LOCUS       NM_000973                845 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens ribosomal protein L8 (RPL8), transcript variant 1,
            mRNA.
ACCESSION   NM_000973
VERSION     NM_000973.5
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Sun,T., Zhu,W., Ru,Q. and Zheng,Y.
  TITLE     Silencing RPL8 inhibits the progression of hepatocellular carcinoma
            by down-regulating the mTORC1 signalling pathway
  JOURNAL   Hum Cell 36 (2), 725-737 (2023)
   PUBMED   36577883
  REMARK    GeneRIF: Silencing RPL8 inhibits the progression of hepatocellular
            carcinoma by down-regulating the mTORC1 signalling pathway.
REFERENCE   2  (bases 1 to 845)
  AUTHORS   Fan,S., Zhang,S., Kong,D., Wang,H., Shi,Y., Wang,Z. and Zheng,H.
  TITLE     Integrative Multi-Omics Analysis of Identified Ferroptosis-Marker
            RPL8 as a Candidate Oncogene Correlates with Poor Prognosis and
            Immune Infiltration in Liver Cancer
  JOURNAL   Comb Chem High Throughput Screen 26 (7), 1298-1310 (2023)
   PUBMED   36017842
  REMARK    GeneRIF: Integrative Multi-Omics Analysis of Identified
            Ferroptosis-Marker RPL8 as a Candidate Oncogene Correlates with
            Poor Prognosis and Immune Infiltration in Liver Cancer.
REFERENCE   3  (bases 1 to 845)
  AUTHORS   Liang,X., Zuo,M.Q., Zhang,Y., Li,N., Ma,C., Dong,M.Q. and Gao,N.
  TITLE     Structural snapshots of human pre-60S ribosomal particles before
            and after nuclear export
  JOURNAL   Nat Commun 11 (1), 3542 (2020)
   PUBMED   32669547
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 845)
  AUTHORS   Kennedy,S.A., Jarboui,M.A., Srihari,S., Raso,C., Bryan,K.,
            Dernayka,L., Charitou,T., Bernal-Llinares,M., Herrera-Montavez,C.,
            Krstic,A., Matallanas,D., Kotlyar,M., Jurisica,I., Curak,J.,
            Wong,V., Stagljar,I., LeBihan,T., Imrie,L., Pillai,P., Lynn,M.A.,
            Fasterius,E., Al-Khalili Szigyarto,C., Breen,J., Kiel,C.,
            Serrano,L., Rauch,N., Rukhlenko,O., Kholodenko,B.N.,
            Iglesias-Martinez,L.F., Ryan,C.J., Pilkington,R., Cammareri,P.,
            Sansom,O., Shave,S., Auer,M., Horn,N., Klose,F., Ueffing,M.,
            Boldt,K., Lynn,D.J. and Kolch,W.
  TITLE     Extensive rewiring of the EGFR network in colorectal cancer cells
            expressing transforming levels of KRASG13D
  JOURNAL   Nat Commun 11 (1), 499 (2020)
   PUBMED   31980649
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 845)
  AUTHORS   Wang,C., Zhang,Q., Hang,T., Tao,Y., Ma,X., Wu,M., Zhang,X. and
            Zang,J.
  TITLE     Structure of the JmjC domain-containing protein NO66 complexed with
            ribosomal protein Rpl8
  JOURNAL   Acta Crystallogr D Biol Crystallogr 71 (Pt 9), 1955-1964 (2015)
   PUBMED   26327385
  REMARK    GeneRIF: confirmed the hydroxylase activity of NO66 and showed that
            oligomerization is required for NO66 to efficiently catalyze the
            hydroxylation of Rpl8
REFERENCE   6  (bases 1 to 845)
  AUTHORS   Andersen,J.S., Lyon,C.E., Fox,A.H., Leung,A.K., Lam,Y.W., Steen,H.,
            Mann,M. and Lamond,A.I.
  TITLE     Directed proteomic analysis of the human nucleolus
  JOURNAL   Curr Biol 12 (1), 1-11 (2002)
   PUBMED   11790298
REFERENCE   7  (bases 1 to 845)
  AUTHORS   Kenmochi,N., Kawaguchi,T., Rozen,S., Davis,E., Goodman,N.,
            Hudson,T.J., Tanaka,T. and Page,D.C.
  TITLE     A map of 75 human ribosomal protein genes
  JOURNAL   Genome Res 8 (5), 509-523 (1998)
   PUBMED   9582194
REFERENCE   8  (bases 1 to 845)
  AUTHORS   Uhlein,M., Weglohner,W., Urlaub,H. and Wittmann-Liebold,B.
  TITLE     Functional implications of ribosomal protein L2 in protein
            biosynthesis as shown by in vivo replacement studies
  JOURNAL   Biochem J 331 (Pt 2) (Pt 2), 423-430 (1998)
   PUBMED   9531480
REFERENCE   9  (bases 1 to 845)
  AUTHORS   Wool,I.G., Chan,Y.L. and Gluck,A.
  TITLE     Structure and evolution of mammalian ribosomal proteins
  JOURNAL   Biochem Cell Biol 73 (11-12), 933-947 (1995)
   PUBMED   8722009
  REMARK    Review article
REFERENCE   10 (bases 1 to 845)
  AUTHORS   Hanes,J., Klaudiny,J., von der Kammer,H. and Scheit,K.H.
  TITLE     Characterization by cDNA cloning of the mRNA of human ribosomal
            protein L8
  JOURNAL   Biochem Biophys Res Commun 197 (3), 1223-1228 (1993)
   PUBMED   7506540
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff in
            collaboration with Francesco Amaldi. The reference sequence was
            derived from BC093064.1.
            
            On Jun 2, 2019 this sequence version replaced NM_000973.4.
            
            Summary: Ribosomes, the organelles that catalyze protein synthesis,
            consist of a small 40S subunit and a large 60S subunit. Together
            these subunits are composed of 4 RNA species and approximately 80
            structurally distinct proteins. This gene encodes a ribosomal
            protein that is a component of the 60S subunit. The protein belongs
            to the L2P family of ribosomal proteins. It is located in the
            cytoplasm. In rat, the protein associates with the 5.8S rRNA, very
            likely participates in the binding of aminoacyl-tRNA, and is a
            constituent of the elongation factor 2-binding site at the
            ribosomal subunit interface. Alternatively spliced transcript
            variants encoding the same protein exist. As is typical for genes
            encoding ribosomal proteins, there are multiple processed
            pseudogenes of this gene dispersed through the genome. [provided by
            RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the shortest
            transcript. Variants 1-4 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CD051211.1, BI553837.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-845               BC093064.1         31-875
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q24.3"
     gene            1..845
                     /gene="RPL8"
                     /gene_synonym="L8; uL2"
                     /note="ribosomal protein L8"
                     /db_xref="GeneID:6132"
                     /db_xref="HGNC:HGNC:10368"
                     /db_xref="MIM:604177"
     exon            1..25
                     /gene="RPL8"
                     /gene_synonym="L8; uL2"
                     /inference="alignment:Splign:2.1.0"
     exon            26..174
                     /gene="RPL8"
                     /gene_synonym="L8; uL2"
                     /inference="alignment:Splign:2.1.0"
     CDS             37..810
                     /gene="RPL8"
                     /gene_synonym="L8; uL2"
                     /note="60S ribosomal protein L8"
                     /codon_start=1
                     /product="large ribosomal subunit protein uL2"
                     /protein_id="NP_000964.1"
                     /db_xref="CCDS:CCDS6433.1"
                     /db_xref="GeneID:6132"
                     /db_xref="HGNC:HGNC:10368"
                     /db_xref="MIM:604177"
                     /translation="MGRVIRGQRKGAGSVFRAHVKHRKGAARLRAVDFAERHGYIKGI
                     VKDIIHDPGRGAPLAKVVFRDPYRFKKRTELFIAAEGIHTGQFVYCGKKAQLNIGNVL
                     PVGTMPEGTIVCCLEEKPGDRGKLARASGNYATVISHNPETKKTRVKLPSGSKKVISS
                     ANRAVVGVVAGGGRIDKPILKAGRAYHKYKAKRNCWPRVRGVAMNPVEHPFGGGNHQH
                     IGKPSTIRRDAPAGRKVGLIAARRTGRLRGTKTVQEKEN"
     exon            175..316
                     /gene="RPL8"
                     /gene_synonym="L8; uL2"
                     /inference="alignment:Splign:2.1.0"
     exon            317..535
                     /gene="RPL8"
                     /gene_synonym="L8; uL2"
                     /inference="alignment:Splign:2.1.0"
     exon            536..651
                     /gene="RPL8"
                     /gene_synonym="L8; uL2"
                     /inference="alignment:Splign:2.1.0"
     exon            652..845
                     /gene="RPL8"
                     /gene_synonym="L8; uL2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      824..829
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RPL8"
                     /gene_synonym="L8; uL2"
                     /note="hexamer: AATAAA"
     polyA_site      845
                     /gene="RPL8"
                     /gene_synonym="L8; uL2"
                     /note="major polyA site"
ORIGIN      
        1 ctctttcggc cgcgctggtg aacaggaccc gtcgccatgg gccgtgtgat ccgtggacag
       61 aggaagggcg ccgggtctgt gttccgcgcg cacgtgaagc accgtaaagg cgctgcgcgc
      121 ctgcgcgccg tggatttcgc tgagcggcac ggctacatca agggcatcgt caaggacatc
      181 atccacgacc cgggccgcgg cgcgcccctc gccaaggtgg tcttccggga tccgtatcgg
      241 tttaagaagc ggacggagct gttcattgcc gccgagggca ttcacacggg ccagtttgtg
      301 tattgcggca agaaggccca gctcaacatt ggcaatgtgc tccctgtggg caccatgcct
      361 gagggtacaa tcgtgtgctg cctggaggag aagcctggag accgtggcaa gctggcccgg
      421 gcatcaggga actatgccac cgttatctcc cacaaccctg agaccaagaa gacccgtgtg
      481 aagctgccct ccggctccaa gaaggttatc tcctcagcca acagagctgt ggttggtgtg
      541 gtggctggag gtggccgaat tgacaaaccc atcttgaagg ctggccgggc gtaccacaaa
      601 tataaggcaa agaggaactg ctggccacga gtacggggtg tggccatgaa tcctgtggag
      661 catccttttg gaggtggcaa ccaccagcac atcggcaagc cctccaccat ccgcagagat
      721 gcccctgctg gccgcaaagt gggtctcatt gctgcccgcc ggactggacg tctccgggga
      781 accaagactg tgcaggagaa agagaactag tgctgagggc ctcaataaag tttgtgttta
      841 tgcca
//
