LOCUS       NM_152617               5347 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens ring finger protein 168 (RNF168), mRNA.
ACCESSION   NM_152617
VERSION     NM_152617.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Feng,L.L., Bie,S.Y., Deng,Z.H., Bai,S.M., Shi,J., Qin,C.L.,
            Liu,H.L., Li,J.X., Chen,W.Y., Zhou,J.Y., Jiao,C.M., Ma,Y.,
            Qiu,M.B., Ai,H.S., Zheng,J., Hung,M.C., Wang,Y.L., Wan,X.B. and
            Fan,X.J.
  TITLE     Ubiquitin-induced RNF168 condensation promotes DNA double-strand
            break repair
  JOURNAL   Proc Natl Acad Sci U S A 121 (28), e2322972121 (2024)
   PUBMED   38968116
  REMARK    GeneRIF: Ubiquitin-induced RNF168 condensation promotes DNA
            double-strand break repair.
REFERENCE   2  (bases 1 to 5347)
  AUTHORS   Qiu,L., Xu,W., Lu,X., Chen,F., Chen,Y., Tian,Y., Zhu,Q., Liu,X.,
            Wang,Y., Pei,X.H., Xu,X., Zhang,J. and Zhu,W.G.
  TITLE     The HDAC6-RNF168 axis regulates H2A/H2A.X ubiquitination to enable
            double-strand break repair
  JOURNAL   Nucleic Acids Res 51 (17), 9166-9182 (2023)
   PUBMED   37503842
  REMARK    GeneRIF: The HDAC6-RNF168 axis regulates H2A/H2A.X ubiquitination
            to enable double-strand break repair.
REFERENCE   3  (bases 1 to 5347)
  AUTHORS   Wei,M., Huang,X., Liao,L., Tian,Y. and Zheng,X.
  TITLE     SENP1 Decreases RNF168 Phase Separation to Promote DNA Damage
            Repair and Drug Resistance in Colon Cancer
  JOURNAL   Cancer Res 83 (17), 2908-2923 (2023)
   PUBMED   37350666
  REMARK    GeneRIF: SENP1 Decreases RNF168 Phase Separation to Promote DNA
            Damage Repair and Drug Resistance in Colon Cancer.
REFERENCE   4  (bases 1 to 5347)
  AUTHORS   Lee,S.O., Kelliher,J.L., Song,W., Tengler,K., Sarkar,A., Dray,E.
            and Leung,J.W.C.
  TITLE     UBA80 and UBA52 fine-tune RNF168-dependent histone ubiquitination
            and DNA repair
  JOURNAL   J Biol Chem 299 (8), 105043 (2023)
   PUBMED   37451480
  REMARK    GeneRIF: UBA80 and UBA52 fine-tune RNF168-dependent histone
            ubiquitination and DNA repair.
REFERENCE   5  (bases 1 to 5347)
  AUTHORS   Zou,L., Sun,L., Hua,R., Wu,Y., Sun,L. and Chen,T.
  TITLE     Degradation of Ubiquitin-Editing Enzyme A20 following Autophagy
            Activation Promotes RNF168 Nuclear Translocation and NF-kappaB
            Activation in Lupus Nephritis
  JOURNAL   J Innate Immun 15 (1), 428-441 (2023)
   PUBMED   36944318
  REMARK    GeneRIF: Degradation of Ubiquitin-Editing Enzyme A20 following
            Autophagy Activation Promotes RNF168 Nuclear Translocation and
            NF-kappaB Activation in Lupus Nephritis.
REFERENCE   6  (bases 1 to 5347)
  AUTHORS   Pinato,S., Scandiuzzi,C., Arnaudo,N., Citterio,E., Gaudino,G. and
            Penengo,L.
  TITLE     RNF168, a new RING finger, MIU-containing protein that modifies
            chromatin by ubiquitination of histones H2A and H2AX
  JOURNAL   BMC Mol Biol 10, 55 (2009)
   PUBMED   19500350
  REMARK    GeneRIF: RNF168 is a ubiquitin ligase that functions as chromatin
            modifier, through histone ubiquitination; upon DNA lesions, RNF168
            is recruited to DNA damage response foci where it contributes to
            increase the amount of ubiquitinated proteins.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 5347)
  AUTHORS   Doil,C., Mailand,N., Bekker-Jensen,S., Menard,P., Larsen,D.H.,
            Pepperkok,R., Ellenberg,J., Panier,S., Durocher,D., Bartek,J.,
            Lukas,J. and Lukas,C.
  TITLE     RNF168 binds and amplifies ubiquitin conjugates on damaged
            chromosomes to allow accumulation of repair proteins
  JOURNAL   Cell 136 (3), 435-446 (2009)
   PUBMED   19203579
  REMARK    GeneRIF: RNF168 defines a new pathway involving sequential
            ubiquitylations on damaged chromosomes and uncovers a functional
            cooperation between E3 ligases in genome maintenance.
REFERENCE   8  (bases 1 to 5347)
  AUTHORS   Stewart,G.S., Panier,S., Townsend,K., Al-Hakim,A.K., Kolas,N.K.,
            Miller,E.S., Nakada,S., Ylanko,J., Olivarius,S., Mendez,M.,
            Oldreive,C., Wildenhain,J., Tagliaferro,A., Pelletier,L.,
            Taubenheim,N., Durandy,A., Byrd,P.J., Stankovic,T., Taylor,A.M. and
            Durocher,D.
  TITLE     The RIDDLE syndrome protein mediates a ubiquitin-dependent
            signaling cascade at sites of DNA damage
  JOURNAL   Cell 136 (3), 420-434 (2009)
   PUBMED   19203578
REFERENCE   9  (bases 1 to 5347)
  AUTHORS   Penengo,L., Mapelli,M., Murachelli,A.G., Confalonieri,S., Magri,L.,
            Musacchio,A., Di Fiore,P.P., Polo,S. and Schneider,T.R.
  TITLE     Crystal structure of the ubiquitin binding domains of rabex-5
            reveals two modes of interaction with ubiquitin
  JOURNAL   Cell 124 (6), 1183-1195 (2006)
   PUBMED   16499958
REFERENCE   10 (bases 1 to 5347)
  AUTHORS   Colland,F., Jacq,X., Trouplin,V., Mougin,C., Groizeleau,C.,
            Hamburger,A., Meil,A., Wojcik,J., Legrain,P. and Gauthier,J.M.
  TITLE     Functional proteomics mapping of a human signaling pathway
  JOURNAL   Genome Res 14 (7), 1324-1332 (2004)
   PUBMED   15231748
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK054732.1, BC033791.1,
            AC117490.3 and BC047483.1.
            
            On May 17, 2019 this sequence version replaced NM_152617.3.
            
            Summary: This gene encodes an E3 ubiquitin ligase protein that
            contains a RING finger, a motif present in a variety of
            functionally distinct proteins and known to be involved in
            protein-DNA and protein-protein interactions. The protein is
            involved in DNA double-strand break (DSB) repair. Mutations in this
            gene result in Riddle syndrome. [provided by RefSeq, Sep 2011].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK054732.1, SRR14038197.1166991.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000318037.3/ ENSP00000320898.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-620               AK054732.1         1-620
            621-2373            BC033791.1         564-2316
            2374-4327           AC117490.3         26583-28536         c
            4328-5347           BC047483.1         312-1331
FEATURES             Location/Qualifiers
     source          1..5347
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q29"
     gene            1..5347
                     /gene="RNF168"
                     /gene_synonym="hRNF168; RIDL"
                     /note="ring finger protein 168"
                     /db_xref="GeneID:165918"
                     /db_xref="HGNC:HGNC:26661"
                     /db_xref="MIM:612688"
     exon            1..896
                     /gene="RNF168"
                     /gene_synonym="hRNF168; RIDL"
                     /inference="alignment:Splign:2.1.0"
     CDS             596..2311
                     /gene="RNF168"
                     /gene_synonym="hRNF168; RIDL"
                     /EC_number="2.3.2.27"
                     /note="ring finger protein 168, E3 ubiquitin protein
                     ligase; RING-type E3 ubiquitin transferase RNF168"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase RNF168"
                     /protein_id="NP_689830.2"
                     /db_xref="CCDS:CCDS3317.1"
                     /db_xref="GeneID:165918"
                     /db_xref="HGNC:HGNC:26661"
                     /db_xref="MIM:612688"
                     /translation="MALPKDAIPSLSECQCGICMEILVEPVTLPCNHTLCKPCFQSTV
                     EKASLCCPFCRRRVSSWTRYHTRRNSLVNVELWTIIQKHYPRECKLRASGQESEEVAD
                     DYQPVRLLSKPGELRREYEEEISKVAAERRASEEEENKASEEYIQRLLAEEEEEEKRQ
                     AEKRRRAMEEQLKSDEELARKLSIDINNFCEGSISASPLNSRKSDPVTPKSEKKSKNK
                     QRNTGDIQKYLTPKSQFGSASHSEAVQEVRKDSVSKDIDSSDRKSPTGQDTEIEDMPT
                     LSPQISLGVGEQGADSSIESPMPWLCACGAEWYHEGNVKTRPSNHGKELCVLSHERPK
                     TRVPYSKETAVMPCGRTESGCAPTSGVTQTNGNNTGETENEESCLLISKEISKRKNQE
                     SSFEAVKDPCFSAKRRKVSPESSPDQEETEINFTQKLIDLEHLLFERHKQEEQDRLLA
                     LQLQKEVDKEQMVPNRQKGSPDEYHLRATSSPPDKVLNGQRKNPKDGNFKRQTHTKHP
                     TPERGSRDKNRQVSLKMQLKQSVNRRKMPNSTRDHCKVSKSAHSLQPSISQKSVFQMF
                     QRCTK"
     exon            897..973
                     /gene="RNF168"
                     /gene_synonym="hRNF168; RIDL"
                     /inference="alignment:Splign:2.1.0"
     exon            974..1153
                     /gene="RNF168"
                     /gene_synonym="hRNF168; RIDL"
                     /inference="alignment:Splign:2.1.0"
     exon            1154..1275
                     /gene="RNF168"
                     /gene_synonym="hRNF168; RIDL"
                     /inference="alignment:Splign:2.1.0"
     exon            1276..1357
                     /gene="RNF168"
                     /gene_synonym="hRNF168; RIDL"
                     /inference="alignment:Splign:2.1.0"
     exon            1358..5347
                     /gene="RNF168"
                     /gene_synonym="hRNF168; RIDL"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5205..5210
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RNF168"
                     /gene_synonym="hRNF168; RIDL"
                     /note="hexamer: AATAAA"
     polyA_site      5227
                     /gene="RNF168"
                     /gene_synonym="hRNF168; RIDL"
                     /note="major polyA site"
     regulatory      5326..5331
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RNF168"
                     /gene_synonym="hRNF168; RIDL"
                     /note="hexamer: AATAAA"
     polyA_site      5347
                     /gene="RNF168"
                     /gene_synonym="hRNF168; RIDL"
ORIGIN      
        1 gcttgtccgg tggctgctgc gctgccgcaa cgaatagggt ttctggctgc gtaggaggga
       61 cgggggcgcg gagctctggg aaactgcgcc aggcgcccga aaggtgaaca cgggagtcgc
      121 gcgtctcccc cgcagcagcg gtaaagcgga agttatgctg cagccggagc ccgggcttcc
      181 tcccggagcc gcgtcccggg gcccggctgc cccgagctga gcggagcatc ctttccgggt
      241 gaggggagga gaggacttgg cgcgttcccc tcgctgcccc gggagccgca gccgcggtgt
      301 tcatgccgcg gagcagccag gctcctccga cgaaaacctg catttatttg ctggcgggac
      361 gtttgccttg aaaatggaca aagacgccgc cctccggggt attcctgttt gcctgaccct
      421 gagagcgcct ttttgcttca agacgtgttg gatgctcctg ttctccgaat tctgatacgc
      481 ttctgggcat aatactgaaa cacaaaactg cttttgctct ctctgtggtt ggccgaaaat
      541 aggattcttt ttcgtgcagg tgtcgttgtt tagtcggctt tactaacata ttgaaatggc
      601 tctacccaaa gacgccatcc cctcgctgtc cgagtgccag tgcgggatct gcatggaaat
      661 cctcgtggag cccgtcaccc tcccgtgtaa ccacacgctg tgtaaaccgt gcttccagtc
      721 gaccgtcgaa aaggcgagtt tatgctgtcc cttctgtcgc cgccgggtat cgtcgtggac
      781 tcggtaccat acccgaagaa attctctcgt caacgtggaa ctgtggacga taattcaaaa
      841 acactatccc agggagtgca agcttagagc gtctggccaa gaatcagagg aagtggctga
      901 tgactatcag ccagttcgtc tgctcagtaa acctggggaa ctgagaagag aatatgaaga
      961 ggaaataagc aaggtggcgg cagagcgacg ggccagcgag gaagaagaaa acaaagccag
     1021 tgaagaatac atacagaggt tgttggcaga ggaggaagaa gaggaaaaaa gacaggcaga
     1081 aaaaaggcga agagcgatgg aagaacaact gaaaagtgat gaggaactgg caagaaagct
     1141 aagcattgat attaacaatt tctgtgaggg aagtatctcg gcttctccct tgaattccag
     1201 aaaatctgat ccagttacac ccaagtctga aaagaaaagt aagaacaaac aaagaaacac
     1261 tggagatatt cagaagtatt tgacaccgaa atctcagttt gggtcagcct cacactctga
     1321 agctgtacaa gaagtcagga aagactccgt atctaaggac attgacagta gtgataggaa
     1381 aagcccaaca gggcaagaca cagaaataga agatatgccg acactttctc cacagatatc
     1441 ccttggagtt ggagaacaag gtgcagattc ttcaatagag tcccctatgc catggttatg
     1501 tgcctgtggt gccgaatggt accatgaagg aaacgtcaaa acaagaccaa gcaatcatgg
     1561 gaaagagtta tgtgtcttaa gtcacgagcg acctaaaacc agagttccct actcgaaaga
     1621 aactgcagtt atgccttgtg gcagaacaga aagtgggtgc gcccccacat caggggtgac
     1681 acagacaaat ggaaacaaca caggtgagac agaaaatgaa gagtcgtgcc tactgatcag
     1741 taaggagatt tccaaaagaa aaaaccaaga atcttccttt gaagcagtca aggatccatg
     1801 cttttctgca aaaagaagaa aagtgtcccc cgaatcttcc ccagatcaag aggaaacaga
     1861 aataaacttt acccaaaaac tgatagattt ggagcatcta ctgtttgaga gacataaaca
     1921 agaagaacag gacaggttat tggcattaca acttcagaag gaggtggata aagagcaaat
     1981 ggtgccaaac cggcaaaaag gatccccaga tgagtatcac ttacgcgcta catcctcccc
     2041 tccagacaaa gtgctaaatg gacagaggaa gaatcccaaa gatgggaact tcaaaaggca
     2101 aactcacaca aagcatccaa caccagagag aggctcaagg gacaaaaata ggcaagtgtc
     2161 tttaaagatg cagttgaagc agtcagttaa tagaagaaag atgccaaatt ctactagaga
     2221 tcactgtaag gtatccaaaa gtgctcactc cctacagcct agcatttcac agaaaagtgt
     2281 ttttcagatg tttcagagat gcacaaagta aggcctggta aagggagtgc tttgtgatct
     2341 agtaaagctg gaatgtgaag ctctttccta tcatggaatc ttcatccatg tgtcattcat
     2401 gctgctctct aggcacactc attgtcctta atgaaggtct gtgtgattgt aaggactgca
     2461 tagacacatg agcttctgca cagctcagcg gtaagcaggg gtcccctgtc gtttcccttc
     2521 ttaataacag atgtgtatta agctttacaa ttcttagaga tatcttaagc tttggccaac
     2581 tccaggaaat cttcaagtgc cattgtcttt ccaaaaactg catgttttat ggcctttgct
     2641 cacctttttg tcgtgactgg taatcaaagc aagattatac tattctgagt gattaaggaa
     2701 cccatgcagg gtttaaaatc cattttcttg ctattgtcca atgtttacag agtactcaaa
     2761 aggttttcct aaagtaatct aatttccatc tccaatttaa ttcaattttc agcattgtag
     2821 catttccttt ggtgtttgtt tttttttttt agacggagtt tcaaaatttg gattcatatt
     2881 catatgtggg aaatcccaga tttttttttt tttttttttt tttttttttt tttggagaca
     2941 gagtcttgct ctgtcacgca ggctggtgtg caatggcgcg atctcagctc actgcaacct
     3001 ccgcctcccg agttcaagcg attcttctgc ctcagcctcc cgagtagctg ggatcatagg
     3061 catgtgccac tacacccagc tacccagcta attttttttt ttttttgtac ttttagtaga
     3121 gatgggattt caccatgttg gccaggctgg tcttgaactc ctgtcctcag gtgatccacc
     3181 tgcttcggcc tcccagagtg ctgggattat aggcgtgagc caccacgccc ggcctgtttc
     3241 ctttggttta aagtggattc gaatcagtgt taaatcagaa aatcagggtt ttctaggaaa
     3301 ttgggaatta gttgccagaa aggaaactga actatgcaga aggagttaag agctgggatg
     3361 aggctggatg gggtgactga cagtccagat cattgaaaca gattgggtcg tcggaaactg
     3421 gtccagatga ggctggatgg ggtgactgac agtctggatc attgaaactg attaggtcgt
     3481 cagaaacggg tccagacgag gatgaggctg aggctggatg gggtgactga cagtctggat
     3541 cattgaaact gattgggtgg ttggtcagaa acgggtccag atgaggatga ggctgaggct
     3601 ggatggggtg actgacagtc tggatcattg aaactgattg ggtggttggt cagaaacggg
     3661 tccagatgag gatgaggctg gatggggtga ctgacagtct ggatcattga aactgattgg
     3721 gtcgtcagaa actggtccag atgaggctgg gtggggtgac tgacagtttg gatcattgaa
     3781 actgattggg tggtcagaaa ctggtccaga tgaggatgtg gctggatggg gtgactgaca
     3841 gtctggatca ttgaaactga ttgggtggtc agaaactggt ccagatgagg atgtggctgg
     3901 atggggtgac tgacagtctg gatcattgaa actgattggg tggtgagaaa tgggtccaga
     3961 tgaggatgag gctggatggg gtgactgaca gtctggatca ttgaaactga ttgggtggtg
     4021 agaaatgggt ccagatgagg atgaggctgg atggggtgac tgacagtctg gatcattgaa
     4081 actggtccac tatgacgcag caggggcagt ttggcagagc atcctcttcc cttgtccttg
     4141 tgaaagaaca caggaaacat ttgggtttta cagcttttca caccctacta tcaggcatca
     4201 gaatcaaaat tagttactaa attgggtgaa tttttggaaa cctgcatttc tatgcatctg
     4261 ctattccaga aaaaaatggg aatgtattac aggagagtta cctcacagtg tcttatgttg
     4321 tggaacctga aacataacca cgtgttttct tatatgatca tttgtgttga tactgctttt
     4381 atttgagttt atgaacagaa atagaaagta tggtgcttgg gttttgccct ttcttactcc
     4441 tgaaagttaa atcagaagac actgatttca ttttgtgaaa tttagctcag agactattga
     4501 tcttttgttt cattaatatg aacaactatt agtaaaaaat agctttaaca gcatttctgc
     4561 tgatatctag taatctattc ttttaatgtg aaaataagat aaaatgtcct ggagctaatt
     4621 ctagcttaaa tttgccagta tttctgtatg tcattaagtt tttttcctct aaggttggta
     4681 atagaatttt gttaatcttt gcatacctga tggcatctat gtcaatgctg attgggtaat
     4741 tataaattct gtgctaattt aaaacttaat ttgcctctta aggtgattgt cctctgagta
     4801 atgattgtag ttaaatgaag tatagcttgc aactatacta tcacatgggt cgttaagtaa
     4861 aaataaataa accaaatttg tctgagacag gctaagatca atcttctcat caaaccaatt
     4921 tttctctaag agcaatttca ctttcagttt tagggtggac attcttgaat gcctcaaatt
     4981 aaacgttatc tatttaatct tcctggaata gtctgtgacc aaaaaggagg gtgtgatata
     5041 tttaggtgta aatatatcac atatatggtg tgatatattt gggatttata tattcagctc
     5101 attctctgtg aagaagtctt cctgactaaa attggtttca agataaacta atttctgtta
     5161 gtatttctac tctgcctacc atgtatgcct ttttgttaga aactaataaa tgtatcagtc
     5221 actagcaggt actggtcctt tttcctctca tttttgtgga aaggatatgc aaacactgaa
     5281 tttctcaggg agtactaagg ctgtgcagag gctgagaatc tgaaaaataa aatactaaaa
     5341 ataaaaa
//
