LOCUS       NM_002947               2020 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens replication protein A3 (RPA3), mRNA.
ACCESSION   NM_002947 XM_005249821
VERSION     NM_002947.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Liang,L., Zhao,Z., Jin,Q., Zhang,S., Zhao,Z. and Li,X.
  TITLE     RPA3 promotes the proliferation, migration, and invasion of gliomas
            by activating the PI3K-AKT-mTOR pathway
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 69 (5), 80-86 (2023)
   PUBMED   37571896
  REMARK    GeneRIF: RPA3 promotes the proliferation, migration, and invasion
            of gliomas by activating the PI3K-AKT-mTOR pathway.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Chen,L., Hu,K., Liu,Y., Liu,L., Tang,J. and Qin,X.
  TITLE     Knockdown of replication protein A 3 induces protective autophagy
            and enhances cisplatin sensitivity in lung adenocarcinoma by
            inhibiting AKT/mTOR signaling via binding to cyclin-dependent
            kinases regulatory subunit 2
  JOURNAL   Drug Dev Res 83 (7), 1589-1599 (2022)
   PUBMED   35903032
  REMARK    GeneRIF: Knockdown of replication protein A 3 induces protective
            autophagy and enhances cisplatin sensitivity in lung adenocarcinoma
            by inhibiting AKT/mTOR signaling via binding to cyclin-dependent
            kinases regulatory subunit 2.
REFERENCE   3  (bases 1 to 2020)
  AUTHORS   Kim,M., Park,J., Bouhaddou,M., Kim,K., Rojc,A., Modak,M.,
            Soucheray,M., McGregor,M.J., O'Leary,P., Wolf,D., Stevenson,E.,
            Foo,T.K., Mitchell,D., Herrington,K.A., Munoz,D.P., Tutuncuoglu,B.,
            Chen,K.H., Zheng,F., Kreisberg,J.F., Diolaiti,M.E., Gordan,J.D.,
            Coppe,J.P., Swaney,D.L., Xia,B., van 't Veer,L., Ashworth,A.,
            Ideker,T. and Krogan,N.J.
  TITLE     A protein interaction landscape of breast cancer
  JOURNAL   Science 374 (6563), eabf3066 (2021)
   PUBMED   34591612
REFERENCE   4  (bases 1 to 2020)
  AUTHORS   Sun,D., Zhang,K., Fu,Q., Zhang,H., Liu,S., Wang,H., Xu,Z. and
            Wang,J.
  TITLE     Overexpression of replication protein A3 is associated with
            unfavorable outcome in bladder urothelial carcinoma
  JOURNAL   J Cancer Res Ther 17 (3), 771-776 (2021)
   PUBMED   34269312
  REMARK    GeneRIF: Overexpression of replication protein A3 is associated
            with unfavorable outcome in bladder urothelial carcinoma.
REFERENCE   5  (bases 1 to 2020)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 2020)
  AUTHORS   Keshav,K.F., Chen,C. and Dutta,A.
  TITLE     Rpa4, a homolog of the 34-kilodalton subunit of the replication
            protein A complex
  JOURNAL   Mol Cell Biol 15 (6), 3119-3128 (1995)
   PUBMED   7760808
REFERENCE   7  (bases 1 to 2020)
  AUTHORS   He,Z., Henricksen,L.A., Wold,M.S. and Ingles,C.J.
  TITLE     RPA involvement in the damage-recognition and incision steps of
            nucleotide excision repair
  JOURNAL   Nature 374 (6522), 566-569 (1995)
   PUBMED   7700386
REFERENCE   8  (bases 1 to 2020)
  AUTHORS   Umbricht,C.B., Griffin,C.A., Hawkins,A.L., Grzeschik,K.H.,
            O'Connell,P., Leach,R., Green,E.D. and Kelly,T.J.
  TITLE     High-resolution genomic mapping of the three human replication
            protein A genes (RPA1, RPA2, and RPA3)
  JOURNAL   Genomics 20 (2), 249-257 (1994)
   PUBMED   8020972
REFERENCE   9  (bases 1 to 2020)
  AUTHORS   Umbricht,C.B., Erdile,L.F., Jabs,E.W. and Kelly,T.J.
  TITLE     Cloning, overexpression, and genomic mapping of the 14-kDa subunit
            of human replication protein A
  JOURNAL   J Biol Chem 268 (9), 6131-6138 (1993)
   PUBMED   8454588
REFERENCE   10 (bases 1 to 2020)
  AUTHORS   Kenny,M.K., Schlegel,U., Furneaux,H. and Hurwitz,J.
  TITLE     The role of human single-stranded DNA binding protein and its
            individual subunits in simian virus 40 DNA replication
  JOURNAL   J Biol Chem 265 (13), 7693-7700 (1990)
   PUBMED   2159011
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC009868.2, AC004948.2 and AI243725.1.
            
            On May 17, 2019 this sequence version replaced NM_002947.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC009868.2 [ECO:0000332]
            RNAseq introns              :: partial sample support SAMEA1968540,
                                           SAMEA2142348 [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000223129.8/ ENSP00000223129.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-290               BC009868.2         1-290
            291-291             AC004948.2         69433-69433         c
            292-524             BC009868.2         292-524
            525-525             AC004948.2         24545-24545         c
            526-1593            BC009868.2         526-1593
            1594-1821           AI243725.1         9-236               c
            1822-2020           AC004948.2         19997-20195         c
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p21.3"
     gene            1..2020
                     /gene="RPA3"
                     /gene_synonym="REPA3; RP-A p14"
                     /note="replication protein A3"
                     /db_xref="GeneID:6119"
                     /db_xref="HGNC:HGNC:10291"
                     /db_xref="MIM:179837"
     exon            1..93
                     /gene="RPA3"
                     /gene_synonym="REPA3; RP-A p14"
                     /inference="alignment:Splign:2.1.0"
     exon            94..145
                     /gene="RPA3"
                     /gene_synonym="REPA3; RP-A p14"
                     /inference="alignment:Splign:2.1.0"
     exon            146..246
                     /gene="RPA3"
                     /gene_synonym="REPA3; RP-A p14"
                     /inference="alignment:Splign:2.1.0"
     exon            247..415
                     /gene="RPA3"
                     /gene_synonym="REPA3; RP-A p14"
                     /inference="alignment:Splign:2.1.0"
     exon            416..1271
                     /gene="RPA3"
                     /gene_synonym="REPA3; RP-A p14"
                     /inference="alignment:Splign:2.1.0"
     CDS             1173..1538
                     /gene="RPA3"
                     /gene_synonym="REPA3; RP-A p14"
                     /note="RF-A protein 3; replication factor A protein 3;
                     replication protein A3, 14kDa"
                     /codon_start=1
                     /product="replication protein A 14 kDa subunit"
                     /protein_id="NP_002938.1"
                     /db_xref="CCDS:CCDS5356.1"
                     /db_xref="GeneID:6119"
                     /db_xref="HGNC:HGNC:10291"
                     /db_xref="MIM:179837"
                     /translation="MVDMMDLPRSRINAGMLAQFIDKPVCFVGRLEKIHPTGKMFILS
                     DGEGKNGTIELMEPLDEEISGIVEVVGRVTAKATILCTSYVQFKEDSHPFDLGLYNEA
                     VKIIHDFPQFYPLGIVQHD"
     exon            1272..1346
                     /gene="RPA3"
                     /gene_synonym="REPA3; RP-A p14"
                     /inference="alignment:Splign:2.1.0"
     exon            1347..1455
                     /gene="RPA3"
                     /gene_synonym="REPA3; RP-A p14"
                     /inference="alignment:Splign:2.1.0"
     exon            1456..2020
                     /gene="RPA3"
                     /gene_synonym="REPA3; RP-A p14"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1575..1580
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RPA3"
                     /gene_synonym="REPA3; RP-A p14"
                     /note="hexamer: ATTAAA"
     polyA_site      1615
                     /gene="RPA3"
                     /gene_synonym="REPA3; RP-A p14"
                     /note="major polyA site"
     regulatory      1761..1766
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RPA3"
                     /gene_synonym="REPA3; RP-A p14"
                     /note="hexamer: AATAAA"
     polyA_site      1784
                     /gene="RPA3"
                     /gene_synonym="REPA3; RP-A p14"
                     /note="major polyA site"
     regulatory      1799..1804
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RPA3"
                     /gene_synonym="REPA3; RP-A p14"
                     /note="hexamer: AATAAA"
     polyA_site      1822
                     /gene="RPA3"
                     /gene_synonym="REPA3; RP-A p14"
                     /note="major polyA site"
     regulatory      1913..1918
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RPA3"
                     /gene_synonym="REPA3; RP-A p14"
                     /note="hexamer: AATAAA"
     polyA_site      1939
                     /gene="RPA3"
                     /gene_synonym="REPA3; RP-A p14"
ORIGIN      
        1 aagctactca gataagaggc tccaagagga catttttgga tgtgaaaaac aatgagaagg
       61 aggacaacac acatttacaa tcgtcttaat tttgtactca gaaaaaggat gtgaagacaa
      121 tgcacaggga atacaatagt ttcagatctg tgtacagttt ccttttgctt catctcctgc
      181 aacaatgtaa tgaagacacc atgatatcat taacatttca cacaaaagga aaatgaggct
      241 gaaatggtgt gggcaaggcc caggaatctg gagcatccct aaccaagcag gagagcacct
      301 gggatagaga aagtgctcaa gaatgttcac ttactgatta ctacaatcaa aaaaagatac
      361 gacactaatt taccacattc ttcttactta ttttatgaga tactattctt ccaaggtgga
      421 gaaagtggag aaagtagagt gacgcagcta agggagtaaa tcgaccctca gccaacaagt
      481 ggcaaaagcc tgaagaaagt gatcaagatc actgatgacc ccgctgccca tctccaaggg
      541 ggcgggtatc acaaccccga cgccacacca cgtatcattc cgcaaaactc ccgcgcctcc
      601 cacgcagaac tggcaagagg gaaggcgaga cagcagtgaa cagctggtac gcagcaccca
      661 cagcaccgcg gcagcagcta gtgccgactc ccgcctagct cttttgactc tgttcgcggg
      721 aagaatgggg aaacagtaag gttgcggcgc ctcccgcgag acgaggtacc tgaggctggc
      781 cccgcagtcc cccgccgcac cagcaccgga gcttcacacc ccacttccgg ggtcaagtca
      841 ccgccgggaa tcctgtgatc gcagaaaggt agtctcaggt tccgccccta tccaagtccc
      901 gcctccactg cctctcgccc tgtatctgtc aacttccggg acgccgcgcg tcactaagca
      961 gccaatctcc acttccggac tcatccagcc ccttctccac ccctttcaga gacagcgcga
     1021 ttgcgattta ggtttccgcg catttaattg gcgaagctgg agcgctagtc ttcgctgatt
     1081 ggtgccgaga aatctgcccc atagacaccc gcggggcgca cagtttcagt cgtccgtggg
     1141 tttcccgcca gccgcagtct tggaccataa tcatggtgga catgatggac ttgcccaggt
     1201 cgcgcatcaa cgccggcatg ctagctcaat tcatcgacaa gcctgtctgc ttcgtaggga
     1261 ggctggaaaa gattcatccc accggaaaaa tgtttattct ttcagatgga gaaggaaaaa
     1321 atggaaccat cgagttgatg gaaccccttg atgaagaaat ctctggaatt gtggaagtgg
     1381 ttggaagagt aaccgccaag gccaccatct tgtgtacatc ttatgtccag tttaaagaag
     1441 atagccatcc ttttgatctt ggactttaca atgaagctgt gaaaattatc catgacttcc
     1501 ctcagtttta tcctttaggg attgtgcaac atgattgatc ttgatggatt ttcatacgat
     1561 tgtaaatgag ctatattaaa gtctattaaa ggaagccctt cttgtttgag ggagagattt
     1621 ctgtgctttc tcatatttaa tttgctgttt ttaagatatt ccaacctaga gtttttgatg
     1681 gaactgatat attgacagtt ctcaccgaag tccttttata aagaattgct actccaatat
     1741 atggtcagat tagatgcaag aataaagcag ttgtccgagt ctaagtttct attttattaa
     1801 taaaaactaa aatggtacgt actatcggtc atttcatttt cattctttta atcatgtatt
     1861 caagcacaaa cttgaaattt catagccata aggtcaagat ttagacctac caaataaaac
     1921 cttgggccag ctgtgttaag gatttgctca ccttttccca aactatacct tgataattat
     1981 ttccttgata ccctacttac aaaatgaagt agatgacatt
//
