LOCUS       NM_183237               2662 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens ring finger protein 7 (RNF7), transcript variant 3,
            mRNA.
ACCESSION   NM_183237
VERSION     NM_183237.3
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Chang,Y., Chen,Q., Li,H., Xu,J., Tan,M., Xiong,X. and Sun,Y.
  TITLE     The UBE2F-CRL5ASB11-DIRAS2 axis is an oncogene and tumor suppressor
            cascade in pancreatic cancer cells
  JOURNAL   Dev Cell 59 (10), 1317-1332 (2024)
   PUBMED   38574733
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Xu,P., Liu,Y., Liu,C., Guey,B., Li,L., Melenec,P., Ricci,J. and
            Ablasser,A.
  TITLE     The CRL5-SPSB3 ubiquitin ligase targets nuclear cGAS for
            degradation
  JOURNAL   Nature 627 (8005), 873-879 (2024)
   PUBMED   38418882
REFERENCE   3  (bases 1 to 2662)
  AUTHORS   Hua,H., Xie,H., Zheng,J., Lei,L., Deng,Z. and Yu,C.
  TITLE     RNF7 Facilitated the Tumorigenesis of Pancreatic Cancer by
            Activating PI3K/Akt Signaling Pathway
  JOURNAL   Oxid Med Cell Longev 2023, 1728463 (2023)
   PUBMED   36644576
  REMARK    GeneRIF: RNF7 Facilitated the Tumorigenesis of Pancreatic Cancer by
            Activating PI3K/Akt Signaling Pathway.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2662)
  AUTHORS   Xiao,C., Zhang,W., Hua,M., Chen,H., Yang,B., Wang,Y. and Yang,Q.
  TITLE     RNF7 inhibits apoptosis and sunitinib sensitivity and promotes
            glycolysis in renal cell carcinoma via the SOCS1/JAK/STAT3 feedback
            loop
  JOURNAL   Cell Mol Biol Lett 27 (1), 36 (2022)
   PUBMED   35562668
  REMARK    GeneRIF: RNF7 inhibits apoptosis and sunitinib sensitivity and
            promotes glycolysis in renal cell carcinoma via the SOCS1/JAK/STAT3
            feedback loop.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2662)
  AUTHORS   Kostrhon,S., Prabu,J.R., Baek,K., Horn-Ghetko,D., von Gronau,S.,
            Klugel,M., Basquin,J., Alpi,A.F. and Schulman,B.A.
  TITLE     CUL5-ARIH2 E3-E3 ubiquitin ligase structure reveals cullin-specific
            NEDD8 activation
  JOURNAL   Nat Chem Biol 17 (10), 1075-1083 (2021)
   PUBMED   34518685
REFERENCE   6  (bases 1 to 2662)
  AUTHORS   Sun,Y.
  TITLE     Alterations of SAG mRNA in human cancer cell lines: requirement for
            the RING finger domain for apoptosis protection
  JOURNAL   Carcinogenesis 20 (10), 1899-1903 (1999)
   PUBMED   10506102
REFERENCE   7  (bases 1 to 2662)
  AUTHORS   Swaroop,M., Bian,J., Aviram,M., Duan,H., Bisgaier,C.L., Loo,J.A.
            and Sun,Y.
  TITLE     Expression, purification, and biochemical characterization of SAG,
            a ring finger redox-sensitive protein
  JOURNAL   Free Radic Biol Med 27 (1-2), 193-202 (1999)
   PUBMED   10443936
REFERENCE   8  (bases 1 to 2662)
  AUTHORS   Ohta,T., Michel,J.J., Schottelius,A.J. and Xiong,Y.
  TITLE     ROC1, a homolog of APC11, represents a family of cullin partners
            with an associated ubiquitin ligase activity
  JOURNAL   Mol Cell 3 (4), 535-541 (1999)
   PUBMED   10230407
REFERENCE   9  (bases 1 to 2662)
  AUTHORS   Tan,P., Fuchs,S.Y., Chen,A., Wu,K., Gomez,C., Ronai,Z. and Pan,Z.Q.
  TITLE     Recruitment of a ROC1-CUL1 ubiquitin ligase by Skp1 and HOS to
            catalyze the ubiquitination of I kappa B alpha
  JOURNAL   Mol Cell 3 (4), 527-533 (1999)
   PUBMED   10230406
REFERENCE   10 (bases 1 to 2662)
  AUTHORS   Duan,H., Wang,Y., Aviram,M., Swaroop,M., Loo,J.A., Bian,J.,
            Tian,Y., Mueller,T., Bisgaier,C.L. and Sun,Y.
  TITLE     SAG, a novel zinc RING finger protein that protects cells from
            apoptosis induced by redox agents
  JOURNAL   Mol Cell Biol 19 (4), 3145-3155 (1999)
   PUBMED   10082581
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BG742338.1, BG716023.1,
            AC112771.6 and AW150174.1.
            
            On Aug 13, 2020 this sequence version replaced NM_183237.2.
            
            Summary: The protein encoded by this gene is a highly conserved
            ring finger protein. It is an essential subunit of
            SKP1-cullin/CDC53-F box protein ubiquitin ligases, which are a part
            of the protein degradation machinery important for cell cycle
            progression and signal transduction. This protein interacts with,
            and is a substrate of, casein kinase II (CSNK2A1/CKII). The
            phosphorylation of this protein by CSNK2A1 has been shown to
            promote the degradation of IkappaBalpha (CHUK/IKK-alpha/IKBKA) and
            p27Kip1(CDKN1B). Alternatively spliced transcript variants encoding
            distinct isoforms have been reported. [provided by RefSeq, Jul
            2008].
            
            Transcript Variant: This variant (3) uses an alternate splice site
            in the coding region compared to variant 1, which causes a
            frameshift. The resulting shorter isoform (3) has a distinct
            C-terminus, as compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038195.2662981.1, DA488677.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-25                BG742338.1         90-114
            26-405              BG716023.1         61-440
            406-1853            AC112771.6         32467-33914
            1854-1905           AW150174.1         10-61               c
            1906-2662           AC112771.6         33967-34723
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q23"
     gene            1..2662
                     /gene="RNF7"
                     /gene_synonym="CKBBP1; rbx2; ROC2; SAG"
                     /note="ring finger protein 7"
                     /db_xref="GeneID:9616"
                     /db_xref="HGNC:HGNC:10070"
                     /db_xref="MIM:603863"
     exon            1..212
                     /gene="RNF7"
                     /gene_synonym="CKBBP1; rbx2; ROC2; SAG"
                     /inference="alignment:Splign:2.1.0"
     CDS             45..317
                     /gene="RNF7"
                     /gene_synonym="CKBBP1; rbx2; ROC2; SAG"
                     /EC_number="2.3.2.27"
                     /EC_number="2.3.2.32"
                     /note="isoform 3 is encoded by transcript variant 3;
                     sensitive to apoptosis, zinc RING finger protein SAG,
                     regulator of cullins 2; RING-box protein 2; CKII
                     beta-binding protein 1; sensitive to apoptosis zinc RING
                     finger protein SAG; sensitive to apoptosis gene protein"
                     /codon_start=1
                     /product="RING-box protein 2 isoform 3"
                     /protein_id="NP_899060.1"
                     /db_xref="CCDS:CCDS43158.1"
                     /db_xref="GeneID:9616"
                     /db_xref="HGNC:HGNC:10070"
                     /db_xref="MIM:603863"
                     /translation="MADVEDGEETCALASHSGSSGSKSGGDKMFSLKKWNAVAMWSWD
                     VECDTCAICRVQMPVLDVKLKTNKRTVLWSGENVIIPSTTAACPCG"
     exon            213..260
                     /gene="RNF7"
                     /gene_synonym="CKBBP1; rbx2; ROC2; SAG"
                     /inference="alignment:Splign:2.1.0"
     exon            261..2662
                     /gene="RNF7"
                     /gene_synonym="CKBBP1; rbx2; ROC2; SAG"
                     /inference="alignment:Splign:2.1.0"
     regulatory      781..786
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RNF7"
                     /gene_synonym="CKBBP1; rbx2; ROC2; SAG"
                     /note="hexamer: AATAAA"
     polyA_site      804
                     /gene="RNF7"
                     /gene_synonym="CKBBP1; rbx2; ROC2; SAG"
                     /note="major polyA site"
     regulatory      914..919
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RNF7"
                     /gene_synonym="CKBBP1; rbx2; ROC2; SAG"
                     /note="hexamer: AATAAA"
     polyA_site      942
                     /gene="RNF7"
                     /gene_synonym="CKBBP1; rbx2; ROC2; SAG"
     regulatory      1477..1482
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RNF7"
                     /gene_synonym="CKBBP1; rbx2; ROC2; SAG"
                     /note="hexamer: AATAAA"
     polyA_site      1503
                     /gene="RNF7"
                     /gene_synonym="CKBBP1; rbx2; ROC2; SAG"
     regulatory      1547..1552
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RNF7"
                     /gene_synonym="CKBBP1; rbx2; ROC2; SAG"
                     /note="hexamer: AGTAAA"
     polyA_site      1575
                     /gene="RNF7"
                     /gene_synonym="CKBBP1; rbx2; ROC2; SAG"
     regulatory      1607..1612
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RNF7"
                     /gene_synonym="CKBBP1; rbx2; ROC2; SAG"
                     /note="hexamer: AATAAA"
     polyA_site      1635
                     /gene="RNF7"
                     /gene_synonym="CKBBP1; rbx2; ROC2; SAG"
     regulatory      1884..1889
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RNF7"
                     /gene_synonym="CKBBP1; rbx2; ROC2; SAG"
                     /note="hexamer: ATTAAA"
     polyA_site      1905
                     /gene="RNF7"
                     /gene_synonym="CKBBP1; rbx2; ROC2; SAG"
     regulatory      2555..2560
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RNF7"
                     /gene_synonym="CKBBP1; rbx2; ROC2; SAG"
                     /note="hexamer: AGTAAA"
     polyA_site      2574
                     /gene="RNF7"
                     /gene_synonym="CKBBP1; rbx2; ROC2; SAG"
     regulatory      2594..2599
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RNF7"
                     /gene_synonym="CKBBP1; rbx2; ROC2; SAG"
                     /note="hexamer: TATAAA"
     polyA_site      2618
                     /gene="RNF7"
                     /gene_synonym="CKBBP1; rbx2; ROC2; SAG"
     regulatory      2641..2646
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RNF7"
                     /gene_synonym="CKBBP1; rbx2; ROC2; SAG"
                     /note="hexamer: AATATA"
     polyA_site      2662
                     /gene="RNF7"
                     /gene_synonym="CKBBP1; rbx2; ROC2; SAG"
ORIGIN      
        1 cttccccaag ccaacgtctc cgccgtcggc tccgcggcgc cgccatggcc gacgtggaag
       61 acggagagga aacctgcgcc ctggcctctc actccgggag ctcaggctcc aagtcgggag
      121 gcgacaagat gttctccctc aagaagtgga acgcggtggc catgtggagc tgggacgtgg
      181 agtgcgatac gtgcgccatc tgcagggtcc agatgcctgt cttagatgtc aagctgaaaa
      241 caaacaagag gactgtgttg tggtctgggg agaatgtaat cattccttcc acaactgctg
      301 catgtccctg tgggtgaaac agaacaatcg ctgccctctc tgccagcagg actgggtggt
      361 ccaaagaatc ggcaaatgag agtggttaga aggcttctta gcgcagttgt tcagagccct
      421 ggtggatctt gtaatccagt gccctacaaa ggctagaaca ctacagggga tgaattcttc
      481 aaataggagc cgatggatct gtggtccttt gggactcatc aaagccttgg tttagcattt
      541 tgtcagtttt atcttcagaa attctctgcg attaagaaga taatttatta aaggtggtcc
      601 ttcctacctc tgtggtgtgt gtcgcgcaca cagcttagaa gtgctataaa aaaggaaaga
      661 gctccaaatt gaatcacctt tataatttac ccatttctat acaacaggca gtggaagcag
      721 tttcagagaa ctttttgcat gcttatggtt gatcagttaa aaaagaatgt tacagtaaca
      781 aataaagtgc agtttaaaac ccaactctta ctcttaattt gttcctaata cgtatttttg
      841 gcagggagag ggaacggtcc atgaaatctt tatgtgatat aaggatttta agtttgggcc
      901 agtgaacagg gtaaataaaa tttaactttt gagcatatgg aattttgatt gcctttaaag
      961 ttactattct gtatcattga ttcatcaaga aaacctagat ctgcactcac ctcactgttc
     1021 aatcattgag tggtaaagga cagaagtatt ttctagttct ggtcagatga tataattttt
     1081 tttttttttt tttgtggggg tgaagtcttg ctctgccacc caggctggag tgcagtggca
     1141 tgatgtcaac tcactgcaac ctccgcctcc cgggttcaag tgattctcct gcctcagcct
     1201 cccgagtagc tgggattaca ggtgtgtgcc accacacccg gctaattttt gtatttttag
     1261 tagagacggg atttccccat gttggccagg ctggtctcaa actcctgacc tcaggtgatt
     1321 cgcccacctc agcctcccaa agtgctggga ttacaggtgt gggccactgc acctggccca
     1381 gatgatataa tttaactgtg ttttaggtaa catgttaatg agggaaaatg tttaccagtg
     1441 tagcattgga tcaaaaagtt tatcacacag tgttttaata aatggtttat tctgcttacc
     1501 ttatttgaat tgtcattata attttatatg caaccttaat ttgtcaagta aatactttca
     1561 tttaatttga aagaattcat attcatttgc tctttcattt gacaataata aaatttaagg
     1621 tttattacct ggaaagagtg caagaaaata ctaaacaact ttatcaaact gattcacact
     1681 gaggggaggt tattttgtaa caaataatgg accacaagtt cattttgtat tcttctgtag
     1741 tagaggctac tgatatgaat ttaagaaaaa accttagaat cttaactttt cacatctgca
     1801 gatgcagtgt gccattctga atttttcaat ctaagggatg tggatacata gaatgtttaa
     1861 aaagataaca attagaatga aaaattaaaa ttatgtatgc ttttatatgg gttacttttt
     1921 attgatcaca tttctctgga aatttttctt tatatttttt cacagttgtc tttctagcta
     1981 ctgaaaaaat tatacaaata tctttggcca tttaaatgtt tatcattgtg cagtgaactg
     2041 ttttagtaac tttctgtttg acctttttag aaggttgcat ccttaaaatg tgaaccagat
     2101 acatggcttt gcattgaaga ggaaagcagt agaatgtcag gttactttag gcagtgtgac
     2161 agtatataaa tgaaatgtgg aggatggtgg taacaatttg attttccaca cagcaaaacc
     2221 acagtcccgc cacctaacaa agccctgttt atgccacaca ccagcatctg agggaagagg
     2281 aggcactgtg acaaagcagc gctgatgctg ctcagcctgc gatgattaca gctcatctgc
     2341 acagccatct gaaagtttaa tacatactca tcagagggaa catgccggga caattttggg
     2401 ttccaataca aaagttaagc atcaaacagg tttttctttc catttgatta aatgtgctga
     2461 ttttatttta ctgttgcatt agaaacaaac aagttatctt tcgctattta attttccctc
     2521 tatgcaaatt gtgttattac agctgaccgc tatcagtaaa tcttaattct gtcagtccca
     2581 ttgcttcctt ttttataaaa atactgtgtc cccctttact gtcatgaagt gaaattcata
     2641 aatatatttc ctacacatat aa
//
