LOCUS       NM_001375654            5592 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens RP1 axonemal microtubule associated (RP1), transcript
            variant 2, mRNA.
ACCESSION   NM_001375654
VERSION     NM_001375654.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Natsume,K., Kominami,T., Goto,K., Koyanagi,Y., Inooka,T., Ota,J.,
            Kawano,K., Yamada,K., Okuda,D., Yuki,K., Nishiguchi,K.M. and
            Ushida,H.
  TITLE     Phenotypic variability of RP1-related inherited retinal dystrophy
            associated with the c.5797 C > T (p.Arg1933*) variant in the
            Japanese population
  JOURNAL   Sci Rep 14 (1), 25669 (2024)
   PUBMED   39463394
  REMARK    GeneRIF: Phenotypic variability of RP1-related inherited retinal
            dystrophy associated with the c.5797 C > T (p.Arg1933*) variant in
            the Japanese population.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5592)
  AUTHORS   D'Esposito,F., Randazzo,V., Vega,M.I., Esposito,G., Maltese,P.E.,
            Torregrossa,S., Scibetta,P., Listi,F., Gagliano,C., Scalia,L.,
            Pioppo,A., Marino,A., Piergentili,M., Malvone,E., Fioretti,T.,
            Vitrano,A., Piccione,M., Avitabile,T., Salvatore,F., Bertelli,M.,
            Costagliola,C., Cordeiro,M.F., Maggio,A. and D'Alcamo,E.
  TITLE     RP1 Dominant p.Ser740* Pathogenic Variant in 20 Knowingly Unrelated
            Families Affected by Rod-Cone Dystrophy: Potential Founder Effect
            in Western Sicily
  JOURNAL   Medicina (Kaunas) 60 (2), 254 (2024)
   PUBMED   38399542
  REMARK    GeneRIF: RP1 Dominant p.Ser740* Pathogenic Variant in 20 Knowingly
            Unrelated Families Affected by Rod-Cone Dystrophy: Potential
            Founder Effect in Western Sicily.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5592)
  AUTHORS   Chen,L., Zhao,M., Zhou,M., Luo,J., Li,S., Liu,X., Cheng,Z.,
            Zhuo,Y., Zeng,W., Zhang,Z. and Zhou,L.
  TITLE     LncRNA RP1-276N6.2 Expression and RP1-276N6.2 Gene Polymorphisms
            Contribute to the Risk of Coronary Artery Disease in Chinese Han
            Population
  JOURNAL   DNA Cell Biol 42 (12), 746-752 (2023)
   PUBMED   37843894
  REMARK    GeneRIF: LncRNA RP1-276N6.2 Expression and RP1-276N6.2 Gene
            Polymorphisms Contribute to the Risk of Coronary Artery Disease in
            Chinese Han Population.
REFERENCE   4  (bases 1 to 5592)
  AUTHORS   Lazaro-Guevara,J.M., Flores-Robles,B.J., Garrido-Lopez,K.M.,
            McKeown,R.J., Flores-Moran,A.E., Labrador-Sanchez,E.,
            Pinillos-Aransay,V., Trasahedo,E.A., Lopez-Martin,J.A.,
            Soberanis,L.S.R., Melgar,M.Y., Tellez-Arreola,J.L. and
            Thebault,S.C.
  TITLE     Identification of RP1 as the genetic cause of retinitis pigmentosa
            in a multi-generational pedigree using Extremely Low-Coverage Whole
            Genome Sequencing (XLC-WGS)
  JOURNAL   Gene 851, 146956 (2023)
   PUBMED   36341727
  REMARK    GeneRIF: Identification of RP1 as the genetic cause of retinitis
            pigmentosa in a multi-generational pedigree using Extremely
            Low-Coverage Whole Genome Sequencing (XLC-WGS).
REFERENCE   5  (bases 1 to 5592)
  AUTHORS   Won,D., Hwang,J.Y., Shim,Y., Byeon,S.H., Lee,J., Lee,C.S., Kim,M.,
            Lim,H.T., Choi,J.R., Lee,S.T. and Han,J.
  TITLE     In Silico identification of a common mobile element insertion in
            exon 4 of RP1
  JOURNAL   Sci Rep 11 (1), 13381 (2021)
   PUBMED   34183725
  REMARK    GeneRIF: In Silico identification of a common mobile element
            insertion in exon 4 of RP1.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5592)
  AUTHORS   Sullivan,L.S., Heckenlively,J.R., Bowne,S.J., Zuo,J., Hide,W.A.,
            Gal,A., Denton,M., Inglehearn,C.F., Blanton,S.H. and Daiger,S.P.
  TITLE     Mutations in a novel retina-specific gene cause autosomal dominant
            retinitis pigmentosa
  JOURNAL   Nat Genet 22 (3), 255-259 (1999)
   PUBMED   10391212
REFERENCE   7  (bases 1 to 5592)
  AUTHORS   Pierce,E.A., Quinn,T., Meehan,T., McGee,T.L., Berson,E.L. and
            Dryja,T.P.
  TITLE     Mutations in a gene encoding a new oxygen-regulated photoreceptor
            protein cause dominant retinitis pigmentosa
  JOURNAL   Nat Genet 22 (3), 248-254 (1999)
   PUBMED   10391211
REFERENCE   8  (bases 1 to 5592)
  AUTHORS   Juwana,J.P., Henderikx,P., Mischo,A., Wadle,A., Fadle,N.,
            Gerlach,K., Arends,J.W., Hoogenboom,H., Pfreundschuh,M. and
            Renner,C.
  TITLE     EB/RP gene family encodes tubulin binding proteins
  JOURNAL   Int J Cancer 81 (2), 275-284 (1999)
   PUBMED   10188731
REFERENCE   9  (bases 1 to 5592)
  AUTHORS   Fahim,A.T., Daiger,S.P. and Weleber,R.G.
  TITLE     Nonsyndromic Retinitis Pigmentosa Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301590
REFERENCE   10 (bases 1 to 5592)
  AUTHORS   Blanton,S.H., Heckenlively,J.R., Cottingham,A.W., Friedman,J.,
            Sadler,L.A., Wagner,M., Friedman,L.H. and Daiger,S.P.
  TITLE     Linkage mapping of autosomal dominant retinitis pigmentosa (RP1) to
            the pericentric region of human chromosome 8
  JOURNAL   Genomics 11 (4), 857-869 (1991)
   PUBMED   1783394
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC091076.7, AF128525.5,
            AC090151.10 and AC090137.4.
            
            Summary: This gene encodes a member of the doublecortin family. The
            protein encoded by this gene contains two doublecortin domains,
            which bind microtubules and regulate microtubule polymerization.
            The encoded protein is a photoreceptor microtubule-associated
            protein and is required for correct stacking of outer segment disc.
            This protein and the RP1L1 protein, another retinal-specific
            protein, play essential and synergistic roles in affecting
            photosensitivity and outer segment morphogenesis of rod
            photoreceptors. Because of its response to in vivo retinal oxygen
            levels, this protein was initially named ORP1 (oxygen-regulated
            protein-1). This protein was subsequently designated RP1 (retinitis
            pigmentosa 1) when it was found that mutations in this gene cause
            autosomal dominant retinitis pigmentosa. Mutations in this gene
            also cause autosomal recessive retinitis pigmentosa. Transcript
            variants resulted from an alternative promoter and alternative
            splicings have been found, which overlap the current reference
            sequence and has several exons upstream and downstream of the
            current reference sequence. However, the biological validity and
            full-length nature of some variants cannot be determined at this
            time.[provided by RefSeq, Sep 2010].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA2158569,
                              SAMN03465409 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-136               AC091076.7         32747-32882         c
            137-763             AF128525.5         37586-38212
            764-935             AF128525.5         38748-38919
            936-1099            AF128525.5         65616-65779
            1100-1186           AF128525.5         69411-69497
            1187-1319           AF128525.5         72714-72846
            1320-1471           AF128525.5         80330-80481
            1472-1550           AF128525.5         90481-90559
            1551-1626           AF128525.5         95092-95167
            1627-1703           AF128525.5         96051-96127
            1704-1735           AF128525.5         96227-96258
            1736-1865           AF128525.5         96435-96564
            1866-1969           AC090151.10        91064-91167
            1970-2146           AC090151.10        93083-93259
            2147-2359           AC090151.10        98040-98252
            2360-2537           AC090151.10        111726-111903
            2538-2669           AC090151.10        117942-118073
            2670-2869           AC090151.10        126142-126341
            2870-2956           AC090151.10        130540-130626
            2957-3089           AC090151.10        146400-146532
            3090-3241           AC090151.10        147216-147367
            3242-3396           AC090151.10        150519-150673
            3397-3455           AC090151.10        161338-161396
            3456-3500           AC090151.10        161720-161764
            3501-3664           AC090137.4         12895-13058
            3665-3884           AC090137.4         66798-67017
            3885-4039           AC090137.4         81922-82076
            4040-4118           AC090137.4         86376-86454
            4119-4200           AC090137.4         95183-95264
            4201-5592           AC090137.4         99191-100582
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q11.23-q12.1"
     gene            1..5592
                     /gene="RP1"
                     /gene_synonym="DCDC4A; ORP1"
                     /note="RP1 axonemal microtubule associated"
                     /db_xref="GeneID:6101"
                     /db_xref="HGNC:HGNC:10263"
                     /db_xref="MIM:603937"
     exon            1..136
                     /gene="RP1"
                     /gene_synonym="DCDC4A; ORP1"
                     /inference="alignment:Splign:2.1.0"
     exon            137..763
                     /gene="RP1"
                     /gene_synonym="DCDC4A; ORP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             149..4300
                     /gene="RP1"
                     /gene_synonym="DCDC4A; ORP1"
                     /note="isoform 2 is encoded by transcript variant 2;
                     oxygen-regulated protein 1; retinitis pigmentosa 1
                     protein; retinitis pigmentosa 1 (autosomal dominant);
                     doublecortin domain containing 4A; retinitis pigmentosa
                     RP1 protein"
                     /codon_start=1
                     /product="oxygen-regulated protein 1 isoform 2"
                     /protein_id="NP_001362583.1"
                     /db_xref="GeneID:6101"
                     /db_xref="HGNC:HGNC:10263"
                     /db_xref="MIM:603937"
                     /translation="MSDTPSTGFSIIHPTSSEGQVPPPRHLSLTHPVVAKRISFYKSG
                     DPQFGGVRVVVNPRSFKSFDALLDNLSRKVPLPFGVRNISTPRGRHSITRLEELEDGE
                     SYLCSHGRKVQPVDLDKARRRPRPWLSSRAISAHSPPHPVAVAAPGMPRPPRSLVVFR
                     NGDPKTRRAVLLSRRVTQSFEAFLQHLTEVMQRPVVKLYATDGRRVPSLQAVILSSGA
                     VVAAGREPFKPGNYDIQKYLLPARLPGISQRVYPKGNAKSESRKSSNWKVFIITSDLP
                     DAGTSSQIYIILYGQHRSSAPIYLYGTDGARFQVGHEDIFTITVGDIGALFKIRIGHT
                     NSGSSPSWHCKEIQLHNMNSGKQFYVPVQRWLARDQEDGEICREFPLLSKGQPVLPVT
                     IYEVNVATGELWNAGTVANVYISIHGEKGDTGSRQLFRSKSSFNFLRGQTDTFFLEAV
                     HLGDLCKIVIGHDGLGPGNGWFLDDVVIKDPTTNYEYAFFCHRWLDQGEDDCKIVREL
                     YARDNSIFSARQKLELKRKETWTAESWKFTKGNTLQFYNKLTGGFVRLHPDGTVDAIG
                     EKTDKYGVFDVIFNKRNICIFQSHEMRHLSLALDNGIVTGMVSGEATTELRVLYQPNR
                     CALLESALVPGHTVVFDRHGKIADASSAGYANLSKEFVIFVKGVFLNSAVVLLATSLC
                     QALCLQPDGSCTGVGSQSEKSYWKVHKISSGICMFESVKNAQMYLRIKDGRCDGTGTG
                     DVDCHFKIKKNLKNASISLESTKSPGLFVGLQSDGQAKPMIYTKDENVCFYPQVIQFG
                     RENPMGMSATPSQEEEKIHESKKQKKIPPESEARSPLPSSTAKEIRHFQSSETLLSED
                     EWKVLVLTGNTGTQANVTLWVYGDKGVTGPISLRKDSSEQLFLPGHEDEFQVEIRNTG
                     NIYKIRIGHDGTSEQPEWNLQRVTMQHMKSKKILDFAANVWLSRIQADGDVVCELPVV
                     KGGQAIFPLVRYQVDVYTGQLKHAETESEVFLCLFGERGDSGLRQLYKSNMQVKFQRG
                     QIDKFQVAAVSLGKLQKVLLRCEASDKSQYWYCEQVIVREPGTTSESIFTCQRWLPFM
                     SQGIIHSEIELYLQEMQINHQPKIQEEANDGDWKVTIVTGDLENAGTTATVFLYVYGE
                     TKCSGPIILGSGKHQLFNPNTADIFKINLKDIGEIYKIRIGHDNTGKNPRWYLEEVRL
                     ENIATYELFCLPVDSWIAENENDGDLWKEIPIMGPKKAPLPVVLYEIRIYTGTMTGAE
                     TESNVFINLIGTRGDSGKRRLHQSKNNEIKFQRGQVDIFSIKAVSLGKLKKVLISHDG
                     TGPGNGWFLGSIVVKSEDEDSSEEVLFLCNRWLDEYQDDGKTQRELLAESSPSAVGET
                     GSTIS"
     exon            764..935
                     /gene="RP1"
                     /gene_synonym="DCDC4A; ORP1"
                     /inference="alignment:Splign:2.1.0"
     exon            936..1099
                     /gene="RP1"
                     /gene_synonym="DCDC4A; ORP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1100..1186
                     /gene="RP1"
                     /gene_synonym="DCDC4A; ORP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1187..1319
                     /gene="RP1"
                     /gene_synonym="DCDC4A; ORP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1320..1471
                     /gene="RP1"
                     /gene_synonym="DCDC4A; ORP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1472..1550
                     /gene="RP1"
                     /gene_synonym="DCDC4A; ORP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1551..1626
                     /gene="RP1"
                     /gene_synonym="DCDC4A; ORP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1627..1703
                     /gene="RP1"
                     /gene_synonym="DCDC4A; ORP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1704..1735
                     /gene="RP1"
                     /gene_synonym="DCDC4A; ORP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1736..1865
                     /gene="RP1"
                     /gene_synonym="DCDC4A; ORP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1866..1969
                     /gene="RP1"
                     /gene_synonym="DCDC4A; ORP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1970..2146
                     /gene="RP1"
                     /gene_synonym="DCDC4A; ORP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2147..2359
                     /gene="RP1"
                     /gene_synonym="DCDC4A; ORP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2360..2537
                     /gene="RP1"
                     /gene_synonym="DCDC4A; ORP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2538..2669
                     /gene="RP1"
                     /gene_synonym="DCDC4A; ORP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2670..2869
                     /gene="RP1"
                     /gene_synonym="DCDC4A; ORP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2870..2956
                     /gene="RP1"
                     /gene_synonym="DCDC4A; ORP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2957..3089
                     /gene="RP1"
                     /gene_synonym="DCDC4A; ORP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3090..3241
                     /gene="RP1"
                     /gene_synonym="DCDC4A; ORP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3242..3396
                     /gene="RP1"
                     /gene_synonym="DCDC4A; ORP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3397..3455
                     /gene="RP1"
                     /gene_synonym="DCDC4A; ORP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3456..3500
                     /gene="RP1"
                     /gene_synonym="DCDC4A; ORP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3501..3664
                     /gene="RP1"
                     /gene_synonym="DCDC4A; ORP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3665..3884
                     /gene="RP1"
                     /gene_synonym="DCDC4A; ORP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3885..4039
                     /gene="RP1"
                     /gene_synonym="DCDC4A; ORP1"
                     /inference="alignment:Splign:2.1.0"
     exon            4040..4118
                     /gene="RP1"
                     /gene_synonym="DCDC4A; ORP1"
                     /inference="alignment:Splign:2.1.0"
     exon            4119..4200
                     /gene="RP1"
                     /gene_synonym="DCDC4A; ORP1"
                     /inference="alignment:Splign:2.1.0"
     exon            4201..5592
                     /gene="RP1"
                     /gene_synonym="DCDC4A; ORP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5570..5575
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RP1"
                     /gene_synonym="DCDC4A; ORP1"
                     /note="hexamer: AATAAA"
     polyA_site      5592
                     /gene="RP1"
                     /gene_synonym="DCDC4A; ORP1"
                     /note="major polyA site"
ORIGIN      
        1 actcgtaggc agcatctgag caacaggctc accagacttc agcctcttca ctccgactct
       61 tccacgctcc agctctcttc attaagatat tctagaagca actgaggagt ttctacaaac
      121 attttggaaa ttacaggtct cagccaaaat gagtgatacc ccttctactg gtttttccat
      181 cattcatcct acgtcttctg aaggtcaagt tccaccccct cgccatttga gcctcactca
      241 tcctgttgtg gccaagcgaa tcagtttcta caagagcgga gacccccaat tcggcggggt
      301 cagggtggtg gtcaaccctc gctcctttaa gtcctttgat gctctgctgg ataacttgtc
      361 caggaaggtg cccctccctt ttggagtgag gaacatcagc acccctcggg gcaggcacag
      421 catcacgcgc ctggaggagc tggaggacgg cgagtcctac ctatgttccc acggcaggaa
      481 ggtgcagcct gtagacctgg acaaagcccg tcggcgcccg cggccctggc tcagcagccg
      541 ggccattagc gcgcactcac cgccccaccc cgtagccgtc gctgctcccg gcatgccccg
      601 ccccccacgg agcctagtgg tcttcaggaa tggcgacccg aagacgaggc gtgcggttct
      661 tctgagcagg agggtcaccc agagcttcga ggcatttcta cagcacctga cagaggtcat
      721 gcagcgccct gtggtcaagc tgtacgctac ggacggaagg agggttccca gcctccaggc
      781 agtgatcctg agctctggag ctgtggtggc ggcaggaagg gagccattta aaccaggaaa
      841 ttatgacatc caaaaatact tgcttcctgc tagattacca gggatctctc agcgtgtgta
      901 ccccaaggga aatgcaaagt cagaaagcag aaagagtagt aactggaaag tttttataat
      961 caccagtgac ttgccagatg cagggaccag ctcacagatt tatattattc tgtatggaca
     1021 acatagaagt tcagccccca tatatcttta tggaacagat ggggctcgat ttcaggttgg
     1081 ccatgaagac atatttacta taacagttgg agacattgga gcactcttta agattcgtat
     1141 tggtcatacc aactctggat cctccccttc ctggcactgc aaggagatac agctgcataa
     1201 tatgaattct ggaaaacagt tttatgtacc tgttcaacga tggttggcac gagatcaaga
     1261 ggatggggaa atctgtcgag aatttcctct tttaagtaaa ggacaacctg tccttccagt
     1321 gacaatatat gaagtgaatg tggcaacggg tgagctatgg aatgctggaa cagtagcaaa
     1381 cgtctacatt tctatccatg gggaaaaggg agatacagga tccagacaac tatttagatc
     1441 gaagagctcc ttcaattttt taagaggaca aactgacacc tttttcctgg aggctgtgca
     1501 ccttggagat ttgtgtaaga ttgtgatagg ccatgatgga cttggcccag gaaatggttg
     1561 gtttcttgat gatgttgtta tcaaggatcc cacaacaaat tacgaatatg ccttcttctg
     1621 tcacagatgg ctagatcagg gggaagatga ttgtaaaatt gtcagagaac tgtatgccag
     1681 ggataacagt atcttctctg cgaggcagaa attggaactt aagagaaaag aaacatggac
     1741 tgcagaaagc tggaagttta caaaaggaaa tactcttcag ttctataaca agctgactgg
     1801 agggtttgtc cgtttgcatc cagatggcac agttgacgcc attggagaga agacagacaa
     1861 atatggtgtc tttgatgtta ttttcaacaa aagaaatata tgcattttcc aaagtcatga
     1921 gatgaggcat ctttctcttg ctcttgacaa tggcattgtc actggaatgg ttagtggtga
     1981 agccaccact gagctgcggg tgctttatca gcctaaccgc tgtgcacttc ttgagtcggc
     2041 actggttcct ggtcacacag ttgtttttga tcgtcatggc aaaatagctg atgcatcatc
     2101 agcaggctat gcaaatcttt caaaagaatt tgtgattttt gtcaaggggg tgttcctcaa
     2161 cagtgctgtg gttctgcttg ctacaagcct gtgtcaggcc ctgtgtctcc agcctgatgg
     2221 aagctgcact ggagtgggaa gccagtctga aaaatcctac tggaaagtgc ataaaatcag
     2281 ctctgggatt tgcatgtttg aaagtgtgaa aaatgcacaa atgtatctaa gaatcaagga
     2341 tggccgatgc gatggaacag gtacaggaga tgttgactgt cattttaaaa ttaagaagaa
     2401 cttgaagaat gcatccataa gcctggaatc tacgaagagc ccaggattgt ttgttggact
     2461 tcagtcagat gggcaggcaa aaccaatgat ttataccaag gatgagaatg tttgcttcta
     2521 tccacaagtc atccaatttg gcagagaaaa cccaatggga atgtctgcaa cacccagcca
     2581 agaagaagaa aagatccatg agtcaaaaaa acagaaaaaa atacccccag aatctgaggc
     2641 taggagtccc ttaccttctt caactgcaaa agaaatcaga catttccaaa gtagtgagac
     2701 tcttctgtca gaagatgaat ggaaggtgtt agtgctgaca ggaaatacag gaactcaagc
     2761 caatgtcact ctctgggtgt atggagataa aggagtcact gggccaataa gtcttcgcaa
     2821 ggacagctca gagcagctct tccttccagg acacgaggat gagtttcagg ttgaaataag
     2881 aaacactgga aacatatata agataaggat aggacatgat ggaaccagtg agcaacccga
     2941 gtggaacttg cagagggtga ccatgcaaca tatgaaaagc aagaagatcc tagattttgc
     3001 agcaaacgta tggctgtctc ggattcaggc agatggggat gttgtctgtg agcttcctgt
     3061 agtaaaagga ggacaagcta tttttccatt agtgagatac caggttgacg tctacactgg
     3121 gcagctgaag catgcagaga cggagtctga ggttttcctc tgtctctttg gggagagggg
     3181 agactctggc ctcagacagc tgtacaaatc taacatgcaa gtaaaatttc aaagaggaca
     3241 gattgataaa tttcaagtag cagcagtgtc gcttggaaaa ctgcagaagg tgctgttgcg
     3301 ctgtgaggcc agtgacaaat cacagtactg gtactgtgaa caagtcatag tcagagagcc
     3361 cggcaccaca tccgagtcca tctttacctg tcaaagatgg cttcctttca tgtctcaggg
     3421 cataattcat tcagaaattg aactttatct tcaagaaatg caaataaatc atcaacctaa
     3481 aatacaagag gaagcaaatg atggagactg gaaggtgacc attgtcactg gggatcttga
     3541 aaatgccggc accacggcaa cagtgttcct ttatgtctat ggagaaacaa aatgttcagg
     3601 tcctattatt ttgggatctg ggaaacacca gctgtttaac cctaacactg cagatatatt
     3661 caagattaat ctcaaagata ttggtgaaat ctataaaata cgtattggac atgacaacac
     3721 tggaaagaat cccaggtggt atctggaaga agttagactt gaaaatatag ccacatatga
     3781 gctattttgt ttacctgttg attcctggat agctgagaat gaaaatgatg gcgatctatg
     3841 gaaggaaata cccatcatgg gaccaaagaa agcacctttg ccagtggtgc tttatgagat
     3901 tcgcatatac acaggcacaa tgacaggtgc agaaacagag tctaatgtat ttatcaacct
     3961 catcggtacc agaggtgatt caggaaaacg aagacttcat cagtccaaaa acaatgaaat
     4021 caagtttcag cgtggacagg ttgatatttt ttccattaag gctgtatctc tcggaaaatt
     4081 gaagaaagtt ctgattagtc atgatggaac aggtccaggt aatggatggt ttcttgggag
     4141 tattgttgtt aagtctgaag atgaagacag cagtgaagag gtgctgtttc tatgcaacag
     4201 atggctagat gagtaccaag atgatggcaa gacccagcgg gaattgctcg cagaaagtag
     4261 tcccagtgca gtaggagaga ctgggtctac catctcctga aagggcccac ttgggcctgt
     4321 cgctccaata tgcttcaaaa ggctattgat ctgattgtga tagtgcattt gtcttttgct
     4381 tgggctggag caaacacctg gggagggtca gtttgtgttg gacccactca ttgtttctga
     4441 gaacttccaa aattgggagg atgcactgac agcctccgcc aacagctgac caagatcagc
     4501 ccctggtatc tggggtaccc atactgctgc tgctcacctc ctccccctcc tcttccttca
     4561 ccttttcttt cccttcctcc ccactcttcc cctccctcct ctcccttctt tcttttcctt
     4621 tttctttcag aaaatctgac ttactgctaa ggttactgaa atgtctaaat aagtattctt
     4681 tgtagaaaat aactgaagag gtacctaata acatttgatg catgaaaaac acttttatgc
     4741 aacctatgtt aattttaaag tggaagtttt attgttagac aatggtatgt ctctctcaga
     4801 attagtggta aaatcaataa gaaaatgtag aaagaaccca accaattggg ttactgtgtg
     4861 ttcccagtgc acgtcctgtg ggtccacttg cagtttgagc ttcgagctgg tgacagagtc
     4921 ctgcaggcag agagcagcag agcgcagggc agatatctca catccatcgg tcactttttc
     4981 tcatgaagcc tcaaatactt gtcaacttgg tgccacaggt gccttcttct aatccgttaa
     5041 tttggcacat aagctaaaac tcatttgcca gccccggaga ggtcaggatg tagcttttga
     5101 tatcaaagac acctgtcttt gaatctctat tttgccccgt gaggtagatg ctatcatcac
     5161 ccacattttg cagatgagga aaccgacaca tggaaaagcc aagtgacctg cccaagctta
     5221 gagagtggaa caagcaggag agccgggatg tgaccctaga agtctgactt cagagcccgt
     5281 gcgcgtccac actgtgacct tggccctcct gccttccctg cttgttttta gtatcccatg
     5341 aaatcaggca gaggggccaa ggaggcaaat cttgcatacc accttcatga tttttgtcgt
     5401 ctgcaatagt acctgtacta gtatatgctt gatattttct tccttgaact gtgtctaaat
     5461 cagtgtattt agaggtgaag agagcttggc ctcatcctaa atcatattcc aaatatccat
     5521 gaaatcactg atttaatatg tcattacatt ttttcctaaa acacattaaa ataaatctat
     5581 gtctaacaaa aa
//
