LOCUS       NM_002933                914 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens ribonuclease A family member 1, pancreatic (RNASE1),
            transcript variant 4, mRNA.
ACCESSION   NM_002933
VERSION     NM_002933.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 914)
  AUTHORS   Lee,H.H., Wang,Y.N., Yang,W.H., Xia,W., Wei,Y., Chan,L.C.,
            Wang,Y.H., Jiang,Z., Xu,S., Yao,J., Qiu,Y., Hsu,Y.H., Hwang,W.L.,
            Yan,M., Cha,J.H., Hsu,J.L., Shen,J., Ye,Y., Wu,X., Hou,M.F.,
            Tseng,L.M., Wang,S.C., Pan,M.R., Yang,C.H., Wang,Y.L.,
            Yamaguchi,H., Pang,D., Hortobagyi,G.N., Yu,D. and Hung,M.C.
  TITLE     Human ribonuclease 1 serves as a secretory ligand of ephrin A4
            receptor and induces breast tumor initiation
  JOURNAL   Nat Commun 12 (1), 2788 (2021)
   PUBMED   33986289
  REMARK    GeneRIF: Human ribonuclease 1 serves as a secretory ligand of
            ephrin A4 receptor and induces breast tumor initiation.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 914)
  AUTHORS   Nechooshtan,G., Yunusov,D., Chang,K. and Gingeras,T.R.
  TITLE     Processing by RNase 1 forms tRNA halves and distinct Y RNA
            fragments in the extracellular environment
  JOURNAL   Nucleic Acids Res 48 (14), 8035-8049 (2020)
   PUBMED   32609822
  REMARK    GeneRIF: Processing by RNase 1 forms tRNA halves and distinct Y RNA
            fragments in the extracellular environment.
REFERENCE   4  (bases 1 to 914)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 914)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 914)
  AUTHORS   Sakakibara,R., Hashida,K., Kitahara,T. and Ishiguro,M.
  TITLE     Characterization of a unique nonsecretory ribonuclease from urine
            of pregnant women
  JOURNAL   J Biochem 111 (3), 325-330 (1992)
   PUBMED   1587793
REFERENCE   7  (bases 1 to 914)
  AUTHORS   Haugg,M. and Schein,C.H.
  TITLE     The DNA sequences of the human and hamster secretory ribonucleases
            determined with the polymerase chain reaction (PCR)
  JOURNAL   Nucleic Acids Res 20 (3), 612 (1992)
   PUBMED   1741299
REFERENCE   8  (bases 1 to 914)
  AUTHORS   Sakakibara,R., Hashida,K., Tominaga,N., Sakai,K., Ishiguro,M.,
            Imamura,S., Ohmatsu,F. and Sato,E.
  TITLE     A putative mouse oocyte maturation inhibitory protein from urine of
            pregnant women: N-terminal sequence homology with human
            nonsecretory ribonuclease
  JOURNAL   Chem Pharm Bull (Tokyo) 39 (1), 146-149 (1991)
   PUBMED   2049798
REFERENCE   9  (bases 1 to 914)
  AUTHORS   Mizuta,K., Awazu,S., Yasuda,T. and Kishi,K.
  TITLE     Purification and characterization of three ribonucleases from human
            kidney: comparison with urine ribonucleases
  JOURNAL   Arch Biochem Biophys 281 (1), 144-151 (1990)
   PUBMED   2383019
REFERENCE   10 (bases 1 to 914)
  AUTHORS   Beintema,J.J., Blank,A., Schieven,G.L., Dekker,C.A., Sorrentino,S.
            and Libonati,M.
  TITLE     Differences in glycosylation pattern of human secretory
            ribonucleases
  JOURNAL   Biochem J 255 (2), 501-505 (1988)
   PUBMED   3202829
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC005324.1, BP385178.1 and
            AL133371.3.
            
            On Nov 23, 2018 this sequence version replaced NM_002933.4.
            
            Summary: This gene encodes a member of the pancreatic-type of
            secretory ribonucleases, a subset of the ribonuclease A
            superfamily. The encoded endonuclease cleaves internal
            phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It
            prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic
            nucleotides, with a pH optimum near 8.0. The encoded protein is
            monomeric and more commonly acts to degrade ds-RNA over ss-RNA.
            Alternative splicing occurs at this locus and four transcript
            variants encoding the same protein have been identified. [provided
            by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (4) is the predominant transcript.
            Variants 1, 2, 3 and 4 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BM769726.1, SRR5189667.40331.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000397967.5/ ENSP00000381057.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-781               BC005324.1         34-814
            782-786             BP385178.1         577-581
            787-914             AL133371.3         53280-53407         c
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q11.2"
     gene            1..914
                     /gene="RNASE1"
                     /gene_synonym="RAC1; RIB1; RNS1"
                     /note="ribonuclease A family member 1, pancreatic"
                     /db_xref="GeneID:6035"
                     /db_xref="HGNC:HGNC:10044"
                     /db_xref="MIM:180440"
     exon            1..48
                     /gene="RNASE1"
                     /gene_synonym="RAC1; RIB1; RNS1"
                     /inference="alignment:Splign:2.1.0"
     exon            49..914
                     /gene="RNASE1"
                     /gene_synonym="RAC1; RIB1; RNS1"
                     /inference="alignment:Splign:2.1.0"
     CDS             74..544
                     /gene="RNASE1"
                     /gene_synonym="RAC1; RIB1; RNS1"
                     /EC_number="4.6.1.18"
                     /note="RNase upI-1; RIB-1; RNase 1; RNase A; HP-RNase;
                     ribonuclease 1; ribonuclease A C1; ribonuclease, RNase A
                     family, 1 (pancreatic)"
                     /codon_start=1
                     /product="ribonuclease pancreatic precursor"
                     /protein_id="NP_002924.1"
                     /db_xref="CCDS:CCDS9559.1"
                     /db_xref="GeneID:6035"
                     /db_xref="HGNC:HGNC:10044"
                     /db_xref="MIM:180440"
                     /translation="MALEKSLVRLLLLVLILLVLGWVQPSLGKESRAKKFQRQHMDSD
                     SSPSSSSTYCNQMMRRRNMTQGRCKPVNTFVHEPLVDVQNVCFQEKVTCKNGQGNCYK
                     SNSSMHITDCRLTNGSRYPNCAYRTSPKERHIIVACEGSPYVPVHFDASVEDST"
     regulatory      761..766
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RNASE1"
                     /gene_synonym="RAC1; RIB1; RNS1"
                     /note="hexamer: AATAAA"
     polyA_site      786
                     /gene="RNASE1"
                     /gene_synonym="RAC1; RIB1; RNS1"
                     /note="major polyA site"
     regulatory      888..893
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RNASE1"
                     /gene_synonym="RAC1; RIB1; RNS1"
                     /note="hexamer: ATTAAA"
     polyA_site      914
                     /gene="RNASE1"
                     /gene_synonym="RAC1; RIB1; RNS1"
ORIGIN      
        1 gcagaaactg gccttccatc tctctcagac accaagctgc agatccaggc ttttctggga
       61 aagtgaggcc accatggctc tggagaagtc tcttgtccgg ctccttctgc ttgtcctgat
      121 actgctggtg ctgggctggg tccagccttc cctgggcaag gaatcccggg ccaagaaatt
      181 ccagcggcag catatggact cagacagttc ccccagcagc agctccacct actgtaacca
      241 aatgatgagg cgccggaata tgacacaggg gcggtgcaaa ccagtgaaca cctttgtgca
      301 cgagcccctg gtagatgtcc agaatgtctg tttccaggaa aaggtcacct gcaagaacgg
      361 gcagggcaac tgctacaaga gcaactccag catgcacatc acagactgcc gcctgacaaa
      421 cggctccagg taccccaact gtgcataccg gaccagcccg aaggagagac acatcattgt
      481 ggcctgtgaa gggagcccat atgtgccagt ccactttgat gcttctgtgg aggactctac
      541 ctaaggtcag agcagcgaga taccccacct ccctcaacct catcctctcc acagctgcct
      601 cttccctctt ccttccctgc tgtgaaagaa gtaactacag ttagggctcc tattcaacac
      661 acacatgctt ccctttcctg agtcccatcc ctgcgtgatt ttgggggtga agagtgggtt
      721 gtgaggtggg ccccatgtta acccctccac tctttctttc aataaaacgc agttgcaaac
      781 acctgatttc tgaagcggtt ctgtctaggt actgtttctg gcattgcctt ccagcaaggg
      841 gtaagaactg taaatctgat tcactttgga gaacggtgaa tggagtaatt aaatgccttc
      901 ccttctgact tgga
//
