LOCUS       NM_000991               4209 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens ribosomal protein L28 (RPL28), transcript variant 2,
            mRNA.
ACCESSION   NM_000991
VERSION     NM_000991.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 4209)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 4209)
  AUTHORS   Liang,X., Zuo,M.Q., Zhang,Y., Li,N., Ma,C., Dong,M.Q. and Gao,N.
  TITLE     Structural snapshots of human pre-60S ribosomal particles before
            and after nuclear export
  JOURNAL   Nat Commun 11 (1), 3542 (2020)
   PUBMED   32669547
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4209)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 4209)
  AUTHORS   Labriet,A., Levesque,E., Cecchin,E., De Mattia,E., Villeneuve,L.,
            Rouleau,M., Jonker,D., Couture,F., Simonyan,D., Allain,E.P.,
            Buonadonna,A., D'Andrea,M., Toffoli,G. and Guillemette,C.
  TITLE     Germline variability and tumor expression level of ribosomal
            protein gene RPL28 are associated with survival of metastatic
            colorectal cancer patients
  JOURNAL   Sci Rep 9 (1), 13008 (2019)
   PUBMED   31506518
  REMARK    GeneRIF: Germline variability and tumor expression level of
            ribosomal protein gene RPL28 are associated with survival of
            metastatic colorectal cancer patients.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4209)
  AUTHORS   Kenmochi,N., Kawaguchi,T., Rozen,S., Davis,E., Goodman,N.,
            Hudson,T.J., Tanaka,T. and Page,D.C.
  TITLE     A map of 75 human ribosomal protein genes
  JOURNAL   Genome Res 8 (5), 509-523 (1998)
   PUBMED   9582194
REFERENCE   7  (bases 1 to 4209)
  AUTHORS   Wool,I.G., Chan,Y.L. and Gluck,A.
  TITLE     Structure and evolution of mammalian ribosomal proteins
  JOURNAL   Biochem Cell Biol 73 (11-12), 933-947 (1995)
   PUBMED   8722009
  REMARK    Review article
REFERENCE   8  (bases 1 to 4209)
  AUTHORS   Frigerio,J.M., Dagorn,J.C. and Iovanna,J.L.
  TITLE     Cloning, sequencing and expression of the L5, L21, L27a, L28, S5,
            S9, S10 and S29 human ribosomal protein mRNAs
  JOURNAL   Biochim Biophys Acta 1262 (1), 64-68 (1995)
   PUBMED   7772601
REFERENCE   9  (bases 1 to 4209)
  AUTHORS   Kato,S., Sekine,S., Oh,S.W., Kim,N.S., Umezawa,Y., Abe,N.,
            Yokoyama-Kobayashi,M. and Aoki,T.
  TITLE     Construction of a human full-length cDNA bank
  JOURNAL   Gene 150 (2), 243-250 (1994)
   PUBMED   7821789
REFERENCE   10 (bases 1 to 4209)
  AUTHORS   Brown,J.R., Daar,I.O., Krug,J.R. and Maquat,L.E.
  TITLE     Characterization of the functional gene and several processed
            pseudogenes in the human triosephosphate isomerase gene family
  JOURNAL   Mol Cell Biol 5 (7), 1694-1706 (1985)
   PUBMED   4022011
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff in
            collaboration with Francesco Amaldi. The reference sequence was
            derived from AK311760.1, AC020922.9 and BC011582.1.
            
            On Jun 2, 2019 this sequence version replaced NM_000991.4.
            
            Summary: Ribosomes, the organelles that catalyze protein synthesis,
            consist of a small 40S subunit and a large 60S subunit. Together
            these subunits are composed of 4 RNA species and approximately 80
            structurally distinct proteins. This gene encodes a ribosomal
            protein that is a component of the 60S subunit. The protein belongs
            to the L28E family of ribosomal proteins. It is located in the
            cytoplasm. Variable expression of this gene in colorectal cancers
            compared to adjacent normal tissues has been observed, although no
            correlation between the level of expression and the severity of the
            disease has been found. As is typical for genes encoding ribosomal
            proteins, there are multiple processed pseudogenes of this gene
            dispersed through the genome. Alternative splicing results in
            multiple transcript variants encoding distinct isoforms.[provided
            by RefSeq, Oct 2008].
            
            Transcript Variant: This variant (2) lacks a stop-codon-containing
            segment in the 3' coding region, compared to variant 1. The encoded
            protein (isoform 2) has a distinct and shorter C-terminus when
            compared to isoform 1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic and transcript sequence to make the sequence consistent
            with the reference genome assembly. The extent of this transcript
            is supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC011582.1, AU131942.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000344063.7/ ENSP00000342787.3
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-456               AK311760.1         1-456
            457-4195            AC020922.9         96616-100354
            4196-4209           BC011582.1         4173-4186
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.42"
     gene            1..4209
                     /gene="RPL28"
                     /gene_synonym="eL28; L28"
                     /note="ribosomal protein L28"
                     /db_xref="GeneID:6158"
                     /db_xref="HGNC:HGNC:10330"
                     /db_xref="MIM:603638"
     exon            1..34
                     /gene="RPL28"
                     /gene_synonym="eL28; L28"
                     /inference="alignment:Splign:2.1.0"
     exon            35..123
                     /gene="RPL28"
                     /gene_synonym="eL28; L28"
                     /inference="alignment:Splign:2.1.0"
     CDS             43..456
                     /gene="RPL28"
                     /gene_synonym="eL28; L28"
                     /EC_number="3.6.5.3"
                     /note="isoform 2 is encoded by transcript variant 2; 60S
                     ribosomal protein L28; large ribosomal subunit protein
                     eL28"
                     /codon_start=1
                     /product="large ribosomal subunit protein eL28 isoform 2"
                     /protein_id="NP_000982.2"
                     /db_xref="CCDS:CCDS12924.1"
                     /db_xref="GeneID:6158"
                     /db_xref="HGNC:HGNC:10330"
                     /db_xref="MIM:603638"
                     /translation="MSAHLQWMVVRNCSSFLIKRNKQTYSTEPNNLKARNSFRYNGLI
                     HRKTVGVEPAADGKGVVVVIKRRSGQRKPATSYVRTTINKNARATLSSIRHMIRKNKY
                     RPDLRMAAIRRASAILRSQKPVMVKRKRTRPTKSS"
     exon            124..247
                     /gene="RPL28"
                     /gene_synonym="eL28; L28"
                     /inference="alignment:Splign:2.1.0"
     exon            248..366
                     /gene="RPL28"
                     /gene_synonym="eL28; L28"
                     /inference="alignment:Splign:2.1.0"
     exon            367..4209
                     /gene="RPL28"
                     /gene_synonym="eL28; L28"
                     /inference="alignment:Splign:2.1.0"
     regulatory      475..480
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RPL28"
                     /gene_synonym="eL28; L28"
                     /note="hexamer: AATAAA"
     polyA_site      499
                     /gene="RPL28"
                     /gene_synonym="eL28; L28"
                     /note="major polyA site"
     regulatory      3871..3876
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RPL28"
                     /gene_synonym="eL28; L28"
                     /note="hexamer: AATAAA"
     polyA_site      3896
                     /gene="RPL28"
                     /gene_synonym="eL28; L28"
     regulatory      4188..4193
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RPL28"
                     /gene_synonym="eL28; L28"
                     /note="hexamer: AATAAA"
     polyA_site      4209
                     /gene="RPL28"
                     /gene_synonym="eL28; L28"
ORIGIN      
        1 ctctttccgt ctcaggtcgc cgctgcgaag ggagccgccg ccatgtctgc gcatctgcaa
       61 tggatggtcg tgcggaactg ctccagtttc ctgatcaaga ggaataagca gacctacagc
      121 actgagccca ataacttgaa ggcccgcaat tccttccgct acaacggact gattcaccgc
      181 aagactgtgg gcgtggagcc ggcagccgac ggcaaaggtg tcgtggtggt cattaagcgg
      241 agatccggcc agcggaagcc tgccacctcc tatgtgcgga ccaccatcaa caagaatgct
      301 cgcgccacgc tcagcagcat cagacacatg atccgcaaga acaagtaccg ccccgacctg
      361 cgcatggcag ccatccgcag ggccagcgcc atcctgcgca gccagaagcc tgtgatggtg
      421 aagaggaagc ggacccgccc caccaagagc tcctgagccc cctgccccca gagcaataaa
      481 gtcagctggc tttctcacct gcctcgactg ggcctccctt tttgaaacgc tctggggagc
      541 tctggccctg tgtgttgtca ttcaggccat gtcatcaaaa ctctgcatgt caccttgtcc
      601 atctggaggt gatgtcaatg gctggccatg caggaggggt ggggtagctg ccttgtccct
      661 ggtgagggca agggtcactg tcttcacaga aaaagtttgc tgacttgtga ttgagaccta
      721 ctgtcccatt gtgaggtggc ctgaagaatc ccagctgggg cagtggcttc cattcagaag
      781 aagaaaggcc ttttctagcc cagaagggtg caggctgagg gctgggccct gggccctggt
      841 gctgtagcac ggtttgggga cttggggtgt tcccaagacc tgggggacga cagacatcac
      901 gggaggaaga tgagatgact tttgcatcca gggagtgggt gcagccacat ttggagggga
      961 tgggctttac ttgatgcaac ctcatctctg agatgggcaa cttggtgggt ggtggcttat
     1021 aactgtaagg gagatggcag ccccagggta cagccagcag gcattgagca gccttagcat
     1081 tgtcccccta ctcccgtcct ccaggtgtcc ccatccctcc cctgtctctt tgagctggct
     1141 cttgtcactt aggtctcatc tcagtggccg ctcctgggcc accctgtcac ccaagctttc
     1201 ctgattgccc agccctcttg tttcctttgg cctgtttgct ccctagtgtt tattacagct
     1261 tgtgaggcca ggagtttgag accatcctag gcaacataat gagacaccgt ctctaaaata
     1321 aaattagctg ggtgtggtgg tgcaccgcct gtggtcccag ctcctcagag gttgagtaga
     1381 ggctgaggtg agcggagcac ttgagccaag agtatgaggc tgcagtgagc ccatgagccc
     1441 caccactaca ctccagcctg gaagacacca tgacacacag tgaggcctgg atggggaaag
     1501 agtcctgctg ttgatcctca catgtttcct gggcacctaa ctctgtcagc cactgccagg
     1561 gaccaaggat ccagcatcca tggcacccct ggttcctgcc atcctggggt acccgattca
     1621 aagaaggact ctgctccctg tctgagacca cccccggctc tgactgagag taaggggact
     1681 gtcagggcct cgacttgcca ttggttgggg tcgtacgggg ctgggagccc tgcgttttga
     1741 ggcagaccac tgcccttccg acctcagtcc tgtctgctcc agtcttgccc agctcgaagg
     1801 agagcagatc tgaccacttg ccagcccctg tctgctgtga attaccattt cctttgtcct
     1861 tcccttagtt gggtctatta gctcagattg agaggtgttg ccttaaaact gagttgggtg
     1921 acttggtacc tgctcaggac cccccgcact gtcccaatcc cactcaggcc cacctccagc
     1981 tggcctcact ccgctggtga cttcgtacct gctcaggagc ccccactgtc ccagtcccac
     2041 tcaggcccat ctctggctgg cctcactgcg ctgggactcc gccttcataa ggagagctca
     2101 ctgctcacgt tagtagatgg ccccttctcg tgaggcctct cccctggcac ctgcttcagt
     2161 tgtcctccac agcactgatt tgcagcccac aagctggcag gtttatctgt ctcatgtttg
     2221 tcttgtgctg gtgggcaagg ggtttgtcta gcacaccagc atataatgag atgcttgatg
     2281 aatggtgcat attgaatgta taaagcccac cggtcctgag agtttgctca ctggagactt
     2341 tctggagatg gagtctcgct ctgttgccca ggctggcgag tgcaatggcg cgatcttggc
     2401 tcactgcagc ctccacctcc tgggttcaag cgattctcct gcctcagcct cccgagtagc
     2461 tgggattaca ggtgggtgtc accacaccca gctcagtatt gtatttttag cagagatggg
     2521 gtttcaccat tttgcccagg ctggtttgga actcctgact tcaaattacc cacctgcctc
     2581 agcctcccaa agtgctggca ttacaggcgc tcgaggcttt ctgatgtggc tgctgctgct
     2641 cagaaggcct tgtccttaac cacctccttg cctgccctgg aggcttgtgc ctctaggccc
     2701 caccccctgt ggagtcctgc tggctttctc catccctatc tgaatcctcc ctgctgtgtg
     2761 gcctcccctg gtctcatccg taacacagcc cagcttagtg ggcctctgtt cctgcgggtg
     2821 gccagcctgt ctgtgtggct gggctgggga ggccacgtct ggtatctgaa tgctatcggt
     2881 gggttggggt ggaggaacca ggagagggct ggagggaggg agatggtctc agccccacag
     2941 agtttggagt cctcagtgtg ctgagcaaac gtggagacac catttccctc ctctagacct
     3001 catcttggag agagagatgt tggatggggc catctattcc agctttattc acacaaatca
     3061 tgtctgttgg cctggaaatt ggaaaaccag ttaaaccaaa aacatgatat taagaaaaca
     3121 ggcaggctca ccatagtaaa aatgctgaaa gccaaagaca aaattgggag aacaaaagaa
     3181 aagcgtcttg tcacatacag aaggtccctg ataaagttag tagctgccct catcagaaac
     3241 caggcccagg cagtggggac acatccagag tgctgaaaga acctccccca ggtcatccta
     3301 tccccaagag tgatgcccgg cagcattccc agctcagggc taatggttca cggaagccag
     3361 gaatcaaact gcctgggttc cagtcccagc tctgccagtt atgcccagct gtggggactt
     3421 gggcagctcg tttagtagca ccgtgcctca gtttcccata tgtaaaaggc cattttgagt
     3481 gcctttcaca gccctgcata aggcaggtgt ctcagtgttc actgctgtct ctccagctct
     3541 tagtccagta gctgcatggt gagtgagcgt agggcgcacc ctggaaggct gccaagccca
     3601 aagttgtgca gagcgctggg gactccagac tccccacagc agcagagact cgggactgag
     3661 gcatcctctg ttcacaggac atgctggcat ctactgggtc agggctctgc tgctcggtgg
     3721 ctgtgcaacc ttgggcaagt tcctcaacct ctctgtgtct tcgtaccctc atctgtaaca
     3781 tgcgtgtcga tagaccctac tactcagggt tgatgagaag attaaatgtg caaaacctgc
     3841 ttgactgtgc ccacaaatcc tgattgtagg aataaattaa tgacttttta taaatatttt
     3901 gatcagatgg actcatgatc acagatgtct tcacatgcct atgactaatt tgtacacaaa
     3961 ctaatgctcg tgtttcccaa gcacctggaa gacatgccag atccatgtgc agtaatgcct
     4021 ggtggctcca ggtctgcccc gccgtcctgt ggggctgtga gctttcccag cctcctgccc
     4081 gtgtttgtga atatcattct gtcctcagct gcatttccag cccaggctgt ttggcgctgc
     4141 ccaggaatgg tatcaattcc cctgtttctc ttgtagccag ttactagaat aaaatcatct
     4201 actttaaaa
//
