LOCUS       NM_003800               4825 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens RNA guanylyltransferase and 5'-phosphatase (RNGTT),
            transcript variant 1, mRNA.
ACCESSION   NM_003800
VERSION     NM_003800.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Lombardi,O., Varshney,D., Phillips,N.M. and Cowling,V.H.
  TITLE     c-Myc deregulation induces mRNA capping enzyme dependency
  JOURNAL   Oncotarget 7 (50), 82273-82288 (2016)
   PUBMED   27756891
  REMARK    GeneRIF: c-Myc deregulation induces mRNA capping enzyme dependency
            in tumor cells.
REFERENCE   3  (bases 1 to 4825)
  AUTHORS   Mukherjee,C., Bakthavachalu,B. and Schoenberg,D.R.
  TITLE     The cytoplasmic capping complex assembles on adapter protein nck1
            bound to the proline-rich C-terminus of Mammalian capping enzyme
  JOURNAL   PLoS Biol 12 (8), e1001933 (2014)
   PUBMED   25137142
  REMARK    GeneRIF: These results identify a new domain of CE that is specific
            to its function in cytoplasmic capping, and a new role for Nck1 in
            regulating gene expression through its role as the scaffold for
            assembly of the cytoplasmic capping complex.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4825)
  AUTHORS   Picard-Jean,F., Bougie,I., Shuto,S. and Bisaillon,M.
  TITLE     The immunosuppressive agent mizoribine monophosphate is an
            inhibitor of the human RNA capping enzyme
  JOURNAL   PLoS One 8 (1), e54621 (2013)
   PUBMED   23349942
  REMARK    GeneRIF: Our biochemical studies provide the first insight that MZP
            can inhibit the formation of the RNA cap structure catalyzed by
            HCE.
REFERENCE   5  (bases 1 to 4825)
  AUTHORS   Chu,C., Das,K., Tyminski,J.R., Bauman,J.D., Guan,R., Qiu,W.,
            Montelione,G.T., Arnold,E. and Shatkin,A.J.
  TITLE     Structure of the guanylyltransferase domain of human mRNA capping
            enzyme
  JOURNAL   Proc Natl Acad Sci U S A 108 (25), 10104-10108 (2011)
   PUBMED   21636784
  REMARK    GeneRIF: Data show that human capping enzyme residues 229-567
            comprise the minimum enzymatically active human GTase (hGTase)
            domain and determine the structure by X-ray crystallography.
REFERENCE   6  (bases 1 to 4825)
  AUTHORS   Tsukamoto,T., Shibagaki,Y., Niikura,Y. and Mizumoto,K.
  TITLE     Cloning and characterization of three human cDNAs encoding mRNA
            (guanine-7-)-methyltransferase, an mRNA cap methylase
  JOURNAL   Biochem Biophys Res Commun 251 (1), 27-34 (1998)
   PUBMED   9790902
REFERENCE   7  (bases 1 to 4825)
  AUTHORS   Pillutla,R.C., Yue,Z., Maldonado,E. and Shatkin,A.J.
  TITLE     Recombinant human mRNA cap methyltransferase binds capping
            enzyme/RNA polymerase IIo complexes
  JOURNAL   J Biol Chem 273 (34), 21443-21446 (1998)
   PUBMED   9705270
REFERENCE   8  (bases 1 to 4825)
  AUTHORS   Yamada-Okabe,T., Doi,R., Shimmi,O., Arisawa,M. and Yamada-Okabe,H.
  TITLE     Isolation and characterization of a human cDNA for mRNA 5'-capping
            enzyme
  JOURNAL   Nucleic Acids Res 26 (7), 1700-1706 (1998)
   PUBMED   9512541
REFERENCE   9  (bases 1 to 4825)
  AUTHORS   Tsukamoto,T., Shibagaki,Y., Murakoshi,T., Suzuki,M., Nakamura,A.,
            Gotoh,H. and Mizumoto,K.
  TITLE     Cloning and characterization of two human cDNAs encoding the mRNA
            capping enzyme
  JOURNAL   Biochem Biophys Res Commun 243 (1), 101-108 (1998)
   PUBMED   9473487
REFERENCE   10 (bases 1 to 4825)
  AUTHORS   Yue,Z., Maldonado,E., Pillutla,R., Cho,H., Reinberg,D. and
            Shatkin,A.J.
  TITLE     Mammalian capping enzyme complements mutant Saccharomyces
            cerevisiae lacking mRNA guanylyltransferase and selectively binds
            the elongating form of RNA polymerase II
  JOURNAL   Proc Natl Acad Sci U S A 94 (24), 12898-12903 (1997)
   PUBMED   9371772
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BP256840.1, AB009022.1, BX537450.1, BC019954.1, AI193199.1 and
            BP396964.1.
            
            On Nov 22, 2018 this sequence version replaced NM_003800.4.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038197.1790820.1, BC019954.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000369485.9/ ENSP00000358497.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-209               BP256840.1         12-220
            210-2003            AB009022.1         1-1794
            2004-2934           BX537450.1         3203-4133
            2935-3141           BC019954.1         2913-3119
            3142-4450           BX537450.1         4341-5649
            4451-4643           AI193199.1         285-477             c
            4644-4825           BP396964.1         1-182
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q15"
     gene            1..4825
                     /gene="RNGTT"
                     /gene_synonym="CAP1A; hCAP; HCE; HCE1"
                     /note="RNA guanylyltransferase and 5'-phosphatase"
                     /db_xref="GeneID:8732"
                     /db_xref="HGNC:HGNC:10073"
                     /db_xref="MIM:603512"
     exon            1..273
                     /gene="RNGTT"
                     /gene_synonym="CAP1A; hCAP; HCE; HCE1"
                     /inference="alignment:Splign:2.1.0"
     CDS             210..2003
                     /gene="RNGTT"
                     /gene_synonym="CAP1A; hCAP; HCE; HCE1"
                     /EC_number="2.7.7.50"
                     /EC_number="3.6.1.74"
                     /note="isoform a is encoded by transcript variant 1;
                     mRNA-capping enzyme; HCAP1"
                     /codon_start=1
                     /product="mRNA-capping enzyme isoform a"
                     /protein_id="NP_003791.3"
                     /db_xref="CCDS:CCDS5017.1"
                     /db_xref="GeneID:8732"
                     /db_xref="HGNC:HGNC:10073"
                     /db_xref="MIM:603512"
                     /translation="MAHNKIPPRWLNCPRRGQPVAGRFLPLKTMLGPRYDSQVAEENR
                     FHPSMLSNYLKSLKVKMGLLVDLTNTSRFYDRNDIEKEGIKYIKLQCKGHGECPTTEN
                     TETFIRLCERFNERNPPELIGVHCTHGFNRTGFLICAFLVEKMDWSIEAAVATFAQAR
                     PPGIYKGDYLKELFRRYGDIEEAPPPPLLPDWCFEDDEDEDEDEDGKKESEPGSSASF
                     GKRRKERLKLGAIFLEGVTVKGVTQVTTQPKLGEVQQKCHQFCGWEGSGFPGAQPVSM
                     DKQNIKLLDLKPYKVSWKADGTRYMMLIDGTNEVFMIDRDNSVFHVSNLEFPFRKDLR
                     MHLSNTLLDGEMIIDRVNGQAVPRYLIYDIIKFNSQPVGDCDFNVRLQCIEREIISPR
                     HEKMKTGLIDKTQEPFSVRNKPFFDICTSRKLLEGNFAKEVSHEMDGLIFQPTGKYKP
                     GRCDDILKWKPPSLNSVDFRLKITRMGGEGLLPQNVGLLYVGGYERPFAQIKVTKELK
                     QYDNKIIECKFENNSWVFMRQRTDKSFPNAYNTAMAVCNSISNPVTKEMLFEFIDRCT
                     AASQGQKRKHHLDPDTELMPPPPPKRPRPLT"
     exon            274..383
                     /gene="RNGTT"
                     /gene_synonym="CAP1A; hCAP; HCE; HCE1"
                     /inference="alignment:Splign:2.1.0"
     exon            384..487
                     /gene="RNGTT"
                     /gene_synonym="CAP1A; hCAP; HCE; HCE1"
                     /inference="alignment:Splign:2.1.0"
     exon            488..576
                     /gene="RNGTT"
                     /gene_synonym="CAP1A; hCAP; HCE; HCE1"
                     /inference="alignment:Splign:2.1.0"
     exon            577..652
                     /gene="RNGTT"
                     /gene_synonym="CAP1A; hCAP; HCE; HCE1"
                     /inference="alignment:Splign:2.1.0"
     exon            653..893
                     /gene="RNGTT"
                     /gene_synonym="CAP1A; hCAP; HCE; HCE1"
                     /inference="alignment:Splign:2.1.0"
     exon            894..1003
                     /gene="RNGTT"
                     /gene_synonym="CAP1A; hCAP; HCE; HCE1"
                     /inference="alignment:Splign:2.1.0"
     exon            1004..1105
                     /gene="RNGTT"
                     /gene_synonym="CAP1A; hCAP; HCE; HCE1"
                     /inference="alignment:Splign:2.1.0"
     exon            1106..1241
                     /gene="RNGTT"
                     /gene_synonym="CAP1A; hCAP; HCE; HCE1"
                     /inference="alignment:Splign:2.1.0"
     exon            1242..1313
                     /gene="RNGTT"
                     /gene_synonym="CAP1A; hCAP; HCE; HCE1"
                     /inference="alignment:Splign:2.1.0"
     exon            1314..1478
                     /gene="RNGTT"
                     /gene_synonym="CAP1A; hCAP; HCE; HCE1"
                     /inference="alignment:Splign:2.1.0"
     exon            1479..1547
                     /gene="RNGTT"
                     /gene_synonym="CAP1A; hCAP; HCE; HCE1"
                     /inference="alignment:Splign:2.1.0"
     exon            1548..1648
                     /gene="RNGTT"
                     /gene_synonym="CAP1A; hCAP; HCE; HCE1"
                     /inference="alignment:Splign:2.1.0"
     exon            1649..1715
                     /gene="RNGTT"
                     /gene_synonym="CAP1A; hCAP; HCE; HCE1"
                     /inference="alignment:Splign:2.1.0"
     exon            1716..1839
                     /gene="RNGTT"
                     /gene_synonym="CAP1A; hCAP; HCE; HCE1"
                     /inference="alignment:Splign:2.1.0"
     exon            1840..4825
                     /gene="RNGTT"
                     /gene_synonym="CAP1A; hCAP; HCE; HCE1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4428..4433
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RNGTT"
                     /gene_synonym="CAP1A; hCAP; HCE; HCE1"
                     /note="hexamer: AATAAA"
     polyA_site      4450
                     /gene="RNGTT"
                     /gene_synonym="CAP1A; hCAP; HCE; HCE1"
                     /note="major polyA site"
     regulatory      4807..4812
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RNGTT"
                     /gene_synonym="CAP1A; hCAP; HCE; HCE1"
                     /note="hexamer: AATAAA"
     polyA_site      4825
                     /gene="RNGTT"
                     /gene_synonym="CAP1A; hCAP; HCE; HCE1"
ORIGIN      
        1 gcattgtggg ttctcctgga gctgtggagt tgatcctgaa tgaaagtggc gcgccgcccc
       61 tgacgttacc cggatcggag aggttggaat tcagattacg gctgcgattc gggtgtctcg
      121 gaccccggtg tgcaccggac cacggggagg cggctccaaa ggcgcggtga acgttggtga
      181 gggagggcag ctctgcgcag ccccaagaca tggctcacaa caagatcccg ccgcggtggc
      241 tgaactgtcc ccggcgcggc cagccggtgg caggaagatt cttacctctg aagacaatgt
      301 taggaccaag atatgatagt caagttgctg aagaaaatcg gttccatccc agcatgctct
      361 caaattacct aaagagccta aaggttaaaa tgggcttgtt ggtggacctg acaaatactt
      421 caaggttcta tgaccgaaat gacatagaaa aagaaggaat caaatatata aaacttcagt
      481 gtaaaggaca tggtgagtgc cctaccactg agaatactga gacctttatt cgtctgtgtg
      541 agcggtttaa tgaaagaaat ccacctgaac ttataggtgt tcattgtact catggcttca
      601 atcgcactgg tttcctcata tgtgcctttt tggtggagaa aatggattgg agtatcgaag
      661 cagcagttgc tacttttgcc caagccagac caccaggaat ctacaagggt gattatttga
      721 aggaactttt tcgtcggtat ggtgacatag aggaagcacc acccccacct ctattgccag
      781 attggtgttt tgaggatgat gaagacgaag atgaggatga ggatggaaag aaggaatcag
      841 aacccgggtc aagtgcttct tttggcaaaa ggagaaaaga acggttaaaa ctgggcgcta
      901 ttttcttgga aggtgttact gttaaaggtg taactcaagt aacaacacaa ccaaagttag
      961 gagaggtaca gcagaagtgt catcaattct gtggctggga agggtctgga ttccctggag
     1021 cacagcctgt ttccatggac aagcaaaata ttaaactttt agacctgaag ccatacaaag
     1081 taagctggaa agcagatggt actcggtaca tgatgttgat tgatggcaca aatgaagttt
     1141 ttatgattga tagagacaat tcagtatttc atgtttcaaa tctggaattt ccatttcgta
     1201 aagatcttcg tatgcattta tcaaatactc tcttggatgg cgagatgatt attgacagag
     1261 taaatggaca ggctgttcct agatatttga tatatgacat aattaaattc aattcacagc
     1321 ccgttggaga ttgtgatttt aatgttcgtc tgcagtgtat agaacgagaa attataagtc
     1381 ctcgacacga aaaaatgaag actgggctca ttgacaaaac acaggaacca tttagcgtca
     1441 gaaataagcc gttttttgac atctgtactt caagaaagct acttgaagga aattttgcca
     1501 aagaagtgag ccatgaaatg gatggactta tttttcagcc tactggaaaa tacaaacctg
     1561 gtcgatgtga tgatattttg aaatggaagc ctcccagtct gaattctgtg gattttcgtc
     1621 taaaaataac aagaatggga ggagaagggt tacttcctca gaatgttggc ctcctgtatg
     1681 ttggaggtta tgaaagaccc tttgcacaaa tcaaggtgac aaaagagctg aaacagtatg
     1741 acaacaaaat tatagaatgc aaatttgaga acaacagctg ggtcttcatg agacagagaa
     1801 cagacaaaag ttttcctaat gcctacaaca ctgccatggc tgtgtgtaac agcatctcaa
     1861 accctgtcac caaggagatg ctgtttgagt tcatcgacag atgtactgca gcttctcaag
     1921 gacagaagcg aaaacatcat ctggaccctg acacggagct catgccacca ccacctccca
     1981 aaagaccacg ccctttaacc taagacctgc ctgtgacttg agggttaaga agaaagagga
     2041 atgaggaaaa aacgctgttg cccatttttg tagccagaga aattgaaaaa cgactgtggc
     2101 ttgatgttga tacacatttg aatttttttt aaaaaaagaa agttatcgta gccagcctgt
     2161 aaatacttga tgcattcgtt tcctcagtgc tgcaatacat cgtggactta aacatcttat
     2221 ttatctgata aactcagcct taccttgtgg aatctgaaga ttgaaatatc caaaggttta
     2281 aacaagatgg aaatcaatga aaaagaggcc ttgtttatat atgggtagct tcacatttaa
     2341 tttataaagt taacaatctg gaaactgtga gcccataaaa cagtattttt tagaagttgt
     2401 gttacaactg tagcaaattt tccttttaaa aaatccaggc aatggcatta aacattcttg
     2461 ctatcattta ccatggattt cctacatttc tgagatccct gtattcaaaa acaaatgaca
     2521 gggttgttaa gttgtactat attaaacaac ttggtctggt ttatatcatt ttaacaagtt
     2581 ttttggaaat gtaaaagaaa aaagtaaagc tccatttttg tgaattgcat tgtttctaaa
     2641 ggaagtattt tgagtatttt catctgtttg tattgtttcg actatgatat cagatatcca
     2701 gtgtgtccat tctggcagtg gttttgagtt acttcatgga acttggacat acaccaccag
     2761 gtcctttatg tgttgttctc ctgggagact tcttttttta aatgccaggg ctacttctgc
     2821 tttacctcag tggtatcagc acattgtaaa ttacgttaaa acacacaact cactgtgatc
     2881 tatgggagtg atatcaaata caaacgatgt tgtgttcctt ctctcgaaca aaacagcaaa
     2941 gggaatgcca gctaacttct ttgattagaa ggtaatgtca tggaatagaa tgctgtcacc
     3001 agaaaatgct ctcccactgc tagataaatg cagaggcaaa gtgtagacat ctgtaggaac
     3061 taaatatcaa ggaaatcaag acatttattt tcaaagcgag aattctatct acattctatt
     3121 ctgcggatgt tatttctgtt atacatctga attttaaaat gtttactgat agataatgga
     3181 tttaaagggc ggctaattga ttgtctgcca ttgttgataa actgttctaa ttggtttgtg
     3241 aaagaactct gagaattagc taaatggagg ctagtgtatt tatttttaga ggtacatgtt
     3301 ttaagtaaat gtatttaaaa caaattttca tggtctgaat tgtatatgta aatgcatatc
     3361 tgtgtaagta tatgcaagtg tatattcttc tactgattca gtcagacacg aaatccaact
     3421 gaattacccg gtagactgaa tatctgacgg gatttctata tggacatagc acagatgaac
     3481 caaaaatgta tttatattca cctgagtttt aatgttttta aacaaagttt tctcctgcag
     3541 tgcttttcta tatgaaatag ttgagtcaag cttgggcttt tttcttcgtt gttcccaagt
     3601 catgtaatgt atagtgaatt tatccaaaac gccattgtaa cgttttttac tagagtgttt
     3661 ttgcacattt gggcgctaaa gggattaaaa caactgatgc cacaatgact attgaaggag
     3721 ggaaaattct caaggttcca tagtatcttt tacaaaatgg attaaaaaaa aaatgcctat
     3781 gcaacttcaa atagtctgat gcctgcttcc ccaggacgag tgactcttcc acggtgtgct
     3841 gctttccctc cgagcctgtg gaaaggcaga gcccttttgc agatacgaaa gcaagctttt
     3901 gccaatgatc tttaatggag ttaaaatgtt tatggtaatt gtaacctttc aaatgagtta
     3961 cgtgaaaaga gcatctcact tttaatacac ccagatattt tcttcaagtc tttgtgcatt
     4021 ttagcaggac ttaattttct taatttattc tttcctttca attatagtga catagagtag
     4081 ttggccttat aagtagatcc catttagccc ctgaacataa taccatctgc agtattataa
     4141 aagtcattta gaaggtcagg gggataatct agaggcagga ttttggacat tgtgaaggaa
     4201 atgtgctcct tctcagctca cttcaataac tattttctga gactgaagtt ttttagacaa
     4261 gaataagaaa actttgcttt cttcagttat cacatgtgaa agcttttgct ttttgttagg
     4321 aaaaggtgtg gatgactcta ctgcgtggat ggttacattt gctttccgtg aaatgctcag
     4381 aaaatgtcag gacattcctt ttccaattgt gtgtcagtgc gtattgtaat aaaactactg
     4441 atttaaatta gcataagtgt atctactttg ttttactggt gatactcttg tgtcccttct
     4501 attgctggca tcacatccta ctgctgtcag ccacacccag tgtaatccat ctggctttgt
     4561 agagattagc ataaagactt ggaaagcctc tatttttctt tctgagaaac tgagaaattt
     4621 gattgacaca ggggggtgta gtttgaacct tttatttttg aagtggagag gaatgggaga
     4681 aagcacatct ttatatgatg tttatattat ttctcttcat ctaatcttag cagtctactg
     4741 tatgagtaga tattctgtta cattattttt aatgcattta aattttttag aatactcagc
     4801 aatagcaata aatattctga aatga
//
