LOCUS       NM_152267               3182 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens ring finger protein 185 (RNF185), transcript variant
            1, mRNA.
ACCESSION   NM_152267
VERSION     NM_152267.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Van Espen,B., Oo,H.Z., Collins,C., Fazli,L., Molinolo,A., Yip,K.,
            Murad,R., Gleave,M. and Ronai,Z.A.
  TITLE     RNF185 Control of COL3A1 Expression Limits Prostate Cancer
            Migration and Metastatic Potential
  JOURNAL   Mol Cancer Res 22 (1), 41-54 (2024)
   PUBMED   37831068
  REMARK    GeneRIF: RNF185 Control of COL3A1 Expression Limits Prostate Cancer
            Migration and Metastatic Potential.
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Lin,K., Shen,S.H., Lu,F., Zheng,P., Wu,S., Liao,J., Jiang,X.,
            Zeng,G. and Wei,D.
  TITLE     CRISPR screening of E3 ubiquitin ligases reveals Ring Finger
            Protein 185 as a novel tumor suppressor in glioblastoma repressed
            by promoter hypermethylation and miR-587
  JOURNAL   J Transl Med 20 (1), 96 (2022)
   PUBMED   35183197
  REMARK    GeneRIF: CRISPR screening of E3 ubiquitin ligases reveals Ring
            Finger Protein 185 as a novel tumor suppressor in glioblastoma
            repressed by promoter hypermethylation and miR-587.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3182)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 3182)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 3182)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3182)
  AUTHORS   Zhou,Y., Shang,H., Zhang,C., Liu,Y., Zhao,Y., Shuang,F., Zhong,H.,
            Tang,J. and Hou,S.
  TITLE     The E3 ligase RNF185 negatively regulates osteogenic
            differentiation by targeting Dvl2 for degradation
  JOURNAL   Biochem Biophys Res Commun 447 (3), 431-436 (2014)
   PUBMED   24727453
  REMARK    GeneRIF: these results indicate that RNF185 negatively regulates
            osteogenesis through the degradation of Dvl2 and down-regulation of
            canonical Wnt signaling pathway and suggest a possible therapeutic
            target in osteoporosis.
REFERENCE   7  (bases 1 to 3182)
  AUTHORS   El Khouri,E., Le Pavec,G., Toledano,M.B. and Delaunay-Moisan,A.
  TITLE     RNF185 is a novel E3 ligase of endoplasmic reticulum-associated
            degradation (ERAD) that targets cystic fibrosis transmembrane
            conductance regulator (CFTR)
  JOURNAL   J Biol Chem 288 (43), 31177-31191 (2013)
   PUBMED   24019521
  REMARK    GeneRIF: data thus identify RNF185 and RNF5 as a novel E3 ligase
            module that is central to the control of CFTR degradation.
REFERENCE   8  (bases 1 to 3182)
  AUTHORS   Tang,F., Wang,B., Li,N., Wu,Y., Jia,J., Suo,T., Chen,Q., Liu,Y.J.
            and Tang,J.
  TITLE     RNF185, a novel mitochondrial ubiquitin E3 ligase, regulates
            autophagy through interaction with BNIP1
  JOURNAL   PLoS One 6 (9), e24367 (2011)
   PUBMED   21931693
  REMARK    GeneRIF: Human BNIP1 colocalizes with RNF185 at mitochondria and is
            polyubiquitinated by RNF185 through K63-based ubiquitin linkage in
            vivo.
REFERENCE   9  (bases 1 to 3182)
  AUTHORS   Markson,G., Kiel,C., Hyde,R., Brown,S., Charalabous,P., Bremm,A.,
            Semple,J., Woodsmith,J., Duley,S., Salehi-Ashtiani,K., Vidal,M.,
            Komander,D., Serrano,L., Lehner,P. and Sanderson,C.M.
  TITLE     Analysis of the human E2 ubiquitin conjugating enzyme protein
            interaction network
  JOURNAL   Genome Res 19 (10), 1905-1911 (2009)
   PUBMED   19549727
REFERENCE   10 (bases 1 to 3182)
  AUTHORS   Collins,J.E., Wright,C.L., Edwards,C.A., Davis,M.P., Grinham,J.A.,
            Cole,C.G., Goward,M.E., Aguado,B., Mallya,M., Mokrab,Y.,
            Huckle,E.J., Beare,D.M. and Dunham,I.
  TITLE     A genome annotation-driven approach to cloning the human ORFeome
  JOURNAL   Genome Biol 5 (10), R84 (2004)
   PUBMED   15461802
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DB134817.1, BC033166.1 and AA258379.1.
            
            On Nov 23, 2018 this sequence version replaced NM_152267.3.
            
            Transcript Variant: This variant (1) represents the predominant
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.215631.1,
                                           SRR1803611.272208.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000326132.11/ ENSP00000320508.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-299               DB134817.1         31-329
            300-2977            BC033166.1         265-2942
            2978-3182           AA258379.1         7-211               c
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q12.2"
     gene            1..3182
                     /gene="RNF185"
                     /note="ring finger protein 185"
                     /db_xref="GeneID:91445"
                     /db_xref="HGNC:HGNC:26783"
                     /db_xref="MIM:620096"
     exon            1..122
                     /gene="RNF185"
                     /inference="alignment:Splign:2.1.0"
     exon            123..346
                     /gene="RNF185"
                     /inference="alignment:Splign:2.1.0"
     CDS             171..749
                     /gene="RNF185"
                     /EC_number="2.3.2.27"
                     /note="isoform 1 is encoded by transcript variant 1;
                     BSK65-MONO1; BSK65-PANC1; BSK65-TEST3; BSK65-PANC2;
                     BSK65-TEST2; BSK65-MONO2; BSK65-TEST1; E3
                     ubiquitin-protein ligase RNF185; RING-type E3 ubiquitin
                     transferase RNF185"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase RNF185 isoform 1"
                     /protein_id="NP_689480.2"
                     /db_xref="CCDS:CCDS13890.1"
                     /db_xref="GeneID:91445"
                     /db_xref="HGNC:HGNC:26783"
                     /db_xref="MIM:620096"
                     /translation="MASKGPSASASPENSSAGGPSGSSNGAGESGGQDSTFECNICLD
                     TAKDAVISLCGHLFCWPCLHQWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDP
                     REKTPPRPQGQRPEPENRGGFQGFGFGDGGFQMSFGIGAFPFGIFATAFNINDGRPPP
                     AVPGTPQYVDEQFLSRLFLFVALVIMFWLLIA"
     exon            347..365
                     /gene="RNF185"
                     /inference="alignment:Splign:2.1.0"
     exon            366..478
                     /gene="RNF185"
                     /inference="alignment:Splign:2.1.0"
     exon            479..533
                     /gene="RNF185"
                     /inference="alignment:Splign:2.1.0"
     exon            534..651
                     /gene="RNF185"
                     /inference="alignment:Splign:2.1.0"
     exon            652..3182
                     /gene="RNF185"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3158..3163
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RNF185"
                     /note="hexamer: AACAAA"
     polyA_site      3182
                     /gene="RNF185"
                     /note="major polyA site"
ORIGIN      
        1 atgtgactgg agtccgcgta ggaggggtcg gaggtcttac ccaacagatt gacgcggcgt
       61 tagtattggc cgtgtacccg aaaaactgat tgactgggct ggcgttaact gtgcggaggc
      121 aggatttccc tggggaaagt cttcactcag agcttcgctg acagccaagg atggcaagca
      181 aggggccctc ggcctctgca tctcctgaga actccagtgc aggggggccc agtgggagca
      241 gcaatggcgc tggcgagagc ggagggcagg acagcacttt cgagtgcaac atctgcttgg
      301 acacagccaa ggatgccgtc atcagcctgt gtggccacct cttctgttgg ccgtgtttac
      361 atcagtggtt ggagaccaga cctaacagac aggtgtgtcc tgtttgcaaa gctggcatca
      421 gccgagacaa ggtcatcccc ctctatggaa ggggcagcac tgggcaacag gaccccagag
      481 agaagacccc tcctcgtcct caaggacaga ggccagagcc ggagaataga gggggatttc
      541 aaggatttgg atttggagat ggtggcttcc agatgtcttt tggaattggg gcatttccct
      601 ttgggatatt tgccacagca tttaatataa atgatgggcg gcctcctcca gctgtccctg
      661 ggacacccca gtatgtggac gagcagttcc tgtcacgcct cttcctattt gtggccctgg
      721 tgatcatgtt ctggctcctg attgcctaat gctgggctcc tgcctacatc cgtggcaggg
      781 ctctggactg gtgacgtgcc accccaactc ctggtgtttg gcttcctggc taatcttgac
      841 tcctggaatc agtgggatca gtaacacatc aaggagtctt gtttcttcat cagagctttg
      901 gaactcgaga ccagttggcg atgacccctg aatatcgcca ccgctgtaaa cactctataa
      961 cttcaggcct tggcattgag tcatctctca tgggtgacac catgaaatct tgtttcagcc
     1021 agttctgcag gtcctgactc tgcagaggga agaggcagaa agagagaaac tgtcagagta
     1081 taatttcacc tgagtttaat attacagaaa caaagggatg caccaaatgg tatttctgga
     1141 aattttcatg tctttaaata ccccttggta agttgcttct gaagccagtg ggggctcctc
     1201 agatagagag gttccccttt caaatcccag tgccgctctg ttctctttcc ttcccctccc
     1261 actccccctc ttcttcctct gtagagatgc aagaaattgc tgtcccataa aaatcataat
     1321 tgcagtagct aaagctgggg tcacttcgtg aattcaccag agactcaaag atcttttatt
     1381 ggctctgggc tgtgctcagt gtctttggcc tcagagaaca acttgaatga cttcctggtt
     1441 tcctggcata aattattcct ggtgagacat gtggcttaac tcacaggttt cccatcagct
     1501 ttctccctaa aactatgttc atctgcctct ctctgccaga gaacatacag ccgagaatac
     1561 tgccgaagct gagactgact actgtgcatt aggaaagacc tggagtcagg actttggtgg
     1621 gatttggagc tccgaggcag taataactga acaagcagcc ctgtccccta ggctgcagaa
     1681 gcttgaatgc atcctctccc agaacctgcc acaggaaact gggggctttg tcaggtcagc
     1741 ccaactgcat gcaaaagacc accatcctca gaagccaagt tgtcttttat gaagaggcaa
     1801 ggaaagggga aacccacatg tgaccctgat tttggtatgg cttgatagag ttccctgaaa
     1861 actccttgta tgtgtgctaa aaccagggaa gcatgtgact gccaagcagg caacccctga
     1921 tgatttgtaa agccaggtgg cagggccttg gggagcccca gcacaatgat attgtgtggt
     1981 cttccctcct gtggaatcga ggggaaatta ttcttcccaa taccttgatt tgattttcag
     2041 tttcataagc ttcttcctct gaatcttatt gagggactat ggtaccaagc aggtaggact
     2101 gttcacctgg tggaacagtt cttgctctgc cttctaggct tcatcccaga aatccagcct
     2161 ctttctggag accccaaagc tggagggaga tgggctttcc tctgggcctc tctcctactt
     2221 tgccatccac actgctcctg gctaacccca gcaataacca acaaatggta ggaagcccca
     2281 tctattgctt tttctcaatt atgactgcat agtttatgga aacaaagatc ttgaggaaga
     2341 tgagggaagc cctcccctct tcacagtccc catctcttct cctttgtacc tgtcaacacc
     2401 agagttcagt gtttaaacag acaaaatata aagtattgag taggtggttc atatgccgaa
     2461 tccacttggt aggaagaaac caccagcttt atagtgtgcc tgatagattt taaacattcc
     2521 tgggcaccca ctcaagagtg ctctttttat caccttctgg aaatccgcaa agttgcaggg
     2581 gcctctggag tgtctcttct ctagagagaa ttggtgggac ccccctcagt gcagtggccc
     2641 caactagtgg agggagagag gacttaagtc agatggactc aacagaaatg ggtttccaga
     2701 agaataatga aaagttgtgg gtaggaaaat gaatcatttg gactcttcaa tgaaatggag
     2761 tgagcccagg agagctcagc caacagaggc actctgggaa cctgttagta aagccaggct
     2821 ggccaaatgc catttgattt tgaacctcgt aggtccccac tcaccctctg ccaggagcta
     2881 agtaaggcag gagagctgac ttgggactcc tggctcggcc ccaacaggga gcccccttcc
     2941 caccatccct cggcaagctc accacctcat ccttctgcca aggcagcttt cctttctttt
     3001 gtgtgttttc tgtgttctta gcctccaccc tcctcctgcc acccttgtgg actaggacca
     3061 ggtcctgacc ccagtcagaa aatgatgata tgtacagtgg cacaccttaa ccagtcacta
     3121 attttcactg ttgtgaaagt gatttgattt agaattaaac aaatggtttt acattactat
     3181 ga
//
