LOCUS       NM_207111               5777 bp    mRNA    linear   PRI 03-OCT-2024
DEFINITION  Homo sapiens ring finger protein 216 (RNF216), transcript variant
            1, mRNA.
ACCESSION   NM_207111
VERSION     NM_207111.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5777)
  AUTHORS   Xu,W., Chen,K., Yuan,Y., Guo,M., Dong,Q. and Cui,M.
  TITLE     Ring finger protein 216 loss-of-function induces white matter
            hyperintensities by inhibiting oligodendroglia proliferation
  JOURNAL   Cell Biochem Funct 42 (4), e4057 (2024)
   PUBMED   38853469
  REMARK    GeneRIF: Ring finger protein 216 loss-of-function induces white
            matter hyperintensities by inhibiting oligodendroglia
            proliferation.
REFERENCE   2  (bases 1 to 5777)
  AUTHORS   Wu,C. and Zhang,Z.
  TITLE     Clinical and genetic spectrum of RNF216-related disorder: a new
            case and literature review
  JOURNAL   J Med Genet 61 (5), 430-434 (2024)
   PUBMED   38050071
  REMARK    GeneRIF: Clinical and genetic spectrum of RNF216-related disorder:
            a new case and literature review.
            Review article
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5777)
  AUTHORS   Yang,H., Zhu,Y., Li,X., Jiang,Z. and Dai,W.
  TITLE     RNF216 affects the stability of STAU2 in the hypothalamus
  JOURNAL   Dev Growth Differ 65 (7), 408-417 (2023)
   PUBMED   37439148
  REMARK    GeneRIF: RNF216 affects the stability of STAU2 in the hypothalamus.
REFERENCE   4  (bases 1 to 5777)
  AUTHORS   Chen,K.L., Wang,H., Zhao,G.X., Wei,L., Huang,Y.Y., Chen,S.D.,
            Sun,J., Dong,Q., Cui,M. and Yu,J.T.
  TITLE     Whole-Exome Sequencing Identified a Novel Mutation in RNF216 in a
            Family with Gordon Holmes Syndrome
  JOURNAL   J Mol Neurosci 72 (4), 691-694 (2022)
   PUBMED   35088240
  REMARK    GeneRIF: Whole-Exome Sequencing Identified a Novel Mutation in
            RNF216 in a Family with Gordon Holmes Syndrome.
REFERENCE   5  (bases 1 to 5777)
  AUTHORS   Cotton,T.R., Cobbold,S.A., Bernardini,J.P., Richardson,L.W.,
            Wang,X.S. and Lechtenberg,B.C.
  TITLE     Structural basis of K63-ubiquitin chain formation by the
            Gordon-Holmes syndrome RBR E3 ubiquitin ligase RNF216
  JOURNAL   Mol Cell 82 (3), 598-615 (2022)
   PUBMED   34998453
  REMARK    GeneRIF: Structural basis of K63-ubiquitin chain formation by the
            Gordon-Holmes syndrome RBR E3 ubiquitin ligase RNF216.
REFERENCE   6  (bases 1 to 5777)
  AUTHORS   Chen,D., Li,X., Zhai,Z. and Shu,H.B.
  TITLE     A novel zinc finger protein interacts with receptor-interacting
            protein (RIP) and inhibits tumor necrosis factor (TNF)- and
            IL1-induced NF-kappa B activation
  JOURNAL   J Biol Chem 277 (18), 15985-15991 (2002)
   PUBMED   11854271
  REMARK    GeneRIF: interacts with RIP and inhibits tnf and il1-induced NFKB
            activation
REFERENCE   7  (bases 1 to 5777)
  AUTHORS   Simpson,J.C., Wellenreuther,R., Poustka,A., Pepperkok,R. and
            Wiemann,S.
  TITLE     Systematic subcellular localization of novel proteins identified by
            large-scale cDNA sequencing
  JOURNAL   EMBO Rep 1 (3), 287-292 (2000)
   PUBMED   11256614
REFERENCE   8  (bases 1 to 5777)
  AUTHORS   Wang,X. and Stollar,B.D.
  TITLE     Immunoglobulin VH gene expression in human aging
  JOURNAL   Clin Immunol 93 (2), 132-142 (1999)
   PUBMED   10527689
  REMARK    Erratum:[Clin Immunol 2000 Mar;94(3):219]
REFERENCE   9  (bases 1 to 5777)
  AUTHORS   van der Reijden,B.A., Erpelinck-Verschueren,C.A., Lowenberg,B. and
            Jansen,J.H.
  TITLE     TRIADs: a new class of proteins with a novel cysteine-rich
            signature
  JOURNAL   Protein Sci 8 (7), 1557-1561 (1999)
   PUBMED   10422847
REFERENCE   10 (bases 1 to 5777)
  AUTHORS   Perlman,S.
  TITLE     Hereditary Ataxia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301317
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC008167.5 and AC006483.3.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_207111.3.
            
            Summary: This gene encodes a cytoplasmic protein which specifically
            colocalizes and interacts with the serine/threonine protein kinase,
            receptor-interacting protein (RIP). Zinc finger domains of the
            encoded protein are required for its interaction with RIP and for
            inhibition of TNF- and IL1-induced NF-kappa B activation pathways.
            The encoded protein may also function as an E3 ubiquitin-protein
            ligase which accepts ubiquitin from E2 ubiquitin-conjugating
            enzymes and transfers it to substrates. Several alternatively
            spliced transcript variants have been described for this locus but
            the full-length natures of only some are known. [provided by
            RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1, also known as TRIAD3B)
            represents the longer transcript and it encodes the longer isoform
            (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074969.2140652.1,
                                           SRR11853558.21322.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000389902.8/ ENSP00000374552.3
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-123               AC008167.5         59259-59381
            124-259             AC008167.5         79784-79919
            260-393             AC008167.5         87943-88076
            394-1236            AC008167.5         99107-99949
            1237-1313           AC008167.5         101570-101646
            1314-1416           AC008167.5         110105-110207
            1417-1581           AC008167.5         111326-111490
            1582-1696           AC008167.5         115484-115598
            1697-1836           AC008167.5         119750-119889
            1837-1887           AC008167.5         124156-124206
            1888-2025           AC008167.5         125732-125869
            2026-2174           AC008167.5         128059-128207
            2175-2253           AC008167.5         129083-129161
            2254-2351           AC006483.3         48049-48146
            2352-2574           AC006483.3         59183-59405
            2575-2644           AC006483.3         76434-76503
            2645-5777           AC006483.3         77380-80512
FEATURES             Location/Qualifiers
     source          1..5777
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p22.1"
     gene            1..5777
                     /gene="RNF216"
                     /gene_synonym="CAHH; TRIAD3; U7I1; UBCE7IP1; ZIN"
                     /note="ring finger protein 216"
                     /db_xref="GeneID:54476"
                     /db_xref="HGNC:HGNC:21698"
                     /db_xref="MIM:609948"
     exon            1..123
                     /gene="RNF216"
                     /gene_synonym="CAHH; TRIAD3; U7I1; UBCE7IP1; ZIN"
                     /inference="alignment:Splign:2.1.0"
     exon            124..259
                     /gene="RNF216"
                     /gene_synonym="CAHH; TRIAD3; U7I1; UBCE7IP1; ZIN"
                     /inference="alignment:Splign:2.1.0"
     CDS             193..2964
                     /gene="RNF216"
                     /gene_synonym="CAHH; TRIAD3; U7I1; UBCE7IP1; ZIN"
                     /EC_number="2.3.2.27"
                     /note="isoform a is encoded by transcript variant 1; E3
                     ubiquitin-protein ligase RNF216; triad domain-containing
                     protein 3; zinc finger protein inhibiting NF-kappa-B;
                     ubiquitin-conjugating enzyme 7-interacting protein 1;
                     RING-type E3 ubiquitin transferase RNF216"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase RNF216 isoform a"
                     /protein_id="NP_996994.1"
                     /db_xref="CCDS:CCDS34594.1"
                     /db_xref="GeneID:54476"
                     /db_xref="HGNC:HGNC:21698"
                     /db_xref="MIM:609948"
                     /translation="MEEGNNNEEVIHLNNFHCHRGQEWINLRDGPITISDSSDEERIP
                     MLVTPAPQQHEEEDLDDDVILTETNKPQRSRPNLIKPAAQWQDLKRLGEERPKKSRAA
                     FESDKSSYFSVCNNPLFDSGAQDDSEDDYGEFLDLGPPGISEFTKPSGQTEREPKPGP
                     SHNQAANDIVNPRSEQKVIILEEGSLLYTESDPLETQNQSSEDSETELLSNLGESAAL
                     ADDQAIEEDCWLDHPYFQSLNQQPREITNQVVPQERQPEAELGRLLFQHEFPGPAFPR
                     PEPQQGGISGPSSPQPAHPLGEFEDQQLASDDEEPGPAFPMQESQEPNLENIWGQEAA
                     EVDQELVELLVKETEARFPDVANGFIEEIIHFKNYYDLNVLCNFLLENPDYPKREDRI
                     IINPSSSLLASQDETKLPKIDFFDYSKLTPLDQRCFIQAADLLMADFKVLSSQDIKWA
                     LHELKGHYAITRKALSDAIKKWQELSPETSGKRKKRKQMNQYSYIDFKFEQGDIKIEK
                     RMFFLENKRRHCRSYDRRALLPAVQQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKD
                     GQLIECRCCYGEFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKLELSCMEGSCTCS
                     FPTSELEKVLPQTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPN
                     PHCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTSIEEKMTAARIRKCHKCGTGLI
                     KSEGCNRMSCRCGAQMCYLCRVSINGYDHFCQHPRSPGAPCQECSRCSLWTDPTEDDE
                     KLIEEIQKEAEEEQKRKNGENTFKRIGPPLEKPVEKVQRVEALPRPVPQNLPQPQMPP
                     YAFAHPPFPLPPVRPVFNNFPLNMGPIPAPYVPPLPNVRVNYDFGPIHMPLEHNLPMH
                     FGPQPRHRF"
     exon            260..393
                     /gene="RNF216"
                     /gene_synonym="CAHH; TRIAD3; U7I1; UBCE7IP1; ZIN"
                     /inference="alignment:Splign:2.1.0"
     exon            394..1236
                     /gene="RNF216"
                     /gene_synonym="CAHH; TRIAD3; U7I1; UBCE7IP1; ZIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1237..1313
                     /gene="RNF216"
                     /gene_synonym="CAHH; TRIAD3; U7I1; UBCE7IP1; ZIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1314..1416
                     /gene="RNF216"
                     /gene_synonym="CAHH; TRIAD3; U7I1; UBCE7IP1; ZIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1417..1581
                     /gene="RNF216"
                     /gene_synonym="CAHH; TRIAD3; U7I1; UBCE7IP1; ZIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1582..1696
                     /gene="RNF216"
                     /gene_synonym="CAHH; TRIAD3; U7I1; UBCE7IP1; ZIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1697..1836
                     /gene="RNF216"
                     /gene_synonym="CAHH; TRIAD3; U7I1; UBCE7IP1; ZIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1837..1887
                     /gene="RNF216"
                     /gene_synonym="CAHH; TRIAD3; U7I1; UBCE7IP1; ZIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1888..2025
                     /gene="RNF216"
                     /gene_synonym="CAHH; TRIAD3; U7I1; UBCE7IP1; ZIN"
                     /inference="alignment:Splign:2.1.0"
     exon            2026..2174
                     /gene="RNF216"
                     /gene_synonym="CAHH; TRIAD3; U7I1; UBCE7IP1; ZIN"
                     /inference="alignment:Splign:2.1.0"
     exon            2175..2253
                     /gene="RNF216"
                     /gene_synonym="CAHH; TRIAD3; U7I1; UBCE7IP1; ZIN"
                     /inference="alignment:Splign:2.1.0"
     exon            2254..2351
                     /gene="RNF216"
                     /gene_synonym="CAHH; TRIAD3; U7I1; UBCE7IP1; ZIN"
                     /inference="alignment:Splign:2.1.0"
     exon            2352..2574
                     /gene="RNF216"
                     /gene_synonym="CAHH; TRIAD3; U7I1; UBCE7IP1; ZIN"
                     /inference="alignment:Splign:2.1.0"
     exon            2575..2644
                     /gene="RNF216"
                     /gene_synonym="CAHH; TRIAD3; U7I1; UBCE7IP1; ZIN"
                     /inference="alignment:Splign:2.1.0"
     exon            2645..5777
                     /gene="RNF216"
                     /gene_synonym="CAHH; TRIAD3; U7I1; UBCE7IP1; ZIN"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3146..3151
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RNF216"
                     /gene_synonym="CAHH; TRIAD3; U7I1; UBCE7IP1; ZIN"
                     /note="hexamer: AATAAA"
     polyA_site      3169
                     /gene="RNF216"
                     /gene_synonym="CAHH; TRIAD3; U7I1; UBCE7IP1; ZIN"
                     /note="major polyA site"
     regulatory      5754..5759
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RNF216"
                     /gene_synonym="CAHH; TRIAD3; U7I1; UBCE7IP1; ZIN"
                     /note="hexamer: AATAAA"
     polyA_site      5777
                     /gene="RNF216"
                     /gene_synonym="CAHH; TRIAD3; U7I1; UBCE7IP1; ZIN"
                     /note="major polyA site"
ORIGIN      
        1 gcggcagggc cggaaggagt gcagcggctg ccacggagct cgcagctgcg gcttctgagg
       61 agtaagcggc gcggtagcga aggccgccga acctgcctgg ctagccggcg acttgagtga
      121 cgattctttt gaaacagatg gtcaccatgt ttagatatta gcagtcccat atatgcatgt
      181 ctgcatttga aaatggaaga gggaaacaac aatgaagagg taattcactt gaacaacttt
      241 cactgccatc ggggacaaga gtggatcaat ctccgagatg ggcccatcac catatctgac
      301 tcctcagatg aggaaaggat tccaatgctg gtcaccccag ctcctcagca gcatgaagaa
      361 gaggacctgg atgatgatgt catcctgaca gaaacaaata aacctcagag atcacgaccc
      421 aatctcatca aaccagctgc ccagtggcaa gatctgaaaa ggttgggaga agaaaggcct
      481 aaaaagtcta gagcagcatt tgaatcagat aagagcagct atttttcagt gtgtaacaac
      541 ccattgtttg attctggggc acaggatgat tctgaggatg actacggtga atttctggat
      601 cttgggcctc ctggaatctc tgaattcact aagccaagtg gccaaacaga aagagaaccc
      661 aagcctggac cgagtcataa ccaagcagca aatgacattg tcaaccccag atcagagcag
      721 aaagtcatca tcttggaaga aggtagcctt ctttacacag aaagcgatcc tttggaaact
      781 cagaaccagt catccgaaga ctcagagaca gagctgttat caaatctagg agagtcagct
      841 gctctagcag atgatcaggc catcgaagaa gactgctggt tagatcatcc ttacttccag
      901 tctctgaacc aacagccccg tgaaataaca aaccaggtcg ttcctcagga acggcagcct
      961 gaagcagaac tgggccgctt gttgtttcag catgaattcc cagggcccgc ttttccaagg
     1021 ccggaacccc agcaaggtgg gatttcaggc ccctcttctc ctcagcctgc ccatcctcta
     1081 ggagagtttg aagaccagca gttagcaagt gatgatgaag agccaggtcc agcctttcca
     1141 atgcaagaat ctcaagagcc caatttggaa aacatttggg ggcaagaagc tgcagaggta
     1201 gatcaagagc tcgttgaact actagtgaaa gaaacggaag caagatttcc agatgtagca
     1261 aatgggttta ttgaggaaat aattcatttt aagaattatt atgatctgaa tgtactttgt
     1321 aattttcttc tggaaaaccc agattatcca aagagagaag acagaatcat tataaatccc
     1381 agtagcagtc tgctggccag ccaagatgag acaaagttgc ctaaaataga cttttttgac
     1441 tattctaaat tgacccctct tgaccagcgc tgcttcatcc aagctgctga cctcctcatg
     1501 gccgacttca aagtgctcag tagtcaggac atcaagtggg ccctgcacga gctcaaagga
     1561 cactatgcaa tcacccgaaa ggccttgtct gatgccatta aaaaatggca ggagctgtca
     1621 ccagaaacca gtggaaaaag gaagaagaga aaacaaatga accagtattc ttacattgat
     1681 ttcaagtttg aacaaggtga cataaaaata gaaaagagga tgttctttct tgaaaataag
     1741 cgacgacatt gtaggtccta tgaccgacgt gctctccttc cagctgtgca acaagagcag
     1801 gagttctatg agcagaaaat caaagagatg gcagagcatg aagacttttt gcttgcccta
     1861 cagatgaatg aagaacagta tcaaaaggat ggccagctga ttgagtgtcg ctgctgctat
     1921 ggggaatttc cattcgagga gctgacgcag tgcgcagatg ctcacttgtt ctgcaaagag
     1981 tgtctcatca gatatgccca agaggcagtc tttggatctg gaaagttgga gctcagctgc
     2041 atggaaggca gctgcacgtg ttcgttccca accagtgagc tggagaaggt gctcccccag
     2101 accatcctgt ataagtacta tgagcgaaaa gccgaggagg aggttgcggc agcctacgcc
     2161 gacgagcttg tcaggtgccc gtcctgtagc tttccggctc tgttggacag tgatgtgaag
     2221 aggttcagct gtcctaatcc tcactgccga aaggaaacct gtaggaagtg tcagggactc
     2281 tggaaagaac ataatggcct cacctgtgaa gagctggctg aaaaagacga catcaagtac
     2341 cgtacctcta ttgaagaaaa aatgactgct gcccgcatta gaaaatgcca caagtgtggg
     2401 actggcctca tcaaatctga aggctgcaac cgcatgtctt gccgctgtgg tgcccagatg
     2461 tgctacctct gtcgagtttc tattaatgga tatgaccatt tctgccaaca tccccgctca
     2521 ccaggagccc cttgccagga gtgttcaaga tgctctctct ggaccgatcc cactgaagat
     2581 gatgagaagc ttattgagga aatccagaag gaggctgaag aggaacagaa aagaaagaat
     2641 ggagagaaca ccttcaaacg cattggaccc ccgctggaga agcctgtgga gaaggtgcag
     2701 agggtggagg ccctcccgag gcccgttccg cagaacctgc cacagccaca gatgccaccc
     2761 tatgccttcg cgcacccacc cttccccctg cctcccgtgc ggcctgtgtt caacaacttc
     2821 ccactcaaca tggggcctat cccagccccg tacgtgcccc ctctgcccaa cgtgcgggtc
     2881 aactatgact tcggtcccat ccacatgccc ctggagcaca acctgcccat gcactttggc
     2941 ccccagccgc ggcatcgctt ctgatggccc cgaatcccca ttgagcagca caaagcccgt
     3001 ttggggtagg agtgtggatg gagaaccctc ccccaaggct ggtgtctgta ccattgcatc
     3061 ctaagtcagc ttgaagggta ggctggtttt cttcccaccc ctttcctaga agggctactg
     3121 ctcctggaag agtggacgga tccataataa agacgtccca aatggtggag ttcggagaga
     3181 gctgcgatgt gaactgcccc tcccctcgca tcccccaggc caccaacggc agtccttctg
     3241 ccttgtccat ggcataggcc atagaccagg tccctgctgc tcacacctgg gcctctcctc
     3301 ggagccgacc cctgggtagc aaggcagccg agagcatctc cctggagggg cccacggttg
     3361 ggccaagggc agagggggct gcacctgcgg gcctgggaag cattgctcag ggtggggggc
     3421 tgggaccatg gcccgcagag gcactgccac agctgtgagg gccaagatgc tgtcccccca
     3481 tccaaaaccc gtgcgccact gcagtgagtg ttgagggcac ctctcctccc ctcttacacc
     3541 tactcagatg aggcagcagc agacccatct cgcggcgggg gttttgttct gttgccgcct
     3601 aactttctca tcctcggtct ctggaaagtc aggctgagaa atcctttccc aggccaggcc
     3661 gctgcggtac actggatggt tctgaagctg gcccattgaa agagcctctt aaggcagctg
     3721 ggacagaggc ctggtggccc tgctgggcag cccaactgct gggggagacg tttctgccac
     3781 cctgggtgat gagcagcttt tcccccctgg ctttctgggg gaggagtggg cctccttagg
     3841 gagacaggtg accctgggtg ccacccctgc cccgtgtgtg ccccgggtgt tctcagtggt
     3901 tgctgaaggc aggtagaggg tgctgtccag tatcccccat gtgaaggtca cttcccttct
     3961 catggagtca gctgagcatc agctcagccc tgccatgtcc ccactcaccc tcctcgcctc
     4021 ctgtccggcc ctgggtttct agcggtgcct gaggcatcac tctggcccat tgacagatga
     4081 gaggtctgaa gccttcctgg ccacaggcat cactttctcc tcctcctcat gccctgcctt
     4141 gtccttgtcg tgttgccatg gggttctgag aggctgggag ttcacagacc tcagacacag
     4201 ctgagtccga caaccattgg ggtggggctg catcagtctc cggagtggcc cgccacctcc
     4261 tgaagcaggg cctggcccac ccaaggtgcc tggggcaggc gggcaccgtc attcgctgcc
     4321 attggcttct cagatgtatt tcaaggacta aagtgggctc taagatctaa gatggcccgg
     4381 cgcggtggct cccgcctgta atcccagcac tttgggaggc cgaggcgggc ggatgagttg
     4441 aggtcgggag tttgagtccc cgtctctact aaaaatacaa aattagccgg acaaggtggc
     4501 gcatgcctat aatcccaggt actcaggagg ctgaggcagg agaatcactt gaacctggga
     4561 ggcagaggtt gcagtgagcc aagattgtgc cactgcactc cagcctgagc aacaaaagca
     4621 aaactctatc tttaaaaaaa aaaaaaaaaa aactaagata cccattcttt ctgcctttgc
     4681 ccaggatgga ggctttgaag acaggtggtc acctggacct ggggtaaggg tgcgcaggga
     4741 ggccgggctt gggggcagcc tgctgctatg tccttgctgg cgcccatacc tgttggactg
     4801 cctgaacttt gctactagcc tgaacgacag tgccttggaa aggcaggctc catgcggcag
     4861 caacaggatt gtcttcggta ccttaaatat gctccctgtg cttccccagg gaggatctct
     4921 gggagcagag ggcggctgta gacatcacag ctgaagaggt gacctcaggc cctgggagcc
     4981 tcagctccct ctgccctgta ccctaggcac gtggctccag caagagcccc aggcaggaca
     5041 aagggagcag gcagcagctg cagaaacagc cctagacagg tgctggcctc aaccctggca
     5101 ggggaggggg agcccagagt gatgtttctc ctaaggccaa accccagggg tgggtcattt
     5161 gagaggagtt ccagctgttc tggccacact gggctggtgg ggaaggagcg gcggcctctt
     5221 tccttggagg gtccctgatt cctgaggatc ctccccgagc caggcacggg ggaccacagg
     5281 gctgccgtga gcacccaccc tggttctcca ggcccctctg tccagaggca gggcctgaac
     5341 ctcgggctcc tgcctggacc agtgacagca ttggggagag aagcactggg ggtcttcaga
     5401 ccagccagca aggggccgtg ccagtgccca gagagcaggt cagacaacag gcaaccctga
     5461 ggcagctggc tccctctctc tgccctgcag ggcttgccgc agggcgtgag gctccccagc
     5521 cacagccccc tcttacttgc cttaaaactg gagagaggaa tctagtattt gcacttagca
     5581 aaatatttaa aaactgaaaa aaatgtgcat gatctgtcac tttgtataaa aacaagaaac
     5641 gattagttca ttaaactttt tctttacctt ctggtagtaa aaatagataa aataattccg
     5701 tttcaactac tgtgtgtaaa aagcctgtat catatgtaac tgggactttt gtaaataaat
     5761 gtttgtgcgc tctgctc
//
