LOCUS       NM_002946               1584 bp    mRNA    linear   PRI 17-JUN-2024
DEFINITION  Homo sapiens replication protein A2 (RPA2), transcript variant 1,
            mRNA.
ACCESSION   NM_002946
VERSION     NM_002946.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Batenburg,N.L., Sowa,D.J., Walker,J.R., Andres,S.N. and Zhu,X.D.
  TITLE     CSB and SMARCAL1 compete for RPA32 at stalled forks and
            differentially control the fate of stalled forks in BRCA2-deficient
            cells
  JOURNAL   Nucleic Acids Res 52 (9), 5067-5087 (2024)
   PUBMED   38416570
  REMARK    GeneRIF: CSB and SMARCAL1 compete for RPA32 at stalled forks and
            differentially control the fate of stalled forks in BRCA2-deficient
            cells.
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Liang,C.C., Greenhough,L.A., Masino,L., Maslen,S., Bajrami,I.,
            Tuppi,M., Skehel,M., Taylor,I.A. and West,S.C.
  TITLE     Mechanism of single-stranded DNA annealing by RAD52-RPA complex
  JOURNAL   Nature 629 (8012), 697-703 (2024)
   PUBMED   38658755
  REMARK    GeneRIF: Mechanism of single-stranded DNA annealing by RAD52-RPA
            complex.
REFERENCE   3  (bases 1 to 1584)
  AUTHORS   Wang,H., Guan,J., Wang,H., Perrault,A.R., Wang,Y. and Iliakis,G.
  TITLE     Replication protein A2 phosphorylation after DNA damage by the
            coordinated action of ataxia telangiectasia-mutated and
            DNA-dependent protein kinase
  JOURNAL   Cancer Res 61 (23), 8554-8563 (2001)
   PUBMED   11731442
  REMARK    GeneRIF: Phosphorylation of the RPA2 subunit is observed after
            exposure of cells to ionizing radiation (IR) and other DNA-damaging
            agents, which implicates the modified protein in the regulation of
            DNA replication after DNA damage or in DNA repair.
REFERENCE   4  (bases 1 to 1584)
  AUTHORS   Bastin-Shanower,S.A. and Brill,S.J.
  TITLE     Functional analysis of the four DNA binding domains of replication
            protein A. The role of RPA2 in ssDNA binding
  JOURNAL   J Biol Chem 276 (39), 36446-36453 (2001)
   PUBMED   11479296
REFERENCE   5  (bases 1 to 1584)
  AUTHORS   Bochkareva,E., Frappier,L., Edwards,A.M. and Bochkarev,A.
  TITLE     The RPA32 subunit of human replication protein A contains a
            single-stranded DNA-binding domain
  JOURNAL   J Biol Chem 273 (7), 3932-3936 (1998)
   PUBMED   9461578
REFERENCE   6  (bases 1 to 1584)
  AUTHORS   Nagelhus,T.A., Haug,T., Singh,K.K., Keshav,K.F., Skorpen,F.,
            Otterlei,M., Bharati,S., Lindmo,T., Benichou,S., Benarous,R. and
            Krokan,H.E.
  TITLE     A sequence in the N-terminal region of human uracil-DNA glycosylase
            with homology to XPA interacts with the C-terminal part of the
            34-kDa subunit of replication protein A
  JOURNAL   J Biol Chem 272 (10), 6561-6566 (1997)
   PUBMED   9045683
REFERENCE   7  (bases 1 to 1584)
  AUTHORS   Umbricht,C.B., Griffin,C.A., Hawkins,A.L., Grzeschik,K.H.,
            O'Connell,P., Leach,R., Green,E.D. and Kelly,T.J.
  TITLE     High-resolution genomic mapping of the three human replication
            protein A genes (RPA1, RPA2, and RPA3)
  JOURNAL   Genomics 20 (2), 249-257 (1994)
   PUBMED   8020972
REFERENCE   8  (bases 1 to 1584)
  AUTHORS   Dutta,A. and Stillman,B.
  TITLE     cdc2 family kinases phosphorylate a human cell DNA replication
            factor, RPA, and activate DNA replication
  JOURNAL   EMBO J 11 (6), 2189-2199 (1992)
   PUBMED   1318195
REFERENCE   9  (bases 1 to 1584)
  AUTHORS   Din,S., Brill,S.J., Fairman,M.P. and Stillman,B.
  TITLE     Cell-cycle-regulated phosphorylation of DNA replication factor A
            from human and yeast cells
  JOURNAL   Genes Dev 4 (6), 968-977 (1990)
   PUBMED   2200738
REFERENCE   10 (bases 1 to 1584)
  AUTHORS   Erdile,L.F., Wold,M.S. and Kelly,T.J.
  TITLE     The primary structure of the 32-kDa subunit of human replication
            protein A
  JOURNAL   J Biol Chem 265 (6), 3177-3182 (1990)
   PUBMED   2406247
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB025488.1, BC001630.1 and
            BG107669.1.
            
            On Nov 23, 2018 this sequence version replaced NM_002946.4.
            
            Summary: This gene encodes a subunit of the heterotrimeric
            Replication Protein A (RPA) complex, which binds to single-stranded
            DNA (ssDNA), forming a nucleoprotein complex that plays an
            important role in DNA metabolism, being involved in DNA
            replication, repair, recombination, telomere maintenance, and
            co-ordinating the cellular response to DNA damage through
            activation of the ataxia telangiectasia and Rad3-related protein
            (ATR) kinase. The RPA complex protects single-stranded DNA from
            nucleases, prevents formation of secondary structures that would
            interfere with repair, and co-ordinates the recruitment and
            departure of different genome maintenance factors. The
            heterotrimeric complex has two different modes of ssDNA binding, a
            low-affinity and high-affinity mode, determined by which
            oligonucleotide/oligosaccharide-binding (OB) domains of the complex
            are utilized, and differing in the length of DNA bound. This
            subunit contains a single OB domain that participates in
            high-affinity DNA binding and also contains a winged helix domain
            at its carboxy terminus, which interacts with many genome
            maintenance protein. Post-translational modifications of the RPA
            complex also plays a role in co-ordinating different damage
            response pathways. [provided by RefSeq, Sep 2017].
            
            Transcript Variant: This variant (1) encodes isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.28402.1,
                                           SRR3476690.384967.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000373912.8/ ENSP00000363021.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-41                DB025488.1         223-263
            42-1570             BC001630.1         1-1529
            1571-1584           BG107669.1         299-312
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p35.3"
     gene            1..1584
                     /gene="RPA2"
                     /gene_synonym="REPA2; RP-A p32; RP-A p34; RPA32"
                     /note="replication protein A2"
                     /db_xref="GeneID:6118"
                     /db_xref="HGNC:HGNC:10290"
                     /db_xref="MIM:179836"
     exon            1..142
                     /gene="RPA2"
                     /gene_synonym="REPA2; RP-A p32; RP-A p34; RPA32"
                     /inference="alignment:Splign:2.1.0"
     CDS             133..945
                     /gene="RPA2"
                     /gene_synonym="REPA2; RP-A p32; RP-A p34; RPA32"
                     /note="isoform 1 is encoded by transcript variant 1;
                     replication protein A 32 kDa subunit; RF-A protein 2;
                     replication factor A protein 2; replication protein A 34
                     kDa subunit"
                     /codon_start=1
                     /product="replication protein A 32 kDa subunit isoform 1"
                     /protein_id="NP_002937.1"
                     /db_xref="CCDS:CCDS314.1"
                     /db_xref="GeneID:6118"
                     /db_xref="HGNC:HGNC:10290"
                     /db_xref="MIM:179836"
                     /translation="MWNSGFESYGSSSYGGAGGYTQSPGGFGSPAPSQAEKKSRARAQ
                     HIVPCTISQLLSATLVDEVFRIGNVEISQVTIVGIIRHAEKAPTNIVYKIDDMTAAPM
                     DVRQWVDTDDTSSENTVVPPETYVKVAGHLRSFQNKKSLVAFKIMPLEDMNEFTTHIL
                     EVINAHMVLSKANSQPSAGRAPISNPGMSEAGNFGGNSFMPANGLTVAQNQVLNLIKA
                     CPRPEGLNFQDLKNQLKHMSVSSIKQAVDFLSNEGHIYSTVDDDHFKSTDAE"
     exon            143..249
                     /gene="RPA2"
                     /gene_synonym="REPA2; RP-A p32; RP-A p34; RPA32"
                     /inference="alignment:Splign:2.1.0"
     exon            250..351
                     /gene="RPA2"
                     /gene_synonym="REPA2; RP-A p32; RP-A p34; RPA32"
                     /inference="alignment:Splign:2.1.0"
     exon            352..465
                     /gene="RPA2"
                     /gene_synonym="REPA2; RP-A p32; RP-A p34; RPA32"
                     /inference="alignment:Splign:2.1.0"
     exon            466..540
                     /gene="RPA2"
                     /gene_synonym="REPA2; RP-A p32; RP-A p34; RPA32"
                     /inference="alignment:Splign:2.1.0"
     exon            541..657
                     /gene="RPA2"
                     /gene_synonym="REPA2; RP-A p32; RP-A p34; RPA32"
                     /inference="alignment:Splign:2.1.0"
     exon            658..765
                     /gene="RPA2"
                     /gene_synonym="REPA2; RP-A p32; RP-A p34; RPA32"
                     /inference="alignment:Splign:2.1.0"
     exon            766..860
                     /gene="RPA2"
                     /gene_synonym="REPA2; RP-A p32; RP-A p34; RPA32"
                     /inference="alignment:Splign:2.1.0"
     exon            861..1584
                     /gene="RPA2"
                     /gene_synonym="REPA2; RP-A p32; RP-A p34; RPA32"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1551..1556
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RPA2"
                     /gene_synonym="REPA2; RP-A p32; RP-A p34; RPA32"
                     /note="hexamer: AATAAA"
     polyA_site      1584
                     /gene="RPA2"
                     /gene_synonym="REPA2; RP-A p32; RP-A p34; RPA32"
                     /note="major polyA site"
ORIGIN      
        1 attcgcggga aggcgtttgt ggtgccagag aaaagtagcc agagcggcgc agtggcggcc
       61 gcgttctgtg gttttccgct attcccccag acccgcacct tctcggcctc tttgcggaga
      121 atcgtgacca agatgtggaa cagtggattc gaaagctatg gcagctcctc atacggggga
      181 gccggcggct acacgcagtc cccggggggc tttggatcgc ccgcaccttc tcaagccgaa
      241 aagaaatcaa gagcccgagc ccagcacatt gtgccctgta ctatatctca gctgctttct
      301 gccactttgg ttgatgaagt gttcagaatt gggaatgttg agatttcaca ggtcactatt
      361 gtggggatca tcagacatgc agagaaggct ccaaccaaca ttgtttacaa aatagatgac
      421 atgacagctg cacccatgga cgttcgccag tgggttgaca cagatgacac cagcagtgaa
      481 aacactgtgg ttcctccaga aacatatgtg aaagtggcag gccacctgag atcttttcag
      541 aacaaaaaga gcctggtagc ctttaagatc atgcccctgg aggatatgaa tgagttcacc
      601 acacatattc tggaagtgat caatgcacac atggtactaa gcaaagccaa cagccagccc
      661 tcagcaggga gagcacctat cagcaatcca ggaatgagtg aagcagggaa ctttggtggg
      721 aatagcttca tgccagcaaa tggcctcact gtggcccaaa accaggtgtt gaatttgatt
      781 aaggcttgtc caagacctga agggttgaac tttcaggatc tcaagaacca gctgaaacac
      841 atgtctgtat cctcaatcaa gcaagctgtg gattttctga gcaatgaggg gcacatctat
      901 tctactgtgg atgatgacca ttttaaatcc acagatgcag aataactgga tctaactggg
      961 tacctgagat attttacagc tggacctagt ttcacaatct gttgtctcca gctctgcata
     1021 tgtctggcca gggggcttct aggaagtagg tttcatctat caaatgtctc ctctgacttc
     1081 cttttgaaac ttactgctct tctgttttat tttgttttgt ttgaagctca gagggagatg
     1141 ggcaattgac agggatgcaa tccagggtgg gatttcttga ggaagttaca aataagcttg
     1201 ttacaacatc aagatagatg gaattggaag gatgctacca ggagagtact tacatagtgc
     1261 tcaggagttt ctcttcttaa aatgtttact gctgaaagat gagcaggacc agggcgttat
     1321 aggcagagcc ctagccgaga aacctgctgg cctctgcctg ttttcatttc ccactttggt
     1381 tgtgtggcat tactttcaga attgcacttt cctgcttgtc atgacttttt gacacacttg
     1441 ccatgacgtg tgtttctgtg aacatgaagt tctgcggtag tgcctccagg ggcagaggaa
     1501 aagaagaagt gttactgcat tttgtacaaa ataaatacag tcatatgttt aataaaacag
     1561 ttctattgta gtaacttgta aaaa
//
