LOCUS       NM_002945               4847 bp    mRNA    linear   PRI 10-OCT-2024
DEFINITION  Homo sapiens replication protein A1 (RPA1), transcript variant 1,
            mRNA.
ACCESSION   NM_002945 XM_001717905
VERSION     NM_002945.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Pytko,K.G., Dannenberg,R.L., Eckert,K.A. and Hedglin,M.
  TITLE     Replication of [AT/TA]25 Microsatellite Sequences by Human DNA
            Polymerase delta Holoenzymes Is Dependent on dNTP and RPA Levels
  JOURNAL   Biochemistry 63 (8), 969-983 (2024)
   PUBMED   38623046
  REMARK    GeneRIF: Replication of [AT/TA]25 Microsatellite Sequences by Human
            DNA Polymerase delta Holoenzymes Is Dependent on dNTP and RPA
            Levels.
REFERENCE   2  (bases 1 to 4847)
  AUTHORS   Kumar,N., Taneja,A., Ghosh,M., Rothweiler,U., Sundaresan,N.R. and
            Singh,M.
  TITLE     Harmonin homology domain-mediated interaction of RTEL1 helicase
            with RPA and DNA provides insights into its recruitment to DNA
            repair sites
  JOURNAL   Nucleic Acids Res 52 (3), 1450-1470 (2024)
   PUBMED   38153196
  REMARK    GeneRIF: Harmonin homology domain-mediated interaction of RTEL1
            helicase with RPA and DNA provides insights into its recruitment to
            DNA repair sites.
REFERENCE   3  (bases 1 to 4847)
  AUTHORS   Haring,S.J., Mason,A.C., Binz,S.K. and Wold,M.S.
  TITLE     Cellular functions of human RPA1. Multiple roles of domains in
            replication, repair, and checkpoints
  JOURNAL   J Biol Chem 283 (27), 19095-19111 (2008)
   PUBMED   18469000
  REMARK    GeneRIF: as long as a threshold of RPA1-ssDNA binding activity is
            met, DNA replication can occur and that an RPA1 activity separate
            from ssDNA binding is essential for function in DNA repair.
REFERENCE   4  (bases 1 to 4847)
  AUTHORS   Zou,L. and Elledge,S.J.
  TITLE     Sensing DNA damage through ATRIP recognition of RPA-ssDNA complexes
  JOURNAL   Science 300 (5625), 1542-1548 (2003)
   PUBMED   12791985
  REMARK    GeneRIF: data suggest that RPA-coated ssDNA is the critical
            structure at sites of DNA damage that recruits the ATR-ATRIP
            complex and facilitates its recognition of substrates for
            phosphorylation and the initiation of checkpoint signaling
REFERENCE   5  (bases 1 to 4847)
  AUTHORS   Gomes,X.V. and Wold,M.S.
  TITLE     Functional domains of the 70-kilodalton subunit of human
            replication protein A
  JOURNAL   Biochemistry 35 (32), 10558-10568 (1996)
   PUBMED   8756712
REFERENCE   6  (bases 1 to 4847)
  AUTHORS   Umbricht,C.B., Erdile,L.F., Jabs,E.W. and Kelly,T.J.
  TITLE     Cloning, overexpression, and genomic mapping of the 14-kDa subunit
            of human replication protein A
  JOURNAL   J Biol Chem 268 (9), 6131-6138 (1993)
   PUBMED   8454588
REFERENCE   7  (bases 1 to 4847)
  AUTHORS   Savage,S.A. and Niewisch,M.R.
  TITLE     Dyskeratosis Congenita and Related Telomere Biology Disorders
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301779
REFERENCE   8  (bases 1 to 4847)
  AUTHORS   Dornreiter,I., Erdile,L.F., Gilbert,I.U., von Winkler,D.,
            Kelly,T.J. and Fanning,E.
  TITLE     Interaction of DNA polymerase alpha-primase with cellular
            replication protein A and SV40 T antigen
  JOURNAL   EMBO J 11 (2), 769-776 (1992)
   PUBMED   1311258
REFERENCE   9  (bases 1 to 4847)
  AUTHORS   Erdile,L.F., Heyer,W.D., Kolodner,R. and Kelly,T.J.
  TITLE     Characterization of a cDNA encoding the 70-kDa single-stranded
            DNA-binding subunit of human replication protein A and the role of
            the protein in DNA replication
  JOURNAL   J Biol Chem 266 (18), 12090-12098 (1991)
   PUBMED   2050703
  REMARK    Erratum:[J Biol Chem 1993 Jan 25;268(3):2268]
REFERENCE   10 (bases 1 to 4847)
  AUTHORS   Kenny,M.K., Schlegel,U., Furneaux,H. and Hurwitz,J.
  TITLE     The role of human single-stranded DNA binding protein and its
            individual subunits in simian virus 40 DNA replication
  JOURNAL   J Biol Chem 265 (13), 7693-7700 (1990)
   PUBMED   2159011
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AW966691.1, BC018126.1,
            M63488.1, BP432249.1, BM666146.1, R56356.1, AC130689.8, BX110398.1
            and BQ004759.1.
            
            On May 17, 2019 this sequence version replaced NM_002945.4.
            
            Summary: This gene encodes the largest subunit of the
            heterotrimeric Replication Protein A (RPA) complex, which binds to
            single-stranded DNA (ssDNA), forming a nucleoprotein complex that
            plays an important role in DNA metabolism, being involved in DNA
            replication, repair, recombination, telomere maintenance, and
            co-ordinating the cellular response to DNA damage through
            activation of the ataxia telangiectasia and Rad3-related protein
            (ATR) kinase. The nucleoprotein complex protects the
            single-stranded DNA from nucleases, prevents formation of secondary
            structures that would interfere with repair, and co-ordinates the
            recruitment and departure of different genome maintenance factors.
            This subunit contains four oligonucleotide/oligosaccharide-binding
            (OB) domains, though the majority of ssDNA binding occurs in two of
            these domains. The heterotrimeric complex has two different modes
            of ssDNA binding, a low-affinity and high-affinity mode, determined
            by which ssDNA binding domains are utilized. The different binding
            modes differ in the length of DNA bound and in the proteins with
            which it interacts, thereby playing a role in regulating different
            genomic maintenance pathways. [provided by RefSeq, Sep 2017].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.56295.1,
                                           SRR1803615.2999.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000254719.10/ ENSP00000254719.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-46                AW966691.1         34-79
            47-1144             BC018126.1         1-1098
            1145-2067           M63488.1           1125-2047
            2068-2317           BP432249.1         38-287
            2318-2812           BM666146.1         41-535              c
            2813-3142           R56356.1           1-330
            3143-3210           AC130689.8         53546-53613         c
            3211-3888           BX110398.1         8-685
            3889-4319           BQ004759.1         19-449              c
            4320-4847           AC130689.8         51909-52436         c
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17p13.3"
     gene            1..4847
                     /gene="RPA1"
                     /gene_synonym="HSSB; MST075; PFBMFT6; REPA1; RF-A; RP-A;
                     RPA70"
                     /note="replication protein A1"
                     /db_xref="GeneID:6117"
                     /db_xref="HGNC:HGNC:10289"
                     /db_xref="MIM:179835"
     exon            1..122
                     /gene="RPA1"
                     /gene_synonym="HSSB; MST075; PFBMFT6; REPA1; RF-A; RP-A;
                     RPA70"
                     /inference="alignment:Splign:2.1.0"
     CDS             90..1940
                     /gene="RPA1"
                     /gene_synonym="HSSB; MST075; PFBMFT6; REPA1; RF-A; RP-A;
                     RPA70"
                     /note="isoform 1 is encoded by transcript variant 1;
                     MSTP075; RP-A p70; RF-A protein 1; replication factor A
                     protein 1; single-stranded DNA-binding protein;
                     replication protein A1, 70kDa"
                     /codon_start=1
                     /product="replication protein A 70 kDa DNA-binding subunit
                     isoform 1"
                     /protein_id="NP_002936.1"
                     /db_xref="CCDS:CCDS11014.1"
                     /db_xref="GeneID:6117"
                     /db_xref="HGNC:HGNC:10289"
                     /db_xref="MIM:179835"
                     /translation="MVGQLSEGAIAAIMQKGDTNIKPILQVINIRPITTGNSPPRYRL
                     LMSDGLNTLSSFMLATQLNPLVEEEQLSSNCVCQIHRFIVNTLKDGRRVVILMELEVL
                     KSAEAVGVKIGNPVPYNEGLGQPQVAPPAPAASPAASSRPQPQNGSSGMGSTVSKAYG
                     ASKTFGKAAGPSLSHTSGGTQSKVVPIASLTPYQSKWTICARVTNKSQIRTWSNSRGE
                     GKLFSLELVDESGEIRATAFNEQVDKFFPLIEVNKVYYFSKGTLKIANKQFTAVKNDY
                     EMTFNNETSVMPCEDDHHLPTVQFDFTGIDDLENKSKDSLVDIIGICKSYEDATKITV
                     RSNNREVAKRNIYLMDTSGKVVTATLWGEDADKFDGSRQPVLAIKGARVSDFGGRSLS
                     VLSSSTIIANPDIPEAYKLRGWFDAEGQALDGVSISDLKSGGVGGSNTNWKTLYEVKS
                     ENLGQGDKPDYFSSVATVVYLRKENCMYQACPTQDCNKKVIDQQNGLYRCEKCDTEFP
                     NFKYRMILSVNIADFQENQWVTCFQESAEAILGQNAAYLGELKDKNEQAFEEVFQNAN
                     FRSFIFRVRVKVETYNDESRIKATVMDVKPVDYREYGRRLVMSIRRSALM"
     exon            123..173
                     /gene="RPA1"
                     /gene_synonym="HSSB; MST075; PFBMFT6; REPA1; RF-A; RP-A;
                     RPA70"
                     /inference="alignment:Splign:2.1.0"
     exon            174..252
                     /gene="RPA1"
                     /gene_synonym="HSSB; MST075; PFBMFT6; REPA1; RF-A; RP-A;
                     RPA70"
                     /inference="alignment:Splign:2.1.0"
     exon            253..361
                     /gene="RPA1"
                     /gene_synonym="HSSB; MST075; PFBMFT6; REPA1; RF-A; RP-A;
                     RPA70"
                     /inference="alignment:Splign:2.1.0"
     exon            362..450
                     /gene="RPA1"
                     /gene_synonym="HSSB; MST075; PFBMFT6; REPA1; RF-A; RP-A;
                     RPA70"
                     /inference="alignment:Splign:2.1.0"
     exon            451..543
                     /gene="RPA1"
                     /gene_synonym="HSSB; MST075; PFBMFT6; REPA1; RF-A; RP-A;
                     RPA70"
                     /inference="alignment:Splign:2.1.0"
     exon            544..676
                     /gene="RPA1"
                     /gene_synonym="HSSB; MST075; PFBMFT6; REPA1; RF-A; RP-A;
                     RPA70"
                     /inference="alignment:Splign:2.1.0"
     exon            677..779
                     /gene="RPA1"
                     /gene_synonym="HSSB; MST075; PFBMFT6; REPA1; RF-A; RP-A;
                     RPA70"
                     /inference="alignment:Splign:2.1.0"
     exon            780..848
                     /gene="RPA1"
                     /gene_synonym="HSSB; MST075; PFBMFT6; REPA1; RF-A; RP-A;
                     RPA70"
                     /inference="alignment:Splign:2.1.0"
     exon            849..1041
                     /gene="RPA1"
                     /gene_synonym="HSSB; MST075; PFBMFT6; REPA1; RF-A; RP-A;
                     RPA70"
                     /inference="alignment:Splign:2.1.0"
     exon            1042..1181
                     /gene="RPA1"
                     /gene_synonym="HSSB; MST075; PFBMFT6; REPA1; RF-A; RP-A;
                     RPA70"
                     /inference="alignment:Splign:2.1.0"
     exon            1182..1330
                     /gene="RPA1"
                     /gene_synonym="HSSB; MST075; PFBMFT6; REPA1; RF-A; RP-A;
                     RPA70"
                     /inference="alignment:Splign:2.1.0"
     exon            1331..1463
                     /gene="RPA1"
                     /gene_synonym="HSSB; MST075; PFBMFT6; REPA1; RF-A; RP-A;
                     RPA70"
                     /inference="alignment:Splign:2.1.0"
     exon            1464..1640
                     /gene="RPA1"
                     /gene_synonym="HSSB; MST075; PFBMFT6; REPA1; RF-A; RP-A;
                     RPA70"
                     /inference="alignment:Splign:2.1.0"
     exon            1641..1748
                     /gene="RPA1"
                     /gene_synonym="HSSB; MST075; PFBMFT6; REPA1; RF-A; RP-A;
                     RPA70"
                     /inference="alignment:Splign:2.1.0"
     exon            1749..1835
                     /gene="RPA1"
                     /gene_synonym="HSSB; MST075; PFBMFT6; REPA1; RF-A; RP-A;
                     RPA70"
                     /inference="alignment:Splign:2.1.0"
     exon            1836..4847
                     /gene="RPA1"
                     /gene_synonym="HSSB; MST075; PFBMFT6; REPA1; RF-A; RP-A;
                     RPA70"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2809..2814
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RPA1"
                     /gene_synonym="HSSB; MST075; PFBMFT6; REPA1; RF-A; RP-A;
                     RPA70"
                     /note="hexamer: AATAAA"
     polyA_site      2836
                     /gene="RPA1"
                     /gene_synonym="HSSB; MST075; PFBMFT6; REPA1; RF-A; RP-A;
                     RPA70"
                     /note="major polyA site"
     regulatory      4299..4304
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RPA1"
                     /gene_synonym="HSSB; MST075; PFBMFT6; REPA1; RF-A; RP-A;
                     RPA70"
                     /note="hexamer: ATTAAA"
     polyA_site      4319
                     /gene="RPA1"
                     /gene_synonym="HSSB; MST075; PFBMFT6; REPA1; RF-A; RP-A;
                     RPA70"
ORIGIN      
        1 ctcgggccaa taactgcgca gcgcgcggga cccgggtggg gaagctggag ctgttgcggg
       61 gtccgcgggg aagtcttggc ggtggagcca tggtcggcca actgagcgag ggggccattg
      121 cggccatcat gcagaagggg gatacaaaca taaagcccat cctccaagtc atcaacatcc
      181 gtcccattac tacggggaat agtccgccgc gttatcgact gctcatgagt gatggattga
      241 acactctatc ctctttcatg ttggcgacac agttgaaccc tctcgtggag gaagaacaat
      301 tgtccagcaa ctgtgtatgc cagattcaca gatttattgt gaacactctg aaagacggaa
      361 ggagagtagt tatcttgatg gaattagaag ttttgaagtc agctgaagca gttggagtga
      421 agattggcaa tccagtgccc tataatgaag gactcgggca gccgcaagta gctcctccag
      481 cgccagcagc cagcccagca gcaagcagca ggccccagcc gcagaatgga agctcgggaa
      541 tgggttctac tgtttctaag gcttatggtg cttcaaagac atttggaaaa gctgcaggtc
      601 ccagcctgtc acacacttct gggggaacac agtccaaagt ggtgcccatt gccagcctca
      661 ctccttacca gtccaagtgg accatttgtg ctcgtgttac caacaaaagt cagatccgta
      721 cctggagcaa ctcccgaggg gaagggaagc ttttctccct agaactggtt gacgaaagtg
      781 gtgaaatccg agctacagct ttcaatgagc aagtggacaa gttctttcct cttattgaag
      841 tgaacaaggt gtattatttc tcgaaaggca ccctgaagat tgctaacaag cagttcacag
      901 ctgttaaaaa tgactacgag atgaccttca ataacgagac ttccgtcatg ccctgtgagg
      961 acgaccatca tttacctacg gttcagtttg atttcacggg gattgatgac ctcgagaaca
     1021 agtcgaaaga ctcacttgta gacatcatcg ggatctgcaa gagctatgaa gacgccacta
     1081 aaatcacagt gaggtctaac aacagagaag ttgccaagag gaatatctac ttgatggaca
     1141 catccgggaa ggtggtgact gctacactgt ggggggaaga tgctgataaa tttgatggtt
     1201 ctagacagcc cgtgttggct atcaaaggag cccgagtctc tgatttcggt ggacggagcc
     1261 tctccgtgct gtcttcaagc actatcattg cgaatcctga catcccagag gcctataagc
     1321 ttcgtggatg gtttgacgca gaaggacaag ccttagatgg tgtttccatc tctgatctaa
     1381 agagcggcgg agtcggaggg agtaacacca actggaaaac cttgtatgag gtcaaatccg
     1441 agaacctggg ccaaggcgac aagccggact actttagttc tgtggccaca gtggtgtatc
     1501 ttcgcaaaga gaactgcatg taccaagcct gcccgactca ggactgcaat aagaaagtga
     1561 ttgatcaaca gaatggattg taccgctgtg agaagtgcga caccgaattt cccaatttca
     1621 agtaccgcat gatcctgtca gtaaatattg cagattttca agagaatcag tgggtgactt
     1681 gtttccagga gtctgctgaa gctatccttg gacaaaatgc tgcttatctt ggggaattaa
     1741 aagacaagaa tgaacaggca tttgaagaag ttttccagaa tgccaacttc cgatctttca
     1801 tattcagagt cagggtcaaa gtggagacct acaacgacga gtctcgaatt aaggccactg
     1861 tgatggacgt gaagcccgtg gactacagag agtatggccg aaggctggtc atgagcatca
     1921 ggagaagtgc attgatgtga gaggagcagt gccaatcggg cagaagtttg caaataggca
     1981 gaatggaatc gatttcctcc cacctccgtg tgacgatccc atgttagcta cacagtgcag
     2041 aggctcttga tggtggacta agcaatttcc cccctcgtgc gcatctcaga acccatcggt
     2101 aggcaaagga aaatacgctc aggtggttgt ggtgtagact gtgtcaggcc tacggagtca
     2161 gccagtggct agcgcaagac cagtcactcc ctctgccttc aggcttctgt caatttcatt
     2221 atcatcaagc aggaattatg tcgtaagtca ctgaccctaa ctgcagacca tgaagtaaat
     2281 tatgtaacta ggtttttgct tctccagtgg tgaccacccc cccccatccc cgctcacaac
     2341 ttgggttctt ctcagcgggg cgagctgaga agcggtcatg agcacctggg gattttagta
     2401 agtgtgtctt cctagaattc gaaggctctc tctttctaga ggtgctacat agttggtaat
     2461 gcttggaatg gcaatagggt agaatgatta atcaaaggca tatcttctat atctgaagag
     2521 tatccttcct tcagggttta atagactgag tcagatgggt ctgatattaa tcaaaattgt
     2581 ctcttctgag gaccgctgat aagcattgac ttgccgtccc ctaaggaaat ccgagcggct
     2641 acaaagcgtt tctttacttc tcacttcaat taatgctgcg cttcgcttgg tgagtgcgta
     2701 ctttttctac ctgtacacat tcctgcattc atgtattttg ttttttttga ctaaagctat
     2761 gttacatgga aaggattttg aagccttttg tttcccttgc tttgttttaa taaacagtat
     2821 attctttggt tgtgaatcct actttctttg aatgcaaaga gttcctataa ctggaaagca
     2881 attaattagc cttcataata aatgttcact tgggggggat gttaactcat tataaacccg
     2941 aagattagtc caaggcatgg agatccttct tcctagtgtt tgcagccctg aaatgcatct
     3001 ttcaaagcat gaaaacacta aaaacaaaaa gccattttgc ctgaggatgc tgatgatctg
     3061 gcacttggga ttttattgat gtttacgcag cagtctaaca ccaaccacgc tttgaaatgt
     3121 gtacagacag tgagctggta agaaaacagt aattatgcta gtgggccttt cagtcagcaa
     3181 aagcatgctc gctctgtgtg ttcctaatca tattaattat ctatccggtg gctgcaacac
     3241 accgcctgcc attggccgca catctcgccg tcgtaccccg gcagtgcggc ggtcactctg
     3301 cagccagagg acctgctgtt catcactgca catgccgcct gcggaggctt ttggatatgg
     3361 ggagtgatgg tgtcctgtct gtctcccccc tcggtgtctg ccgttgacat aggggccagc
     3421 cagccctaga cgggatgact tccgttcctg aggacagaca cagagggact cctgctcagc
     3481 ctcactaatt gtttagacac attccttcct acccttctct agtctcagga gatggtaact
     3541 gggtcgcatt tcagtctctg actgaggcct cagccatttt tacggaagtt cttttctgtc
     3601 tgaccttgct tataaagcat cgacgagaaa attacagtct tcaaccctct tctggattga
     3661 caaattgtgg ctgggaagtg gcgatctagc tttcagccca gtaaccagtc tttcatgcct
     3721 actactccca gcattccctc ctctccccac gtgtctgtcc acacagtgaa gaggcctgac
     3781 cagccgtggt accaggacag gacgtgtcca gggaacgctg acacctgtcc tcgcgccttc
     3841 tcagtggcca gcgtgatgaa accagcacgt ctccgtggat gtgattggga acccaggggc
     3901 agtgccaggg ggagggctcc ctcgaggagg ctgtttctaa cagatttccc cactcaaaga
     3961 tcagatcacc agcagaggag catcagaaac tggctccact gttgggcttt tcagagattt
     4021 tggtccctgc gggttgccta aatagattct ggcccacagt ttacctcgaa aggctgttga
     4081 tgttgttctg tttctcctct ttcacttaga gatcaatgtt gattttgcgt acaccatgac
     4141 atcagcgtta ggcaattagg agaaaaaaat ctaatcattt cgcctttatt tcaagtggtt
     4201 cttgaattcc ctccagtctc attgtgaaag gggcagggaa aaaaataaaa gggtaataat
     4261 ctgatttctg tccatatttc cagtgtttta tgctttccat taaaaccttg ctaatctctc
     4321 gcgtgctgtt ccttttttga actgcagggt caaaaatgtt cccctccccc acttctgttt
     4381 cctgagtggg ctgtcttcaa aaagtataaa tactattgat cgtggtattt aatacactaa
     4441 tgaaacccat ggttattgac tacataatta atatactgtc caaaagtgaa gtttggccgc
     4501 ccagtatgta aaggtttata ttgactcatg tttctttggc aatatgccgc ccaaggtttt
     4561 atttatagag gatgaaacag gttgtttggt ctccttttct tcttttcacc tgttagaagg
     4621 ccactattcc cctagtaaac tgattgtttc cttgtctcaa agggaagatt ccccccactc
     4681 cccggccacc ttttatgtgg tttgttaact cttagtctta tatccactcc gctggtagag
     4741 ttctggcttt tataaacacc tttggaagtc atcggttcct caaaaagaga gcatagatgt
     4801 ttaattttca cttattcaaa aaaactggta tttttgttat taagaca
//
