LOCUS       NM_018320               2282 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens ring finger protein 121 (RNF121), transcript variant
            1, mRNA.
ACCESSION   NM_018320
VERSION     NM_018320.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Xiang,P., Sun,Y., Liu,Y., Shu,Q. and Zhu,Y.
  TITLE     Really interesting new gene finger protein 121 is a tumor
            suppressor of renal cell carcinoma
  JOURNAL   Gene 676, 322-328 (2018)
   PUBMED   30149063
  REMARK    GeneRIF: Study found that RNF121 was less expressed in tumor
            tissues than adjacent normal tissues of renal cell carcinoma (RCC)
            patients. Overexpression of RNF121 inhibited the growth of human
            RCC cells in vivo. Moreover, RNF121 impeded the proliferation,
            migration and invasion of human RCC cells in vitro. In addition,
            RNF121 was found to activate NF-kappaB signaling pathway via
            promoting IkappaBalpha degradation in RCC cells.
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Choi,Y., Kwon,C.H., Lee,S.J., Park,J., Shin,J.Y. and Park,D.Y.
  TITLE     Integrative analysis of oncogenic fusion genes and their functional
            impact in colorectal cancer
  JOURNAL   Br J Cancer 119 (2), 230-240 (2018)
   PUBMED   29955133
  REMARK    GeneRIF: we identified and characterised oncogenic fusion genes and
            their function in CRC, and implicated NAGLU-IKZF3 and RNF121-FOLR2
            as novel molecular targets for personalised medicine development.
REFERENCE   3  (bases 1 to 2282)
  AUTHORS   Maghsoudlou,A., Meyer,R.D., Rezazadeh,K., Arafa,E., Pudney,J.,
            Hartsough,E. and Rahimi,N.
  TITLE     RNF121 Inhibits Angiogenic Growth Factor Signaling by Restricting
            Cell Surface Expression of VEGFR-2
  JOURNAL   Traffic 17 (3), 289-300 (2016)
   PUBMED   26602861
  REMARK    GeneRIF: Over-expression of RNF121 promoted ubiquitination of
            VEGFR-2, inhibited its maturation .  RNF121 knockdown in primary
            endothelial cells reduced VEGFR-2 ubiquitination and increased its
            cell surface level.
REFERENCE   4  (bases 1 to 2282)
  AUTHORS   Zemirli,N., Pourcelot,M., Dogan,N., Vazquez,A. and Arnoult,D.
  TITLE     The E3 ubiquitin ligase RNF121 is a positive regulator of NF-kappaB
            activation
  JOURNAL   Cell Commun Signal 12, 72 (2014)
   PUBMED   25388546
  REMARK    GeneRIF: These results unveil an unexpected role of Golgi Apparatus
            and reveal RNF121 as a new player involved in the signaling leading
            to NF-kappaB activation.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2282)
  AUTHORS   Zhao,Y., Hongdu,B., Ma,D. and Chen,Y.
  TITLE     Really interesting new gene finger protein 121 is a novel
            Golgi-localized membrane protein that regulates apoptosis
  JOURNAL   Acta Biochim Biophys Sin (Shanghai) 46 (8), 668-674 (2014)
   PUBMED   24928685
  REMARK    GeneRIF: This is the first study to demonstrate that RNF121 is a
            novel regulator of apoptosis and provides a new potential target
            for cancer therapy
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA209781.1, AP002490.4,
            AK023139.1, CN392009.1, AI291325.1 and AL833638.1.
            
            On Nov 22, 2018 this sequence version replaced NM_018320.4.
            
            Summary: The protein encoded by this gene contains a RING finger, a
            motif present in a variety of functionally distinct proteins and
            known to be involved in protein-protein and protein-DNA
            interactions. Several alternatively spliced transcript variants
            have been noted for this gene, however, not all are likely to
            encode viable protein products. [provided by RefSeq, Sep 2008].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (a).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.215247.1,
                                           SRR1803615.131982.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000361756.8/ ENSP00000354571.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-219               DA209781.1         346-564
            220-259             AP002490.4         50076-50115
            260-414             AP002490.4         71985-72139
            415-522             AP002490.4         76228-76335
            523-643             AP002490.4         79821-79941
            644-777             AP002490.4         83943-84076
            778-879             AP002490.4         84674-84775
            880-1172            AP002490.4         85419-85711
            1173-1651           AK023139.1         1163-1641
            1652-1838           CN392009.1         401-587
            1839-2242           AI291325.1         38-441              c
            2243-2282           AL833638.1         2078-2117
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q13.4"
     gene            1..2282
                     /gene="RNF121"
                     /note="ring finger protein 121"
                     /db_xref="GeneID:55298"
                     /db_xref="HGNC:HGNC:21070"
                     /db_xref="MIM:620529"
     exon            1..79
                     /gene="RNF121"
                     /inference="alignment:Splign:2.1.0"
     CDS             17..1000
                     /gene="RNF121"
                     /EC_number="2.3.2.27"
                     /note="isoform a is encoded by transcript variant 1; E3
                     ubiquitin ligase RNF121"
                     /codon_start=1
                     /product="E3 ubiquitin ligase RNF121 isoform a"
                     /protein_id="NP_060790.2"
                     /db_xref="CCDS:CCDS8203.1"
                     /db_xref="GeneID:55298"
                     /db_xref="HGNC:HGNC:21070"
                     /db_xref="MIM:620529"
                     /translation="MAAVVEVEVGGGAAGERELDEVDMSDLSPEEQWRVEHARMHAKH
                     RGHEAMHAEMVLILIATLVVAQLLLVQWKQRHPRSYNMVTLFQMWVVPLYFTVKLHWW
                     RFLVIWILFSAVTAFVTFRATRKPLVQTTPRLVYKWFLLIYKISYATGIVGYMAVMFT
                     LFGLNLLFKIKPEDAMDFGISLLFYGLYYGVLERDFAEMCADYMASTIGFYSESGMPT
                     KHLSDSVCAVCGQQIFVDVSEEGIIENTYRLSCNHVFHEFCIRGWCIVGKKQTCPYCK
                     EKVDLKRMFSNPWERPHVMYGQLLDWLRYLVAWQPVIIGVVQGINYILGLE"
     exon            80..117
                     /gene="RNF121"
                     /inference="alignment:Splign:2.1.0"
     exon            118..259
                     /gene="RNF121"
                     /inference="alignment:Splign:2.1.0"
     exon            260..414
                     /gene="RNF121"
                     /inference="alignment:Splign:2.1.0"
     exon            415..522
                     /gene="RNF121"
                     /inference="alignment:Splign:2.1.0"
     exon            523..643
                     /gene="RNF121"
                     /inference="alignment:Splign:2.1.0"
     exon            644..777
                     /gene="RNF121"
                     /inference="alignment:Splign:2.1.0"
     exon            778..879
                     /gene="RNF121"
                     /inference="alignment:Splign:2.1.0"
     exon            880..2282
                     /gene="RNF121"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2249..2254
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RNF121"
                     /note="hexamer: AATAAA"
     polyA_site      2282
                     /gene="RNF121"
                     /note="major polyA site"
ORIGIN      
        1 ggggcgagcc gtgaagatgg cggcagtggt ggaggtggag gttggaggtg gtgctgctgg
       61 ggaacgggag ctggatgagg ttgatatgtc agatctctct ccagaagagc aatggagggt
      121 cgagcacgca cgcatgcatg ccaagcaccg tggccatgaa gctatgcatg ctgaaatggt
      181 cctcatcctc atcgcaacct tggtggtggc ccagctgctc ctggtgcagt ggaagcagag
      241 gcacccacgc tcctacaata tggtgaccct ctttcagatg tgggttgttc ccctctattt
      301 cacagtgaag ctgcactggt ggaggttcct agtgatctgg atcttgttct ctgctgtcac
      361 agcctttgtt accttccgag ccacccgaaa acctctagta cagacaaccc caaggttggt
      421 ttataagtgg ttcctgctaa tctataaaat cagctatgcc actggcattg ttggctacat
      481 ggctgtcatg tttaccctct ttggtcttaa cttattattc aagatcaaac cagaagatgc
      541 catggacttt ggcatctccc ttctcttcta tggcctctac tatggagttc tggaacggga
      601 ctttgcagaa atgtgtgcag actacatggc atctaccata gggttctaca gcgagtcggg
      661 catgcctacc aaacatcttt cagacagtgt gtgtgctgtg tgtgggcagc agatctttgt
      721 ggacgtcagt gaagagggga tcattgagaa cacgtatagg ctgtcctgca atcatgtctt
      781 ccacgagttc tgcatccgtg gctggtgcat cgtgggaaag aagcaaacgt gtccctactg
      841 caaagagaag gtagacctca agaggatgtt cagcaatccc tgggagaggc ctcacgtcat
      901 gtatgggcaa ctgctggact ggcttcgata cttggtagcc tggcagcctg tcatcattgg
      961 tgtagtccaa ggcatcaact acatcctggg cctggaatag tgatgaagag catcagtgga
     1021 aaacccaccc cacacgccat ggacctcagg gcactctcct ccctgcccac aaagacctcc
     1081 tgggtgggaa agactcaaag gggtgcttgg gccactcagg acccctctgg ctgtgtcgga
     1141 ctggggaggg atatgatgga gagccagcca gtggggctgt cagcagtggg gggcttttta
     1201 aaagaaaact attttgatga atatatttaa aaaacctttt tttattgtgg agcataggaa
     1261 ttgcccccct ccaggcttca ccctccctgc ctaagcaggg tgggggcaga gccatgacat
     1321 ttttggttta aaggagcctt ctcatctctg gccgagaaca ctgctgggct cccaggtagc
     1381 tgaaggcctc agcccaccca ctcccttctt ccctgtgtgg ggctcaagcc tggtgcactt
     1441 agtatagaag agctaacgta ctcaggcttg tgccacgggt gagcactgag gccccaggtg
     1501 tcctccctcc cccactgtct gggacacagg acaggtgttg cgtctgactc tgtctccccc
     1561 tgtccaagag ctataggctg gttgaggcaa gtggaacctt atcagtgact tggccaacaa
     1621 gggaagcctt ggggcctcag aattcattga agatttgttt tgggacaact ggaagaatca
     1681 gcaccatgga tactcgcccg tgggcagggc tgtggtcagc caagaataga ggtcatatag
     1741 ttgggccatg gggcaattgt tggcagccat gactgacggc cccttgtgca agctcattct
     1801 tcagtggatc cttcagtgga gtggtgacac gtgccagcgg tcactctgcc acatcacttc
     1861 cttcttgaaa gcctaagagt gcgtatgcgt gtgtgtgtgt gtgtgtgtgt gtacgtgagg
     1921 cagaagaata tgacagatgt cagcttctcc ctgtgtcaga tgatatgtcc ttgaagctgg
     1981 caggggattc ccagtccagg gaatatccct acatgatttt tgtcattcat gggtcctccc
     2041 tgggcctgac aacgggagtg gtgggtgggt gagaacaggt tctgagaggg ggcttctagg
     2101 aaggccccag ccctagtcct aggcgacttg aataaagtgg gaggtcaaac atgggccttc
     2161 ggcattgggt ttctagggaa tatggctcag tagggcctgc cccttcaagg gcagcaagaa
     2221 cgaagcagga tgttgtcact cccgtctgaa taaagctcca tgagctgggt tggaaagctg
     2281 aa
//
