LOCUS       NM_002937               2061 bp    mRNA    linear   PRI 12-AUG-2024
DEFINITION  Homo sapiens ribonuclease A family member 4 (RNASE4), transcript
            variant 2, mRNA.
ACCESSION   NM_002937
VERSION     NM_002937.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Sun,J., Chen,M., Hu,Z., Xu,N., Wang,W., Ping,Z., Zhu,J., Sun,D.,
            Zhu,Z., Li,H., Ge,X., Luo,L., Zhou,W., Bai,R., Xu,Z. and Sheng,J.
  TITLE     Ribonuclease 4 functions as an intestinal antimicrobial protein to
            maintain gut microbiota and metabolite homeostasis
  JOURNAL   Nat Commun 15 (1), 5778 (2024)
   PUBMED   38987259
  REMARK    GeneRIF: Ribonuclease 4 functions as an intestinal antimicrobial
            protein to maintain gut microbiota and metabolite homeostasis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Vanli,N., Sheng,J., Li,S., Xu,Z. and Hu,G.F.
  TITLE     Ribonuclease 4 is associated with aggressiveness and progression of
            prostate cancer
  JOURNAL   Commun Biol 5 (1), 625 (2022)
   PUBMED   35752711
  REMARK    GeneRIF: Ribonuclease 4 is associated with aggressiveness and
            progression of prostate cancer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2061)
  AUTHORS   Bender,K., Schwartz,L.L., Cohen,A., Vasquez,C.M., Murtha,M.J.,
            Eichler,T., Thomas,J.P., Jackson,A. and Spencer,J.D.
  TITLE     Expression and function of human ribonuclease 4 in the kidney and
            urinary tract
  JOURNAL   Am J Physiol Renal Physiol 320 (5), F972-F983 (2021)
   PUBMED   33818125
  REMARK    GeneRIF: Expression and function of human ribonuclease 4 in the
            kidney and urinary tract.
REFERENCE   4  (bases 1 to 2061)
  AUTHORS   Padhi,A.K. and Gomes,J.
  TITLE     A molecular dynamics based investigation reveals the role of rare
            Ribonuclease 4 variants in amyotrophic lateral sclerosis
            susceptibility
  JOURNAL   Mutat Res 813, 1-12 (2019)
   PUBMED   30544007
  REMARK    GeneRIF: this is the first study to demonstrate that although rare
            and not yet clinically correlated, certain rare RNASE4 variants
            could cause ALS due to their loss-of-function characteristics and
            highlights the need to discover novel RNASE variants for a
            comprehensive understanding of structure-function-disease
            relationships and design effective therapeutics
REFERENCE   5  (bases 1 to 2061)
  AUTHORS   Dong,X. and Cong,S.
  TITLE     Identification of differentially expressed genes and regulatory
            relationships in Huntington's disease by bioinformatics analysis
  JOURNAL   Mol Med Rep 17 (3), 4317-4326 (2018)
   PUBMED   29328442
  REMARK    GeneRIF: RNASE4, STAT3, and miRNA-124 may have a regulatory
            association with the pathological mechanisms in Huntington's
            disease.
REFERENCE   6  (bases 1 to 2061)
  AUTHORS   Egesten,A., Dyer,K.D., Batten,D., Domachowske,J.B. and
            Rosenberg,H.F.
  TITLE     Ribonucleases and host defense: identification, localization and
            gene expression in adherent monocytes in vitro
  JOURNAL   Biochim Biophys Acta 1358 (3), 255-260 (1997)
   PUBMED   9366257
REFERENCE   7  (bases 1 to 2061)
  AUTHORS   Rosenberg,H.F. and Dyer,K.D.
  TITLE     Human ribonuclease 4 (RNase 4): coding sequence, chromosomal
            localization and identification of two distinct transcripts in
            human somatic tissues
  JOURNAL   Nucleic Acids Res 23 (21), 4290-4295 (1995)
   PUBMED   7501448
REFERENCE   8  (bases 1 to 2061)
  AUTHORS   Seno,M., Futami,J., Tsushima,Y., Akutagawa,K., Kosaka,M., Tada,H.
            and Yamada,H.
  TITLE     Molecular cloning and expression of human ribonuclease 4 cDNA
  JOURNAL   Biochim Biophys Acta 1261 (3), 424-426 (1995)
   PUBMED   7742370
REFERENCE   9  (bases 1 to 2061)
  AUTHORS   Zhou,H.M. and Strydom,D.J.
  TITLE     The amino acid sequence of human ribonuclease 4, a highly conserved
            ribonuclease that cleaves specifically on the 3' side of uridine
  JOURNAL   Eur J Biochem 217 (1), 401-410 (1993)
   PUBMED   8223579
REFERENCE   10 (bases 1 to 2061)
  AUTHORS   Shapiro,R., Fett,J.W., Strydom,D.J. and Vallee,B.L.
  TITLE     Isolation and characterization of a human colon carcinoma-secreted
            enzyme with pancreatic ribonuclease-like activity
  JOURNAL   Biochemistry 25 (23), 7255-7264 (1986)
   PUBMED   3467790
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK092422.1, CA447209.1 and
            AL163636.7.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_002937.4.
            
            Summary: The protein encoded by this gene belongs to the pancreatic
            ribonuclease family. It plays an important role in mRNA cleavage
            and has marked specificity towards the 3' side of uridine
            nucleotides. Alternative splicing results in four transcript
            variants encoding the same protein. This gene and the gene that
            encodes angiogenin share promoters and 5' exons. Each gene splices
            to a unique downstream exon that contains its complete coding
            region. [provided by RefSeq, Aug 2013].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 4. All four variants encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DA925415.1, SRR5189652.213916.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000555835.3/ ENSP00000452245.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-740               AK092422.1         348-1087
            741-1447            CA447209.1         15-721              c
            1448-2061           AL163636.7         132691-133304
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q11.2"
     gene            1..2061
                     /gene="RNASE4"
                     /gene_synonym="RAB1; RNS4"
                     /note="ribonuclease A family member 4"
                     /db_xref="GeneID:6038"
                     /db_xref="HGNC:HGNC:10047"
                     /db_xref="MIM:601030"
     exon            1..199
                     /gene="RNASE4"
                     /gene_synonym="RAB1; RNS4"
                     /inference="alignment:Splign:2.1.0"
     exon            200..2061
                     /gene="RNASE4"
                     /gene_synonym="RAB1; RNS4"
                     /inference="alignment:Splign:2.1.0"
     CDS             217..660
                     /gene="RNASE4"
                     /gene_synonym="RAB1; RNS4"
                     /EC_number="3.1.27.-"
                     /note="RNase 4; ribonuclease A B1; ribonuclease, RNase A
                     family, 4; epididymis secretory sperm binding protein"
                     /codon_start=1
                     /product="ribonuclease 4 precursor"
                     /protein_id="NP_002928.1"
                     /db_xref="CCDS:CCDS9555.1"
                     /db_xref="GeneID:6038"
                     /db_xref="HGNC:HGNC:10047"
                     /db_xref="MIM:601030"
                     /translation="MALQRTHSLLLLLLLTLLGLGLVQPSYGQDGMYQRFLRQHVHPE
                     ETGGSDRYCNLMMQRRKMTLYHCKRFNTFIHEDIWNIRSICSTTNIQCKNGKMNCHEG
                     VVKVTDCRDTGSSRAPNCRYRAIASTRRVVIACEGNPQVPVHFDG"
     regulatory      1416..1421
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RNASE4"
                     /gene_synonym="RAB1; RNS4"
                     /note="hexamer: AATAAA"
     polyA_site      1444
                     /gene="RNASE4"
                     /gene_synonym="RAB1; RNS4"
                     /note="major polyA site"
     regulatory      2035..2040
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RNASE4"
                     /gene_synonym="RAB1; RNS4"
                     /note="hexamer: ATTAAA"
     polyA_site      2061
                     /gene="RNASE4"
                     /gene_synonym="RAB1; RNS4"
ORIGIN      
        1 accacgcccc catctccgtc cgtgtacaca cactcacaca aggacgccaa ccccacctag
       61 atgcaaagca ggattcaaaa gaacatcttt gcgttttcta ccggctcccc atcatcgtac
      121 tagggaggaa gaagcgggtg agaaacaaaa cttctttcca ttgtcctgcc cgtttctgcg
      181 gacttgttct gaggccgagg cacctctaag atactgatgg ctctgcagag gacccattca
      241 ttgcttctgc ttttgctgct gaccctgctg gggctggggc tggtccagcc ctcctatggc
      301 caggatggca tgtaccagcg attcctgcgg caacacgtgc accctgagga gacaggtggc
      361 agtgatcgct actgcaactt gatgatgcaa agacggaaga tgactttgta tcactgcaag
      421 cgcttcaaca ccttcatcca tgaagatatc tggaacattc gtagtatctg cagcaccacc
      481 aatatccaat gcaagaacgg caagatgaac tgccatgagg gtgtagtgaa ggtcacagat
      541 tgcagggaca caggaagttc cagggcaccc aactgcagat atcgggccat agcgagcact
      601 agacgtgttg tcattgcctg tgagggtaac ccacaggtgc ctgtgcactt tgacggttag
      661 atgccaccat gtagggatta tcgcgagtgg ttgaccttac acttactcct taaatagcag
      721 tgagtaatgc atttgagctg tcccaggctc tgtctcctca gctcatttcc tactcttttt
      781 ctctatataa ctcattctat taaatacatt gcaccaaaga gatatggaga cataaacctg
      841 taatgaatga ggctgggctt ttctgtaata agcttccttt tataatactg gtcagcttag
      901 ctctctcaga tcctatcctg tggaatttag ttattatgtg tatttatgta gtatttcaaa
      961 catttcaaaa tgctttcatc tatgtttatc acattttaat accacagcac ttataatgat
     1021 gtcactacat atagaagctc aaagttaagg gatttgctga agactgtaaa gttaatggaa
     1081 gaattgagac aaaaatccag tgtagctggc cacttatcca gggctttttc tacttcatca
     1141 caaggaatgt tttgaaagtg tctgcttttt ttatccttaa aattcacctg tcagggaggc
     1201 attaaaaatt tggaaatgta tgccagcaaa atgtgagctc tgtatttttt ggcattctta
     1261 tgtttgggtt taataagatt aagaaaatga tactgggaat tttctttttc ctgaaacttt
     1321 gaatcaccct agtaagtcaa agtactaaaa aatgtactag atcattaaga cttatgtgct
     1381 cttactgatt gaaagatttt ttatgttttc cttgtaataa aggacctaaa ccgaaggtac
     1441 ctgagaagac tgtgctatgg tattattttt tttctctgac ttttaaattc ttgtttagtt
     1501 tataaacatg catgcacctt aataacctca taaactctgg tcaaagacta atgcctatca
     1561 gatccatgac cacaacacag aagatttgtc tcatttactc cagagagaat gattcctctc
     1621 aaagacaatt ctcacagctc ttccttctcc cttagaatat ttagaagcaa attagggagc
     1681 tgtcaggtct ctgagcccaa gcctgcacgt atacatccag atggcctgaa gcaactgaag
     1741 aaccacaaaa gaagtgaaaa taaccaattc ctgccttaac tgatgacatt ccaccactgt
     1801 gatttgttcc tgccccacct taactgatca attagccttg tgacattcct tctcctggac
     1861 aattagactc aggagctccc caccagagca ccttgtgacc cccgcccctg cccgctagag
     1921 aataacccac tttgaccgta atttcccact accaacccaa atcctataaa actgccccac
     1981 cccatctccc tttgctgact ccttttttgg actcagtctg cctgcaccca ggtgattaaa
     2041 aagctttatt gctcacacaa a
//
