LOCUS       NM_000994               2094 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens ribosomal protein L32 (RPL32), transcript variant 1,
            mRNA.
ACCESSION   NM_000994
VERSION     NM_000994.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Xu,L., Wang,L., Jiang,C., Zhu,Q., Chen,R., Wang,J. and Wang,S.
  TITLE     Biological effect of ribosomal protein L32 on human breast cancer
            cell behavior
  JOURNAL   Mol Med Rep 22 (3), 2478-2486 (2020)
   PUBMED   32705264
  REMARK    GeneRIF: Biological effect of ribosomal protein L32 on human breast
            cancer cell behavior.
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Liang,X., Zuo,M.Q., Zhang,Y., Li,N., Ma,C., Dong,M.Q. and Gao,N.
  TITLE     Structural snapshots of human pre-60S ribosomal particles before
            and after nuclear export
  JOURNAL   Nat Commun 11 (1), 3542 (2020)
   PUBMED   32669547
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2094)
  AUTHORS   Rutkowski,D., Syed,F., Matthews,L.C., Ray,D.W., McGrouther,D.A.,
            Watson,R.E. and Bayat,A.
  TITLE     An abnormality in glucocorticoid receptor expression differentiates
            steroid responders from nonresponders in keloid disease
  JOURNAL   Br J Dermatol 173 (3), 690-700 (2015)
   PUBMED   25712143
REFERENCE   4  (bases 1 to 2094)
  AUTHORS   Behrmann,E., Loerke,J., Budkevich,T.V., Yamamoto,K., Schmidt,A.,
            Penczek,P.A., Vos,M.R., Burger,J., Mielke,T., Scheerer,P. and
            Spahn,C.M.
  TITLE     Structural snapshots of actively translating human ribosomes
  JOURNAL   Cell 161 (4), 845-857 (2015)
   PUBMED   25957688
REFERENCE   5  (bases 1 to 2094)
  AUTHORS   Khatter,H., Myasnikov,A.G., Natchiar,S.K. and Klaholz,B.P.
  TITLE     Structure of the human 80S ribosome
  JOURNAL   Nature 520 (7549), 640-645 (2015)
   PUBMED   25901680
REFERENCE   6  (bases 1 to 2094)
  AUTHORS   Yoshihama,M., Uechi,T., Asakawa,S., Kawasaki,K., Kato,S., Higa,S.,
            Maeda,N., Minoshima,S., Tanaka,T., Shimizu,N. and Kenmochi,N.
  TITLE     The human ribosomal protein genes: sequencing and comparative
            analysis of 73 genes
  JOURNAL   Genome Res 12 (3), 379-390 (2002)
   PUBMED   11875025
REFERENCE   7  (bases 1 to 2094)
  AUTHORS   Kenmochi,N., Kawaguchi,T., Rozen,S., Davis,E., Goodman,N.,
            Hudson,T.J., Tanaka,T. and Page,D.C.
  TITLE     A map of 75 human ribosomal protein genes
  JOURNAL   Genome Res 8 (5), 509-523 (1998)
   PUBMED   9582194
REFERENCE   8  (bases 1 to 2094)
  AUTHORS   Vorobieva,N.V., Filipenko,M.L., Karpova,G.G., Mertvetsov,N.P. and
            Graphodatsky,A.S.
  TITLE     Assignment of the L32 ribosomal protein gene (RPL32) to human
            chromosome 3q13.3-->q21 by in situ hybridization
  JOURNAL   Cytogenet Cell Genet 77 (3-4), 190-191 (1997)
   PUBMED   9284913
REFERENCE   9  (bases 1 to 2094)
  AUTHORS   Wool,I.G., Chan,Y.L. and Gluck,A.
  TITLE     Structure and evolution of mammalian ribosomal proteins
  JOURNAL   Biochem Cell Biol 73 (11-12), 933-947 (1995)
   PUBMED   8722009
  REMARK    Review article
REFERENCE   10 (bases 1 to 2094)
  AUTHORS   Young,J.A. and Trowsdale,J.
  TITLE     A processed pseudogene in an intron of the HLA-DP beta 1 chain gene
            is a member of the ribosomal protein L32 gene family
  JOURNAL   Nucleic Acids Res 13 (24), 8883-8891 (1985)
   PUBMED   3866218
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff in
            collaboration with Francesco Amaldi. The reference sequence was
            derived from CF124158.1, AC034198.7 and CA436299.1.
            
            On May 17, 2019 this sequence version replaced NM_000994.3.
            
            Summary: Ribosomes, the organelles that catalyze protein synthesis,
            consist of a small 40S subunit and a large 60S subunit. Together
            these subunits are composed of 4 RNA species and approximately 80
            structurally distinct proteins. This gene encodes a ribosomal
            protein that is a component of the 60S subunit. The protein belongs
            to the L32E family of ribosomal proteins. It is located in the
            cytoplasm. Although some studies have mapped this gene to
            3q13.3-q21, it is believed to map to 3p25-p24. As is typical for
            genes encoding ribosomal proteins, there are multiple processed
            pseudogenes of this gene dispersed through the genome.
            Alternatively spliced transcript variants encoding the same protein
            have been observed for this gene. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the predominant
            transcript. Variants 1, 2 and 3 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BX419829.2, BX432517.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000429711.7/ ENSP00000416429.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-29                CF124158.1         55-83
            30-72               AC034198.7         39815-39857
            73-173              AC034198.7         41109-41209
            174-355             AC034198.7         41821-42002
            356-1568            AC034198.7         45128-46340
            1569-1634           CA436299.1         18-83               c
            1635-2094           AC034198.7         46407-46866
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p25.2"
     gene            1..2094
                     /gene="RPL32"
                     /gene_synonym="eL32; L32; PP9932"
                     /note="ribosomal protein L32"
                     /db_xref="GeneID:6161"
                     /db_xref="HGNC:HGNC:10336"
     exon            1..72
                     /gene="RPL32"
                     /gene_synonym="eL32; L32; PP9932"
                     /inference="alignment:Splign:2.1.0"
     exon            73..173
                     /gene="RPL32"
                     /gene_synonym="eL32; L32; PP9932"
                     /inference="alignment:Splign:2.1.0"
     CDS             78..485
                     /gene="RPL32"
                     /gene_synonym="eL32; L32; PP9932"
                     /note="60S ribosomal protein L32"
                     /codon_start=1
                     /product="large ribosomal subunit protein eL32"
                     /protein_id="NP_000985.1"
                     /db_xref="CCDS:CCDS2614.1"
                     /db_xref="GeneID:6161"
                     /db_xref="HGNC:HGNC:10336"
                     /translation="MAALRPLVKPKIVKKRTKKFIRHQSDRYVKIKRNWRKPRGIDNR
                     VRRRFKGQILMPNIGYGSNKKTKHMLPSGFRKFLVHNVKELEVLLMCNKSYCAEIAHN
                     VSSKNRKAIVERAAQLAIRVTNPNARLRSEENE"
     exon            174..355
                     /gene="RPL32"
                     /gene_synonym="eL32; L32; PP9932"
                     /inference="alignment:Splign:2.1.0"
     exon            356..2094
                     /gene="RPL32"
                     /gene_synonym="eL32; L32; PP9932"
                     /inference="alignment:Splign:2.1.0"
     regulatory      513..518
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RPL32"
                     /gene_synonym="eL32; L32; PP9932"
                     /note="hexamer: AATAAA"
     polyA_site      532
                     /gene="RPL32"
                     /gene_synonym="eL32; L32; PP9932"
                     /note="major polyA site"
     regulatory      1541..1546
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RPL32"
                     /gene_synonym="eL32; L32; PP9932"
                     /note="hexamer: AATAAA"
     polyA_site      1566
                     /gene="RPL32"
                     /gene_synonym="eL32; L32; PP9932"
     regulatory      1607..1612
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RPL32"
                     /gene_synonym="eL32; L32; PP9932"
                     /note="hexamer: CATAAA"
     polyA_site      1627
                     /gene="RPL32"
                     /gene_synonym="eL32; L32; PP9932"
ORIGIN      
        1 agcccttgcg cgccaccgtc ccttctctct tcctcggcgc tgcctacgga ggtggcagcc
       61 atctccttct cggcatcatg gccgccctca gaccccttgt gaagcccaag atcgtcaaaa
      121 agagaaccaa gaagttcatc cggcaccagt cagaccgata tgtcaaaatt aagcgtaact
      181 ggcggaaacc cagaggcatt gacaacaggg ttcgtagaag attcaagggc cagatcttga
      241 tgcccaacat tggttatgga agcaacaaaa aaacaaagca catgctgccc agtggcttcc
      301 ggaagttcct ggtccacaac gtcaaggagc tggaagtgct gctgatgtgc aacaaatctt
      361 actgtgccga gatcgctcac aatgtttcct ccaagaaccg caaagccatc gtggaaagag
      421 ctgcccaact ggccatcaga gtcaccaacc ccaatgccag gctgcgcagt gaagaaaatg
      481 agtaggcagc tcatgtgcac gttttctgtt taaataaatg taaaaactgc catctggcat
      541 cttccttcct tgattttaag tcttcagctt cttggccaac ttagtttgcc acagagattg
      601 ttcttttgct taagcccctt tggaatctcc catttggagg ggatttgtaa aggacactca
      661 gtccttgaac aggggaatgt ggcctcaagt gcacagacta gccttagtca tctccagttg
      721 aggctgggta tgaggggtac agacttggcc ctcacaccag gtaggttctg agacacttga
      781 agaagcttgt ggctcccaag ccacaagtag tcattcttag ccttgctttt gtaaagttag
      841 gtgacaagtt attccatgtg atgcttgtga gaattgagaa aatatgcatg gaaatatcca
      901 gatgaatttc ttacacagat tcttacggga tgcctaaatt gcatcctgta acttctgtcc
      961 aaaaagaaca ggatgatgta caaattgctc ttccaggtaa tccaccacgg ttaactggaa
     1021 aagcactttc agtctcctat aaccctccca ccagctgctg cttcaggtat aatgttacag
     1081 cagtttgcca aggcggggac ctaactggtg acaattgagc ctcttgactg gtactcagaa
     1141 tttagtgaca cgtggtcctg attttttttg gagacggggt cttgctctca cccaggctgg
     1201 gagtgcagtg gcacactgac tacagccttg acctccccag gctcaggtga tcttcccacc
     1261 tcagccttcc aagtagctgg gactacagat gcacacctcc aaacctgggt agtttttgaa
     1321 gtttttttgt agaggtggtc tagccatgtt gcctaggctc ccgaactcct gagctcaagc
     1381 aatcctgctt cagcctccca aagtactggg attacaggca tcttctgtag tatataggtc
     1441 atgagggata tgggatgtgg tacttatgag acagaaatgc ttacaggatg tttttctgta
     1501 accatcctgg tcaacttagc agaaatgctg cgctgggtat aataaagctt ttctacttct
     1561 agtctagaca ggaatcttac agattgtctc ctgttcaaaa cctagtcata aatatttata
     1621 atgcaaactg gtcatttttg ggattttgtt tttgagactc attctgtcac cccggctgga
     1681 gtgcagtggc ttgatcttgg ctccctgcaa cttctgcctc ctgggttcag gcgattcgat
     1741 taacaattgg agacgagtcg tgcagaggaa agaagtaact tagaatctaa tctttattaa
     1801 cctgggatat cacagaaaat acttgggcct gccacttgta tttctttgtc ctcaatactg
     1861 aacactagac acatttgaaa ccttcctcag aaggaaacaa accaactggt tatgcctggg
     1921 tggtggcatg gaagatgccc attgttgccc aatttgctaa taagtatttt gaaattagaa
     1981 atgaaacatg aggtccaagc ctgtccagtt ccctacccaa cccattcctc ctccctggaa
     2041 aaaagaaacc cgtatcatct tccaaatttc tgattcatca ctgccactta aaaa
//
