Mol Cell 79 (3), 504-520 (2020)
   PUBMED   32707033
REFERENCE   6  (bases 1 to 2284)
  AUTHORS   Elsasser,S., Gali,R.R., Schwickart,M., Larsen,C.N., Leggett,D.S.,
            Muller,B., Feng,M.T., Tubing,F., Dittmar,G.A. and Finley,D.
  TITLE     Proteasome subunit Rpn1 binds ubiquitin-like protein domains
  JOURNAL   Nat Cell Biol 4 (9), 725-730 (2002)
   PUBMED   12198498
REFERENCE   7  (bases 1 to 2284)
  AUTHORS   Fu,J. and Kreibich,G.
  TITLE     Retention of subunits of the oligosaccharyltransferase complex in
            the endoplasmic reticulum
  JOURNAL   J Biol Chem 275 (6), 3984-3990 (2000)
   PUBMED   10660554
REFERENCE   8  (bases 1 to 2284)
  AUTHORS   Kumar,V., Heinemann,F.S. and Ozols,J.
  TITLE     Interleukin-2 induces N-glycosylation in T-cells: characterization
            of human lymphocyte oligosaccharyltransferase
  JOURNAL   Biochem Biophys Res Commun 247 (2), 524-529 (1998)
   PUBMED   9642163
REFERENCE   9  (bases 1 to 2284)
  AUTHORS   Pekarsky,Y., Rynditch,A., Wieser,R., Fonatsch,C. and Gardiner,K.
  TITLE     Activation of a novel gene in 3q21 and identification of intergenic
            fusion transcripts with ecotropic viral insertion site I in
            leukemia
  JOURNAL   Cancer Res 57 (18), 3914-3919 (1997)
   PUBMED   9307271
REFERENCE   10 (bases 1 to 2284)
  AUTHORS   Crimaudo,C., Hortsch,M., Gausepohl,H. and Meyer,D.I.
  TITLE     Human ribophorins I and II: the primary structure and membrane
            topology of two highly conserved rough endoplasmic
            reticulum-specific glycoproteins
  JOURNAL   EMBO J 6 (1), 75-82 (1987)
   PUBMED   3034581
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC010839.1 and AC079945.21.
            
            On Nov 22, 2018 this sequence version replaced NM_002950.3.
            
            Summary: This gene encodes a type I integral membrane protein found
            only in the rough endoplasmic reticulum. The encoded protein is
            part of an N-oligosaccharyl transferase complex that links high
            mannose oligosaccharides to asparagine residues found in the
            Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This
            protein forms part of the regulatory subunit of the 26S proteasome
            and may mediate binding of ubiquitin-like domains to this
            proteasome. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189658.160778.1, BC010839.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968968
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000296255.8/ ENSP00000296255.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-440               BC010839.1         20-459
            441-441             AC079945.21        111574-111574
            442-1223            BC010839.1         461-1242
            1224-1224           AC079945.21        123640-123640
            1225-2038           BC010839.1         1244-2057
            2039-2040           AC079945.21        129369-129370
            2041-2142           BC010839.1         2058-2159
            2143-2143           AC079945.21        129473-129473
            2144-2237           BC010839.1         2161-2254
            2238-2283           BC010839.1         2258-2303
            2284-2284           AC079945.21        129614-129614
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q21.3"
     gene            1..2284
                     /gene="RPN1"
                     /gene_synonym="OST1; RBPH1"
                     /note="ribophorin I"
                     /db_xref="GeneID:6184"
                     /db_xref="HGNC:HGNC:10381"
                     /db_xref="MIM:180470"
     exon            1..279
                     /gene="RPN1"
                     /gene_synonym="OST1; RBPH1"
                     /inference="alignment:Splign:2.1.0"
     CDS             19..1842
                     /gene="RPN1"
                     /gene_synonym="OST1; RBPH1"
                     /EC_number="2.4.99.18"
                     /note="oligosaccharyltransferase 1 homolog;
                     dolichyl-diphosphooligosaccharide-protein
                     glycosyltransferase 67 kDa subunit;
                     dolichyl-diphosphooligosaccharide--protein
                     glycosyltransferase subunit 1; RPN-I; ribophorin-1;
                     oligosaccharyltransferase complex subunit (non-catalytic)"
                     /codon_start=1
                     /product="dolichyl-diphosphooligosaccharide--protein
                     glycosyltransferase subunit 1 precursor"
                     /protein_id="NP_002941.1"
                     /db_xref="CCDS:CCDS3051.1"
                     /db_xref="GeneID:6184"
                     /db_xref="HGNC:HGNC:10381"
                     /db_xref="MIM:180470"
                     /translation="MEAPAAGLFLLLLLGTWAPAPGSASSEAPPLINEDVKRTVDLSS
                     HLAKVTAEVVLAHLGGGSTSRATSFLLALEPELEARLAHLGVQVKGEDEEENNLEVRE
                     TKIKGKSGRFFTVKLPVALDPGAKISVIVETVYTHVLHPYPTQITQSEKQFVVFEGNH
                     YFYSPYPTKTQTMRVKLASRNVESYTKLGNPTRSEDLLDYGPFRDVPAYSQDTFKVHY
                     ENNSPFLTITSMTRVIEVSHWGNIAVEENVDLKHTGAVLKGPFSRYDYQRQPDSGISS
                     IRSFKTILPAAAQDVYYRDEIGNVSTSHLLILDDSVEMEIRPRFPLFGGWKTHYIVGY
                     NLPSYEYLYNLGDQYALKMRFVDHVFDEQVIDSLTVKIILPEGAKNIEIDSPYEISRA
                     PDELHYTYLDTFGRPVIVAYKKNLVEQHIQDIVVHYTFNKVLMLQEPLLVVAAFYILF
                     FTVIIYVRLDFSITKDPAAEARMKVACITEQVLTLVNKRIGLYRHFDETVNRYKQSRD
                     ISTLNSGKKSLETEHKALTSEIALLQSRLKTEGSDLCDRVSEMQKLDAQVKELVLKSA
                     VEAERLVAGKLKKDTYIENEKLISGKRQELVTKIDHILDAL"
     exon            280..344
                     /gene="RPN1"
                     /gene_synonym="OST1; RBPH1"
                     /inference="alignment:Splign:2.1.0"
     exon            345..651
                     /gene="RPN1"
                     /gene_synonym="OST1; RBPH1"
                     /inference="alignment:Splign:2.1.0"
     exon            652..861
                     /gene="RPN1"
                     /gene_synonym="OST1; RBPH1"
                     /inference="alignment:Splign:2.1.0"
     exon            862..1054
                     /gene="RPN1"
                     /gene_synonym="OST1; RBPH1"
                     /inference="alignment:Splign:2.1.0"
     exon            1055..1154
                     /gene="RPN1"
                     /gene_synonym="OST1; RBPH1"
                     /inference="alignment:Splign:2.1.0"
     exon            1155..1293
                     /gene="RPN1"
                     /gene_synonym="OST1; RBPH1"
                     /inference="alignment:Splign:2.1.0"
     exon            1294..1413
                     /gene="RPN1"
                     /gene_synonym="OST1; RBPH1"
                     /inference="alignment:Splign:2.1.0"
     exon            1414..1659
                     /gene="RPN1"
                     /gene_synonym="OST1; RBPH1"
                     /inference="alignment:Splign:2.1.0"
     exon            1660..2284
                     /gene="RPN1"
                     /gene_synonym="OST1; RBPH1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2074..2079
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RPN1"
                     /gene_synonym="OST1; RBPH1"
                     /note="hexamer: AACAAA"
     polyA_site      2105
                     /gene="RPN1"
                     /gene_synonym="OST1; RBPH1"
     regulatory      2260..2265
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RPN1"
                     /gene_synonym="OST1; RBPH1"
                     /note="hexamer: AATAAA"
     polyA_site      2284
                     /gene="RPN1"
                     /gene_synonym="OST1; RBPH1"
                     /note="major polyA site"
ORIGIN      
        1 gcactgctct tcccggtcat ggaggcgcca gccgccggct tgtttctgct cctgttgctt
       61 gggacttggg ccccggcgcc gggcagcgcc tcctccgagg caccgccgct gatcaatgag
      121 gacgtgaagc gcacagtgga cctaagcagc cacctggcta aggtgacggc cgaggtggtc
      181 ctggcgcacc tgggcggcgg ctccacgtcc cgagctacct ctttcctgct ggctttggag
      241 cctgagctcg aggcccggct ggcgcacctg ggcgtgcagg taaagggaga agatgaggaa
      301 gagaacaatt tggaagtacg tgaaaccaaa attaagggta aaagtgggag attcttcaca
      361 gtcaagctcc cagttgctct tgatcctggg gccaagattt cagtcattgt ggaaacagtc
      421 tacacccatg tgcttcatcc gtatccaacc cagatcaccc agtcagagaa acagtttgtg
      481 gtgtttgagg ggaaccatta tttctactct ccctatccaa cgaagacaca aaccatgcgt
      541 gtgaagcttg cctctcgaaa tgtggagagc tacaccaagc tggggaaccc cacgcgctct
      601 gaggacctac tggattatgg gcctttcaga gatgtgcctg cctatagtca ggatactttt
      661 aaagtacatt atgagaacaa cagccctttc ctgaccatca ccagcatgac ccgagtcatt
      721 gaagtctctc actggggtaa tattgctgtg gaagaaaatg tggacttaaa gcacacagga
      781 gctgtgctta aggggccttt ctcacgctat gattaccaga gacagccaga tagtggaata
      841 tcctccatcc gttcttttaa gaccatcctt cctgctgctg cccaggatgt ttattaccgg
      901 gatgagattg gcaatgtttc taccagccac ctccttattt tggatgactc tgtagagatg
      961 gaaatccggc ctcgcttccc tctctttggc gggtggaaga cccattacat cgttggctac
     1021 aacctcccaa gctatgagta cctctataat ttgggtgacc agtatgcact gaagatgagg
     1081 tttgtggacc atgtgtttga tgaacaagtg atagattctc tgactgtgaa gatcatcctg
     1141 cctgaaggag ccaagaacat tgaaattgat agtccctatg aaatcagccg tgccccagat
     1201 gagctgcact acacctatct ggacacattt ggccgccctg tgattgttgc ctacaagaaa
     1261 aatctggtag aacagcacat tcaggacatt gtggtccact acacgttcaa caaggtgctc
     1321 atgctgcagg agcccctgct ggtggtggcg gccttctaca tcctgttctt caccgttatc
     1381 atctatgttc ggctggactt ctccatcacc aaggatccag ccgcagaagc caggatgaag
     1441 gtagcctgca tcacagagca ggtcttgacc ctggtcaaca agagaatagg cctttaccgt
     1501 cactttgacg agaccgtcaa taggtacaag caatcccggg acatctccac cctcaacagt
     1561 ggcaagaaga gcctggagac tgaacacaag gccttgacca gtgagattgc actgctgcag
     1621 tccaggctga agacagaggg ctctgatctg tgcgacagag tgagcgaaat gcagaagctg
     1681 gatgcacagg tcaaggagct ggtgctgaag tcggcggtgg aggctgagcg cctggtggct
     1741 ggcaagctca agaaagacac gtacattgag aatgagaagc tcatctcagg aaagcgccag
     1801 gagctggtca ccaagatcga ccacatcctg gatgccctgt agcccctgcc cgcatcctcc
     1861 agggggccca gggtgcctgc actttgctgt ggcaggcaga ttgggtggta gtgggaggtt
     1921 gtgcatggag gccagtgaaa gctgacatct gtaaaaggcc ttcaaggaag agaaaccagg
     1981 ccctgcgtca ggcagtgtga gtttgccgtt tgtccttaac tttctttttt ttttttttta
     2041 aaaaaagaaa actttaaaaa aactcccatt aaaaacaaaa catctttgtg ttttgaacaa
     2101 aggaattttc aatatttgat tggtattctg ttctgaagtc taagatattt ttcagcctat
     2161 aaagccccct gttttatgcc cttctaattc tgatgtttgg gtattgtgtg agtgcatgtg
     2221 tttttttttt tttttttaaa gcgtgtgtga acaaatggaa ataaagcagg gactgtgaac
     2281 attt
//
