LOCUS       NM_016494                685 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens ring finger protein 181 (RNF181), mRNA.
ACCESSION   NM_016494
VERSION     NM_016494.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Li,Y., Xiang,Z.Y., Xiong,J., Hou,Z.W., Zhu,Z. and Bao,W.W.
  TITLE     RN181 regulates the biological behaviors of oral squamous cell
            carcinoma cells via mediating ERK/MAPK signaling pathway
  JOURNAL   Acta Histochem 123 (5), 151733 (2021)
   PUBMED   34052676
  REMARK    GeneRIF: RN181 regulates the biological behaviors of oral squamous
            cell carcinoma cells via mediating ERK/MAPK signaling pathway.
REFERENCE   2  (bases 1 to 685)
  AUTHORS   Zhu,J., Li,X., Su,P., Xue,M., Zang,Y. and Ding,Y.
  TITLE     The ubiquitin ligase RNF181 stabilizes ERalpha and modulates breast
            cancer progression
  JOURNAL   Oncogene 39 (44), 6776-6788 (2020)
   PUBMED   32973333
  REMARK    GeneRIF: The ubiquitin ligase RNF181 stabilizes ERalpha and
            modulates breast cancer progression.
REFERENCE   3  (bases 1 to 685)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 685)
  AUTHORS   Wang,S., Wang,X., Gao,Y., Peng,Y., Dong,N., Xie,Q., Zhang,X.,
            Wu,Y., Li,M. and Li,J.L.
  TITLE     RN181 is a tumour suppressor in gastric cancer by regulation of the
            ERK/MAPK-cyclin D1/CDK4 pathway
  JOURNAL   J Pathol 248 (2), 204-216 (2019)
   PUBMED   30714150
  REMARK    GeneRIF: RN181 functions as a tumour suppressor in gastric cancer
            by regulation of the ERK/MAPK-cyclin D1/CDK4 pathway, controlling
            cell proliferation and tumor volume.
REFERENCE   5  (bases 1 to 685)
  AUTHORS   Pedersen,S.M., Chan,W., Jattani,R.P., Mackie,d.S. and
            Pomerantz,J.L.
  TITLE     Negative Regulation of CARD11 Signaling and Lymphoma Cell Survival
            by the E3 Ubiquitin Ligase RNF181
  JOURNAL   Mol Cell Biol 36 (5), 794-808 (2015)
   PUBMED   26711259
  REMARK    GeneRIF: RNF181 limits the proliferation of human diffuse large B
            cell lymphoma cells that depend upon aberrant CARD11 signaling to
            NF-kappaB for growth and survival in culture.
            Erratum:[Mol Cell Biol. 2016 Mar 31;36(8):1322. doi:
            10.1128/MCB.00106-16.. PMID: 27034408]
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 685)
  AUTHORS   Wang,S., Huang,X., Li,Y., Lao,H., Zhang,Y., Dong,H., Xu,W., Li,J.L.
            and Li,M.
  TITLE     RN181 suppresses hepatocellular carcinoma growth by inhibition of
            the ERK/MAPK pathway
  JOURNAL   Hepatology 53 (6), 1932-1942 (2011)
   PUBMED   21391225
  REMARK    GeneRIF: RN181 suppresses the tumorigenesis of hepatocellular
            carcinoma through the inhibition of ERK/MAPK signaling in the
            liver.
REFERENCE   7  (bases 1 to 685)
  AUTHORS   Huang,X., Tan,J.Y., Qiu,Y.L., Liang,W.J., Zhou,M. and Li,M.
  TITLE     [Construction of recombinant eukaryotic expression vector
            pDsRed1-C3/LOC51255 and subcellular localization of LOC51255
            protein in HePG2 cell line]
  JOURNAL   Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 25 (5), 402-405 (2009)
   PUBMED   19426595
  REMARK    GeneRIF: Data show LOC51255 (RNF181) protein located in the
            cytoplasm of HePG2 cell line.
REFERENCE   8  (bases 1 to 685)
  AUTHORS   van Wijk,S.J., de Vries,S.J., Kemmeren,P., Huang,A., Boelens,R.,
            Bonvin,A.M. and Timmers,H.T.
  TITLE     A comprehensive framework of E2-RING E3 interactions of the human
            ubiquitin-proteasome system
  JOURNAL   Mol Syst Biol 5, 295 (2009)
   PUBMED   19690564
  REMARK    Erratum:[Mol Syst Biol. 2009;5:317]
REFERENCE   9  (bases 1 to 685)
  AUTHORS   Brophy,T.M., Raab,M., Daxecker,H., Culligan,K.G., Lehmann,I.,
            Chubb,A.J., Treumann,A. and Moran,N.
  TITLE     RN181, a novel ubiquitin E3 ligase that interacts with the KVGFFKR
            motif of platelet integrin alpha(IIb)beta3
  JOURNAL   Biochem Biophys Res Commun 369 (4), 1088-1093 (2008)
   PUBMED   18331836
  REMARK    GeneRIF: The presence of RN181 in human platelets was established;
            RN181 has ubiquitin E3 ligase activity.
REFERENCE   10 (bases 1 to 685)
  AUTHORS   Wang,A.G., Yoon,S.Y., Oh,J.H., Jeon,Y.J., Kim,M., Kim,J.M.,
            Byun,S.S., Yang,J.O., Kim,J.H., Kim,D.G., Yeom,Y.I., Yoo,H.S.,
            Kim,Y.S. and Kim,N.S.
  TITLE     Identification of intrahepatic cholangiocarcinoma related genes by
            comparison with normal liver tissues using expressed sequence tags
  JOURNAL   Biochem Biophys Res Commun 345 (3), 1022-1032 (2006)
   PUBMED   16712791
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC002803.2 and AC016753.9.
            
            On May 17, 2019 this sequence version replaced NM_016494.3.
            
            Summary: RNF181 binds the integrin alpha-IIb (ITGA2B; MIM
            607759)/beta-3 (ITGB3; MIM 173470) complex and has E3 ubiquitin
            ligase activity (Brophy et al., 2008 [PubMed 18331836]).[supplied
            by OMIM, Dec 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803616.284243.1, BG431440.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000306368.9/ ENSP00000306906.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-558               BC002803.2         7-564
            559-685             AC016753.9         164541-164667
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p11.2"
     gene            1..685
                     /gene="RNF181"
                     /gene_synonym="HSPC238"
                     /note="ring finger protein 181"
                     /db_xref="GeneID:51255"
                     /db_xref="HGNC:HGNC:28037"
                     /db_xref="MIM:612490"
     exon            1..105
                     /gene="RNF181"
                     /gene_synonym="HSPC238"
                     /inference="alignment:Splign:2.1.0"
     CDS             20..481
                     /gene="RNF181"
                     /gene_synonym="HSPC238"
                     /EC_number="2.3.2.27"
                     /note="RING-type E3 ubiquitin transferase RNF181"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase RNF181"
                     /protein_id="NP_057578.1"
                     /db_xref="CCDS:CCDS1981.1"
                     /db_xref="GeneID:51255"
                     /db_xref="HGNC:HGNC:28037"
                     /db_xref="MIM:612490"
                     /translation="MASYFDEHDCEPSDPEQETRTNMLLELARSLFNRMDFEDLGLVV
                     DWDHHLPPPAAKTVVENLPRTVIRGSQAELKCPVCLLEFEEEETAIEMPCHHLFHSSC
                     ILPWLSKTNSCPLCRYELPTDDDTYEEHRRDKARKQQQQHRLENLHGAMYT"
     exon            106..236
                     /gene="RNF181"
                     /gene_synonym="HSPC238"
                     /inference="alignment:Splign:2.1.0"
     exon            237..346
                     /gene="RNF181"
                     /gene_synonym="HSPC238"
                     /inference="alignment:Splign:2.1.0"
     exon            347..421
                     /gene="RNF181"
                     /gene_synonym="HSPC238"
                     /inference="alignment:Splign:2.1.0"
     exon            422..685
                     /gene="RNF181"
                     /gene_synonym="HSPC238"
                     /inference="alignment:Splign:2.1.0"
     regulatory      538..543
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RNF181"
                     /gene_synonym="HSPC238"
                     /note="hexamer: ATTAAA"
     polyA_site      558
                     /gene="RNF181"
                     /gene_synonym="HSPC238"
                     /note="major polyA site"
     polyA_site      605
                     /gene="RNF181"
                     /gene_synonym="HSPC238"
ORIGIN      
        1 gtcagaaggc tgggcagcca tggcgtccta tttcgatgaa cacgactgcg agccgtcgga
       61 ccctgagcag gagacgcgaa ccaacatgct gctggagctc gcaaggtcac ttttcaatag
      121 gatggacttt gaagacttgg ggttggtagt agattgggac caccacctgc ctccaccagc
      181 tgccaagact gtggttgaga acctccccag gacagtcatc agaggctctc aggctgagct
      241 caagtgcccc gtgtgtcttt tggaatttga ggaggaggag actgccattg agatgccttg
      301 ccatcacctt ttccattcca gctgcattct gccctggcta agcaagacaa attcctgtcc
      361 cttgtgccgc tatgagctgc ccactgatga cgacacttat gaggagcaca gacgagataa
      421 ggctcgaaaa cagcagcagc aacaccgact ggagaacctc catggagcca tgtacacgtg
      481 aggaggttgg ggctgagtgc tggccctctg cgtcttcctt attaaccttg aatcctcatt
      541 aaaggtttct ttacccaccc tgaggctgta ttgatcacag acctggccag gggctctgca
      601 tcctccatca ggtctctact tctgttgggg aaggtgatcc taaatcgcag aaggcaccag
      661 gctgcggttt tcctccctgc tggcc
//
