LOCUS       NM_004850               8345 bp    mRNA    linear   PRI 18-NOV-2024
DEFINITION  Homo sapiens Rho associated coiled-coil containing protein kinase 2
            (ROCK2), transcript variant 1, mRNA.
ACCESSION   NM_004850
VERSION     NM_004850.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8345)
  AUTHORS   Chen,X., Fansler,M.M., Janjos,U., Ule,J. and Mayr,C.
  TITLE     The FXR1 network acts as a signaling scaffold for actomyosin
            remodeling
  JOURNAL   Cell 187 (18), 5048-5063 (2024)
   PUBMED   39106863
REFERENCE   2  (bases 1 to 8345)
  AUTHORS   Li,X.X., Xu,J.K., Su,W.J., Wu,H.L., Zhao,K., Zhang,C.M., Chen,X.K.
            and Yang,L.X.
  TITLE     The role of KDM4A-mediated histone methylation on temozolomide
            resistance in glioma cells through the HUWE1/ROCK2 axis
  JOURNAL   Kaohsiung J Med Sci 40 (2), 161-174 (2024)
   PUBMED   37873881
  REMARK    GeneRIF: The role of KDM4A-mediated histone methylation on
            temozolomide resistance in glioma cells through the HUWE1/ROCK2
            axis.
REFERENCE   3  (bases 1 to 8345)
  AUTHORS   Maas-Bauer,K., Stell,A.V., Yan,K.L., de Vega,E., Vinnakota,J.M.,
            Unger,S., Nunez,N., Norona,J., Talvard-Balland,N., Kossmann,S.,
            Schwan,C., Miething,C., Martens,U.S., Shoumariyeh,K., Nestor,R.P.,
            Duquesne,S., Hanke,K., Rackiewicz,M., Hu,Z., El Khawanky,N.,
            Taromi,S., Andrlova,H., Faraidun,H., Walter,S., Pfeifer,D.,
            Follo,M., Waldschmidt,J., Melchinger,W., Rassner,M., Wehr,C.,
            Schmitt-Graeff,A., Halbach,S., Liao,J., Hacker,G., Brummer,T.,
            Dengjel,J., Andrieux,G., Grosse,R., Tugues,S., Blazar,B.R.,
            Becher,B., Boerries,M. and Zeiser,R.
  TITLE     ROCK1/2 signaling contributes to corticosteroid-refractory acute
            graft-versus-host disease
  JOURNAL   Nat Commun 15 (1), 446 (2024)
   PUBMED   38199985
  REMARK    GeneRIF: ROCK1/2 signaling contributes to corticosteroid-refractory
            acute graft-versus-host disease.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8345)
  AUTHORS   Yuan,M., Jing,G., Kong,Q., Ming,T., Zuo,J., Wang,Q., Feng,Y.,
            Liu,W., Wu,X. and Xia,Z.
  TITLE     TIPE2 ameliorates neuroinflammation and cognitive impairment in
            sepsis-associated encephalopathy through regulating
            RhoA/ROCK2-NF-kappaB signaling pathway
  JOURNAL   Biochem Pharmacol 217, 115816 (2023)
   PUBMED   37748665
  REMARK    GeneRIF: TIPE2 ameliorates neuroinflammation and cognitive
            impairment in sepsis-associated encephalopathy through regulating
            RhoA/ROCK2-NF-kappaB signaling pathway.
REFERENCE   5  (bases 1 to 8345)
  AUTHORS   Tan,D., Lu,M., Cai,Y., Qi,W., Wu,F., Bao,H., Qv,M., He,Q., Xu,Y.,
            Wang,X., Shen,T., Luo,J., He,Y., Wu,J., Tang,L., Barkat,M.Q., Xu,C.
            and Wu,X.
  TITLE     SUMOylation of Rho-associated protein kinase 2 induces goblet cell
            metaplasia in allergic airways
  JOURNAL   Nat Commun 14 (1), 3887 (2023)
   PUBMED   37393345
  REMARK    GeneRIF: SUMOylation of Rho-associated protein kinase 2 induces
            goblet cell metaplasia in allergic airways.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8345)
  AUTHORS   Feng,J., Ito,M., Ichikawa,K., Isaka,N., Nishikawa,M.,
            Hartshorne,D.J. and Nakano,T.
  TITLE     Inhibitory phosphorylation site for Rho-associated kinase on smooth
            muscle myosin phosphatase
  JOURNAL   J Biol Chem 274 (52), 37385-37390 (1999)
   PUBMED   10601309
REFERENCE   7  (bases 1 to 8345)
  AUTHORS   Fukata,Y., Oshiro,N., Kinoshita,N., Kawano,Y., Matsuoka,Y.,
            Bennett,V., Matsuura,Y. and Kaibuchi,K.
  TITLE     Phosphorylation of adducin by Rho-kinase plays a crucial role in
            cell motility
  JOURNAL   J Cell Biol 145 (2), 347-361 (1999)
   PUBMED   10209029
REFERENCE   8  (bases 1 to 8345)
  AUTHORS   Takahashi,N., Tuiki,H., Saya,H. and Kaibuchi,K.
  TITLE     Localization of the gene coding for ROCK II/Rho kinase on human
            chromosome 2p24
  JOURNAL   Genomics 55 (2), 235-237 (1999)
   PUBMED   9933571
REFERENCE   9  (bases 1 to 8345)
  AUTHORS   Witke,W., Podtelejnikov,A.V., Di Nardo,A., Sutherland,J.D.,
            Gurniak,C.B., Dotti,C. and Mann,M.
  TITLE     In mouse brain profilin I and profilin II associate with regulators
            of the endocytic pathway and actin assembly
  JOURNAL   EMBO J 17 (4), 967-976 (1998)
   PUBMED   9463375
REFERENCE   10 (bases 1 to 8345)
  AUTHORS   Ishizaki,T., Maekawa,M., Fujisawa,K., Okawa,K., Iwamatsu,A.,
            Fujita,A., Watanabe,N., Saito,Y., Kakizuka,A., Morii,N. and
            Narumiya,S.
  TITLE     The small GTP-binding protein Rho binds to and activates a 160 kDa
            Ser/Thr protein kinase homologous to myotonic dystrophy kinase
  JOURNAL   EMBO J 15 (8), 1885-1893 (1996)
   PUBMED   8617235
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC099344.4, AB014519.1,
            AC018463.11, BC111801.1 and AL049383.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_004850.4.
            
            Summary: The protein encoded by this gene is a serine/threonine
            kinase that regulates cytokinesis, smooth muscle contraction, the
            formation of actin stress fibers and focal adhesions, and the
            activation of the c-fos serum response element. This protein, which
            is an isozyme of ROCK1 is a target for the small GTPase Rho.
            [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB014519.1, D87931.1 [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000315872.11/ ENSP00000317985.6
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-51                AC099344.4         11672-11722         c
            52-300              AB014519.1         1-249
            301-1791            AB014519.1         255-1745
            1792-1820           AC018463.11        68047-68075         c
            1821-4390           AB014519.1         1775-4344
            4391-4449           BC111801.1         2447-2505
            4450-4953           AB014519.1         4404-4907
            4954-7353           AC018463.11        29832-32231         c
            7354-8345           AL049383.1         1986-2977
FEATURES             Location/Qualifiers
     source          1..8345
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p25.1"
     gene            1..8345
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
                     /note="Rho associated coiled-coil containing protein
                     kinase 2"
                     /db_xref="GeneID:9475"
                     /db_xref="HGNC:HGNC:10252"
                     /db_xref="MIM:604002"
     exon            1..641
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
                     /inference="alignment:Splign:2.1.0"
     CDS             501..4667
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1; p164
                     ROCK-2; rho-associated, coiled-coil-containing protein
                     kinase II"
                     /codon_start=1
                     /product="rho-associated protein kinase 2 isoform 1"
                     /protein_id="NP_004841.2"
                     /db_xref="CCDS:CCDS42654.1"
                     /db_xref="GeneID:9475"
                     /db_xref="HGNC:HGNC:10252"
                     /db_xref="MIM:604002"
                     /translation="MSRPPPTGKMPGAPETAPGDGAGASRQRKLEALIRDPRSPINVE
                     SLLDGLNSLVLDLDFPALRKNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGA
                     FGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQ
                     DDRYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPD
                     NMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGFYGRECD
                     WWSVGVFLYEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLT
                     DREVRLGRNGVEEIRQHPFFKNDQWHWDNIRETAAPVVPELSSDIDSSNFDDIEDDKG
                     DVETFPIPKAFVGNQLPFIGFTYYRENLLLSDSPSCRETDSIQSRKNEESQEIQKKLY
                     TLEEHLSNEMQAKEELEQKCKSVNTRLEKTAKELEEEITLRKSVESALRQLEREKALL
                     QHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQNSQISTEKVNQLQRQLDE
                     TNALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQ
                     SALESERRDRTHGSEIINDLQGRICGLEEDLKNGKILLAKVELEKRQLQERFTDLEKE
                     KSNMEIDMTYQLKVIQQSLEQEEAEHKATKARLADKNKIYESIEEAKSEAMKEMEKKL
                     LEERTLKQKVENLLLEAEKRCSLLDCDLKQSQQKINELLKQKDVLNEDVRNLTLKIEQ
                     ETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLMEMKMNLEKQNAELRKERQDAD
                     GQMKELQDQLEAEQYFSTLYKTQVRELKEECEEKTKLGKELQQKKQELQDERDSLAAQ
                     LEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDATIASLE
                     ETNRTLTSDVANLANEKEELNNKLKDVQEQLSRLKDEEISAAAIKAQFEKQLLTERTL
                     KTQAVNKLAEIMNRKEPVKRGNDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKEL
                     NEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQALHIGLDSSSIGSGPGDAEADD
                     GFPESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDK
                     LFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEQEFPVEPVGEKSNYICHKGHE
                     FIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYD
                     ISTAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRP
                     SRQLAPNKPS"
     exon            642..723
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
                     /inference="alignment:Splign:2.1.0"
     exon            724..824
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
                     /inference="alignment:Splign:2.1.0"
     exon            825..962
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
                     /inference="alignment:Splign:2.1.0"
     exon            963..1223
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
                     /inference="alignment:Splign:2.1.0"
     exon            1224..1368
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
                     /inference="alignment:Splign:2.1.0"
     exon            1369..1507
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
                     /inference="alignment:Splign:2.1.0"
     exon            1508..1599
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
                     /inference="alignment:Splign:2.1.0"
     exon            1600..1759
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
                     /inference="alignment:Splign:2.1.0"
     exon            1760..1820
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
                     /inference="alignment:Splign:2.1.0"
     exon            1821..1832
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
                     /inference="alignment:Splign:2.1.0"
     exon            1833..1912
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
                     /inference="alignment:Splign:2.1.0"
     exon            1913..1961
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
                     /inference="alignment:Splign:2.1.0"
     exon            1962..2097
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
                     /inference="alignment:Splign:2.1.0"
     exon            2098..2189
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
                     /inference="alignment:Splign:2.1.0"
     exon            2190..2436
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
                     /inference="alignment:Splign:2.1.0"
     exon            2437..2543
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
                     /inference="alignment:Splign:2.1.0"
     exon            2544..2703
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
                     /inference="alignment:Splign:2.1.0"
     exon            2704..2864
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
                     /inference="alignment:Splign:2.1.0"
     exon            2865..3049
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
                     /inference="alignment:Splign:2.1.0"
     exon            3050..3119
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
                     /inference="alignment:Splign:2.1.0"
     exon            3120..3223
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
                     /inference="alignment:Splign:2.1.0"
     exon            3224..3410
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
                     /inference="alignment:Splign:2.1.0"
     exon            3411..3504
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
                     /inference="alignment:Splign:2.1.0"
     exon            3505..3599
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
                     /inference="alignment:Splign:2.1.0"
     exon            3600..3779
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
                     /inference="alignment:Splign:2.1.0"
     exon            3780..3948
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
                     /inference="alignment:Splign:2.1.0"
     exon            3949..4019
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
                     /inference="alignment:Splign:2.1.0"
     exon            4020..4108
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
                     /inference="alignment:Splign:2.1.0"
     exon            4109..4187
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
                     /inference="alignment:Splign:2.1.0"
     exon            4188..4449
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
                     /inference="alignment:Splign:2.1.0"
     exon            4450..4663
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
                     /inference="alignment:Splign:2.1.0"
     exon            4664..8345
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7315..7320
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
                     /note="hexamer: ATTAAA"
     polyA_site      7335
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
                     /note="major polyA site"
     regulatory      8310..8315
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
                     /note="hexamer: AATAAA"
     regulatory      8320..8325
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
                     /note="hexamer: AATAAA"
     polyA_site      8329
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
     polyA_site      8345
                     /gene="ROCK2"
                     /gene_synonym="ROCK-II"
ORIGIN      
        1 agacgggcgg ccgcgcggga gtgtgtgcgc cgggagctgg ctgagggact gcaaggcggc
       61 cggcggcgac catggcagcg ggccggcggc ggccgtagtg gcccaggcct gggcttcagc
      121 ctcccggggc cccagagggc ggggcggtcc gggccgcggc ggtggcggcg ccacttccct
      181 gctcccgccc gaggactcct gcgggcactc gctgaggacc agcggaccgg cggcgcgaat
      241 ctgactgagg ggcggggacg ccgtctgttc cccgccgctc ccggcagggc cgggccgggc
      301 tgggccgggc tgggccgggc gggcccctgg gagcagcccc caggcggggg accgccttgg
      361 agacccgaag ccggagctag aggcaggcgg tgggcccggg tggagtcccg gccggagctg
      421 gtggttcggg ggcggtgcta ggccccgagg ctgcgggacc tgagcgcgag gagcctgagt
      481 gcgggtccag cggtggcggc atgagccggc ccccgccgac ggggaaaatg cccggcgccc
      541 ccgagaccgc gccgggggac ggggcaggcg cgagccgcca gaggaagctg gaggcgctga
      601 tccgagaccc tcgctccccc atcaacgtgg agagcttgct ggatggctta aattccttgg
      661 tccttgattt agattttcct gctttgagga aaaacaagaa catagataat ttcttaaata
      721 gatatgagaa aattgtgaaa aaaatcagag gtctacagat gaaggcagaa gactatgatg
      781 ttgtaaaagt tattggaaga ggtgcttttg gtgaagtgca gttggttcgt cacaaggcat
      841 cgcagaaggt ttatgctatg aagcttctta gtaagtttga aatgataaaa agatcagatt
      901 ctgccttttt ttgggaagaa agagatatta tggcctttgc caatagcccc tgggtggttc
      961 agctttttta tgcctttcaa gatgataggt atctgtacat ggtaatggag tacatgcctg
     1021 gtggagacct tgtaaacctt atgagtaatt atgatgtgcc tgaaaaatgg gccaaatttt
     1081 acactgctga agttgttctt gctctggatg caatacactc catgggttta atacacagag
     1141 atgtgaagcc tgacaacatg ctcttggata aacatggaca tctaaaatta gcagattttg
     1201 gcacgtgtat gaagatggat gaaacaggca tggtacattg tgatacagca gttggaacac
     1261 cggattatat atcacctgag gttctgaaat cacaaggggg tgatggtttc tatgggcgag
     1321 aatgtgattg gtggtctgta ggtgttttcc tttatgagat gctagtgggg gatactccat
     1381 tttatgcgga ttcacttgta ggaacatata gcaaaattat ggatcataag aattcactgt
     1441 gtttccctga agatgcagaa atttccaaac atgcaaagaa tctcatctgt gctttcttaa
     1501 cagataggga ggtacgactt gggagaaatg gggtggaaga aatcagacag catcctttct
     1561 ttaagaatga tcagtggcat tgggataaca taagagaaac ggcagctcct gtagtacctg
     1621 aactcagcag tgacatagac agcagcaatt tcgatgacat tgaagatgac aaaggagatg
     1681 tagaaacctt cccaattcct aaagcttttg ttggaaatca gctgcctttc atcggattta
     1741 cctactatag agaaaattta ttattaagtg actctccatc ttgtagagaa actgattcca
     1801 tacaatcaag gaaaaatgaa gaaagtcaag agattcagaa aaaactgtat acattagaag
     1861 aacatcttag caatgagatg caagccaaag aggaactgga acagaagtgc aaatctgtta
     1921 atactcgcct agaaaaaaca gcaaaggagc tagaagagga gattacctta cggaaaagtg
     1981 tggaatcagc attaagacag ttagaaagag aaaaggcgct tcttcagcac aaaaatgcag
     2041 aatatcagag gaaagctgat catgaagcag acaaaaaacg aaatttggaa aatgatgtta
     2101 acagcttaaa agatcaactt gaagatttga aaaaaagaaa tcaaaactct caaatatcca
     2161 ctgagaaagt gaatcaactc cagagacaac tggatgaaac caatgcttta ctgcgaacag
     2221 agtctgatac tgcagcccgg ttaaggaaaa cccaggcaga aagttcaaaa cagattcagc
     2281 agctggaatc taacaataga gatctacaag ataaaaactg cctgctggag actgccaagt
     2341 taaaacttga aaaggaattt atcaatcttc agtcagctct agaatctgaa aggagggatc
     2401 gaacccatgg atcagagata attaatgatt tacaaggtag aatatgtggc ctagaagaag
     2461 atttaaagaa cggcaaaatc ttactagcga aagtagaact ggagaagaga caacttcagg
     2521 agagatttac tgatttggaa aaggaaaaaa gcaacatgga aatagatatg acataccaac
     2581 taaaagttat acagcagagc ctagaacaag aagaagctga acataaggcc acaaaggcac
     2641 gactagcaga taaaaataag atctatgagt ccatcgaaga agccaaatca gaagccatga
     2701 aagaaatgga gaagaagctc ttggaggaaa gaactttaaa acagaaagtg gagaacctat
     2761 tgctagaagc tgagaaaaga tgttctctat tagactgtga cctcaaacag tcacagcaga
     2821 aaataaatga gctccttaaa cagaaagatg tgctaaatga ggatgttaga aacctgacat
     2881 taaaaataga gcaagaaact cagaagcgct gccttacaca aaatgacctg aagatgcaaa
     2941 cacaacaggt taacacacta aaaatgtcag aaaagcagtt aaagcaagaa aataaccatc
     3001 tcatggaaat gaaaatgaac ttggaaaaac aaaatgctga acttcgaaaa gaacgtcagg
     3061 atgcagatgg gcaaatgaaa gagctccagg atcagctcga agcagaacag tatttctcaa
     3121 ccctttataa aacacaagtt agggagctta aagaagaatg tgaagaaaag accaaacttg
     3181 gtaaagaatt gcagcagaag aaacaggaat tacaggatga acgggactct ttggctgccc
     3241 aactggagat caccttgacc aaagcagatt ctgagcaact ggctcgttca attgctgaag
     3301 aacaatattc tgatttggaa aaagagaaga tcatgaaaga gctggagatc aaagagatga
     3361 tggctagaca caaacaggaa cttacggaaa aagatgctac aattgcttct cttgaggaaa
     3421 ctaataggac actaactagt gatgttgcca atcttgcaaa tgagaaagaa gaattaaata
     3481 acaaattgaa agatgttcaa gagcaactgt caagattgaa agatgaagaa ataagcgcag
     3541 cagctattaa agcacagttt gagaagcagc tattaacaga aagaacactc aaaactcaag
     3601 ctgtgaataa gttggctgag atcatgaatc gaaaagaacc tgtcaagcgt ggtaatgaca
     3661 cagatgtgcg gagaaaagag aaggagaata gaaagctaca tatggagctt aaatctgaac
     3721 gtgagaaatt gacccagcag atgatcaagt atcagaaaga actgaatgaa atgcaggcac
     3781 aaatagctga agagagccag attcgaattg aactgcagat gacattggac agtaaagaca
     3841 gtgacattga gcagctgcgg tcacaactcc aagccttgca tattggtctg gatagttcca
     3901 gtataggcag tggaccaggg gatgctgagg cagatgatgg gtttccagaa tcaagattag
     3961 aaggatggct ttcattgcct gtacgaaaca acactaagaa atttggatgg gttaaaaagt
     4021 atgtgattgt aagcagtaag aagattcttt tctatgacag tgaacaagat aaagaacaat
     4081 ccaatcctta catggtttta gatatagaca agttatttca tgtccgacca gttacacaga
     4141 cagatgtgta tagagcagat gctaaagaaa ttccaaggat attccagatt ctgtatgcca
     4201 atgaaggaga aagtaagaag gaacaagaat ttccagtgga gccagttgga gaaaaatcta
     4261 attatatttg ccacaaggga catgagttta ttcctactct ttatcatttc ccaaccaact
     4321 gtgaggcttg tatgaagccc ctgtggcaca tgtttaagcc tcctcctgct ttggagtgcc
     4381 gccgttgcca tattaagtgt cataaagatc atatggacaa aaaggaggag attatagcac
     4441 cttgcaaagt atattatgat atttcaacgg caaagaatct gttattacta gcaaattcta
     4501 cagaagagca gcagaagtgg gttagtcggt tggtgaaaaa gatacctaaa aagcccccag
     4561 ctccagaccc ttttgcccga tcatctccta gaacttcaat gaagatacag caaaaccagt
     4621 ctattagacg gccaagtcga cagcttgccc caaacaaacc tagctaactg ccttctatga
     4681 aagcagtcat tattcaaggt gatcgtattc ttccagtgaa aacaagactg aaatatgatg
     4741 gcccaaaatt tattaaaaag ctatattttc ctgagagact gatacataca ctcatacata
     4801 tatgtgttcc ccttttccct gtaatataaa ttacaaatct gggctccttt gaagcaacag
     4861 gttgaaccaa caatgattgg ttgatagact aaggatatat gcaactcttc cagacttttc
     4921 cataaagctc tctcggcagt cgctcacact acaatgcaca caaggattga gaagagttaa
     4981 aggctaaaga aaacatcttt tctagcttca acagagaggt ttcaccagca catttaccag
     5041 aagaatctgg gaatggattc cactacagtg atattgactg catctttaag aagtgaccat
     5101 tatactgtgt atatatatat aaacacacac acatatatat atatatatat agtactctaa
     5161 tactgcaaga aggtttttta aacttcccac tttatttttt atacacatta atcagatatc
     5221 attacttgct gcagttgcaa ctatgcactt gtataaagcc ataatgttgg agtttatatc
     5281 actcattcct gtgtacctga tggaagttgc atgttcatgt ttaagcagtt actgtaacaa
     5341 gaagtttaaa gttaattata tcagtttcct aatgcttcat gataggcaac tttacccatt
     5401 ttgaatgcct taatttaatt tttttcaaag tctcagccct gtctgtatta aaaaacaaaa
     5461 aaagcgttta ccagctctta ggatgtaaac tagctttgtg gaagataaat cgtgcactat
     5521 ttttacacat aaatagttat atcaatgtca gcctattttg attaacaaat gtttttaaag
     5581 tattattggt tatagaaaca ataatggatg gtgttggaac taatatatcc ttgatgtctg
     5641 tctattattc attcaactct ttttacagac ctcagtatta gtctgtgact acaaaatatt
     5701 ttatttgctt taaatttgct ggctacccta gatgtgtttt tattcctggt aaagacattt
     5761 gtgattacat tttcacactt aagattcaaa atttttccca aatataaaga aaactaagac
     5821 agactgtaga tgcattttaa atatttaaat atgatcctca gacatgcagc tgtgtgtggc
     5881 agtattttag taccgggtta agaaaactgg caactgggaa gaagtggcct caaaggcact
     5941 taatttgatt tttatttttt aaatgctgtc aaagttacag tttacgcagg acattcttgc
     6001 cgtattctca tgatcccaga taagtgtgtg ttttatactg caacaatatg cagcaatggt
     6061 aagcgtaaag tttttttttt gtttttgttt ttttttatat tatgaagtct tttaacagtc
     6121 tctctttata taaatacaca gagtttggta tgatatttaa atacatcatc tggccaggca
     6181 tggtggctta cgcctgtaat cctagcactt tgggaggcca agacgggcgg atcacctgag
     6241 gtgaggagtt caagaccagc ctgcccaaca tagtgaaact ccgtctctac caatatacaa
     6301 aaattagccg ggcatgatgg tggtggcctg taatcccagc tacttgggag gctgagacag
     6361 gagaatcgct tgaacccagg agacggtggt tgcagtgagc gaagatcgag ccactgcact
     6421 ccagcctggg cagctgaaca agactccgtc tcaaaaaaaa aaaaaaaaaa aagtcatcta
     6481 atatttcgat gaaggaaata gacatttttc attgattccc tctgatgtca gttagagata
     6541 tttaaaaagt tagatcccca taatagatac tattgtggaa actgaagagt agctactggc
     6601 ctactgatag actgaaactg gttttgttat tcttaacgtt ctccagtctg cactctgctg
     6661 tgctgtctgt gctcagacta gacgtgcatg ccgtttcctt tgcaggtgtg ttctgcagct
     6721 ctggttttgg gggatagctc ttttttaagt aagattaaaa aatgaaaaat atataaattt
     6781 atatagaata aatatttcca ttcattagag ttgttaaaag gagactgaat taatatttta
     6841 tataaagatt ttgttacatt atgggaggtt ctatcctatt ctgaattgga gagtatatat
     6901 atatatatat atatatatat atttttaata aactttatta aggtgaaata atttcaagtt
     6961 tacatatgag taactgaaat atttgagtaa aaaaggcaaa agtttaaatt tggaatgctg
     7021 tatatattag agaatatggg gaatcatggt gtgaccaact gtacaaggaa gattcatatg
     7081 aaggtcttag gggaaaaaga taattctgaa gggtgaaaaa aaaatctctc ccatggagat
     7141 tcttcacatt atatggtttg acataagggt ttataaggtt gtttgtccaa ctaatgaaaa
     7201 tgcgcttaat aaaaaattct gaccttgggt aagaattcgc tttttaccgc attgccgctt
     7261 tcatgtacat acatgagtgc atgcacctat caatgtgttt tcttcccaac acttattaaa
     7321 tataaaacaa cctgattttg tgctcgtttc tcgtttttct tgaggccctg aagcttccaa
     7381 gataaatata agactaaaat ttgcttgttc cttcacacct gatttctcct agaaccataa
     7441 atacctccta tggggaacta agaaatcatg aatactggga ttttcttatt ggatttttta
     7501 cttttgtttt gtggggataa cgagacagaa gtgaaaggaa aactggtgcc ggtattactg
     7561 ttgaaattaa tgtacagtga atgtttttaa agtattaatt ttaaaaaacc atgttctcta
     7621 gaaacaagtt ttaaaaatct atatgaaagt tttaaaatgt tccagctaac taatggataa
     7681 gcataccagt aaagccaaag taaaggttag tgcctttaaa actaacctgt gttagagtta
     7741 catgaatctg gctctaaagt atctattttg catccattta tatatagatc ttaaacagaa
     7801 atactctagg ttgccacacc acagttttaa gaagttatgc tgctgctgtt actctcttct
     7861 aggattctgg tggtaaagta gagaaactcg gacactatat taaccttact gaaacatgct
     7921 ttaaatcgtg tctaaaatct gatttgagca agtcactgcc gagcttctga gtctgcattg
     7981 acactgctac tgactcacct ctccttccct ggaataaggc ttcagcttcc ctatgctgtg
     8041 aagacaccat ttgtaagcac tttgaacata agcactgtgt gacagcgctg gatatcatgc
     8101 ttgggggggg gcctctccct gactcccatc cccataataa tgagttttgt ttggaattgt
     8161 aataagtaat gaactgcatg gtttagaaaa ttataaatat ttgatcagct gtcctctctg
     8221 aaaaaaattg cacccctttt tcttttttaa aattttgttg catcttcgta gtaatgtaat
     8281 tgtattttat gcctgctttt ttataaaaaa ataaaataaa ataaatggag acataagcat
     8341 tttca
//
