LOCUS       NM_018343               3909 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens RIO kinase 2 (RIOK2), transcript variant 1, mRNA.
ACCESSION   NM_018343
VERSION     NM_018343.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Matsuzaki,Y., Naito,Y., Miura,N., Mori,T., Watabe,Y., Yoshimoto,S.,
            Shibahara,T., Takano,M. and Honda,K.
  TITLE     RIOK2 Contributes to Cell Growth and Protein Synthesis in Human
            Oral Squamous Cell Carcinoma
  JOURNAL   Curr Oncol 30 (1), 381-391 (2022)
   PUBMED   36661680
  REMARK    GeneRIF: RIOK2 Contributes to Cell Growth and Protein Synthesis in
            Human Oral Squamous Cell Carcinoma.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Ghosh,S., Raundhal,M., Myers,S.A., Carr,S.A., Chen,X., Petsko,G.A.
            and Glimcher,L.H.
  TITLE     Identification of RIOK2 as a master regulator of human blood cell
            development
  JOURNAL   Nat Immunol 23 (1), 109-121 (2022)
   PUBMED   34937919
  REMARK    GeneRIF: Identification of RIOK2 as a master regulator of human
            blood cell development.
REFERENCE   3  (bases 1 to 3909)
  AUTHORS   Cerezo,E.L., Houles,T., Lie,O., Sarthou,M.K., Audoynaud,C.,
            Lavoie,G., Halladjian,M., Cantaloube,S., Froment,C.,
            Burlet-Schiltz,O., Henry,Y., Roux,P.P., Henras,A.K. and Romeo,Y.
  TITLE     RIOK2 phosphorylation by RSK promotes synthesis of the human small
            ribosomal subunit
  JOURNAL   PLoS Genet 17 (6), e1009583 (2021)
   PUBMED   34125833
  REMARK    GeneRIF: RIOK2 phosphorylation by RSK promotes synthesis of the
            human small ribosomal subunit.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3909)
  AUTHORS   Gao,S., Sha,Z., Zhou,J., Wu,Y., Song,Y., Li,C., Liu,X., Zhang,T.
            and Yu,R.
  TITLE     BYSL contributes to tumor growth by cooperating with the mTORC2
            complex in gliomas
  JOURNAL   Cancer Biol Med 18 (1), 88-104 (2021)
   PUBMED   33628587
  REMARK    GeneRIF: BYSL contributes to tumor growth by cooperating with the
            mTORC2 complex in gliomas.
REFERENCE   5  (bases 1 to 3909)
  AUTHORS   Song,Y., Li,C., Jin,L., Xing,J., Sha,Z., Zhang,T., Ji,D., Yu,R. and
            Gao,S.
  TITLE     RIOK2 is negatively regulated by miR-4744 and promotes glioma cell
            migration/invasion through epithelial-mesenchymal transition
  JOURNAL   J Cell Mol Med 24 (8), 4494-4509 (2020)
   PUBMED   32125767
  REMARK    GeneRIF: RIOK2 is negatively regulated by miR-4744 and promotes
            glioma cell migration/invasion through epithelial-mesenchymal
            transition.
REFERENCE   6  (bases 1 to 3909)
  AUTHORS   Read,R.D., Fenton,T.R., Gomez,G.G., Wykosky,J., Vandenberg,S.R.,
            Babic,I., Iwanami,A., Yang,H., Cavenee,W.K., Mischel,P.S.,
            Furnari,F.B. and Thomas,J.B.
  TITLE     A kinome-wide RNAi screen in Drosophila Glia reveals that the RIO
            kinases mediate cell proliferation and survival through TORC2-Akt
            signaling in glioblastoma
  JOURNAL   PLoS Genet 9 (2), e1003253 (2013)
   PUBMED   23459592
  REMARK    GeneRIF: reduced expression of RIOK1 or RIOK2 disrupted Akt
            signaling and caused cell cycle exit, apoptosis, and
            chemosensitivity in glioblastoma cells
REFERENCE   7  (bases 1 to 3909)
  AUTHORS   Liu,T., Deng,M., Li,J., Tong,X., Wei,Q. and Ye,X.
  TITLE     Phosphorylation of right open reading frame 2 (Rio2) protein kinase
            by polo-like kinase 1 regulates mitotic progression
  JOURNAL   J Biol Chem 286 (42), 36352-36360 (2011)
   PUBMED   21880710
  REMARK    GeneRIF: Plk1-mediated phosphorylation of Rio2 regulates
            metaphase-anaphase transition during mitotic progression.
REFERENCE   8  (bases 1 to 3909)
  AUTHORS   Johnson,M.P., Roten,L.T., Dyer,T.D., East,C.E., Forsmo,S.,
            Blangero,J., Brennecke,S.P., Austgulen,R. and Moses,E.K.
  TITLE     The ERAP2 gene is associated with preeclampsia in Australian and
            Norwegian populations
  JOURNAL   Hum Genet 126 (5), 655-666 (2009)
   PUBMED   19578876
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   9  (bases 1 to 3909)
  AUTHORS   Zemp,I., Wild,T., O'Donohue,M.F., Wandrey,F., Widmann,B.,
            Gleizes,P.E. and Kutay,U.
  TITLE     Distinct cytoplasmic maturation steps of 40S ribosomal subunit
            precursors require hRio2
  JOURNAL   J Cell Biol 185 (7), 1167-1180 (2009)
   PUBMED   19564402
  REMARK    GeneRIF: Results show that the protein kinase human Rio2 (hRio2) is
            part of a late 40S preribosomal particle in human cells.
REFERENCE   10 (bases 1 to 3909)
  AUTHORS   LaRonde-LeBlanc,N. and Wlodawer,A.
  TITLE     A family portrait of the RIO kinases
  JOURNAL   J Biol Chem 280 (45), 37297-37300 (2005)
   PUBMED   16183636
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DB240299.1, AK002021.1, AC008865.3 and CA415109.1.
            
            On Nov 23, 2018 this sequence version replaced NM_018343.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.70800.1,
                                           SRR1803611.277357.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000283109.8/ ENSP00000283109.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-7                 DB240299.1         55-61
            8-502               AK002021.1         1-495
            503-509             AC008865.3         191076-191082       c
            510-1100            AK002021.1         503-1093
            1101-1101           AC008865.3         181734-181734       c
            1102-1696           AK002021.1         1095-1689
            1697-3908           AC008865.3         174783-176994       c
            3909-3909           CA415109.1         18-18               c
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q15"
     gene            1..3909
                     /gene="RIOK2"
                     /gene_synonym="RIO2"
                     /note="RIO kinase 2"
                     /db_xref="GeneID:55781"
                     /db_xref="HGNC:HGNC:18999"
                     /db_xref="MIM:617754"
     exon            1..122
                     /gene="RIOK2"
                     /gene_synonym="RIO2"
                     /inference="alignment:Splign:2.1.0"
     CDS             57..1715
                     /gene="RIOK2"
                     /gene_synonym="RIO2"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     serine/threonine-protein kinase RIO2"
                     /codon_start=1
                     /product="serine/threonine-protein kinase RIO2 isoform 1"
                     /protein_id="NP_060813.2"
                     /db_xref="CCDS:CCDS4089.1"
                     /db_xref="GeneID:55781"
                     /db_xref="HGNC:HGNC:18999"
                     /db_xref="MIM:617754"
                     /translation="MGKVNVAKLRYMSRDDFRVLTAVEMGMKNHEIVPGSLIASIASL
                     KHGGCNKVLRELVKHKLIAWERTKTVQGYRLTNAGYDYLALKTLSSRQVVESVGNQMG
                     VGKESDIYIVANEEGQQFALKLHRLGRTSFRNLKNKRDYHKHRHNVSWLYLSRLSAMK
                     EFAYMKALYERKFPVPKPIDYNRHAVVMELINGYPLCQIHHVEDPASVYDEAMELIVK
                     LANHGLIHGDFNEFNLILDESDHITMIDFPQMVSTSHPNAEWYFDRDVKCIKDFFMKR
                     FSYESELFPTFKDIRREDTLDVEVSASGYTKEMQADDELLHPLGPDDKNIETKEGSEF
                     SFSDGEVAEKAEVYGSENESERNCLEESEGCYCRSSGDPEQIKEDSLSEESADARSFE
                     MTEFNQALEEIKGQVVENNSVTEFSEEKNRTENYNRQDGQRVQGGVPAGSDEYEDECP
                     HLIALSSLNREFRPFRDEENVGAMNQYRTRTLSITSSGSAVSCSTIPPELVKQKVKRQ
                     LTKQQKSAVRRRLQKGEANIFTKQRRENMQNIKSSLEAASFWGE"
     exon            123..261
                     /gene="RIOK2"
                     /gene_synonym="RIO2"
                     /inference="alignment:Splign:2.1.0"
     exon            262..378
                     /gene="RIOK2"
                     /gene_synonym="RIO2"
                     /inference="alignment:Splign:2.1.0"
     exon            379..554
                     /gene="RIOK2"
                     /gene_synonym="RIO2"
                     /inference="alignment:Splign:2.1.0"
     exon            555..643
                     /gene="RIOK2"
                     /gene_synonym="RIO2"
                     /inference="alignment:Splign:2.1.0"
     exon            644..835
                     /gene="RIOK2"
                     /gene_synonym="RIO2"
                     /inference="alignment:Splign:2.1.0"
     exon            836..928
                     /gene="RIOK2"
                     /gene_synonym="RIO2"
                     /inference="alignment:Splign:2.1.0"
     exon            929..1453
                     /gene="RIOK2"
                     /gene_synonym="RIO2"
                     /inference="alignment:Splign:2.1.0"
     exon            1454..1550
                     /gene="RIOK2"
                     /gene_synonym="RIO2"
                     /inference="alignment:Splign:2.1.0"
     exon            1551..3909
                     /gene="RIOK2"
                     /gene_synonym="RIO2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1817..1822
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RIOK2"
                     /gene_synonym="RIO2"
                     /note="hexamer: AATAAA"
     regulatory      1821..1826
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RIOK2"
                     /gene_synonym="RIO2"
                     /note="hexamer: AATAAA"
     polyA_site      1840
                     /gene="RIOK2"
                     /gene_synonym="RIO2"
     polyA_site      1844
                     /gene="RIOK2"
                     /gene_synonym="RIO2"
                     /note="major polyA site"
     regulatory      3888..3893
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RIOK2"
                     /gene_synonym="RIO2"
                     /note="hexamer: AATAAA"
     polyA_site      3909
                     /gene="RIOK2"
                     /gene_synonym="RIO2"
ORIGIN      
        1 gccttacgct ttgcggctgt cgtcggagag gcatctgggt tcggactggg gccgccatgg
       61 ggaaagtgaa tgtggccaag ttgcgttaca tgagccgaga tgacttcagg gtcttgaccg
      121 cggttgaaat gggcatgaag aaccatgaaa ttgttcccgg cagtttgatt gcttctatag
      181 ccagccttaa acatggtggc tgtaataaag ttttaagaga attagtgaaa cataaactca
      241 tagcttggga gcgtaccaaa actgtccagg gctatcggtt gacaaatgca ggatatgatt
      301 acctagcttt gaaaacactt tcttctaggc aagtagttga gtctgttgga aaccagatgg
      361 gtgttggcaa agaatcagat atttacattg ttgcaaatga agaaggacaa caatttgcat
      421 taaagcttca cagactagga agaacctcgt ttcgaaattt gaaaaacaaa cgcgattatc
      481 ataaacatag gcacaatgtg tcatggctat atttatctcg tctctctgcc atgaaggaat
      541 ttgcctatat gaaggcattg tatgagagga aatttccagt tccaaagcca attgattaca
      601 atcgtcatgc agtggtcatg gaactcataa atggttatcc actatgtcag atacaccatg
      661 ttgaagatcc tgcatcagta tatgatgaag ctatggaact aattgtcaaa cttgcaaatc
      721 atgggctgat tcatggagat tttaatgaat ttaatctcat tttggatgaa agtgaccata
      781 tcaccatgat tgattttcca cagatggttt caacttctca tcccaatgct gagtggtatt
      841 ttgacagaga tgttaaatgc attaaagatt tctttatgaa acgtttcagc tacgaaagtg
      901 agctttttcc aacttttaag gatatcagga gagaagacac tcttgatgtg gaggtttctg
      961 ccagtggcta cacaaaggaa atgcaggcag atgatgaact gcttcatcca ttaggtccag
     1021 atgataaaaa tattgaaaca aaagagggat ctgaattctc attttcagat ggagaagtgg
     1081 cagaaaaagc agaggtttac gggtcagaaa atgaaagtga acggaactgt ctagaagaat
     1141 cagagggctg ctattgcaga tcatctggag accctgaaca aataaaggaa gacagtttat
     1201 cagaagagag tgctgatgca cggagttttg aaatgactga attcaatcaa gctttagaag
     1261 aaataaaagg gcaggttgtt gaaaacaact ctgtaactga attttctgag gagaaaaaca
     1321 gaactgaaaa ttacaacagg caagatggtc agagagttca aggaggagtc cctgctggct
     1381 ctgacgagta tgaagatgaa tgccctcatc taattgcctt gtcgtcatta aatagagaat
     1441 tcaggccttt cagagatgaa gaaaatgtgg gagctatgaa tcagtataga acaagaactc
     1501 tgagtatcac ttcttcaggc agtgctgtaa gctgttcaac aattcctcca gaactggtga
     1561 aacagaaggt gaaacgtcag ttgacaaaac agcaaaaatc agctgtcaga cgtcgattgc
     1621 agaaaggaga agcaaatata tttaccaagc aacgtaggga aaacatgcaa aatatcaaat
     1681 caagtttgga agcagccagc ttttggggag aataatatat ttaggatctt ggatatgttt
     1741 aatatatttt ttaaagttac tgtaattcct ttttgagccc tcatttgtct tttttgagcc
     1801 aaggctatca tatattaata aataaaccct ctttcatcta taaatttggt cattcagtat
     1861 gtgtcttgca aagaataatt acctgccaaa gcagtccatt tttttcaaag taatttgggt
     1921 aactacagtt acgtaacttt attagagaca tttgcatatt tttaaaaatc taaataacat
     1981 ttttgttgtt ttctgtttgt tgaggatgat atgctacaat gcacataaag caattagaac
     2041 aatgtttagc acattcttaa gcattcaata aaacatggct attagaatca taatgattct
     2101 gaaattctgc tgacaatttg gttgcatttt atttggcaac tggaggactt cactgtctta
     2161 tttttcttta gaagtaaaga taagcctttg gaagtaaaga taagccagtg atgcacaatt
     2221 tgcttaatta attgattaat aatctgtttt gtgccactta cattggtaat gtggtcatga
     2281 gaatggaaac ttacttgaga tagtctacca caatttttac agttggcagc ttgtaagcag
     2341 gcctgagtag cagaagagtt aggggttggg gaaggtggag tagaatgagg aatgaaatgt
     2401 tctttctcat atgcttatta tataagtggg tccggaacat gttctcacct caaggttaac
     2461 aaatacctaa tgcaggaaag aaaatacata gccatggtcc cttcctcaag gactttataa
     2521 ttcagtgaga tgtaaagatg agtgcttaag tgtatgcacc tgattattgt ctcttcattg
     2581 ccgcataagt cggaaaaggg gatcacttgc agcgaaaaat ggccactaga tggcaatatc
     2641 accccatagt caccaaacag gattacttgc atccctaatt atgttctacc agagggaata
     2701 tagtacagac ttttccaaga ttgataaaga cagcagattc cctttcacct ttgccaaatt
     2761 aactctacta gctatttgca agacctgtaa ttatctaata aattggctta aatatataga
     2821 agctagaaaa tgccaattcc tcttccctca aaagttacct gacattcaaa tatttaaata
     2881 cccttgcttt gcattttaga tatatctttg aattcaggta ttcattttgt ataatctctc
     2941 aacttttaag catttttata atgtaaagtt gaaaacagct taatagaacc acagaatgtt
     3001 tattttggag tcattttctt atattcagag ctatgaaagc aaattgggaa taaagcctta
     3061 tcctgctcag ctttacctag ttgactcagt tgacttttga gtatctacta cataaaaagc
     3121 ccattactat taagaaactc aagacaaaca tgatttttac ctttaatagc ctaagatttc
     3181 ttgtgggcca aggaattaat tattccaatc agaaatggag gccttacctc atttgggcaa
     3241 agattagtgc tagttattga agatttacta ataaatgatc tgttaaggaa tttagttttt
     3301 tttggatatg ttgttttggt tgttgaaaac taaggtataa ttataatagt tattttttga
     3361 agatatgttt ggctagccat caattagaag ttagagagtt tgtcctaaca gataagctat
     3421 aaccctcaca ttttaaaaca tgcatagtga acaatgaaca aaaacaaatt caaaatttta
     3481 ctaatgtaaa gctgtatatt gtgattgtta aaacactttt ttttgcctag gagttccaga
     3541 tttaaatcct ggttctacac ttcccaaacc tcaggtacct tctgtggcct ctctaacctc
     3601 aattttttta cttgtaaaat ggggacattt aatagtatac ctcagtgtcg tgaagactaa
     3661 atgaagtacc acttgtaaag agtttattac agtgcctgac atatactgta tggttagtac
     3721 tgctaaaatg acttcttaag gttaccaaaa ggaaaattcc acaagctgac cacctttcct
     3781 attgtgatgg tcactggtat ttcagctatg atcctttgtt gtgtatttat ccagactgaa
     3841 agatgttttt ctagtaacca tcttgcatcg tcttagtctc cccattaaat aaacttttcc
     3901 tacaaacta
//
