LOCUS       NM_194463               2803 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens ring finger protein 128 (RNF128), transcript variant
            1, mRNA.
ACCESSION   NM_194463
VERSION     NM_194463.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Ray,P., Jaiswal,S., Ferrer-Torres,D., Wang,Z., Nancarrow,D.,
            Curtin,M., Martinho,M.S., Lacy,S.M., Kasturirangan,S., Thomas,D.,
            Spence,J.R., Truttmann,M.C., Lagisetty,K.H., Lawrence,T.S.,
            Wang,T.D., Beer,D.G. and Ray,D.
  TITLE     GRAIL1 Stabilizes Misfolded Mutant p53 through a Ubiquitin
            Ligase-Independent, Chaperone Regulatory Function
  JOURNAL   Mol Cancer Res 22 (11), 996-1010 (2024)
   PUBMED   39018356
  REMARK    GeneRIF: GRAIL1 Stabilizes Misfolded Mutant p53 through a Ubiquitin
            Ligase-Independent, Chaperone Regulatory Function.
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Huang,S., Zhao,J., Yu,H. and Chen,G.
  TITLE     Mechanism of tumor-derived extracellular vesicles in prostatic
            cancer progression through the circFMN2/KLF2/RNF128 axis
  JOURNAL   Apoptosis 28 (9-10), 1372-1389 (2023)
   PUBMED   37452271
  REMARK    GeneRIF: Mechanism of tumor-derived extracellular vesicles in
            prostatic cancer progression through the circFMN2/KLF2/RNF128 axis.
REFERENCE   3  (bases 1 to 2803)
  AUTHORS   Fujimoto,S., Kyuichi,K., Kaede,Y., Chihiro,Y., Emi,I., Ryo,I.,
            Reiji,H., Toshiki,Y. and Yokomise,H.
  TITLE     RNF128 expression in lung adenocarcinoma is a favorable prognostic
            factor associated with decreased tumor-associated macrophages
  JOURNAL   Thorac Cancer 14 (17), 1581-1588 (2023)
   PUBMED   37186218
  REMARK    GeneRIF: RNF128 expression in lung adenocarcinoma is a favorable
            prognostic factor associated with decreased tumor-associated
            macrophages.
REFERENCE   4  (bases 1 to 2803)
  AUTHORS   Zhuang,Y., Liu,P.F., Zhan,Y., Kong,D.L., Tian,F. and Zhao,P.
  TITLE     RING finger protein 128 (RNF128) regulates malignant biological
            behaviors of colorectal cancer cells via PI3K/AKT signaling pathway
  JOURNAL   Cell Biol Int 46 (10), 1604-1611 (2022)
   PUBMED   35723244
  REMARK    GeneRIF: RING finger protein 128 (RNF128) regulates malignant
            biological behaviors of colorectal cancer cells via PI3K/AKT
            signaling pathway.
REFERENCE   5  (bases 1 to 2803)
  AUTHORS   Ray,P., Nancarrow,D.J., Ferrer-Torres,D., Wang,Z., San Martinho,M.,
            Hinton,T., Wu,J.H., Wu,A., Turgeon,D.K., Hammer,M.A., Dame,M.K.,
            Lawrence,T.S., O'Brien,P.J., Spence,J.R., Beer,D.G. and Ray,D.
  TITLE     UBCH5 Family Members Differentially Impact Stabilization of Mutant
            p53 via RNF128 Iso1 During Barrett's Progression to Esophageal
            Adenocarcinoma
  JOURNAL   Cell Mol Gastroenterol Hepatol 13 (1), 129-149 (2022)
   PUBMED   34416429
  REMARK    GeneRIF: UBCH5 Family Members Differentially Impact Stabilization
            of Mutant p53 via RNF128 Iso1 During Barrett's Progression to
            Esophageal Adenocarcinoma.
REFERENCE   6  (bases 1 to 2803)
  AUTHORS   Kostianovsky,A.M., Maier,L.M., Baecher-Allan,C., Anderson,A.C. and
            Anderson,D.E.
  TITLE     Up-regulation of gene related to anergy in lymphocytes is
            associated with Notch-mediated human T cell suppression
  JOURNAL   J Immunol 178 (10), 6158-6163 (2007)
   PUBMED   17475842
  REMARK    GeneRIF: Inhibition of T cell activation is not due to apoptosis or
            disruption of proximal T- cell receptor signaling, but is
            associated with up-regulation of GRAIL in CD4+ T cells, with modest
            effects on other E3 ubiquitin ligases.
REFERENCE   7  (bases 1 to 2803)
  AUTHORS   MacKenzie,D.A., Schartner,J., Lin,J., Timmel,A., Jennens-Clough,M.,
            Fathman,C.G. and Seroogy,C.M.
  TITLE     GRAIL is up-regulated in CD4+ CD25+ T regulatory cells and is
            sufficient for conversion of T cells to a regulatory phenotype
  JOURNAL   J Biol Chem 282 (13), 9696-9702 (2007)
   PUBMED   17259178
  REMARK    GeneRIF: Forced expression of GRAIL in a T cell line from a T-cell
            receptor transgenic mouse is sufficient for conversion of these
            cells to a regulatory phenotype.
REFERENCE   8  (bases 1 to 2803)
  AUTHORS   Ross,M.T., Grafham,D.V., Coffey,A.J., Scherer,S., McLay,K.,
            Muzny,D., Platzer,M., Howell,G.R., Burrows,C., Bird,C.P.,
            Frankish,A., Lovell,F.L., Howe,K.L., Ashurst,J.L., Fulton,R.S.,
            Sudbrak,R., Wen,G., Jones,M.C., Hurles,M.E., Andrews,T.D.,
            Scott,C.E., Searle,S., Ramser,J., Whittaker,A., Deadman,R.,
            Carter,N.P., Hunt,S.E., Chen,R., Cree,A., Gunaratne,P., Havlak,P.,
            Hodgson,A., Metzker,M.L., Richards,S., Scott,G., Steffen,D.,
            Sodergren,E., Wheeler,D.A., Worley,K.C., Ainscough,R.,
            Ambrose,K.D., Ansari-Lari,M.A., Aradhya,S., Ashwell,R.I.,
            Babbage,A.K., Bagguley,C.L., Ballabio,A., Banerjee,R., Barker,G.E.,
            Barlow,K.F., Barrett,I.P., Bates,K.N., Beare,D.M., Beasley,H.,
            Beasley,O., Beck,A., Bethel,G., Blechschmidt,K., Brady,N.,
            Bray-Allen,S., Bridgeman,A.M., Brown,A.J., Brown,M.J., Bonnin,D.,
            Bruford,E.A., Buhay,C., Burch,P., Burford,D., Burgess,J.,
            Burrill,W., Burton,J., Bye,J.M., Carder,C., Carrel,L., Chako,J.,
            Chapman,J.C., Chavez,D., Chen,E., Chen,G., Chen,Y., Chen,Z.,
            Chinault,C., Ciccodicola,A., Clark,S.Y., Clarke,G., Clee,C.M.,
            Clegg,S., Clerc-Blankenburg,K., Clifford,K., Cobley,V., Cole,C.G.,
            Conquer,J.S., Corby,N., Connor,R.E., David,R., Davies,J., Davis,C.,
            Davis,J., Delgado,O., Deshazo,D., Dhami,P., Ding,Y., Dinh,H.,
            Dodsworth,S., Draper,H., Dugan-Rocha,S., Dunham,A., Dunn,M.,
            Durbin,K.J., Dutta,I., Eades,T., Ellwood,M., Emery-Cohen,A.,
            Errington,H., Evans,K.L., Faulkner,L., Francis,F., Frankland,J.,
            Fraser,A.E., Galgoczy,P., Gilbert,J., Gill,R., Glockner,G.,
            Gregory,S.G., Gribble,S., Griffiths,C., Grocock,R., Gu,Y.,
            Gwilliam,R., Hamilton,C., Hart,E.A., Hawes,A., Heath,P.D.,
            Heitmann,K., Hennig,S., Hernandez,J., Hinzmann,B., Ho,S., Hoffs,M.,
            Howden,P.J., Huckle,E.J., Hume,J., Hunt,P.J., Hunt,A.R.,
            Isherwood,J., Jacob,L., Johnson,D., Jones,S., de Jong,P.J.,
            Joseph,S.S., Keenan,S., Kelly,S., Kershaw,J.K., Khan,Z.,
            Kioschis,P., Klages,S., Knights,A.J., Kosiura,A., Kovar-Smith,C.,
            Laird,G.K., Langford,C., Lawlor,S., Leversha,M., Lewis,L., Liu,W.,
            Lloyd,C., Lloyd,D.M., Loulseged,H., Loveland,J.E., Lovell,J.D.,
            Lozado,R., Lu,J., Lyne,R., Ma,J., Maheshwari,M., Matthews,L.H.,
            McDowall,J., McLaren,S., McMurray,A., Meidl,P., Meitinger,T.,
            Milne,S., Miner,G., Mistry,S.L., Morgan,M., Morris,S., Muller,I.,
            Mullikin,J.C., Nguyen,N., Nordsiek,G., Nyakatura,G., O'Dell,C.N.,
            Okwuonu,G., Palmer,S., Pandian,R., Parker,D., Parrish,J.,
            Pasternak,S., Patel,D., Pearce,A.V., Pearson,D.M., Pelan,S.E.,
            Perez,L., Porter,K.M., Ramsey,Y., Reichwald,K., Rhodes,S.,
            Ridler,K.A., Schlessinger,D., Schueler,M.G., Sehra,H.K.,
            Shaw-Smith,C., Shen,H., Sheridan,E.M., Shownkeen,R., Skuce,C.D.,
            Smith,M.L., Sotheran,E.C., Steingruber,H.E., Steward,C.A.,
            Storey,R., Swann,R.M., Swarbreck,D., Tabor,P.E., Taudien,S.,
            Taylor,T., Teague,B., Thomas,K., Thorpe,A., Timms,K., Tracey,A.,
            Trevanion,S., Tromans,A.C., d'Urso,M., Verduzco,D., Villasana,D.,
            Waldron,L., Wall,M., Wang,Q., Warren,J., Warry,G.L., Wei,X.,
            West,A., Whitehead,S.L., Whiteley,M.N., Wilkinson,J.E.,
            Willey,D.L., Williams,G., Williams,L., Williamson,A.,
            Williamson,H., Wilming,L., Woodmansey,R.L., Wray,P.W., Yen,J.,
            Zhang,J., Zhou,J., Zoghbi,H., Zorilla,S., Buck,D., Reinhardt,R.,
            Poustka,A., Rosenthal,A., Lehrach,H., Meindl,A., Minx,P.J.,
            Hillier,L.W., Willard,H.F., Wilson,R.K., Waterston,R.H., Rice,C.M.,
            Vaudin,M., Coulson,A., Nelson,D.L., Weinstock,G., Sulston,J.E.,
            Durbin,R., Hubbard,T., Gibbs,R.A., Beck,S., Rogers,J. and
            Bentley,D.R.
  TITLE     The DNA sequence of the human X chromosome
  JOURNAL   Nature 434 (7031), 325-337 (2005)
   PUBMED   15772651
REFERENCE   9  (bases 1 to 2803)
  AUTHORS   Soares,L., Seroogy,C., Skrenta,H., Anandasabapathy,N., Lovelace,P.,
            Chung,C.D., Engleman,E. and Fathman,C.G.
  TITLE     Two isoforms of otubain 1 regulate T cell anergy via GRAIL
  JOURNAL   Nat Immunol 5 (1), 45-54 (2004)
   PUBMED   14661020
REFERENCE   10 (bases 1 to 2803)
  AUTHORS   Anandasabapathy,N., Ford,G.S., Bloom,D., Holness,C., Paragas,V.,
            Seroogy,C., Skrenta,H., Hollenhorst,M., Fathman,C.G. and Soares,L.
  TITLE     GRAIL: an E3 ubiquitin ligase that inhibits cytokine gene
            transcription is expressed in anergic CD4+ T cells
  JOURNAL   Immunity 18 (4), 535-547 (2003)
   PUBMED   12705856
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK074264.1, BI600046.1 and
            BC012931.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_194463.1.
            
            Summary: The protein encoded by this gene is a type I transmembrane
            protein that localizes to the endocytic pathway. This protein
            contains a RING zinc-finger motif and has been shown to possess E3
            ubiquitin ligase activity. Expression of this gene in retrovirally
            transduced T cell hybridoma significantly inhibits
            activation-induced IL2 and IL4 cytokine production. Induced
            expression of this gene was observed in anergic CD4(+) T cells,
            which suggested a role in the induction of anergic phenotype.
            Alternatively spliced transcript variants encoding distinct
            isoforms have been reported. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.179438.1, AK074264.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968832
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000255499.3/ ENSP00000255499.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-53                AK074264.1         38-90
            54-67               BI600046.1         51-64
            68-1328             AK074264.1         105-1365
            1329-2803           BC012931.1         321-1795
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq22.3"
     gene            1..2803
                     /gene="RNF128"
                     /gene_synonym="GRAIL"
                     /note="ring finger protein 128"
                     /db_xref="GeneID:79589"
                     /db_xref="HGNC:HGNC:21153"
                     /db_xref="MIM:300439"
     exon            1..697
                     /gene="RNF128"
                     /gene_synonym="GRAIL"
                     /inference="alignment:Splign:2.1.0"
     CDS             214..1500
                     /gene="RNF128"
                     /gene_synonym="GRAIL"
                     /EC_number="2.3.2.27"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; E3 ubiquitin-protein ligase RNF128; gene
                     related to anergy in lymphocytes protein; RING-type E3
                     ubiquitin transferase RNF128; ring finger protein 128, E3
                     ubiquitin protein ligase"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase RNF128 isoform 1
                     precursor"
                     /protein_id="NP_919445.1"
                     /db_xref="CCDS:CCDS14521.1"
                     /db_xref="GeneID:79589"
                     /db_xref="HGNC:HGNC:21153"
                     /db_xref="MIM:300439"
                     /translation="MGPPPGAGVSCRGGCGFSRLLAWCFLLALSPQAPGSRGAEAVWT
                     AYLNVSWRVPHTGVNRTVWELSEEGVYGQDSPLEPVAGVLVPPDGPGALNACNPHTNF
                     TVPTVWGSTVQVSWLALIQRGGGCTFADKIHLAYERGASGAVIFNFPGTRNEVIPMSH
                     PGAVDIVAIMIGNLKGTKILQSIQRGIQVTMVIEVGKKHGPWVNHYSIFFVSVSFFII
                     TAATVGYFIFYSARRLRNARAQSRKQRQLKADAKKAIGRLQLRTLKQGDKEIGPDGDS
                     CAVCIELYKPNDLVRILTCNHIFHKTCVDPWLLEHRTCPMCKCDILKALGIEVDVEDG
                     SVSLQVPVSNEISNSASSHEEDNRSETASSGYASVQGTDEPPLEEHVQSTNESLQLVN
                     HEANSVAVDVIPHVDNPTFEEDETPNQETAVREIKS"
     exon            698..945
                     /gene="RNF128"
                     /gene_synonym="GRAIL"
                     /inference="alignment:Splign:2.1.0"
     exon            946..1017
                     /gene="RNF128"
                     /gene_synonym="GRAIL"
                     /inference="alignment:Splign:2.1.0"
     exon            1018..1100
                     /gene="RNF128"
                     /gene_synonym="GRAIL"
                     /inference="alignment:Splign:2.1.0"
     exon            1101..1197
                     /gene="RNF128"
                     /gene_synonym="GRAIL"
                     /inference="alignment:Splign:2.1.0"
     exon            1198..1366
                     /gene="RNF128"
                     /gene_synonym="GRAIL"
                     /inference="alignment:Splign:2.1.0"
     exon            1367..2803
                     /gene="RNF128"
                     /gene_synonym="GRAIL"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2750..2755
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RNF128"
                     /gene_synonym="GRAIL"
                     /note="hexamer: TATAAA"
     regulatory      2761..2766
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RNF128"
                     /gene_synonym="GRAIL"
                     /note="hexamer: AATAAA"
     regulatory      2774..2779
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RNF128"
                     /gene_synonym="GRAIL"
                     /note="hexamer: AATAAA"
     polyA_site      2775
                     /gene="RNF128"
                     /gene_synonym="GRAIL"
     polyA_site      2780
                     /gene="RNF128"
                     /gene_synonym="GRAIL"
                     /note="major polyA site"
     polyA_site      2803
                     /gene="RNF128"
                     /gene_synonym="GRAIL"
ORIGIN      
        1 gcggtagcgg agaagactgg agctccgagg agctgcatct gcggcaacct gtgtgctgac
       61 gctacgtgcc tcctggctcc gacgtagctc gcagctcccc agtctcactc cattccttcc
      121 ccacctggcg cgcacctgct caagaccagg gtcctgccaa gcgctaggag ggcgcgtgcc
      181 aggggcgcta gggaactgcg gagcgcgcgc gccatggggc cgccgcctgg ggccggggtc
      241 tcctgccgcg gtggctgcgg cttttccaga ttgctggcat ggtgcttcct gctggccctg
      301 agtccgcagg cacccggttc ccggggggct gaagcagtgt ggaccgcgta cctcaacgtg
      361 tcctggcggg ttccgcacac gggagtgaac cgtacggtgt gggagctgag cgaggagggc
      421 gtgtacggcc aggactcgcc gctggagcct gtggctgggg tcctggtacc gcccgacggg
      481 cccggggcgc ttaacgcctg taacccgcac acgaatttca cggtgcccac ggtttgggga
      541 agcaccgtgc aagtctcttg gttggccctc atccaacgcg gcgggggctg caccttcgca
      601 gacaagatcc atctggctta tgagagaggg gcgtctggag ccgtcatctt taacttcccc
      661 gggacccgca atgaggtcat ccccatgtct cacccgggtg cagtagacat tgttgcaatc
      721 atgatcggca atctgaaagg cacaaaaatt ctgcaatcta ttcaaagagg catacaagtg
      781 acaatggtca tagaagtagg gaaaaaacat ggcccttggg tgaatcacta ttcaattttt
      841 ttcgtttctg tgtccttttt tattattacg gcggcaactg tgggctattt tatcttttat
      901 tctgctcgaa ggctacggaa tgcaagagct caaagcagga agcagaggca attaaaggca
      961 gatgctaaaa aagctattgg aaggcttcaa ctacgcacac tgaaacaagg agacaaggaa
     1021 attggccctg atggagatag ttgtgctgtg tgcattgaat tgtataaacc aaatgatttg
     1081 gtacgcatct taacgtgcaa ccatattttc cataagacat gtgttgaccc atggctgtta
     1141 gaacacagga cttgccccat gtgcaaatgt gacatactca aagctttggg aattgaggtg
     1201 gatgttgaag atggatcagt gtctttacaa gtccctgtat ccaatgaaat atctaatagt
     1261 gcctcctccc atgaagagga taatcgcagc gagaccgcat catctggata tgcttcagta
     1321 cagggaacag atgaaccgcc tctggaggaa cacgtgcagt caacaaatga aagtctacag
     1381 ctggtaaacc atgaagcaaa ttctgtggca gtggatgtta ttcctcatgt tgacaaccca
     1441 acctttgaag aagacgaaac tcctaatcaa gagactgctg ttcgagaaat taaatcttaa
     1501 aatctgtgta aatagaaaac ttgaaccatt agtaataaca gaactgccaa tcagggccta
     1561 gtttctatta ataaattgga taaatttaat aaaataagag tgatactgaa agtgctcaga
     1621 tgactaatat tatgctatag ttaaatggct taaaatattt aacctgttaa cttttttcca
     1681 caaactcatt ataatatttt tcataggcaa gtttcctctc agtagtgata acaacatttt
     1741 tagacattca aaactgtctt caagaagtca cgtttttcat ttataacaat tttcttataa
     1801 aaacatgttg cttttaaaat gtggagtagc tgtaatcact ttattttatg atagtatctt
     1861 aatgaaaaat actacttctt tagcttgggc tacatgtgtc agggtttttc tccaggtgct
     1921 tatattgatc tggaattgta atgtaaaaag caatgcaaac ttaggcgagt acttcttgaa
     1981 atgtctattt aagctgcttt aagttaatag aaaagattaa agcaaaatat tcatttttac
     2041 tttttcttat ttttaaaatt aggctgaatg tacttcatgt gatttgtcaa ccatagttta
     2101 tcagagatta tggacttaat tgattggtat attagtgaca tcaacttgac acaagattag
     2161 acaaaaaatt ccttacaaaa atactgtgta actatttctc aaacttgtgg gatttttcaa
     2221 aagctcagta tatgaatcat catactgttt gaaattgcta atgacagagt aagtaacact
     2281 aatattggtc attgatcttc gttcatgaat tagtctacag aaaaaaaatg ttctgtaaaa
     2341 ttagtctgtt gaaaatgttt tccaaacaat gttactttga aaattgagtt tatgtttgac
     2401 ctaaatgggc taaaattaca ttagataaac taaaattctg tccgtgtaac tataaatttt
     2461 gtgaatgcat tttcctggtg tttgaaaaag aaggggggga gaattccagg tgccttaata
     2521 taaagtttga agcttcatcc accaaagtta aatagagcta tttaaaaatg cactttattt
     2581 gtactctgtg tggcttttgt tttagaattt tgttcaaatt atagcagaat ttaggcaaaa
     2641 ataaaacaga catgtatttt tgtttgctga atggatgaaa ccattgcatt cttgtacact
     2701 gatttgaaat gctgtaaata tgtcccaatt tgtattgatt ctctttaaat ataaaatgta
     2761 aataaaatat tccaataaaa gtttgtgtct ggtgttagtt taa
//
