LOCUS       NP_003791                597 aa            linear   PRI 04-APR-2024
DEFINITION  mRNA-capping enzyme isoform a [Homo sapiens].
ACCESSION   NP_003791
VERSION     NP_003791.3
DBSOURCE    REFSEQ: accession NM_003800.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 597)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (residues 1 to 597)
  AUTHORS   Lombardi,O., Varshney,D., Phillips,N.M. and Cowling,V.H.
  TITLE     c-Myc deregulation induces mRNA capping enzyme dependency
  JOURNAL   Oncotarget 7 (50), 82273-82288 (2016)
   PUBMED   27756891
  REMARK    GeneRIF: c-Myc deregulation induces mRNA capping enzyme dependency
            in tumor cells.
REFERENCE   3  (residues 1 to 597)
  AUTHORS   Mukherjee,C., Bakthavachalu,B. and Schoenberg,D.R.
  TITLE     The cytoplasmic capping complex assembles on adapter protein nck1
            bound to the proline-rich C-terminus of Mammalian capping enzyme
  JOURNAL   PLoS Biol 12 (8), e1001933 (2014)
   PUBMED   25137142
  REMARK    GeneRIF: These results identify a new domain of CE that is specific
            to its function in cytoplasmic capping, and a new role for Nck1 in
            regulating gene expression through its role as the scaffold for
            assembly of the cytoplasmic capping complex.
            Publication Status: Online-Only
REFERENCE   4  (residues 1 to 597)
  AUTHORS   Picard-Jean,F., Bougie,I., Shuto,S. and Bisaillon,M.
  TITLE     The immunosuppressive agent mizoribine monophosphate is an
            inhibitor of the human RNA capping enzyme
  JOURNAL   PLoS One 8 (1), e54621 (2013)
   PUBMED   23349942
  REMARK    GeneRIF: Our biochemical studies provide the first insight that MZP
            can inhibit the formation of the RNA cap structure catalyzed by
            HCE.
REFERENCE   5  (residues 1 to 597)
  AUTHORS   Chu,C., Das,K., Tyminski,J.R., Bauman,J.D., Guan,R., Qiu,W.,
            Montelione,G.T., Arnold,E. and Shatkin,A.J.
  TITLE     Structure of the guanylyltransferase domain of human mRNA capping
            enzyme
  JOURNAL   Proc Natl Acad Sci U S A 108 (25), 10104-10108 (2011)
   PUBMED   21636784
  REMARK    GeneRIF: Data show that human capping enzyme residues 229-567
            comprise the minimum enzymatically active human GTase (hGTase)
            domain and determine the structure by X-ray crystallography.
REFERENCE   6  (residues 1 to 597)
  AUTHORS   Tsukamoto,T., Shibagaki,Y., Niikura,Y. and Mizumoto,K.
  TITLE     Cloning and characterization of three human cDNAs encoding mRNA
            (guanine-7-)-methyltransferase, an mRNA cap methylase
  JOURNAL   Biochem Biophys Res Commun 251 (1), 27-34 (1998)
   PUBMED   9790902
REFERENCE   7  (residues 1 to 597)
  AUTHORS   Pillutla,R.C., Yue,Z., Maldonado,E. and Shatkin,A.J.
  TITLE     Recombinant human mRNA cap methyltransferase binds capping
            enzyme/RNA polymerase IIo complexes
  JOURNAL   J Biol Chem 273 (34), 21443-21446 (1998)
   PUBMED   9705270
REFERENCE   8  (residues 1 to 597)
  AUTHORS   Yamada-Okabe,T., Doi,R., Shimmi,O., Arisawa,M. and Yamada-Okabe,H.
  TITLE     Isolation and characterization of a human cDNA for mRNA 5'-capping
            enzyme
  JOURNAL   Nucleic Acids Res 26 (7), 1700-1706 (1998)
   PUBMED   9512541
REFERENCE   9  (residues 1 to 597)
  AUTHORS   Tsukamoto,T., Shibagaki,Y., Murakoshi,T., Suzuki,M., Nakamura,A.,
            Gotoh,H. and Mizumoto,K.
  TITLE     Cloning and characterization of two human cDNAs encoding the mRNA
            capping enzyme
  JOURNAL   Biochem Biophys Res Commun 243 (1), 101-108 (1998)
   PUBMED   9473487
REFERENCE   10 (residues 1 to 597)
  AUTHORS   Yue,Z., Maldonado,E., Pillutla,R., Cho,H., Reinberg,D. and
            Shatkin,A.J.
  TITLE     Mammalian capping enzyme complements mutant Saccharomyces
            cerevisiae lacking mRNA guanylyltransferase and selectively binds
            the elongating form of RNA polymerase II
  JOURNAL   Proc Natl Acad Sci U S A 94 (24), 12898-12903 (1997)
   PUBMED   9371772
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BP256840.1, AB009022.1, BX537450.1, BC019954.1, AI193199.1 and
            BP396964.1.
            
            On Mar 27, 2007 this sequence version replaced NP_003791.2.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038197.1790820.1, BC019954.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000369485.9/ ENSP00000358497.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q15"
     Protein         1..597
                     /product="mRNA-capping enzyme isoform a"
                     /EC_number="2.7.7.50"
                     /EC_number="3.6.1.74"
                     /note="mRNA-capping enzyme; HCAP1"
                     /calculated_mol_wt=68426
     Region          1..212
                     /region_name="TPase"
                     /note="propagated from UniProtKB/Swiss-Prot (O60942.1)"
     Region          9..175
                     /region_name="Mce1_N"
                     /note="N-terminal triphosphatase domain of mRNA capping
                     enzyme; cd17664"
                     /db_xref="CDD:350502"
     Region          181..220
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (O60942.1)"
     Region          229..597
                     /region_name="GTase"
                     /note="propagated from UniProtKB/Swiss-Prot (O60942.1)"
     Region          248..461
                     /region_name="Adenylation_mRNA_capping"
                     /note="Adenylation domain of GTP-dependent mRNA capping
                     enzymes; cd07895"
                     /db_xref="CDD:185706"
     Site            order(271..273,292,294,299,345,362,413,441,456,458,460)
                     /site_type="active"
                     /db_xref="CDD:185706"
     Site            order(290,358,378..379,381..383,385,409,412,415..416)
                     /site_type="active"
                     /note="CTD docking site [active]"
                     /db_xref="CDD:185706"
     Region          330..386
                     /region_name="Interaction with POLR2A.
                     /evidence=ECO:0000250"
                     /note="propagated from UniProtKB/Swiss-Prot (O60942.1)"
     Region          465..558
                     /region_name="mRNA_cap_C"
                     /note="mRNA capping enzyme, C-terminal domain; pfam03919"
                     /db_xref="CDD:461093"
     Region          570..597
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (O60942.1)"
     CDS             1..597
                     /gene="RNGTT"
                     /gene_synonym="CAP1A; hCAP; HCE; HCE1"
                     /coded_by="NM_003800.5:210..2003"
                     /note="isoform a is encoded by transcript variant 1"
                     /db_xref="CCDS:CCDS5017.1"
                     /db_xref="GeneID:8732"
                     /db_xref="HGNC:HGNC:10073"
                     /db_xref="MIM:603512"
ORIGIN      
        1 mahnkipprw lncprrgqpv agrflplktm lgprydsqva eenrfhpsml snylkslkvk
       61 mgllvdltnt srfydrndie kegikyiklq ckghgecptt entetfirlc erfnernppe
      121 ligvhcthgf nrtgflicaf lvekmdwsie aavatfaqar ppgiykgdyl kelfrrygdi
      181 eeappppllp dwcfeddede dededgkkes epgssasfgk rrkerlklga iflegvtvkg
      241 vtqvttqpkl gevqqkchqf cgwegsgfpg aqpvsmdkqn iklldlkpyk vswkadgtry
      301 mmlidgtnev fmidrdnsvf hvsnlefpfr kdlrmhlsnt lldgemiidr vngqavpryl
      361 iydiikfnsq pvgdcdfnvr lqciereiis prhekmktgl idktqepfsv rnkpffdict
      421 srkllegnfa kevshemdgl ifqptgkykp grcddilkwk ppslnsvdfr lkitrmggeg
      481 llpqnvglly vggyerpfaq ikvtkelkqy dnkiieckfe nnswvfmrqr tdksfpnayn
      541 tamavcnsis npvtkemlfe fidrctaasq gqkrkhhldp dtelmppppp krprplt
//
