LOCUS       NM_017610               5905 bp    mRNA    linear   PRI 10-FEB-2025
DEFINITION  Homo sapiens ring finger protein 111 (RNF111), transcript variant
            2, mRNA.
ACCESSION   NM_017610
VERSION     NM_017610.8
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Song,C., Qin,Y., Li,Y., Yang,B., Guo,T., Ma,W., Xu,D., Xu,K.,
            Fu,F., Jin,L., Wu,Y., Tang,S., Chen,X. and Zhang,F.
  TITLE     Deleterious variants in RNF111 impair female fertility and induce
            premature ovarian insufficiency in humans and mice
  JOURNAL   Sci China Life Sci 67 (7), 1325-1337 (2024)
   PUBMED   38874713
  REMARK    GeneRIF: Deleterious variants in RNF111 impair female fertility and
            induce premature ovarian insufficiency in humans and mice.
REFERENCE   2  (bases 1 to 5905)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 5905)
  AUTHORS   Laigle,V., Dingli,F., Amhaz,S., Perron,T., Chouchene,M.,
            Colasse,S., Petit,I., Poullet,P., Loew,D., Prunier,C. and Levy,L.
  TITLE     Quantitative Ubiquitylome Analysis Reveals the Specificity of
            RNF111/Arkadia E3 Ubiquitin Ligase for its Degradative Substrates
            SKI and SKIL/SnoN in TGF-beta Signaling Pathway
  JOURNAL   Mol Cell Proteomics 20, 100173 (2021)
   PUBMED   34740826
  REMARK    GeneRIF: Quantitative Ubiquitylome Analysis Reveals the Specificity
            of RNF111/Arkadia E3 Ubiquitin Ligase for its Degradative
            Substrates SKI and SKIL/SnoN in TGF-beta Signaling Pathway.
REFERENCE   4  (bases 1 to 5905)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 5905)
  AUTHORS   Buljan,M., Ciuffa,R., van Drogen,A., Vichalkovski,A., Mehnert,M.,
            Rosenberger,G., Lee,S., Varjosalo,M., Pernas,L.E., Spegg,V.,
            Snijder,B., Aebersold,R. and Gstaiger,M.
  TITLE     Kinase Interaction Network Expands Functional and Disease Roles of
            Human Kinases
  JOURNAL   Mol Cell 79 (3), 504-520 (2020)
   PUBMED   32707033
REFERENCE   6  (bases 1 to 5905)
  AUTHORS   Colland,F., Jacq,X., Trouplin,V., Mougin,C., Groizeleau,C.,
            Hamburger,A., Meil,A., Wojcik,J., Legrain,P. and Gauthier,J.M.
  TITLE     Functional proteomics mapping of a human signaling pathway
  JOURNAL   Genome Res 14 (7), 1324-1332 (2004)
   PUBMED   15231748
REFERENCE   7  (bases 1 to 5905)
  AUTHORS   Koinuma,D., Shinozaki,M., Komuro,A., Goto,K., Saitoh,M., Hanyu,A.,
            Ebina,M., Nukiwa,T., Miyazawa,K., Imamura,T. and Miyazono,K.
  TITLE     Arkadia amplifies TGF-beta superfamily signalling through
            degradation of Smad7
  JOURNAL   EMBO J 22 (24), 6458-6470 (2003)
   PUBMED   14657019
  REMARK    GeneRIF: Silencing of the Arkadia gene resulted in repression of
            transcriptional activities induced by TGF-beta and BMP, and
            accumulation of the Smad7 protein.
REFERENCE   8  (bases 1 to 5905)
  AUTHORS   Patten,I. and Placzek,M.
  TITLE     Vertebrate development: Et in Arkadia
  JOURNAL   Curr Biol 11 (15), R616-R619 (2001)
   PUBMED   11516970
  REMARK    Review article
REFERENCE   9  (bases 1 to 5905)
  AUTHORS   Niederlander,C., Walsh,J.J., Episkopou,V. and Jones,C.M.
  TITLE     Arkadia enhances nodal-related signalling to induce mesendoderm
  JOURNAL   Nature 410 (6830), 830-834 (2001)
   PUBMED   11298453
  REMARK    GeneRIF: Functional analysis of the mouse counterpart
REFERENCE   10 (bases 1 to 5905)
  AUTHORS   Episkopou,V., Arkell,R., Timmons,P.M., Walsh,J.J., Andrew,R.L. and
            Swan,D.
  TITLE     Induction of the mammalian node requires Arkadia function in the
            extraembryonic lineages
  JOURNAL   Nature 410 (6830), 825-830 (2001)
   PUBMED   11298452
  REMARK    GeneRIF: Functional analysis of the mouse counterpart
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC025918.7, BX538130.1,
            CN420669.1, AL832089.1, AL157474.1, BX647259.1, N24855.1,
            BM986870.1 and AI189975.1.
            
            On Jun 2, 2019 this sequence version replaced NM_017610.7.
            
            Summary: The protein encoded by this gene is a nuclear RING-domain
            containing E3 ubiquitin ligase. This protein interacts with the
            transforming growth factor (TGF) -beta/NODAL signaling pathway by
            promoting the ubiquitination and proteosomal degradation of
            negative regulators, like SMAD proteins, and thereby enhances
            TGF-beta target-gene transcription. As a modulator of the nodal
            signaling cascade, this gene plays a critical role in the induction
            of mesoderm during embryonic development. Alternative splicing of
            this gene results in multiple transcript variants encoding distinct
            isoforms. [provided by RefSeq, Jul 2012].
            
            Transcript Variant: This variant (2) lacks an in-frame segment in
            the 3' coding region, compared to variant 1, which results in a
            shorter protein (isoform 2), compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.98011.1,
                                           SRR1803616.195225.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000348370.9/ ENSP00000288199.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 AC025918.7         169403-169405
            4-451               BX538130.1         2-449
            452-452             CN420669.1         155-155
            453-1842            BX538130.1         451-1840
            1843-3164           AL832089.1         425-1746
            3165-4912           AL157474.1         389-2136
            4913-5305           BX647259.1         4651-5043
            5306-5641           N24855.1           5-340               c
            5642-5813           BM986870.1         23-194              c
            5814-5905           AI189975.1         21-112              c
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q22.1-q22.2"
     gene            1..5905
                     /gene="RNF111"
                     /gene_synonym="ARK; hRNF111"
                     /note="ring finger protein 111"
                     /db_xref="GeneID:54778"
                     /db_xref="HGNC:HGNC:17384"
                     /db_xref="MIM:605840"
     exon            1..406
                     /gene="RNF111"
                     /gene_synonym="ARK; hRNF111"
                     /inference="alignment:Splign:2.1.0"
     exon            407..1305
                     /gene="RNF111"
                     /gene_synonym="ARK; hRNF111"
                     /inference="alignment:Splign:2.1.0"
     CDS             426..3386
                     /gene="RNF111"
                     /gene_synonym="ARK; hRNF111"
                     /EC_number="2.3.2.27"
                     /note="isoform 2 is encoded by transcript variant 2;
                     Arkadia; E3 ubiquitin-protein ligase Arkadia; RING-type E3
                     ubiquitin transferase Arkadia"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase Arkadia isoform 2"
                     /protein_id="NP_060080.6"
                     /db_xref="CCDS:CCDS10169.1"
                     /db_xref="GeneID:54778"
                     /db_xref="HGNC:HGNC:17384"
                     /db_xref="MIM:605840"
                     /translation="MSQWTPEYNELYTLKVDMKSEIPSDAPKTQESLKGILLHPEPIG
                     AAKSFPAGVEMINSKVGNEFSHLCDDSQKQEKEMNGNQQEQEKSLVVRKKRKSQQAGP
                     SYVQNCVKENQGILGLRQHLGTPSDEDNDSSFSDCLSSPSSSLHFGDSDTVTSDEDKE
                     VSVRHSQTILNAKSRSHSARSHKWPRTETESVSGLLMKRPCLHGSSLRRLPCRKRFVK
                     NNSSQRTQKQKERILMQRKKREVLARRKYALLPSSSSSSENDLSSESSSSSSTEGEED
                     LFVSASENHQNNPAVPSGSIDEDVVVIEASSTPQVTANEEINVTSTDSEVEIVTVGES
                     YRSRSTLGHSRSHWSQGSSSHASRPQEPRNRSRISTVIQPLRQNAAEVVDLTVDEDEP
                     TVVPTTSARMESQATSASINNSNPSTSEQASDTASAVTSSQPSTVSETSATLTSNSTT
                     GTSIGDDSRRTTSSAVTETGPPAMPRLPSCCPQHSPCGGSSQNHHALGHPHTSCFQQH
                     GHHFQHHHHHHHTPHPAVPVSPSFSDPACPVERPPQVQAPCGANSSSGTSYHEQQALP
                     VDLSNSGIRSHGSGSFHGASAFDPCCPVSSSRAAIFGHQAAAAAPSQPLSSIDGYGSS
                     MVAQPQPQPPPQPSLSSCRHYMPPPYASLTRPLHHQASACPHSHGNPPPQTQPPPQVD
                     YVIPHPVHAFHSQISSHATSHPVAPPPPTHLASTAAPIPQHLPPTHQPISHHIPATAP
                     PAQRLHPHEVMQRMEVQRRRMMQHPTRAHERPPPHPHRMHPNYGHGHHIHVPQTMSSH
                     PRQAPERSAWELGIEAGVTAATYTPGALHPHLAHYHAPPRLHHLQLGALPLMVPDMAG
                     YPHIRYISSGLDGTSFRGPFRGNFEELIHLEERLGNVNRGASQGTIERCTYPHKYKKR
                     KLHCKQDGEEGTEEDTEEKCTICLSILEEGEDVRRLPCMHLFHQVCVDQWLITNKKCP
                     ICRVDIEAQLPSES"
     exon            1306..1432
                     /gene="RNF111"
                     /gene_synonym="ARK; hRNF111"
                     /inference="alignment:Splign:2.1.0"
     exon            1433..1596
                     /gene="RNF111"
                     /gene_synonym="ARK; hRNF111"
                     /inference="alignment:Splign:2.1.0"
     exon            1597..1791
                     /gene="RNF111"
                     /gene_synonym="ARK; hRNF111"
                     /inference="alignment:Splign:2.1.0"
     exon            1792..2111
                     /gene="RNF111"
                     /gene_synonym="ARK; hRNF111"
                     /inference="alignment:Splign:2.1.0"
     exon            2112..2373
                     /gene="RNF111"
                     /gene_synonym="ARK; hRNF111"
                     /inference="alignment:Splign:2.1.0"
     exon            2374..2722
                     /gene="RNF111"
                     /gene_synonym="ARK; hRNF111"
                     /inference="alignment:Splign:2.1.0"
     exon            2723..2848
                     /gene="RNF111"
                     /gene_synonym="ARK; hRNF111"
                     /inference="alignment:Splign:2.1.0"
     exon            2849..2975
                     /gene="RNF111"
                     /gene_synonym="ARK; hRNF111"
                     /inference="alignment:Splign:2.1.0"
     exon            2976..3068
                     /gene="RNF111"
                     /gene_synonym="ARK; hRNF111"
                     /inference="alignment:Splign:2.1.0"
     exon            3069..3164
                     /gene="RNF111"
                     /gene_synonym="ARK; hRNF111"
                     /inference="alignment:Splign:2.1.0"
     exon            3165..3268
                     /gene="RNF111"
                     /gene_synonym="ARK; hRNF111"
                     /inference="alignment:Splign:2.1.0"
     exon            3269..5905
                     /gene="RNF111"
                     /gene_synonym="ARK; hRNF111"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4892..4897
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RNF111"
                     /gene_synonym="ARK; hRNF111"
                     /note="hexamer: AATAAA"
     polyA_site      4917
                     /gene="RNF111"
                     /gene_synonym="ARK; hRNF111"
                     /note="major polyA site"
     regulatory      5519..5524
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RNF111"
                     /gene_synonym="ARK; hRNF111"
                     /note="hexamer: AATAAA"
     polyA_site      5541
                     /gene="RNF111"
                     /gene_synonym="ARK; hRNF111"
     regulatory      5793..5798
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RNF111"
                     /gene_synonym="ARK; hRNF111"
                     /note="hexamer: GATAAA"
     polyA_site      5816
                     /gene="RNF111"
                     /gene_synonym="ARK; hRNF111"
     regulatory      5882..5887
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RNF111"
                     /gene_synonym="ARK; hRNF111"
                     /note="hexamer: GATAAA"
     polyA_site      5905
                     /gene="RNF111"
                     /gene_synonym="ARK; hRNF111"
ORIGIN      
        1 agtgtcgtgc tctcctcggt aggggaggaa ttggttaggc ggcggcggcg gcgaagcggc
       61 ggcggcggct gtaggggagc agcggcagtg gcggcgacgg cgaggaggtg ttcggtttgc
      121 gcagcgtaga aacgcccgca gcttcagaga aggagttctt gtgggaccaa attaggcatt
      181 ctgaacccac gccggcttag ctgggccgag tccaggcgct gccgcacgta cagttttggt
      241 ggcggtgaca gacactgttc ttgactctag gggcctatta ggccgacgtc tgaggcgcag
      301 atcgctggct ctcgacgcat tactctttac gccttacatt tctgtcttcc ttcctgggtc
      361 agtgattccc ggaccctgga agagaagagg gtggctaatg attaaggctt tccttaaagt
      421 ttcccatgtc tcaatggact cctgaatata acgagctcta caccttaaaa gtggatatga
      481 agagtgagat tccttctgat gcaccaaaga cacaggagag tctgaaaggg atccttttgc
      541 atccagagcc cattggggca gccaaaagtt ttcctgcagg agttgagatg attaatagta
      601 aagtggggaa tgaattctct cacctgtgtg atgattctca aaagcaagag aaggaaatga
      661 atggtaacca gcaagaacaa gaaaaaagtc tcgttgtgag gaaaaaacgc aaaagccagc
      721 aggctggccc ttcgtatgtg cagaattgtg ttaaagaaaa ccagggaata ttaggactga
      781 ggcaacacct agggacacca agtgatgaag ataatgattc ctcttttagt gattgtcttt
      841 cttctccttc atctagtctg cattttggag attctgatac tgtgacttca gatgaggata
      901 aagaagtctc tgtaagacat tcccagacca ttttgaatgc taaaagtaga agccatagtg
      961 cacggtctca taagtggcct cggactgaga cagaatctgt atcgggattg ttaatgaaaa
     1021 gaccctgttt acatggcagt tcgttacgga gacttccatg cagaaagaga tttgtaaaaa
     1081 ataattcctc acagaggaca cagaaacaaa aagagaggat attaatgcag aggaagaaac
     1141 gagaagtgtt agctcgaaga aaatatgcct tgctacctag ttctagtagt tccagtgaga
     1201 atgacctcag cagtgaatcc tcttctagct catcaactga aggagaagaa gatttgtttg
     1261 tttctgccag tgaaaaccac caaaacaatc cagctgttcc ctcaggaagt attgatgaag
     1321 atgttgtggt gatagaagct tcctccactc cccaggttac tgccaatgaa gaaattaatg
     1381 ttacctcaac tgacagtgaa gtggagattg taacagttgg agaaagctat cggtctcgtt
     1441 caacccttgg acactccaga tctcattgga gccagggttc cagttctcat gcaagtcggc
     1501 cacaggagcc acggaaccgc agtaggattt ctactgttat acagcccttg aggcagaatg
     1561 cagcagaagt tgtggacctt accgttgatg aagatgaacc tactgtagta ccaaccactt
     1621 ctgcaagaat ggaatcacaa gctactagcg cttccattaa caattcaaat ccatctacct
     1681 ctgagcaggc ctctgatact gcttcagctg tcaccagtag ccaaccttcc acagtgtcag
     1741 agacttcagc tactcttaca agcaatagta ccactggcac ttctatagga gatgactcaa
     1801 ggagaactac atctagtgct gtaacggaaa ctggccctcc tgcaatgcca aggttacctt
     1861 cctgctgtcc ccagcactca ccatgtggag ggtcgtcaca gaaccaccat gcattaggac
     1921 atcctcatac aagttgcttt cagcagcatg gtcaccattt tcaacatcat caccaccacc
     1981 accatactcc ccacccagct gtcccagttt ctccttcctt tagtgatcct gcttgccctg
     2041 tggaaagacc tccacaagta caagcacctt gtggagcaaa tagtagttct ggtaccagct
     2101 atcatgaaca gcaggcattg ccagtggacc tgagcaacag tggtatcaga agtcatggaa
     2161 gtggcagttt tcatggagca tctgcatttg acccctgctg ccctgtttct tcctcccgag
     2221 ctgcaatctt tggccatcag gccgctgctg ctgccccaag tcaaccttta tcatcaatag
     2281 atggctatgg atcaagcatg gttgcgcagc cccagcccca gccccctcca cagccctctc
     2341 tctcatcatg tcgacattac atgccacccc cttatgcctc tttgacaagg ccacttcatc
     2401 atcaagcttc tgcctgcccg cattctcatg gaaacccccc tcctcagact cagcctccgc
     2461 ctcaagtgga ttatgttatt cctcatcctg tacatgcttt ccattctcaa atatcttctc
     2521 atgcaacatc tcatcctgtg gcacccccac caccaactca cttagccagt acagctgcac
     2581 caatccctca gcatcttcct cctacacacc agccaatttc gcaccatatt ccagccacag
     2641 cacctccagc acagagactg catcctcatg aagtgatgca gaggatggaa gttcaaagga
     2701 ggaggatgat gcagcatcca acgcgggcac atgaacgccc cccaccccat ccacatagga
     2761 tgcacccaaa ctatggtcat gggcatcata ttcatgtgcc tcagactatg tcctcacatc
     2821 ctcgacaggc tccagagagg tctgcctggg aactgggaat tgaagctgga gtgactgcag
     2881 ctacttatac acctggtgca ttgcatcctc acttggccca ttatcacgca cctcctcgac
     2941 ttcatcactt acaattagga gctcttcctt taatggttcc tgatatggca ggctatcctc
     3001 acatccgtta catttcatca ggattggatg gaacatcatt cagaggtcct ttcaggggca
     3061 attttgagga actgattcat ttggaagaaa gattaggcaa tgtcaatcgt ggagcatccc
     3121 aggggacaat tgaaagatgt acatatccac ataaatacaa aaagaggaaa ctgcactgca
     3181 aacaagatgg ggaagaaggg actgaggaag acacagagga aaaatgtact atctgtttgt
     3241 ctattttaga ggaaggtgaa gatgtgagac gtcttccatg tatgcacctt ttccaccaag
     3301 tgtgtgttga ccaatggttg attaccaata agaagtgccc catatgcaga gtggacattg
     3361 aggcccagct gccaagtgaa agttgacacc atgtttcaga actcttgccc tccctctcat
     3421 tcccatcctt cctggtactg cagtcaacca aagatggcat gacttacctg cgcagatttg
     3481 gaagcattga acttagagtg ctggctctgc tatatggtac aactaatgct agacctacag
     3541 tttatgtata cagttgattt tgatgtattt ataaaagctt ttttttctag atttgacatt
     3601 tttctgtatc attttactgt atttttgcat ggttccttgt attgcatttc tttgcacata
     3661 ttatgggctt gtgaccctaa acttgcaggc aaggttagct gctttagtaa gtagaatttt
     3721 gtggtctttt tgttttttac atagtaccaa gccttgataa ttatgaattt tttatccatt
     3781 actaaccttt aatttaatca atcatgtact ttagtttaat gtataaagat cctctagaaa
     3841 atgataatat tgtgtattaa gacattcctt aattaggaca aaatggctgc tgtatattta
     3901 ctatatggag ttctgagtta aataccatcc ttaatactgg gaacagaata caacccatat
     3961 aaatcagatg caggtggtag tcacatcacc agagtgatca gtataaattt tcttggtgta
     4021 tccttttcct ttcaacacag tgcagataag agttgaatat tgatatcata catttagact
     4081 gctgttctga ttgcatttat ctttttccta catcatttag aattttattt ccctgattca
     4141 gtttttgctg ctgtgaaaca gctctgatga acactaaata ttaatttcaa ttagctagat
     4201 tgtacatact tgcagattta acaaaatttt agggaaattg aaaaagacat gtagaatttg
     4261 ttgtcttctg ctaagcacga aaagttaaga tatctgctta cattgatttt gtagacacat
     4321 taagtcaaga tttggaattt aagtcactgg caggtatctg tgcattcata gaacttataa
     4381 aggtcccagg atcactttta agggattttt attagtttaa aggtaaataa agtcagctga
     4441 atctacatgt ctcttgtttt atttctctct aaacttgaaa acagtaaatc tgcagatact
     4501 gtgaggcaca aattatactg tcaacctact gttgctatgg ttatatactc ccacttcata
     4561 cattaccaag agtcgatcac tgatttaaaa tttttaattt ctatagttaa gatttactgc
     4621 ataatataga atataaagtt aagttaacat actaacattt ctcctttgga ggaagtttta
     4681 atctacttca ggatgcatat tattatcaag atactttcat atacaggata gcctaatttt
     4741 atttgtttaa atatgcttaa tatgccccag attgcaaatg catccagtca gtaatatcac
     4801 tgtctgtatg tggaggacat gttcccatgg atcatatgtg aagatgtcaa taagcttgca
     4861 ttaagccacc tgctttgtaa gtggattgat taataaataa cttatatttc tattgtcttt
     4921 gtgtttcata attagttttt ataaaaacag tttacattaa acatcttgga attcaacaat
     4981 agtgatctca ctcaaattga catttgcata atttgacatt tatattcatt cagttatgac
     5041 tacttgccct tttcttcata ttagtaaatg ttacactgta gtagttgtca atgttaacag
     5101 gcttcaacat agtatgtgtg cagatggacg atggatttaa agagcatacc ttatgattaa
     5161 accgaggtta tagaaggtca ggggagagtg aaggagtcag tgacctatgt ctgccatctt
     5221 actggggaaa agagcaagta gcctgtcttc ctttaagtaa gtagcctgta atggatgtac
     5281 tactttgcca ctggaatacc ccgggtctgt gccaagggac tgaagaagtg taagatgggg
     5341 agaactgtat gtcatggagt cctcttgacg ttcccccaga tacaagtaaa gcacaggtgg
     5401 atttctctat ttttgaatac tacatcagat ttagatgcac gactacctgt tgacttgttt
     5461 tataattgct actgattttt ttaatgtagg caaagctttg ttactttgaa atttatttaa
     5521 taaaagtatt tgtgacataa acctgaattg ttaatatagg agcttattaa gctactgcca
     5581 ttagttatcg aaaaatgtga taagtaatga agaaagtaag gaagaaaatg actttgaaca
     5641 ttttgacttt ttgtgctttg ttttggtgtc ttccatatcc tgctgcatct tatatgtcaa
     5701 aatgaacatt tcagtggtag ctgtccaaat acataaaaac taaaattctt ttgttagcaa
     5761 gtccttattt ttattgttgt taacatgcaa gtgataaact gataatttga aacatttttc
     5821 ttactctggt gactttcttt tagctgctag cacacttcag catttgaacc ataacagttg
     5881 tgataaactg atcgtttgaa acgtt
//
