LOCUS       NM_019055               4294 bp    mRNA    linear   PRI 13-MAY-2024
DEFINITION  Homo sapiens roundabout guidance receptor 4 (ROBO4), transcript
            variant 1, mRNA.
ACCESSION   NM_019055
VERSION     NM_019055.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Chen,R., Wang,Y., Zhang,Z., Wang,X., Li,Y., Wang,M., Wang,H.,
            Dong,M., Zhou,Q. and Yang,L.
  TITLE     The Role of SLIT3-ROBO4 Signaling in Endoplasmic Reticulum
            Stress-Induced Delayed Corneal Epithelial and Nerve Regeneration
  JOURNAL   Invest Ophthalmol Vis Sci 65 (5), 8 (2024)
   PUBMED   38700874
  REMARK    GeneRIF: The Role of SLIT3-ROBO4 Signaling in Endoplasmic Reticulum
            Stress-Induced Delayed Corneal Epithelial and Nerve Regeneration.
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Zhao,L., Xu,H., Liu,X., Cheng,Y. and Xie,J.
  TITLE     The role of TET2-mediated ROBO4 hypomethylation in the development
            of diabetic retinopathy
  JOURNAL   J Transl Med 21 (1), 455 (2023)
   PUBMED   37430272
  REMARK    GeneRIF: The role of TET2-mediated ROBO4 hypomethylation in the
            development of diabetic retinopathy.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4294)
  AUTHORS   Yamanaka,M., Hayashi,M., Sonohara,F., Yamada,S., Tanaka,H.,
            Sakai,A., Mii,S., Kobayashi,D., Kurimoto,K., Tanaka,N., Inokawa,Y.,
            Takami,H., Hattori,N., Kanda,M., Tanaka,C., Nakayama,G., Koike,M.
            and Kodera,Y.
  TITLE     Downregulation of ROBO4 in Pancreatic Cancer Serves as a Biomarker
            of Poor Prognosis and Indicates Increased Cell Motility and
            Proliferation Through Activation of MMP-9
  JOURNAL   Ann Surg Oncol 29 (11), 7180-7189 (2022)
   PUBMED   35726111
  REMARK    GeneRIF: Downregulation of ROBO4 in Pancreatic Cancer Serves as a
            Biomarker of Poor Prognosis and Indicates Increased Cell Motility
            and Proliferation Through Activation of MMP-9.
REFERENCE   4  (bases 1 to 4294)
  AUTHORS   Luo,Y., Li,Y., Peng,H. and Zhao,Y.
  TITLE     miR-140-5p regulates vascular smooth muscle cell viability,
            migration and apoptosis by targeting ROBO4 gene expression in
            atherosclerosis
  JOURNAL   Mol Med Rep 23 (3) (2021)
   PUBMED   33495827
  REMARK    GeneRIF: miR-140-5p regulates vascular smooth muscle cell
            viability, migration and apoptosis by targeting ROBO4 gene
            expression in atherosclerosis.
REFERENCE   5  (bases 1 to 4294)
  AUTHORS   Sengupta,D., Bhattacharya,G., Ganguli,S. and Sengupta,M.
  TITLE     Structural insights and evaluation of the potential impact of
            missense variants on the interactions of SLIT2 with ROBO1/4 in
            cancer progression
  JOURNAL   Sci Rep 10 (1), 21909 (2020)
   PUBMED   33318575
  REMARK    GeneRIF: Structural insights and evaluation of the potential impact
            of missense variants on the interactions of SLIT2 with ROBO1/4 in
            cancer progression.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4294)
  AUTHORS   Seth,P., Lin,Y., Hanai,J., Shivalingappa,V., Duyao,M.P. and
            Sukhatme,V.P.
  TITLE     Magic roundabout, a tumor endothelial marker: expression and
            signaling
  JOURNAL   Biochem Biophys Res Commun 332 (2), 533-541 (2005)
   PUBMED   15894287
  REMARK    GeneRIF: We therefore hypothesize that expression of MRB is
            involved in regulating the migration of endothelial cells during
            tumor angiogenesis.
REFERENCE   7  (bases 1 to 4294)
  AUTHORS   Bedell,V.M., Yeo,S.Y., Park,K.W., Chung,J., Seth,P.,
            Shivalingappa,V., Zhao,J., Obara,T., Sukhatme,V.P., Drummond,I.A.,
            Li,D.Y. and Ramchandran,R.
  TITLE     roundabout4 is essential for angiogenesis in vivo
  JOURNAL   Proc Natl Acad Sci U S A 102 (18), 6373-6378 (2005)
   PUBMED   15849270
REFERENCE   8  (bases 1 to 4294)
  AUTHORS   Schmucker,D.
  TITLE     Downstream of guidance receptors: entering the baroque period of
            axon guidance signaling
  JOURNAL   Neuron 40 (1), 4-6 (2003)
   PUBMED   14527427
  REMARK    Review article
REFERENCE   9  (bases 1 to 4294)
  AUTHORS   Park,K.W., Morrison,C.M., Sorensen,L.K., Jones,C.A., Rao,Y.,
            Chien,C.B., Wu,J.Y., Urness,L.D. and Li,D.Y.
  TITLE     Robo4 is a vascular-specific receptor that inhibits endothelial
            migration
  JOURNAL   Dev Biol 261 (1), 251-267 (2003)
   PUBMED   12941633
REFERENCE   10 (bases 1 to 4294)
  AUTHORS   Huminiecki,L., Gorn,M., Suchting,S., Poulsom,R. and Bicknell,R.
  TITLE     Magic roundabout is a new member of the roundabout receptor family
            that is endothelial specific and expressed at sites of active
            angiogenesis
  JOURNAL   Genomics 79 (4), 547-552 (2002)
   PUBMED   11944987
  REMARK    GeneRIF: roundabout gene family extends beyond neuronal tissue and
            that roundabout/slit interactions are likely to have a role in
            angiogenesis
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DC424813.1, AF361473.2 and AP003501.2.
            
            On May 17, 2019 this sequence version replaced NM_019055.5.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK027852.1, AF361473.2 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000306534.8/ ENSP00000304945.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-24                DC424813.1         72-95
            25-3767             AF361473.2         1-3743
            3768-4294           AP003501.2         18771-19297         c
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q24.2"
     gene            1..4294
                     /gene="ROBO4"
                     /gene_synonym="AOVD3; ECSM4; MRB"
                     /note="roundabout guidance receptor 4"
                     /db_xref="GeneID:54538"
                     /db_xref="HGNC:HGNC:17985"
                     /db_xref="MIM:607528"
     exon            1..140
                     /gene="ROBO4"
                     /gene_synonym="AOVD3; ECSM4; MRB"
                     /inference="alignment:Splign:2.1.0"
     CDS             71..3094
                     /gene="ROBO4"
                     /gene_synonym="AOVD3; ECSM4; MRB"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; roundabout homolog 4, magic roundabout;
                     roundabout, axon guidance receptor, homolog 4"
                     /codon_start=1
                     /product="roundabout homolog 4 isoform 1 precursor"
                     /protein_id="NP_061928.4"
                     /db_xref="CCDS:CCDS8455.1"
                     /db_xref="GeneID:54538"
                     /db_xref="HGNC:HGNC:17985"
                     /db_xref="MIM:607528"
                     /translation="MGSGGDSLLGGRGSLPLLLLLIMGGMAQDSPPQILVHPQDQLFQ
                     GPGPARMSCQASGQPPPTIRWLLNGQPLSMVPPDPHHLLPDGTLLLLQPPARGHAHDG
                     QALSTDLGVYTCEASNRLGTAVSRGARLSVAVLREDFQIQPRDMVAVVGEQFTLECGP
                     PWGHPEPTVSWWKDGKPLALQPGRHTVSGGSLLMARAEKSDEGTYMCVATNSAGHRES
                     RAARVSIQEPQDYTEPVELLAVRIQLENVTLLNPDPAEGPKPRPAVWLSWKVSGPAAP
                     AQSYTALFRTQTAPGGQGAPWAEELLAGWQSAELGGLHWGQDYEFKVRPSSGRARGPD
                     SNVLLLRLPEKVPSAPPQEVTLKPGNGTVFVSWVPPPAENHNGIIRGYQVWSLGNTSL
                     PPANWTVVGEQTQLEIATHMPGSYCVQVAAVTGAGAGEPSRPVCLLLEQAMERATQEP
                     SEHGPWTLEQLRATLKRPEVIATCGVALWLLLLGTAVCIHRRRRARVHLGPGLYRYTS
                     EDAILKHRMDHSDSQWLADTWRSTSGSRDLSSSSSLSSRLGADARDPLDCRRSLLSWD
                     SRSPGVPLLPDTSTFYGSLIAELPSSTPARPSPQVPAVRRLPPQLAQLSSPCSSSDSL
                     CSRRGLSSPRLSLAPAEAWKAKKKQELQHANSSPLLRGSHSLELRACELGNRGSKNLS
                     QSPGAVPQALVAWRALGPKLLSSSNELVTRHLPPAPLFPHETPPTQSQQTQPPVAPQA
                     PSSILLPAAPIPILSPCSPPSPQASSLSGPSPASSRLSSSSLSSLGEDQDSVLTPEEV
                     ALCLELSEGEETPRNSVSPMPRAPSPPTTYGYISVPTASEFTDMGRTGGGVGPKGGVL
                     LCPPRPCLTPTPSEGSLANGWGSASEDNAASARASLVSSSDGSFLADAHFARALAVAV
                     DSFGFGLEPREADCVFIDASSPPSPRDEIFLTPNLSLPLWEWRPDWLEDMEVSHTQRL
                     GRGMPPWPPDSQISSQRSQLHCRMPKAGASPVDYS"
     exon            141..470
                     /gene="ROBO4"
                     /gene_synonym="AOVD3; ECSM4; MRB"
                     /inference="alignment:Splign:2.1.0"
     exon            471..628
                     /gene="ROBO4"
                     /gene_synonym="AOVD3; ECSM4; MRB"
                     /inference="alignment:Splign:2.1.0"
     exon            629..749
                     /gene="ROBO4"
                     /gene_synonym="AOVD3; ECSM4; MRB"
                     /inference="alignment:Splign:2.1.0"
     exon            750..877
                     /gene="ROBO4"
                     /gene_synonym="AOVD3; ECSM4; MRB"
                     /inference="alignment:Splign:2.1.0"
     exon            878..1106
                     /gene="ROBO4"
                     /gene_synonym="AOVD3; ECSM4; MRB"
                     /inference="alignment:Splign:2.1.0"
     exon            1107..1219
                     /gene="ROBO4"
                     /gene_synonym="AOVD3; ECSM4; MRB"
                     /inference="alignment:Splign:2.1.0"
     exon            1220..1388
                     /gene="ROBO4"
                     /gene_synonym="AOVD3; ECSM4; MRB"
                     /inference="alignment:Splign:2.1.0"
     exon            1389..1574
                     /gene="ROBO4"
                     /gene_synonym="AOVD3; ECSM4; MRB"
                     /inference="alignment:Splign:2.1.0"
     exon            1575..1617
                     /gene="ROBO4"
                     /gene_synonym="AOVD3; ECSM4; MRB"
                     /inference="alignment:Splign:2.1.0"
     exon            1618..1754
                     /gene="ROBO4"
                     /gene_synonym="AOVD3; ECSM4; MRB"
                     /inference="alignment:Splign:2.1.0"
     exon            1755..2018
                     /gene="ROBO4"
                     /gene_synonym="AOVD3; ECSM4; MRB"
                     /inference="alignment:Splign:2.1.0"
     exon            2019..2126
                     /gene="ROBO4"
                     /gene_synonym="AOVD3; ECSM4; MRB"
                     /inference="alignment:Splign:2.1.0"
     exon            2127..2268
                     /gene="ROBO4"
                     /gene_synonym="AOVD3; ECSM4; MRB"
                     /inference="alignment:Splign:2.1.0"
     exon            2269..2505
                     /gene="ROBO4"
                     /gene_synonym="AOVD3; ECSM4; MRB"
                     /inference="alignment:Splign:2.1.0"
     exon            2506..2864
                     /gene="ROBO4"
                     /gene_synonym="AOVD3; ECSM4; MRB"
                     /inference="alignment:Splign:2.1.0"
     exon            2865..3071
                     /gene="ROBO4"
                     /gene_synonym="AOVD3; ECSM4; MRB"
                     /inference="alignment:Splign:2.1.0"
     exon            3072..4294
                     /gene="ROBO4"
                     /gene_synonym="AOVD3; ECSM4; MRB"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3733..3738
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ROBO4"
                     /gene_synonym="AOVD3; ECSM4; MRB"
                     /note="hexamer: AAGAAA"
     polyA_site      3767
                     /gene="ROBO4"
                     /gene_synonym="AOVD3; ECSM4; MRB"
                     /note="major polyA site"
     regulatory      3898..3903
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ROBO4"
                     /gene_synonym="AOVD3; ECSM4; MRB"
                     /note="hexamer: ATTAAA"
     polyA_site      3928
                     /gene="ROBO4"
                     /gene_synonym="AOVD3; ECSM4; MRB"
     regulatory      3995..4000
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ROBO4"
                     /gene_synonym="AOVD3; ECSM4; MRB"
                     /note="hexamer: ATTAAA"
     polyA_site      4018
                     /gene="ROBO4"
                     /gene_synonym="AOVD3; ECSM4; MRB"
ORIGIN      
        1 ccctcttcac tgtgagctca gagcagcagg acaaagtgct cgggacaagg acatagggct
       61 gagagtagcc atgggctctg gaggagacag cctcctgggg ggcaggggtt ccctgcctct
      121 gctgctcctg ctcatcatgg gaggcatggc tcaggactcc ccgccccaga tcctagtcca
      181 cccccaggac cagctgttcc agggccctgg ccctgccagg atgagctgcc aagcctcagg
      241 ccagccacct cccaccatcc gctggttgct gaatgggcag cccctgagca tggtgccccc
      301 agacccacac cacctcctgc ctgatgggac ccttctgctg ctacagcccc ctgcccgggg
      361 acatgcccac gatggccagg ccctgtccac agacctgggt gtctacacat gtgaggccag
      421 caaccggctt ggcacggcag tcagcagagg cgctcggctg tctgtggctg tcctccggga
      481 ggatttccag atccagcctc gggacatggt ggctgtggtg ggtgagcagt ttactctgga
      541 atgtgggccg ccctggggcc acccagagcc cacagtctca tggtggaaag atgggaaacc
      601 cctggccctc cagcccggaa ggcacacagt gtccgggggg tccctgctga tggcaagagc
      661 agagaagagt gacgaaggga cctacatgtg tgtggccacc aacagcgcag gacataggga
      721 gagccgcgca gcccgggttt ccatccagga gccccaggac tacacggagc ctgtggagct
      781 tctggctgtg cgaattcagc tggaaaatgt gacactgctg aacccggatc ctgcagaggg
      841 ccccaagcct agaccggcgg tgtggctcag ctggaaggtc agtggccctg ctgcgcctgc
      901 ccaatcttac acggccttgt tcaggaccca gactgccccg ggaggccagg gagctccgtg
      961 ggcagaggag ctgctggccg gctggcagag cgcagagctt ggaggcctcc actggggcca
     1021 agactacgag ttcaaagtga gaccatcctc tggccgggct cgaggccctg acagcaacgt
     1081 gctgctcctg aggctgccgg aaaaagtgcc cagtgcccca cctcaggaag tgactctaaa
     1141 gcctggcaat ggcactgtct ttgtgagctg ggtcccacca cctgctgaaa accacaatgg
     1201 catcatccgt ggctaccagg tctggagcct gggcaacaca tcactgccac cagccaactg
     1261 gactgtagtt ggtgagcaga cccagctgga aatcgccacc catatgccag gctcctactg
     1321 cgtgcaagtg gctgcagtca ctggtgctgg agctggggag cccagtagac ctgtctgcct
     1381 ccttttagag caggccatgg agcgagccac ccaagaaccc agtgagcatg gtccctggac
     1441 cctggagcag ctgagggcta ccttgaagcg gcctgaggtc attgccacct gcggtgttgc
     1501 actctggctg ctgcttctgg gcaccgccgt gtgtatccac cgccggcgcc gagctagggt
     1561 gcacctgggc ccaggtctgt acagatatac cagtgaggat gccatcctaa aacacaggat
     1621 ggatcacagt gactcccagt ggttggcaga cacttggcgt tccacctctg gctctcggga
     1681 cctgagcagc agcagcagcc tcagcagtcg gctgggggcg gatgcccggg acccactaga
     1741 ctgtcgtcgc tccttgctct cctgggactc ccgaagcccc ggcgtgcccc tgcttccaga
     1801 caccagcact ttttatggct ccctcatcgc tgagctgccc tccagtaccc cagccaggcc
     1861 aagtccccag gtcccagctg tcaggcgcct cccaccccag ctggcccagc tctccagccc
     1921 ctgttccagc tcagacagcc tctgcagccg caggggactc tcttctcccc gcttgtctct
     1981 ggcccctgca gaggcttgga aggccaaaaa gaagcaggag ctgcagcatg ccaacagttc
     2041 cccactgctc cggggcagcc actccttgga gctccgggcc tgtgagttag gaaatagagg
     2101 ttccaagaac ctttcccaaa gcccaggagc tgtgccccaa gctctggttg cctggcgggc
     2161 cctgggaccg aaactcctca gctcctcaaa tgagctggtt actcgtcatc tccctccagc
     2221 acccctcttt cctcatgaaa ctcccccaac tcagagtcaa cagacccagc ctccggtggc
     2281 accacaggct ccctcctcca tcctgctgcc agcagccccc atccccatcc ttagcccctg
     2341 cagtccccct agcccccagg cctcttccct ctctggcccc agcccagctt ccagtcgcct
     2401 gtccagctcc tcactgtcat ccctggggga ggatcaagac agcgtgctga cccctgagga
     2461 ggtagccctg tgcttggaac tcagtgaggg tgaggagact cccaggaaca gcgtctctcc
     2521 catgccaagg gctccttcac cccccaccac ctatgggtac atcagcgtcc caacagcctc
     2581 agagttcacg gacatgggca ggactggagg aggggtgggg cccaaggggg gagtcttgct
     2641 gtgcccacct cggccctgcc tcacccccac ccccagcgag ggctccttag ccaatggttg
     2701 gggctcagcc tctgaggaca atgccgccag cgccagagcc agccttgtca gctcctccga
     2761 tggctccttc ctcgctgatg ctcactttgc ccgggccctg gcagtggctg tggatagctt
     2821 tggtttcggt ctagagccca gggaggcaga ctgcgtcttc atagatgcct catcacctcc
     2881 ctccccacgg gatgagatct tcctgacccc caacctctcc ctgcccctgt gggagtggag
     2941 gccagactgg ttggaagaca tggaggtcag ccacacccag cggctgggaa gggggatgcc
     3001 tccctggccc cctgactctc agatctcttc ccagagaagt cagctccact gtcgtatgcc
     3061 caaggctggt gcttctcctg tagattactc ctgaaccgtg tccctgagac ttcccagacg
     3121 ggaatcagaa ccacttctcc tgtccaccca caagacctgg gctgtggtgt gtgggtcttg
     3181 gcctgtgttt ctctgcagct ggggtccacc ttcccaagcc tccagagagt tctccctcca
     3241 cgattgtgaa aacaaatgaa aacaaaatta gagcaaagct gacctggagc cctcagggag
     3301 caaaacatca tctccacctg actcctagcc actgctttct cctctgtgcc atccactccc
     3361 accaccaggt tgttttggcc tgaggagcag ccctgcctgc tgctcttccc ccaccatttg
     3421 gatcacagga agtggaggag ccagaggtgc ctttgtggag gacagcagtg gctgctggga
     3481 gagggctgtg gaggaaggag cttctcggag ccccctctca gccttacctg ggcccctcct
     3541 ctagagaaga gctcaactct ctcccaacct caccatggaa agaaaataat tatgaatgcc
     3601 actgaggcac tgaggcccta cctcatgcca aacaaagggt tcaaggctgg gtctagcgag
     3661 gatgctgaag gaagggaggt atgagaccgt aggtcaaaag caccatcctc gtactgttgt
     3721 cactatgagc ttaagaaatt tgataccata aaatggtaaa gacttgagtt ctgtgagatc
     3781 attccccgga gcaccatttt taggggagca cctggagaga tggcaagaat ttcctgagtt
     3841 aggcagggat caggcattca ttgacactca gggagtgtca cacatttctg ttctgcaatt
     3901 aaagggagaa tgaggttcat ccaccaaatt ttaagcagaa tataggaagg gcaggggtgg
     3961 ggagtttcag ggtctgctgg tcctggcaac ttatattaaa tccacctcct caagataagc
     4021 cttagggata tttatggctg agggaagtgg gatggtctaa ggcatgggga agggtgattg
     4081 gctgtgggga aaatgaagta ataggttcat tctgcccaag tgtacttggg gttcatggca
     4141 tttcataagg acatttgtgt agaaaattgt tttgctggct gggcacggtg gctcacgcct
     4201 gtaatcccag cactttggga ggcggaggta ggcagatcat gaggtcagga gatcgagacc
     4261 atcctggcta acatggtgaa accccgtctc tact
//
