LOCUS       NM_000976                634 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens ribosomal protein L12 (RPL12), mRNA.
ACCESSION   NM_000976
VERSION     NM_000976.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 634)
  AUTHORS   Imami,K., Milek,M., Bogdanow,B., Yasuda,T., Kastelic,N., Zauber,H.,
            Ishihama,Y., Landthaler,M. and Selbach,M.
  TITLE     Phosphorylation of the Ribosomal Protein RPL12/uL11 Affects
            Translation during Mitosis
  JOURNAL   Mol Cell 72 (1), 84-98 (2018)
   PUBMED   30220558
  REMARK    GeneRIF: Phosphoproteomics on the fractions reveals that
            phosphorylation of serine 38 in RPL12/uL11, a known mitotic CDK1
            substrate, is strongly depleted in polysomes.
REFERENCE   3  (bases 1 to 634)
  AUTHORS   Veit,G., Oliver,K., Apaja,P.M., Perdomo,D., Bidaud-Meynard,A.,
            Lin,S.T., Guo,J., Icyuz,M., Sorscher,E.J., Hartman,J.L. IV and
            Lukacs,G.L.
  TITLE     Ribosomal Stalk Protein Silencing Partially Corrects the
            DeltaF508-CFTR Functional Expression Defect
  JOURNAL   PLoS Biol 14 (5), e1002462 (2016)
   PUBMED   27168400
  REMARK    GeneRIF: RPL12 role in CFTR protein folding and stability
            Erratum:[PLoS Biol. 2016 Nov 2;14(11):e1002574. doi:
            10.1371/journal.pbio.1002574.. PMID: 27806038]
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 634)
  AUTHORS   Khatter,H., Myasnikov,A.G., Natchiar,S.K. and Klaholz,B.P.
  TITLE     Structure of the human 80S ribosome
  JOURNAL   Nature 520 (7549), 640-645 (2015)
   PUBMED   25901680
REFERENCE   5  (bases 1 to 634)
  AUTHORS   Zanivan,S., Maione,F., Hein,M.Y., Hernandez-Fernaud,J.R.,
            Ostasiewicz,P., Giraudo,E. and Mann,M.
  TITLE     SILAC-based proteomics of human primary endothelial cell
            morphogenesis unveils tumor angiogenic markers
  JOURNAL   Mol Cell Proteomics 12 (12), 3599-3611 (2013)
   PUBMED   23979707
REFERENCE   6  (bases 1 to 634)
  AUTHORS   Ajuh,P., Kuster,B., Panov,K., Zomerdijk,J.C., Mann,M. and
            Lamond,A.I.
  TITLE     Functional analysis of the human CDC5L complex and identification
            of its components by mass spectrometry
  JOURNAL   EMBO J 19 (23), 6569-6581 (2000)
   PUBMED   11101529
REFERENCE   7  (bases 1 to 634)
  AUTHORS   Kenmochi,N., Kawaguchi,T., Rozen,S., Davis,E., Goodman,N.,
            Hudson,T.J., Tanaka,T. and Page,D.C.
  TITLE     A map of 75 human ribosomal protein genes
  JOURNAL   Genome Res 8 (5), 509-523 (1998)
   PUBMED   9582194
REFERENCE   8  (bases 1 to 634)
  AUTHORS   Uchiumi,T. and Kominami,R.
  TITLE     Binding of mammalian ribosomal protein complex P0.P1.P2 and protein
            L12 to the GTPase-associated domain of 28 S ribosomal RNA and
            effect on the accessibility to anti-28 S RNA autoantibody
  JOURNAL   J Biol Chem 272 (6), 3302-3308 (1997)
   PUBMED   9013569
REFERENCE   9  (bases 1 to 634)
  AUTHORS   Wool,I.G., Chan,Y.L. and Gluck,A.
  TITLE     Structure and evolution of mammalian ribosomal proteins
  JOURNAL   Biochem Cell Biol 73 (11-12), 933-947 (1995)
   PUBMED   8722009
  REMARK    Review article
REFERENCE   10 (bases 1 to 634)
  AUTHORS   Chu,W., Presky,D.H., Swerlick,R.A. and Burns,D.K.
  TITLE     The primary structure of human ribosomal protein L12
  JOURNAL   Nucleic Acids Res 21 (3), 749 (1993)
   PUBMED   8441690
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff in
            collaboration with Francesco Amaldi. The reference sequence was
            derived from CB155565.1, BC105603.1 and BC050644.1.
            
            On Nov 22, 2018 this sequence version replaced NM_000976.3.
            
            Summary: Ribosomes, the organelles that catalyze protein synthesis,
            consist of a small 40S subunit and a large 60S subunit. Together
            these subunits are composed of 4 RNA species and approximately 80
            structurally distinct proteins. This gene encodes a ribosomal
            protein that is a component of the 60S subunit. The protein belongs
            to the L11P family of ribosomal proteins. It is located in the
            cytoplasm. The protein binds directly to the 26S rRNA. This gene is
            co-transcribed with the U65 snoRNA, which is located in its fourth
            intron. As is typical for genes encoding ribosomal proteins, there
            are multiple processed pseudogenes of this gene dispersed through
            the genome. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189655.194580.1,
                                           SRR5189661.43552.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA2150385
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000361436.10/ ENSP00000354739.5
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-335               CB155565.1         27-361
            336-627             BC105603.1         339-630
            628-634             BC050644.1         607-613
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q33.3"
     gene            1..634
                     /gene="RPL12"
                     /gene_synonym="L12; uL11"
                     /note="ribosomal protein L12"
                     /db_xref="GeneID:6136"
                     /db_xref="HGNC:HGNC:10302"
                     /db_xref="MIM:180475"
     exon            1..126
                     /gene="RPL12"
                     /gene_synonym="L12; uL11"
                     /inference="alignment:Splign:2.1.0"
     CDS             90..587
                     /gene="RPL12"
                     /gene_synonym="L12; uL11"
                     /note="60S ribosomal protein L12"
                     /codon_start=1
                     /product="large ribosomal subunit protein uL11"
                     /protein_id="NP_000967.1"
                     /db_xref="CCDS:CCDS6872.1"
                     /db_xref="GeneID:6136"
                     /db_xref="HGNC:HGNC:10302"
                     /db_xref="MIM:180475"
                     /translation="MPPKFDPNEIKVVYLRCTGGEVGATSALAPKIGPLGLSPKKVGD
                     DIAKATGDWKGLRITVKLTIQNRQAQIEVVPSASALIIKALKEPPRDRKKQKNIKHSG
                     NITFDEIVNIARQMRHRSLARELSGTIKEILGTAQSVGCNVDGRHPHDIIDDINSGAV
                     ECPAS"
     exon            127..200
                     /gene="RPL12"
                     /gene_synonym="L12; uL11"
                     /inference="alignment:Splign:2.1.0"
     exon            201..299
                     /gene="RPL12"
                     /gene_synonym="L12; uL11"
                     /inference="alignment:Splign:2.1.0"
     exon            300..381
                     /gene="RPL12"
                     /gene_synonym="L12; uL11"
                     /inference="alignment:Splign:2.1.0"
     exon            382..468
                     /gene="RPL12"
                     /gene_synonym="L12; uL11"
                     /inference="alignment:Splign:2.1.0"
     exon            469..581
                     /gene="RPL12"
                     /gene_synonym="L12; uL11"
                     /inference="alignment:Splign:2.1.0"
     exon            582..634
                     /gene="RPL12"
                     /gene_synonym="L12; uL11"
                     /inference="alignment:Splign:2.1.0"
     regulatory      607..612
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RPL12"
                     /gene_synonym="L12; uL11"
                     /note="hexamer: AATAAA"
     polyA_site      634
                     /gene="RPL12"
                     /gene_synonym="L12; uL11"
                     /note="major polyA site"
ORIGIN      
        1 cctctcggct ttcggctcgg aggaggccaa ggtgcaactt ccttcggtcg tcccgaatcc
       61 gggttcatcc gacaccagcc gcctccacca tgccgccgaa gttcgacccc aacgagatca
      121 aagtcgtata cctgaggtgc accggaggtg aagtcggtgc cacttctgcc ctggccccca
      181 agatcggccc cctgggtctg tctccaaaaa aagttggtga tgacattgcc aaggcaacgg
      241 gtgactggaa gggcctgagg attacagtga aactgaccat tcagaacaga caggcccaga
      301 ttgaggtggt gccttctgcc tctgccctga tcatcaaagc cctcaaggaa ccaccaagag
      361 acagaaagaa acagaaaaac attaaacaca gtgggaatat cacttttgat gagattgtca
      421 acattgctcg acagatgcgg caccgatcct tagccagaga actctctgga accattaaag
      481 agatcctggg gactgcccag tcagtgggct gtaatgttga tggccgccat cctcatgaca
      541 tcatcgatga catcaacagt ggtgctgtgg aatgcccagc cagttaagca caaaggaaaa
      601 catttcaata aaggatcatt tgacaactgg tgga
//
