LOCUS       NM_003958               5616 bp    mRNA    linear   PRI 01-OCT-2024
DEFINITION  Homo sapiens ring finger protein 8 (RNF8), transcript variant 1,
            mRNA.
ACCESSION   NM_003958
VERSION     NM_003958.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Sun,Q., Sui,Y., Li,S., Zhou,R., Fu,Z., Luo,J. and Zhao,W.
  TITLE     RNF8-mediated multi-ubiquitination of MCM7: Linking disassembly of
            the CMG helicase with DNA damage response in human cells
  JOURNAL   Life Sci 353, 122912 (2024)
   PUBMED   39004272
  REMARK    GeneRIF: RNF8-mediated multi-ubiquitination of MCM7: Linking
            disassembly of the CMG helicase with DNA damage response in human
            cells.
REFERENCE   2  (bases 1 to 5616)
  AUTHORS   Chuah,Y.H., Tay,E.X.Y., Grinchuk,O.V., Yoon,J., Feng,J., Kannan,S.,
            Robert,M., Jakhar,R., Liang,Y., Lee,B.W.L., Wang,L.C., Lim,Y.T.,
            Zhao,T., Sobota,R.M., Lu,G., Low,B.C., Crasta,K.C., Verma,C.S.,
            Lin,Z. and Ong,D.S.T.
  TITLE     CAMK2D serves as a molecular scaffold for RNF8-MAD2 complex to
            induce mitotic checkpoint in glioma
  JOURNAL   Cell Death Differ 30 (8), 1973-1987 (2023)
   PUBMED   37468549
  REMARK    GeneRIF: CAMK2D serves as a molecular scaffold for RNF8-MAD2
            complex to induce mitotic checkpoint in glioma.
REFERENCE   3  (bases 1 to 5616)
  AUTHORS   Kuang,J., Duan,T., Gao,C., Liu,C., Chen,S., Zhu,L.Y., Min,L.,
            Lu,C., Wang,W. and Zhu,L.
  TITLE     RNF8 depletion attenuates hepatocellular carcinoma progression by
            inhibiting epithelial-mesenchymal transition and enhancing drug
            sensitivity
  JOURNAL   Acta Biochim Biophys Sin (Shanghai) 55 (4), 661-671 (2023)
   PUBMED   37154586
  REMARK    GeneRIF: RNF8 depletion attenuates hepatocellular carcinoma
            progression by inhibiting epithelial-mesenchymal transition and
            enhancing drug sensitivity.
REFERENCE   4  (bases 1 to 5616)
  AUTHORS   Zhou,T., Wang,S., Song,X., Liu,W., Dong,F., Huo,Y., Zou,R.,
            Wang,C., Zhang,S., Liu,W., Sun,G., Lin,L., Zeng,K., Dong,X.,
            Guo,Q., Yi,F., Wang,Z., Li,X., Jiang,B., Cao,L. and Zhao,Y.
  TITLE     RNF8 up-regulates AR/ARV7 action to contribute to advanced prostate
            cancer progression
  JOURNAL   Cell Death Dis 13 (4), 352 (2022)
   PUBMED   35428760
  REMARK    GeneRIF: RNF8 up-regulates AR/ARV7 action to contribute to advanced
            prostate cancer progression.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5616)
  AUTHORS   Lu,X., Xu,M., Zhu,Q., Zhang,J., Liu,G., Bao,Y., Gu,L., Tian,Y.,
            Wen,H. and Zhu,W.G.
  TITLE     RNF8-ubiquitinated KMT5A is required for RNF168-induced H2A
            ubiquitination in response to DNA damage
  JOURNAL   FASEB J 35 (4), e21326 (2021)
   PUBMED   33710666
  REMARK    GeneRIF: RNF8-ubiquitinated KMT5A is required for RNF168-induced
            H2A ubiquitination in response to DNA damage.
REFERENCE   6  (bases 1 to 5616)
  AUTHORS   Lehner,B. and Sanderson,C.M.
  TITLE     A protein interaction framework for human mRNA degradation
  JOURNAL   Genome Res 14 (7), 1315-1323 (2004)
   PUBMED   15231747
REFERENCE   7  (bases 1 to 5616)
  AUTHORS   Takano,Y., Adachi,S., Okuno,M., Muto,Y., Yoshioka,T.,
            Matsushima-Nishiwaki,R., Tsurumi,H., Ito,K., Friedman,S.L.,
            Moriwaki,H., Kojima,S. and Okano,Y.
  TITLE     The RING finger protein, RNF8, interacts with retinoid X receptor
            alpha and enhances its transcription-stimulating activity
  JOURNAL   J Biol Chem 279 (18), 18926-18934 (2004)
   PUBMED   14981089
  REMARK    GeneRIF: results suggest a novel function of RNF8 as a regulator of
            RXR alpha-mediated transcriptional activity through interaction
            between their respective N-terminal regions
REFERENCE   8  (bases 1 to 5616)
  AUTHORS   Mungall,A.J., Palmer,S.A., Sims,S.K., Edwards,C.A., Ashurst,J.L.,
            Wilming,L., Jones,M.C., Horton,R., Hunt,S.E., Scott,C.E.,
            Gilbert,J.G., Clamp,M.E., Bethel,G., Milne,S., Ainscough,R.,
            Almeida,J.P., Ambrose,K.D., Andrews,T.D., Ashwell,R.I.,
            Babbage,A.K., Bagguley,C.L., Bailey,J., Banerjee,R., Barker,D.J.,
            Barlow,K.F., Bates,K., Beare,D.M., Beasley,H., Beasley,O.,
            Bird,C.P., Blakey,S., Bray-Allen,S., Brook,J., Brown,A.J.,
            Brown,J.Y., Burford,D.C., Burrill,W., Burton,J., Carder,C.,
            Carter,N.P., Chapman,J.C., Clark,S.Y., Clark,G., Clee,C.M.,
            Clegg,S., Cobley,V., Collier,R.E., Collins,J.E., Colman,L.K.,
            Corby,N.R., Coville,G.J., Culley,K.M., Dhami,P., Davies,J.,
            Dunn,M., Earthrowl,M.E., Ellington,A.E., Evans,K.A., Faulkner,L.,
            Francis,M.D., Frankish,A., Frankland,J., French,L., Garner,P.,
            Garnett,J., Ghori,M.J., Gilby,L.M., Gillson,C.J., Glithero,R.J.,
            Grafham,D.V., Grant,M., Gribble,S., Griffiths,C., Griffiths,M.,
            Hall,R., Halls,K.S., Hammond,S., Harley,J.L., Hart,E.A.,
            Heath,P.D., Heathcott,R., Holmes,S.J., Howden,P.J., Howe,K.L.,
            Howell,G.R., Huckle,E., Humphray,S.J., Humphries,M.D., Hunt,A.R.,
            Johnson,C.M., Joy,A.A., Kay,M., Keenan,S.J., Kimberley,A.M.,
            King,A., Laird,G.K., Langford,C., Lawlor,S., Leongamornlert,D.A.,
            Leversha,M., Lloyd,C.R., Lloyd,D.M., Loveland,J.E., Lovell,J.,
            Martin,S., Mashreghi-Mohammadi,M., Maslen,G.L., Matthews,L.,
            McCann,O.T., McLaren,S.J., McLay,K., McMurray,A., Moore,M.J.,
            Mullikin,J.C., Niblett,D., Nickerson,T., Novik,K.L., Oliver,K.,
            Overton-Larty,E.K., Parker,A., Patel,R., Pearce,A.V., Peck,A.I.,
            Phillimore,B., Phillips,S., Plumb,R.W., Porter,K.M., Ramsey,Y.,
            Ranby,S.A., Rice,C.M., Ross,M.T., Searle,S.M., Sehra,H.K.,
            Sheridan,E., Skuce,C.D., Smith,S., Smith,M., Spraggon,L.,
            Squares,S.L., Steward,C.A., Sycamore,N., Tamlyn-Hall,G., Tester,J.,
            Theaker,A.J., Thomas,D.W., Thorpe,A., Tracey,A., Tromans,A.,
            Tubby,B., Wall,M., Wallis,J.M., West,A.P., White,S.S.,
            Whitehead,S.L., Whittaker,H., Wild,A., Willey,D.J., Wilmer,T.E.,
            Wood,J.M., Wray,P.W., Wyatt,J.C., Young,L., Younger,R.M.,
            Bentley,D.R., Coulson,A., Durbin,R., Hubbard,T., Sulston,J.E.,
            Dunham,I., Rogers,J. and Beck,S.
  TITLE     The DNA sequence and analysis of human chromosome 6
  JOURNAL   Nature 425 (6960), 805-811 (2003)
   PUBMED   14574404
REFERENCE   9  (bases 1 to 5616)
  AUTHORS   Ito,K., Adachi,S., Iwakami,R., Yasuda,H., Muto,Y., Seki,N. and
            Okano,Y.
  TITLE     N-Terminally extended human ubiquitin-conjugating enzymes (E2s)
            mediate the ubiquitination of RING-finger proteins, ARA54 and RNF8
  JOURNAL   Eur J Biochem 268 (9), 2725-2732 (2001)
   PUBMED   11322894
REFERENCE   10 (bases 1 to 5616)
  AUTHORS   Seki,N., Hattori,A., Sugano,S., Suzuki,Y., Nakagawara,A.,
            Ohhira,M., Muramatsu,M., Hori,T. and Saito,T.
  TITLE     Isolation, tissue expression, and chromosomal assignment of a novel
            human gene which encodes a protein with RING finger motif
  JOURNAL   J Hum Genet 43 (4), 272-274 (1998)
   PUBMED   9852682
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA064222.1, AB014546.1 and
            BE327572.1.
            
            On Nov 22, 2018 this sequence version replaced NM_003958.3.
            
            Summary: The protein encoded by this gene contains a RING finger
            motif and an FHA domain. This protein has been shown to interact
            with several class II ubiquitin-conjugating enzymes (E2), including
            UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin
            ligase (E3) in the ubiquitination of certain nuclear proteins. This
            protein is also known to play a role in the DNA damage response and
            depletion of this protein causes cell growth inhibition and cell
            cycle arrest. Alternative splicing results in multiple transcript
            variants. [provided by RefSeq, Feb 2012].
            
            Transcript Variant: This variant (1) represents the most abundant
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.211118.1, AB014546.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000373479.9/ ENSP00000362578.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-70                DA064222.1         12-81
            71-5615             AB014546.1         1-5545
            5616-5616           BE327572.1         13-13               c
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p21.2"
     gene            1..5616
                     /gene="RNF8"
                     /gene_synonym="hRNF8"
                     /note="ring finger protein 8"
                     /db_xref="GeneID:9025"
                     /db_xref="HGNC:HGNC:10071"
                     /db_xref="MIM:611685"
     exon            1..293
                     /gene="RNF8"
                     /gene_synonym="hRNF8"
                     /inference="alignment:Splign:2.1.0"
     CDS             183..1640
                     /gene="RNF8"
                     /gene_synonym="hRNF8"
                     /EC_number="2.3.2.27"
                     /note="isoform 1 is encoded by transcript variant 1;
                     C3HC4-type zinc finger protein; ring finger protein (C3HC4
                     type) 8; E3 ubiquitin-protein ligase RNF8;
                     UBC13/UEV-interacting ring finger protein; ring finger
                     protein 8, E3 ubiquitin protein ligase; RING-type E3
                     ubiquitin transferase RNF8"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase RNF8 isoform 1"
                     /protein_id="NP_003949.1"
                     /db_xref="CCDS:CCDS4834.1"
                     /db_xref="GeneID:9025"
                     /db_xref="HGNC:HGNC:10071"
                     /db_xref="MIM:611685"
                     /translation="MGEPGFFVTGDRAGGRSWCLRRVGMSAGWLLLEDGCEVTVGRGF
                     GVTYQLVSKICPLMISRNHCVLKQNPEGQWTIMDNKSLNGVWLNRARLEPLRVYSIHQ
                     GDYIQLGVPLENKENAEYEYEVTEEDWETIYPCLSPKNDQMIEKNKELRTKRKFSLDE
                     LAGPGAEGPSNLKSKINKVSCESGQPVKSQGKGEVASTPSDNLDPKLTALEPSKTTGA
                     PIYPGFPKVTEVHHEQKASNSSASQRSLQMFKVTMSRILRLKIQMQEKHEAVMNVKKQ
                     TQKGNSKKVVQMEQELQDLQSQLCAEQAQQQARVEQLEKTFQEEEQHLQGLEIAQGEK
                     DLKQQLAQALQEHWALMEELNRSKKDFEAIIQAKNKELEQTKEEKEKMQAQKEEVLSH
                     MNDVLENELQCIICSEYFIEAVTLNCAHSFCSYCINEWMKRKIECPICRKDIKSKTYS
                     LVLDNCINKMVNNLSSEVKERRIVLIRERKAKRLF"
     exon            294..422
                     /gene="RNF8"
                     /gene_synonym="hRNF8"
                     /inference="alignment:Splign:2.1.0"
     exon            423..1157
                     /gene="RNF8"
                     /gene_synonym="hRNF8"
                     /inference="alignment:Splign:2.1.0"
     exon            1158..1220
                     /gene="RNF8"
                     /gene_synonym="hRNF8"
                     /inference="alignment:Splign:2.1.0"
     exon            1221..1310
                     /gene="RNF8"
                     /gene_synonym="hRNF8"
                     /inference="alignment:Splign:2.1.0"
     exon            1311..1418
                     /gene="RNF8"
                     /gene_synonym="hRNF8"
                     /inference="alignment:Splign:2.1.0"
     exon            1419..1623
                     /gene="RNF8"
                     /gene_synonym="hRNF8"
                     /inference="alignment:Splign:2.1.0"
     exon            1624..5616
                     /gene="RNF8"
                     /gene_synonym="hRNF8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2096..2101
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RNF8"
                     /gene_synonym="hRNF8"
                     /note="hexamer: AATAAA"
     polyA_site      2124
                     /gene="RNF8"
                     /gene_synonym="hRNF8"
                     /note="major polyA site"
     regulatory      2914..2919
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RNF8"
                     /gene_synonym="hRNF8"
                     /note="hexamer: AATATA"
     polyA_site      2934
                     /gene="RNF8"
                     /gene_synonym="hRNF8"
     regulatory      4295..4300
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RNF8"
                     /gene_synonym="hRNF8"
                     /note="hexamer: ATTAAA"
     polyA_site      4329
                     /gene="RNF8"
                     /gene_synonym="hRNF8"
     regulatory      5596..5601
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RNF8"
                     /gene_synonym="hRNF8"
                     /note="hexamer: AATAAA"
     polyA_site      5616
                     /gene="RNF8"
                     /gene_synonym="hRNF8"
ORIGIN      
        1 gtagtgctcc tgcttcctgg ccgagggcgg gcgagcggag cctgctttcg cagcgatcgc
       61 gagcgtgtgg cgattgcttc tgtctgttat ttagatatgg aagctgaggg gatgcacaga
      121 ggcagccaga acctaggtca gggtctcgct cggtgctgac cgcccccggg gtcgagtagg
      181 cgatggggga gcccggcttc ttcgtcacag gagaccgcgc cggtggccgg agctggtgcc
      241 tgcggcgggt ggggatgagc gccgggtggc tgctgctgga agatgggtgc gaggtgactg
      301 taggacgagg atttggtgtc acataccaac tggtatcaaa aatctgcccc ctgatgattt
      361 ctcgaaacca ctgtgttttg aagcagaatc ctgagggcca atggacaatt atggacaaca
      421 agagtctaaa tggtgtttgg ctgaacagag cgcgtctgga acctttaagg gtctattcca
      481 ttcatcaggg agactacatc caacttggag tgcctctgga aaataaggag aatgcggagt
      541 atgaatatga agttactgaa gaagactggg agacaatata tccttgtctt tccccaaaga
      601 atgaccaaat gatagaaaaa aataaggaat tgagaactaa aaggaaattc agtttggatg
      661 aattagcagg tcctggagct gaaggcccct caaatttgaa atccaaaata aataaagtgt
      721 cttgtgaatc tggtcagcca gtgaaatcac aggggaaagg tgaagtggcc agtacaccct
      781 ctgacaattt ggatcctaag ttgactgccc ttgagccaag taagaccaca ggggctccca
      841 tttaccctgg cttccccaaa gtcacagagg ttcatcatga gcagaaagcc tcaaactctt
      901 cagcatctca gagaagctta cagatgttta aggtgaccat gtccaggatt ctgaggctca
      961 aaatacagat gcaggaaaaa catgaagccg ttatgaatgt gaaaaagcag acccaaaagg
     1021 ggaactcaaa gaaagttgtg caaatggagc aggaacttca ggacttacag tcccagctgt
     1081 gtgcagagca ggctcagcag caggcaagag tggagcaact agagaagact ttccaggaag
     1141 aggaacagca tcttcagggt ttggagatag cccaaggaga aaaggacctg aagcaacagc
     1201 tggcccaggc tctgcaggag cattgggctc taatggaaga gctaaatcgc agcaagaagg
     1261 actttgaagc aatcattcaa gccaagaaca aagaattaga gcagaccaag gaagagaagg
     1321 agaagatgca agcacagaag gaagaagttc ttagccacat gaatgatgtg ctagagaatg
     1381 agctccaatg tattatttgt tcagaatact tcattgaggc tgtcaccttg aactgtgccc
     1441 acagtttctg ctcctactgt atcaatgaat ggatgaagcg gaagatagaa tgccccattt
     1501 gtcggaagga cattaagtcc aaaacgtact ctttggttct ggacaattgc attaataaga
     1561 tggtaaataa tctgagctca gaagtgaaag aacgacgaat tgttctcatt agggaacgaa
     1621 aagcaaagag attgttctga agaccgtgct ctaagggcat ttgaaagact gccaggtagt
     1681 gcgagcctga gatggtctgg aggattctct ctagccgtga ctccgctgct ctgaaggtca
     1741 actgagaagt cttgtgggac agagacttga gttaggaagc cctcagtcac ttgccttcca
     1801 cggtggccag ccctgctgcc atcattggct gaagcaccac caggattcac ggcacccaac
     1861 tgcttcaggg tacttcgtag actctgcctc actacatgtc gaaagagtta tttgagttct
     1921 cttctgtttt tttttaattt gttgttgttg ttactgtttt gatacctcgg aaacacctcc
     1981 gttgacagtt gttttggata ggttgggtgt accccatggc tgcctctgaa ggcagtgtct
     2041 attttgagag gatggcttac ctcttctttg tgaaaatact atctcatttc ctggaaataa
     2101 aatgtaaaac ctgtcagttg ctcagctggg ctttggtgta tttaccttcc ttcccttcct
     2161 gctcccagac agtctcacaa gtaaacacca gcagctcata gattacaacc aagaaagtga
     2221 ctgtatcaga tgatagactt caagtgaatg tcagcctaag aggccaagct gcagatcgtg
     2281 gcaacagatg agctctgtta acctcctgac tgtcgtgttt cctttttatt acagaggtgg
     2341 gtcactgttt accttgtttc aggggtggga gaaactcctt tccttcagct ggcatttgaa
     2401 tgtttccaaa tctattttat ctgacgtcat gaacacacag gcaatgattt tatgacaact
     2461 tgatgtgctt ttttctttat ttttctttta cattagaagt tttctctctt ttctccacct
     2521 ctcctttatt tttaatttta attttaattt ttgaaacaag atctgactct gttgcccaag
     2581 ctggagtaca gtagtgcaat ctcagctcac tgcaacctcc acctcccaga ctcaagccat
     2641 cctcccacct caacctccca agtagctggg actacaggca tatgccatca cacccagcta
     2701 atttttgttt ttctgtagag acagggtttc accatgttgg ccaggctggt ctcaaattcc
     2761 tgaccttaag tgatccacct gcctaggcct ctaaaatgct gggattacag gcgtgagcca
     2821 ccacgcccag cctatacccc atgtattttc ataaagggtt ggaaactatt gattgccaac
     2881 tttttaagga tggtagggat tttttctttg ttgaatatac tgtgaatttt gggattctgg
     2941 agtccttagt cttcatttcc attcttctgc ccatgattac tgatcaaaat actggtagag
     3001 tcatggagga agtaagttaa atgtggaaaa aatgctttaa aagtacttac tataaccaca
     3061 tcataagaat tctggaaaaa tagagaacaa aaggaaaaga ttgttaccac cttgatttaa
     3121 taacttcttg tttccccata catgtgtttt gtttatagat tgcatgggta tatgtcaatt
     3181 tttatatgtt ctgtgtttag tttacatatt gtaattcatt tttaagagag tacagacata
     3241 cactttttga gtaggcaata tgttcacata gtttgaaatt aaaggtacaa aatggcgtag
     3301 aatgaaatgc ctctcaccct ttccccacag cgactacttc ccactcagag gcaacaagta
     3361 ttacagttgc ttgtatattc ttccagagat ctctgtctat acaagcaaaa acacatgtgg
     3421 tctttttcct tgctttgcac aaatggtaaa ctgtacgcta ttctgaacct tgcttttttc
     3481 agatatatgt tggcaacagt tccatgtcag tacatagttt cctttcttta ttacagctgc
     3541 ctgttttttc attgtgtatt tgcaccatca tttacttttc tggcttactt ttgatagaaa
     3601 tctaggttgg ttcaaacctt tttactctta aaacaatgct gcagttaaca cccttgtaca
     3661 tatatcttta gagagaagat attttaaaaa gaaatacctt tttaaaaatt attttattat
     3721 tttttaatag agactatgtt gcctaggctg gtcttgaaat tctgggctaa agcaatccta
     3781 ccaccttggc ctcccaaagt gctgagatta caggcatgag ccattgcacc tggccaagaa
     3841 gagacatctt gacttgagcc tgaagactat gtacagagac tgacctcaca gactgaccat
     3901 tccatcccac agctgctgga catagagtga tttgcagccc ctcctttcag agtaccacat
     3961 ccctctcaag tgttccacaa catctaggaa agtgagctct taaagacaga ctaaaaagag
     4021 taataaatca atacaacaac attaaaagcc agactagcgg agtttgaatc ttggccctgc
     4081 tgttagtaac tgtgtgagcc ttgggcaagt tactcagcct ccttgtgtct tggttttggt
     4141 ttcttcatct ctaagtaatt atatctgtca ttattgccct gatcacaaac aaaagcctga
     4201 atgtactatg tttaaaagac aaaccaaaaa ttaacccaaa cttgcattat ttgtctgagc
     4261 tacagaatgt tctttccttg gagagatatc tgatattaaa catcatctgc attttacttg
     4321 cctagaaaat acacggtaac ttttctgcct tgcagcatca aactatagta cagctgagcc
     4381 ccagtgctgt gcagtctgac tctaattaaa ggcaccttct ttacagcagg gctctgggga
     4441 actggaaaag ggggtttgtt tcattatctg gttttattaa gcagatgaat gcagccagct
     4501 atatgaagca ctttgcagtg aatggcaggt gtcccatatc tggttatgtt aacctagaaa
     4561 gggctcactc tacctctagg catgtttcat cccaacaatc agactgtgcc aaagcagggg
     4621 actttgtcct ttgtggattg catagctgga tacccatcat ctgtttctct gattggaagc
     4681 tgctgttgta cagaaagacc tgcatttccc ccttgtctcc agttctctca ctactttttc
     4741 ctcctctgtg agtgaccatc caggcagtca ccataactgc tggagtgtct gggattggta
     4801 gctctctcca actgcctgct tgctctttac agcctctctc tgtgactgga atctctccac
     4861 ctcatcgtat ctaaggataa cccagaaaca tggggtgtcc taggtatgtt tatctcgaca
     4921 ctgaaccccc taggcttctg atgaatccag tgattagcta aatttgacat agaaagtaag
     4981 aaggaatgtc tactttgtat tgtggtccta atctaagatc aggagaatcc tggaattgtt
     5041 atctgtctct tgctctgaga tacagacttg ttcataggtg tggggcctga ttggaacagg
     5101 atctgccatt ggtcacaata ggtcaagggc ttgttctgaa ccctagttag cttcattcag
     5161 agaaagaaac ttcctacctg gtcagctttt tcagcttctc caacaaatgg gagttgaggc
     5221 agtaggagtg tggggttctt gggaagacga tggggccttg tattaaggag aaataatgaa
     5281 ctatcttctt gattcttcca ttgagaaaag catatgaatc ctaggaaagg gctcagcctg
     5341 tgataggcat tcaataaata ctccaatgct gtcattcctt tgtctacagc atccccaaac
     5401 aatgtaccag tgatgggcta cctgagctca tctagtcgcc aagcagtatc tcctgcttgc
     5461 tgctgcttta ctacattcca gatgcccacc tcatccaatt tccagagcca ctatctctgc
     5521 tgtccacttt ccttcaggct ctgtgaatac ttcaacctgc tgtgatttgg ggccgtttgt
     5581 actctgcatg tattcaataa attcaattca gcaata
//
