LOCUS       NM_006911               1009 bp    mRNA    linear   PRI 17-APR-2022
DEFINITION  Homo sapiens relaxin 1 (RLN1), mRNA.
ACCESSION   NM_006911
VERSION     NM_006911.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Luck K, Kim DK, Lambourne L, Spirohn K, Begg BE, Bian W, Brignall
            R, Cafarelli T, Campos-Laborie FJ, Charloteaux B, Choi D, Cote AG,
            Daley M, Deimling S, Desbuleux A, Dricot A, Gebbia M, Hardy MF,
            Kishore N, Knapp JJ, Kovacs IA, Lemmens I, Mee MW, Mellor JC,
            Pollis C, Pons C, Richardson AD, Schlabach S, Teeking B, Yadav A,
            Babor M, Balcha D, Basha O, Bowman-Colin C, Chin SF, Choi SG,
            Colabella C, Coppin G, D'Amata C, De Ridder D, De Rouck S,
            Duran-Frigola M, Ennajdaoui H, Goebels F, Goehring L, Gopal A,
            Haddad G, Hatchi E, Helmy M, Jacob Y, Kassa Y, Landini S, Li R, van
            Lieshout N, MacWilliams A, Markey D, Paulson JN, Rangarajan S,
            Rasla J, Rayhan A, Rolland T, San-Miguel A, Shen Y, Sheykhkarimli
            D, Sheynkman GM, Simonovsky E, Tasan M, Tejeda A, Tropepe V,
            Twizere JC, Wang Y, Weatheritt RJ, Weile J, Xia Y, Yang X,
            Yeger-Lotem E, Zhong Q, Aloy P, Bader GD, De Las Rivas J, Gaudet S,
            Hao T, Rak J, Tavernier J, Hill DE, Vidal M, Roth FP and Calderwood
            MA.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Guo X, Liu Y, Huang X, Wang Y, Qu J and Lv Y.
  TITLE     Serum relaxin as a diagnostic and prognostic marker in patients
            with epithelial ovarian cancer
  JOURNAL   Cancer Biomark 21 (1), 81-87 (2017)
   PUBMED   29060928
  REMARK    GeneRIF: Results indicate that serum relaxin may be a clinically
            useful indicator for diagnostic and prognostic evaluation in
            epithelial ovarian cancer (EOC) patients.
REFERENCE   3  (bases 1 to 1009)
  AUTHORS   Garella R, Squecco R and Baccari MC.
  TITLE     Site-related Effects of Relaxin in the Gastrointestinal Tract
            Through Nitric Oxide Signalling: An Updated Report
  JOURNAL   Curr Protein Pept Sci 18 (12), 1254-1262 (2017)
   PUBMED   28606038
  REMARK    GeneRIF: The digestive tract has been shown to express relaxin
            receptors; the hormone appears to exert site-specific effects
            acting at the neural or at the smooth muscle level, mainly by a
            nitric oxide-mediated mechanism. (Review)
            Review article
REFERENCE   4  (bases 1 to 1009)
  AUTHORS   Winslow JW, Shih A, Bourell JH, Weiss G, Reed B, Stults JT and
            Goldsmith LT.
  TITLE     Human seminal relaxin is a product of the same gene as human luteal
            relaxin
  JOURNAL   Endocrinology 130 (5), 2660-2668 (1992)
   PUBMED   1572287
REFERENCE   5  (bases 1 to 1009)
  AUTHORS   Eigenbrot C, Randal M, Quan C, Burnier J, O'Connell L, Rinderknecht
            E and Kossiakoff AA.
  TITLE     X-ray structure of human relaxin at 1.5 A. Comparison to insulin
            and implications for receptor binding determinants
  JOURNAL   J Mol Biol 221 (1), 15-21 (1991)
   PUBMED   1656049
REFERENCE   6  (bases 1 to 1009)
  AUTHORS   Bullesbach EE and Schwabe C.
  TITLE     Total synthesis of human relaxin and human relaxin derivatives by
            solid-phase peptide synthesis and site-directed chain combination
  JOURNAL   J Biol Chem 266 (17), 10754-10761 (1991)
   PUBMED   2040595
REFERENCE   7  (bases 1 to 1009)
  AUTHORS   Hansell DJ, Bryant-Greenwood GD and Greenwood FC.
  TITLE     Expression of the human relaxin H1 gene in the decidua,
            trophoblast, and prostate
  JOURNAL   J Clin Endocrinol Metab 72 (4), 899-904 (1991)
   PUBMED   2005217
REFERENCE   8  (bases 1 to 1009)
  AUTHORS   Stults JT, Bourell JH, Canova-Davis E, Ling VT, Laramee GR, Winslow
            JW, Griffin PR, Rinderknecht E and Vandlen RL.
  TITLE     Structural characterization by mass spectrometry of native and
            recombinant human relaxin
  JOURNAL   Biomed Environ Mass Spectrom 19 (11), 655-664 (1990)
   PUBMED   2076464
REFERENCE   9  (bases 1 to 1009)
  AUTHORS   Crawford,R.J., Hudson,P., Shine,J., Niall,H.D., Eddy,R.L. and
            Shows,T.B.
  TITLE     Two human relaxin genes are on chromosome 9
  JOURNAL   EMBO J 3 (10), 2341-2345 (1984)
   PUBMED   6548703
REFERENCE   10 (bases 1 to 1009)
  AUTHORS   Hudson,P., John,M., Crawford,R., Haralambidis,J., Scanlon,D.,
            Gorman,J., Tregear,G., Shine,J. and Niall,H.
  TITLE     Relaxin gene expression in human ovaries and the predicted
            structure of a human preprorelaxin by analysis of cDNA clones
  JOURNAL   EMBO J 3 (10), 2333-2339 (1984)
   PUBMED   6548702
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL135786.17, BC005956.1,
            AI363233.1 and AV742436.1.
            
            On Nov 23, 2018 this sequence version replaced NM_006911.3.
            
            Summary: Relaxins are known endocrine and autocrine/paracrine
            hormones, belonging to the insulin gene superfamily. In humans
            there are three non-allelic relaxin genes, RLN1, RLN2 and RLN3,
            where RLN1 and RLN2 share high sequence homology. The protein
            encoded by this gene is synthesized as a single-chain polypeptide
            but the active form consists of an A chain and a B chain linked by
            disulfide bonds. Relaxin is produced by the ovary, and targets the
            mammalian reproductive system to ripen the cervix, elongate the
            pubic symphysis and inhibit uterine contraction. It may have
            additional roles in enhancing sperm motility, regulating blood
            pressure, controlling heart rate and releasing oxytocin and
            vasopressin. [provided by RefSeq, Jan 2013].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC005956.1, X00949.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145240, SAMEA2153427
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000223862.2/ ENSP00000223862.1
            RefSeq Select criteria :: based on expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 AL135786.17        41954-41956         c
            4-967               BC005956.1         1-964
            968-977             AI363233.1         11-20               c
            978-1007            AV742436.1         372-401
            1008-1009           AL135786.17        37010-37011         c
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9p24.1"
     gene            1..1009
                     /gene="RLN1"
                     /gene_synonym="bA12D24.3.1; bA12D24.3.2; H1; H1RLX; RLXH1"
                     /note="relaxin 1"
                     /db_xref="GeneID:6013"
                     /db_xref="HGNC:HGNC:10026"
                     /db_xref="MIM:179730"
     exon            1..341
                     /gene="RLN1"
                     /gene_synonym="bA12D24.3.1; bA12D24.3.2; H1; H1RLX; RLXH1"
                     /inference="alignment:Splign:2.1.0"
     CDS             131..688
                     /gene="RLN1"
                     /gene_synonym="bA12D24.3.1; bA12D24.3.2; H1; H1RLX; RLXH1"
                     /note="prorelaxin H1; preprorelaxin H1"
                     /codon_start=1
                     /product="prorelaxin H1 preproprotein"
                     /protein_id="NP_008842.1"
                     /db_xref="CCDS:CCDS6462.1"
                     /db_xref="GeneID:6013"
                     /db_xref="HGNC:HGNC:10026"
                     /db_xref="MIM:179730"
                     /translation="MPRLFLFHLLEFCLLLNQFSRAVAAKWKDDVIKLCGRELVRAQI
                     AICGMSTWSKRSLSQEDAPQTPRPVAEIVPSFINKDTETIIIMLEFIANLPPELKAAL
                     SERQPSLPELQQYVPALKDSNLSFEEFKKLIRNRQSEAADSNPSELKYLGLDTHSQKK
                     RRPYVALFEKCCLIGCTKRSLAKYC"
     exon            342..1009
                     /gene="RLN1"
                     /gene_synonym="bA12D24.3.1; bA12D24.3.2; H1; H1RLX; RLXH1"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      882
                     /gene="RLN1"
                     /gene_synonym="bA12D24.3.1; bA12D24.3.2; H1; H1RLX; RLXH1"
                     /note="major polyA site"
     regulatory      951..956
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RLN1"
                     /gene_synonym="bA12D24.3.1; bA12D24.3.2; H1; H1RLX; RLXH1"
                     /note="hexamer: AATAAA"
     polyA_site      977
                     /gene="RLN1"
                     /gene_synonym="bA12D24.3.1; bA12D24.3.2; H1; H1RLX; RLXH1"
                     /note="major polyA site"
     regulatory      987..992
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RLN1"
                     /gene_synonym="bA12D24.3.1; bA12D24.3.2; H1; H1RLX; RLXH1"
                     /note="hexamer: AATACA"
     polyA_site      1009
                     /gene="RLN1"
                     /gene_synonym="bA12D24.3.1; bA12D24.3.2; H1; H1RLX; RLXH1"
ORIGIN      
        1 actggttgag ccgggtaggg aaagcagcct aaagcccggg acaggcacac aggcccaggt
       61 gtgtaggcca cagcagccgc agtcctgaaa ggctgcaaca cccagacctc caggagagac
      121 caggcccagg atgcctcgcc tgttcttgtt ccacctgcta gaattctgtt tactactgaa
      181 ccaattttcc agagcagtcg cggccaaatg gaaggacgat gttattaaat tatgcggccg
      241 cgaattagtt cgcgcgcaga ttgccatttg cggcatgagc acctggagca aaaggtctct
      301 gagccaggaa gatgctcctc agacacctag accagtggca gaaattgtac catccttcat
      361 caacaaagat acagaaacta taattatcat gttggaattc attgctaatt tgccaccgga
      421 gctgaaggca gccctatctg agaggcaacc atcattacca gagctacagc agtatgtacc
      481 tgcattaaag gattccaatc ttagctttga agaatttaag aaacttattc gcaataggca
      541 aagtgaagcc gcagacagca atccttcaga attaaaatac ttaggcttgg atactcattc
      601 tcaaaaaaag agacgaccct acgtggcact gtttgagaaa tgttgcctaa ttggttgtac
      661 caaaaggtct cttgctaaat attgctgaga tgaagctaat tgtgcacatc ttgtctaatt
      721 ttcacacata gtcttgatga catttcactg atgcttctgt caggtcccac taattattag
      781 aatataagaa atctttatta atgtttagat ttttcatttg gtgtgtaaga aaatattctt
      841 tgtatgattg tagtttctgt aaatgacact ttctatgctg aagtcttttt gtctttttat
      901 taacagtata attgtgttga ttctttttaa tgctgttaac ttaaaattac aataaaacct
      961 ttgccacttt aaccatacat aataacaata cagtacctaa caacagtta
//
