LOCUS       NM_001317774             994 bp    mRNA    linear   PRI 03-APR-2024
DEFINITION  Homo sapiens rhophilin associated tail protein 1 (ROPN1),
            transcript variant 2, mRNA.
ACCESSION   NM_001317774 XM_005247549
VERSION     NM_001317774.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Liu,Q., Huang,X., Li,Q., He,L., Li,S., Chen,X., Ouyang,Y., Wang,X.
            and Lin,C.
  TITLE     Rhophilin-associated tail protein 1 promotes migration and
            metastasis in triple negative breast cancer via activation of RhoA
  JOURNAL   FASEB J 34 (8), 9959-9971 (2020)
   PUBMED   32427399
  REMARK    GeneRIF: Rhophilin-associated tail protein 1 promotes migration and
            metastasis in triple negative breast cancer via activation of RhoA.
REFERENCE   2  (bases 1 to 994)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 994)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 994)
  AUTHORS   Chen,S., Fragoza,R., Klei,L., Liu,Y., Wang,J., Roeder,K., Devlin,B.
            and Yu,H.
  TITLE     An interactome perturbation framework prioritizes damaging missense
            mutations for developmental disorders
  JOURNAL   Nat Genet 50 (7), 1032-1040 (2018)
   PUBMED   29892012
REFERENCE   5  (bases 1 to 994)
  AUTHORS   Pelloni,M., Paoli,D., Majoli,M., Pallotti,F., Carlini,T., Lenzi,A.
            and Lombardo,F.
  TITLE     Molecular study of human sperm RNA: Ropporin and CABYR in
            asthenozoospermia
  JOURNAL   J Endocrinol Invest 41 (7), 781-787 (2018)
   PUBMED   29247344
  REMARK    GeneRIF: There is downregulation of both ROPN1 and CABYR in
            asthenozoospermic samples and importantly, a positive correlation
            between the expression of the two genes, suggesting that ROPN1 and
            CABYR co-expression is a prerequisite for normal flagellar function
            and sperm motility.
REFERENCE   6  (bases 1 to 994)
  AUTHORS   Newell,A.E., Fiedler,S.E., Ruan,J.M., Pan,J., Wang,P.J.,
            Deininger,J., Corless,C.L. and Carr,D.W.
  TITLE     Protein kinase A RII-like (R2D2) proteins exhibit differential
            localization and AKAP interaction
  JOURNAL   Cell Motil Cytoskeleton 65 (7), 539-552 (2008)
   PUBMED   18421703
REFERENCE   7  (bases 1 to 994)
  AUTHORS   Li,Z., Li,W., Meklat,F., Wang,Z., Zhang,J., Zhang,Y. and Lim,S.H.
  TITLE     A yeast two-hybrid system using Sp17 identified Ropporin as a novel
            cancer-testis antigen in hematologic malignancies
  JOURNAL   Int J Cancer 121 (7), 1507-1511 (2007)
   PUBMED   17551920
  REMARK    GeneRIF: Ropporin gene expression in tumor cells is associated with
            the presence of high titer IgG antibodies against Ropporin,
            suggesting the in vivo translation of the mRNA into protein and the
            immunogenicity of the protein to the autologous hosts.
REFERENCE   8  (bases 1 to 994)
  AUTHORS   Carr,D.W., Fujita,A., Stentz,C.L., Liberty,G.A., Olson,G.E. and
            Narumiya,S.
  TITLE     Identification of sperm-specific proteins that interact with
            A-kinase anchoring proteins in a manner similar to the type II
            regulatory subunit of PKA
  JOURNAL   J Biol Chem 276 (20), 17332-17338 (2001)
   PUBMED   11278869
REFERENCE   9  (bases 1 to 994)
  AUTHORS   Harrington,J.J., Sherf,B., Rundlett,S., Jackson,P.D., Perry,R.,
            Cain,S., Leventhal,C., Thornton,M., Ramachandran,R.,
            Whittington,J., Lerner,L., Costanzo,D., McElligott,K., Boozer,S.,
            Mays,R., Smith,E., Veloso,N., Klika,A., Hess,J., Cothren,K., Lo,K.,
            Offenbacher,J., Danzig,J. and Ducar,M.
  TITLE     Creation of genome-wide protein expression libraries using random
            activation of gene expression
  JOURNAL   Nat Biotechnol 19 (5), 440-445 (2001)
   PUBMED   11329013
REFERENCE   10 (bases 1 to 994)
  AUTHORS   Fujita,A., Nakamura,K., Kato,T., Watanabe,N., Ishizaki,T.,
            Kimura,K., Mizoguchi,A. and Narumiya,S.
  TITLE     Ropporin, a sperm-specific binding protein of rhophilin, that is
            localized in the fibrous sheath of sperm flagella
  JOURNAL   J Cell Sci 113 (Pt 1), 103-112 (2000)
   PUBMED   10591629
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC117381.5.
            
            On Apr 8, 2021 this sequence version replaced NM_001317774.1.
            
            Summary: The protein encoded by this gene is found in the fibrous
            sheath of spermatazoa, where it interacts with rhophilin, a Rho
            GTPase binding protein. The encoded protein also can bind an
            A-kinase anchoring protein (AKAP110) and a calcium-binding tyrosine
            phosphorylation-regulated protein (CABYR). This protein may be
            involved in sperm motility and has been shown to be a cancer-testis
            antigen in hematologic malignancies. Several transcript variants,
            some protein-coding and some non-protein coding, have been found
            for this gene. [provided by RefSeq, Dec 2015].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189667.369475.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1968540,
                                           SAMEA1968832 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000405845.8/ ENSP00000385919.3
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-203               AC117381.5         160855-161057       c
            204-331             AC117381.5         149299-149426       c
            332-449             AC117381.5         145797-145914       c
            450-611             AC117381.5         144312-144473       c
            612-787             AC117381.5         138975-139150       c
            788-994             AC117381.5         137948-138154       c
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q21.1"
     gene            1..994
                     /gene="ROPN1"
                     /gene_synonym="CT91; ODF6; RHPNAP1; ROPN1A; ropporin"
                     /note="rhophilin associated tail protein 1"
                     /db_xref="GeneID:54763"
                     /db_xref="HGNC:HGNC:17692"
                     /db_xref="MIM:611757"
     exon            1..203
                     /gene="ROPN1"
                     /gene_synonym="CT91; ODF6; RHPNAP1; ROPN1A; ropporin"
                     /inference="alignment:Splign:2.1.0"
     exon            204..331
                     /gene="ROPN1"
                     /gene_synonym="CT91; ODF6; RHPNAP1; ROPN1A; ropporin"
                     /inference="alignment:Splign:2.1.0"
     CDS             216..854
                     /gene="ROPN1"
                     /gene_synonym="CT91; ODF6; RHPNAP1; ROPN1A; ropporin"
                     /note="isoform a is encoded by transcript variant 2;
                     ropporin, rhophilin associated protein 1; cancer/testis
                     antigen 91; outer dense fiber of sperm tails 6;
                     ropporin-1A; rhophilin-associated protein 1A; testis
                     secretory sperm-binding protein Li 239w"
                     /codon_start=1
                     /product="ropporin-1A isoform a"
                     /protein_id="NP_001304703.1"
                     /db_xref="CCDS:CCDS3026.1"
                     /db_xref="GeneID:54763"
                     /db_xref="HGNC:HGNC:17692"
                     /db_xref="MIM:611757"
                     /translation="MAQTDKPTCIPPELPKMLKEFAKAAIRVQPQDLIQWAADYFEAL
                     SRGETPPVRERSERVALCNRAELTPELLKILHSQVAGRLIIRAEELAQMWKVVNLPTD
                     LFNSVMNVGRFTEEIEWLKFLALACSALGVTITKTLKIVCEVLSCDHNGGSPRIPFST
                     FQFLYTYIAKVDGEISASHVSRMLNYMEQEVIGPDGIITVNDFTQNPRVQLE"
     exon            332..449
                     /gene="ROPN1"
                     /gene_synonym="CT91; ODF6; RHPNAP1; ROPN1A; ropporin"
                     /inference="alignment:Splign:2.1.0"
     exon            450..611
                     /gene="ROPN1"
                     /gene_synonym="CT91; ODF6; RHPNAP1; ROPN1A; ropporin"
                     /inference="alignment:Splign:2.1.0"
     exon            612..787
                     /gene="ROPN1"
                     /gene_synonym="CT91; ODF6; RHPNAP1; ROPN1A; ropporin"
                     /inference="alignment:Splign:2.1.0"
     exon            788..994
                     /gene="ROPN1"
                     /gene_synonym="CT91; ODF6; RHPNAP1; ROPN1A; ropporin"
                     /inference="alignment:Splign:2.1.0"
     regulatory      965..970
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ROPN1"
                     /gene_synonym="CT91; ODF6; RHPNAP1; ROPN1A; ropporin"
                     /note="hexamer: AATAAA"
     regulatory      973..978
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ROPN1"
                     /gene_synonym="CT91; ODF6; RHPNAP1; ROPN1A; ropporin"
                     /note="hexamer: ATTAAA"
     polyA_site      985
                     /gene="ROPN1"
                     /gene_synonym="CT91; ODF6; RHPNAP1; ROPN1A; ropporin"
                     /note="major polyA site"
     polyA_site      994
                     /gene="ROPN1"
                     /gene_synonym="CT91; ODF6; RHPNAP1; ROPN1A; ropporin"
                     /note="major polyA site"
ORIGIN      
        1 ggagcgctaa cgtctaacgc taacggcggt cgtgccccgc cgctgctgtc acccccggcc
       61 gctgctgccc tccccgccga ggttctactg ctctccttct taagaagggt gggaggcact
      121 cggtctctcc ccacacctct cgcctgaggc caggcgccag gtgtcgcctg aagccagaca
      181 gccggtttgg gagcgagcct gaggccaacc aatcaatggc tcagacagat aagccaacat
      241 gcatcccgcc ggagctgccg aagatgctga aggagtttgc caaagccgcc attagggtgc
      301 agccgcagga cctcatccag tgggcagccg attattttga ggccctgtcc cgtggagaga
      361 cgcctccggt gagagagcgg tctgagcgag tcgctttgtg taaccgggca gagctaacac
      421 ctgagctgtt aaagatcctg cattctcagg ttgctggcag actgatcatc cgtgcagagg
      481 agctggccca gatgtggaaa gtggtgaatc tcccaacaga tctgtttaat agtgtgatga
      541 atgtgggtcg cttcacggag gagatcgagt ggctgaagtt tttagccctt gcttgcagcg
      601 ctctgggagt tactattacc aaaactctca agatagtgtg tgaggtctta tcatgtgacc
      661 ataatggtgg gtcgccccgg atcccgttca gcaccttcca gtttctctac acgtatattg
      721 ccaaagtgga tggggagatc tctgcatcac atgtcagcag gatgctaaac tacatggaac
      781 aggaagtaat tggccctgat ggtataatca cagtgaatga ctttacccaa aaccccaggg
      841 ttcagctgga gtaaaagcac aattttggca attttaaagg aagatacaga gatgattgta
      901 cttcagaatg actgaaaccc atataccacc caaaatcaat tttcttgtac aactggtaca
      961 cactaataaa caattaaaca tatgagatca gaaa
//
