LOCUS       NM_001348484            7711 bp    mRNA    linear   PRI 13-FEB-2025
DEFINITION  Homo sapiens regulating synaptic membrane exocytosis 2 (RIMS2),
            transcript variant 5, mRNA.
ACCESSION   NM_001348484 XM_017014013
VERSION     NM_001348484.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7711)
  AUTHORS   Mechaussier,S., Almoallem,B., Zeitz,C., Van Schil,K., Jeddawi,L.,
            Van Dorpe,J., Duenas Rey,A., Condroyer,C., Pelle,O., Polak,M.,
            Boddaert,N., Bahi-Buisson,N., Cavallin,M., Bacquet,J.L.,
            Mouallem-Beziere,A., Zambrowski,O., Sahel,J.A., Audo,I., Kaplan,J.,
            Rozet,J.M., De Baere,E. and Perrault,I.
  TITLE     Loss of Function of RIMS2 Causes a Syndromic Congenital Cone-Rod
            Synaptic Disease with Neurodevelopmental and Pancreatic Involvement
  JOURNAL   Am J Hum Genet 106 (6), 859-871 (2020)
   PUBMED   32470375
  REMARK    GeneRIF: Loss of Function of RIMS2 Causes a Syndromic Congenital
            Cone-Rod Synaptic Disease with Neurodevelopmental and Pancreatic
            Involvement.
            Erratum:[Am J Hum Genet. 2020 Sep 3;107(3):580. doi:
            10.1016/j.ajhg.2020.08.004.. PMID: 32888510]
REFERENCE   2  (bases 1 to 7711)
  AUTHORS   Hirano,M., Takada,Y., Wong,C.F., Yamaguchi,K., Kotani,H.,
            Kurokawa,T., Mori,M.X., Snutch,T.P., Ronjat,M., De Waard,M. and
            Mori,Y.
  TITLE     C-terminal splice variants of P/Q-type Ca2+ channel CaV2.1 alpha1
            subunits are differentially regulated by Rab3-interacting molecule
            proteins
  JOURNAL   J Biol Chem 292 (22), 9365-9381 (2017)
   PUBMED   28377503
  REMARK    GeneRIF: Electrophysiological characterization of VDCC currents
            revealed that the suppressive effect of RIM2alpha on
            voltage-dependent inactivation (VDI) was stronger than that of
            RIM1alpha for the CaV2.1 variant containing the region encoded by
            exons 44 and 47.
REFERENCE   3  (bases 1 to 7711)
  AUTHORS   Gelernter,J., Sherva,R., Koesterer,R., Almasy,L., Zhao,H.,
            Kranzler,H.R. and Farrer,L.
  TITLE     Genome-wide association study of cocaine dependence and related
            traits: FAM53B identified as a risk gene
  JOURNAL   Mol Psychiatry 19 (6), 717-723 (2014)
   PUBMED   23958962
REFERENCE   4  (bases 1 to 7711)
  AUTHORS   Yoo,J.C., Lim,Ty., Park,J.S., Hah,Y.S., Park,N., Hong,S.G.,
            Park,J.Y. and Yoon,T.J.
  TITLE     SYT14L, especially its C2 domain, is involved in regulating
            melanocyte differentiation
  JOURNAL   J Dermatol Sci 72 (3), 246-251 (2013)
   PUBMED   23999003
REFERENCE   5  (bases 1 to 7711)
  AUTHORS   Kim,K.T., Lee,J.S., Lee,B.W., Seok,H., Jeon,H.S., Kim,J.H. and
            Chung,J.H.
  TITLE     Association between regulating synaptic membrane exocytosis 2 gene
            polymorphisms and degenerative lumbar scoliosis
  JOURNAL   Biomed Rep 1 (4), 619-623 (2013)
   PUBMED   24648997
REFERENCE   6  (bases 1 to 7711)
  AUTHORS   Sun,L., Bittner,M.A. and Holz,R.W.
  TITLE     Rim, a component of the presynaptic active zone and modulator of
            exocytosis, binds 14-3-3 through its N terminus
  JOURNAL   J Biol Chem 278 (40), 38301-38309 (2003)
   PUBMED   12871946
REFERENCE   7  (bases 1 to 7711)
  AUTHORS   Fukuda,M.
  TITLE     Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and
            Noc2. Identification of a critical determinant of Rab3A/Rab27A
            recognition by Rim2
  JOURNAL   J Biol Chem 278 (17), 15373-15380 (2003)
   PUBMED   12578829
REFERENCE   8  (bases 1 to 7711)
  AUTHORS   Wang,Y. and Sudhof,T.C.
  TITLE     Genomic definition of RIM proteins: evolutionary amplification of a
            family of synaptic regulatory proteins
  JOURNAL   Genomics 81 (2), 126-137 (2003)
   PUBMED   12620390
REFERENCE   9  (bases 1 to 7711)
  AUTHORS   Kashima,Y., Miki,T., Shibasaki,T., Ozaki,N., Miyazaki,M., Yano,H.
            and Seino,S.
  TITLE     Critical role of cAMP-GEFII--Rim2 complex in incretin-potentiated
            insulin secretion
  JOURNAL   J Biol Chem 276 (49), 46046-46053 (2001)
   PUBMED   11598134
REFERENCE   10 (bases 1 to 7711)
  AUTHORS   Fenster,S.D., Chung,W.J., Zhai,R., Cases-Langhoff,C., Voss,B.,
            Garner,A.M., Kaempf,U., Kindler,S., Gundelfinger,E.D. and
            Garner,C.C.
  TITLE     Piccolo, a presynaptic zinc finger protein structurally related to
            bassoon
  JOURNAL   Neuron 25 (1), 203-214 (2000)
   PUBMED   10707984
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC012213.8, AC007751.3,
            AC090686.4, AP001572.3, AC107933.6 and AP002849.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 9, 2021 this sequence version replaced NM_001348484.2.
            
            Summary: The protein encoded by this gene is a presynaptic protein
            that interacts with RAB3, a protein important for normal
            neurotransmitter release. The encoded protein can also bind several
            other synaptic proteins, including UNC-13 homolog B,
            ELKS/Rab6-interacting/CAST family member 1, and synaptotagmin 1.
            This protein is involved in synaptic membrane exocytosis.
            Polymorphisms in this gene have been associated with degenerative
            lumbar scoliosis. [provided by RefSeq, Feb 2017].
            
            Transcript Variant: This variant (5) encodes the longest isoform
            (e).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA2157437,
                              SAMN03267754 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000696799.1/ ENSP00000512879.1
            RefSeq Select criteria :: based on manual assertion, conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-453               AC012213.8         17731-18183         c
            454-486             AC007751.3         41452-41484         c
            487-573             AC007751.3         40979-41065         c
            574-784             AC090686.4         46217-46427
            785-796             AC090686.4         65440-65451
            797-1107            AC090686.4         115358-115668
            1108-2033           AP001572.3         45731-46656
            2034-2242           AP001572.3         70754-70962
            2243-2362           AP001572.3         72486-72605
            2363-2462           AP001572.3         75928-76027
            2463-2586           AP001572.3         76847-76970
            2587-2633           AP001572.3         78874-78920
            2634-2746           AP001572.3         82105-82217
            2747-2877           AP001572.3         91696-91826
            2878-3049           AP001572.3         96984-97155
            3050-3203           AP001572.3         103206-103359
            3204-3272           AP001572.3         121498-121566
            3273-3429           AP001572.3         135783-135939
            3430-3546           AC107933.6         200571-200687       c
            3547-3726           AC107933.6         176371-176550       c
            3727-3836           AC107933.6         175377-175486       c
            3837-3869           AC107933.6         148634-148666       c
            3870-3970           AC107933.6         121380-121480       c
            3971-4143           AC107933.6         96349-96521         c
            4144-4385           AC107933.6         41144-41385         c
            4386-4527           AP002849.2         141841-141982
            4528-4640           AP002849.2         145626-145738
            4641-4742           AP002849.2         146412-146513
            4743-4882           AP002849.2         147949-148088
            4883-7711           AP002849.2         148527-151355
FEATURES             Location/Qualifiers
     source          1..7711
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q22.3"
     gene            1..7711
                     /gene="RIMS2"
                     /gene_synonym="CRSDS; OBOE; RAB3IP3; RIM2"
                     /note="regulating synaptic membrane exocytosis 2"
                     /db_xref="GeneID:9699"
                     /db_xref="HGNC:HGNC:17283"
                     /db_xref="MIM:606630"
     exon            1..453
                     /gene="RIMS2"
                     /gene_synonym="CRSDS; OBOE; RAB3IP3; RIM2"
                     /inference="alignment:Splign:2.1.0"
     CDS             278..5101
                     /gene="RIMS2"
                     /gene_synonym="CRSDS; OBOE; RAB3IP3; RIM2"
                     /note="isoform e is encoded by transcript variant 5; RAB3
                     interacting protein 3; regulating synaptic membrane
                     exocytosis protein 2; nuclear protein; non-small cell lung
                     cancer RimL3a protein; non-small cell lung cancer RimL3c
                     protein; rab3-interacting molecule 2; rab-3-interacting
                     protein 3; rab-3-interacting molecule 2; Rab3-interacting
                     protein"
                     /codon_start=1
                     /product="regulating synaptic membrane exocytosis protein
                     2 isoform e"
                     /protein_id="NP_001335413.1"
                     /db_xref="CCDS:CCDS94327.1"
                     /db_xref="GeneID:9699"
                     /db_xref="HGNC:HGNC:17283"
                     /db_xref="MIM:606630"
                     /translation="MSAPVGPRGRLAPIPAASQPPLQPEMPDLSHLTEEERKIILAVM
                     DRQKKEEEKEQSVLKVKEEHKPQLTQWFPFSGITELVNNVLQPQQKQQNEKEPQTKLH
                     QQFEMYKEQVKKMGEESQQQQEQKGDAPTCGICHKTKFADGCGHNCSYCQTKFCARCG
                     GRVSLRSNKEDKVVMWVCNLCRKQQEILTKSGAWFYNSGSNTPQQPDQKVLRGLRNEE
                     APQEKKPKLHEQTQFQGPSGDLSVPAVEKSRSHGLTRQHSIKNGSGVKHHIASDIASD
                     RKRSPSVSRDQNRRYDQREEREEYSQYATSDTAMPRSPSDYADRRSQHEPQFYEDSDH
                     LSYRDSNRRSHRHSKEYIVDDEDVESRDEYERQRREEEYQSRYRSDPNLARYPVKPQP
                     YEEQMRIHAEVSRARHERRHSDVSLANADLEDSRISMLRMDRPSRQRSISERRAAMEN
                     QRSYSMERTREAQGPSSYAQRTTNHSPPTPRRSPLPIDRPDLRRTDSLRKQHHLDPSS
                     AVRKTKREKMETMLRNDSLSSDQSESVRPPPPKPHKSKKGGKMRQISLSSSEEELAST
                     PEYTSCDDVEIESESVSEKGDSQKGKRKTSEQAVLSDSNTRSERQKEMMYFGGHSLEE
                     DLEWSEPQIKDSGVDTCSSTTLNEEHSHSDKHPVTWQPSKDGDRLIGRILLNKRLKDG
                     SVPRDSGAMLGLKVVGGKMTESGRLCAFITKVKKGSLADTVGHLRPGDEVLEWNGRLL
                     QGATFEEVYNIILESKPEPQVELVVSRPIGDIPRIPDSTHAQLESSSSSFESQKMDRP
                     SISVTSPMSPGMLRDVPQFLSGQLSIKLWFDKVGHQLIVTILGAKDLPSREDGRPRNP
                     YVKIYFLPDRSDKNKRRTKTVKKTLEPKWNQTFIYSPVHRREFRERMLEITLWDQARV
                     REEESEFLGEILIELETALLDDEPHWYKLQTHDVSSLPLPHPSPYMPRRQLHGESPTR
                     RLQRSKRISDSEVSDYDCDDGIGVVSDYRHDGRDLQSSTLSVPEQVMSSNHCSPSGSP
                     HRVDVIGRTRSWSPSVPPPQSRNVEQGLRGTRTMTGHYNTISRMDRHRVMDDHYSPDR
                     DRDCEAADRQPYHRSRSTEQRPLLERTTTRSRSTERPDTNLMRSMPSLMTGRSAPPSP
                     ALSRSHPRTGSVQTSPSSTPVAGRRGRQLPQLPPKGTLDRKELDSTRRRYAGTMDIEE
                     RNRQMKINKYKQVAGSDPRLEQDYHSKYRSGWDPHRGADNVSTKSSDSDVSDISAVSR
                     TSSASRFSSTSYMSVQSERPGGNKKISVFTSKMQSRQMGISGKNMTKSTSISGDMCSL
                     EKNDGSQSDTAVGTLGTSGKKRRSSLGAKMVAIVGLSRKSRSASQLSQTEAGGKKLRS
                     TVQRSTETGLAVEMRNWMTRQASRESTDGSMNSYSSEGNLIFPGVRLASDSQFSDFLD
                     GLGPAQLVGRQTLATPAMGDIQVGMMDKKGQLEVEIIRARGLVVKPGSKTLPAPYVKV
                     YLLDNGVCIAKKKTKVARKTLEPLYQQLLSFEESPQGKVLQIIVWGDYGRMDHKSFMG
                     VAQILLDELELSNMVIGWFKLFPPSSLVDPTLAPLTRRASQSSLESSTGPSYSRS"
     exon            454..486
                     /gene="RIMS2"
                     /gene_synonym="CRSDS; OBOE; RAB3IP3; RIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            487..573
                     /gene="RIMS2"
                     /gene_synonym="CRSDS; OBOE; RAB3IP3; RIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            574..784
                     /gene="RIMS2"
                     /gene_synonym="CRSDS; OBOE; RAB3IP3; RIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            785..796
                     /gene="RIMS2"
                     /gene_synonym="CRSDS; OBOE; RAB3IP3; RIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            797..1107
                     /gene="RIMS2"
                     /gene_synonym="CRSDS; OBOE; RAB3IP3; RIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            1108..2033
                     /gene="RIMS2"
                     /gene_synonym="CRSDS; OBOE; RAB3IP3; RIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            2034..2242
                     /gene="RIMS2"
                     /gene_synonym="CRSDS; OBOE; RAB3IP3; RIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            2243..2362
                     /gene="RIMS2"
                     /gene_synonym="CRSDS; OBOE; RAB3IP3; RIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            2363..2462
                     /gene="RIMS2"
                     /gene_synonym="CRSDS; OBOE; RAB3IP3; RIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            2463..2586
                     /gene="RIMS2"
                     /gene_synonym="CRSDS; OBOE; RAB3IP3; RIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            2587..2633
                     /gene="RIMS2"
                     /gene_synonym="CRSDS; OBOE; RAB3IP3; RIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            2634..2746
                     /gene="RIMS2"
                     /gene_synonym="CRSDS; OBOE; RAB3IP3; RIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            2747..2877
                     /gene="RIMS2"
                     /gene_synonym="CRSDS; OBOE; RAB3IP3; RIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            2878..3049
                     /gene="RIMS2"
                     /gene_synonym="CRSDS; OBOE; RAB3IP3; RIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            3050..3203
                     /gene="RIMS2"
                     /gene_synonym="CRSDS; OBOE; RAB3IP3; RIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            3204..3272
                     /gene="RIMS2"
                     /gene_synonym="CRSDS; OBOE; RAB3IP3; RIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            3273..3429
                     /gene="RIMS2"
                     /gene_synonym="CRSDS; OBOE; RAB3IP3; RIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            3430..3546
                     /gene="RIMS2"
                     /gene_synonym="CRSDS; OBOE; RAB3IP3; RIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            3547..3726
                     /gene="RIMS2"
                     /gene_synonym="CRSDS; OBOE; RAB3IP3; RIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            3727..3836
                     /gene="RIMS2"
                     /gene_synonym="CRSDS; OBOE; RAB3IP3; RIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            3837..3869
                     /gene="RIMS2"
                     /gene_synonym="CRSDS; OBOE; RAB3IP3; RIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            3870..3970
                     /gene="RIMS2"
                     /gene_synonym="CRSDS; OBOE; RAB3IP3; RIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            3971..4143
                     /gene="RIMS2"
                     /gene_synonym="CRSDS; OBOE; RAB3IP3; RIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            4144..4385
                     /gene="RIMS2"
                     /gene_synonym="CRSDS; OBOE; RAB3IP3; RIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            4386..4527
                     /gene="RIMS2"
                     /gene_synonym="CRSDS; OBOE; RAB3IP3; RIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            4528..4640
                     /gene="RIMS2"
                     /gene_synonym="CRSDS; OBOE; RAB3IP3; RIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            4641..4742
                     /gene="RIMS2"
                     /gene_synonym="CRSDS; OBOE; RAB3IP3; RIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            4743..4882
                     /gene="RIMS2"
                     /gene_synonym="CRSDS; OBOE; RAB3IP3; RIM2"
                     /inference="alignment:Splign:2.1.0"
     exon            4883..7711
                     /gene="RIMS2"
                     /gene_synonym="CRSDS; OBOE; RAB3IP3; RIM2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7689..7694
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RIMS2"
                     /gene_synonym="CRSDS; OBOE; RAB3IP3; RIM2"
                     /note="hexamer: AATAAA"
     polyA_site      7711
                     /gene="RIMS2"
                     /gene_synonym="CRSDS; OBOE; RAB3IP3; RIM2"
                     /note="major polyA site"
ORIGIN      
        1 agttcccctt tcccttgaac cgctcacttc acagcccttc gcccccggga agaagaaaca
       61 tttcccgaag cgcactcctc agccctcctt ccccacgcgc tcgccctccc ctccccctgc
      121 ttttcttggg ggaggggggc tgtcgccttg gattgaaggc cattgatttg tatgtatttg
      181 tcccagcgct ggaggctgcc ccagccgccg cgccggtgcc gccgctgcca gtggagttgc
      241 ctccccgctt ccctagggtg gttcggctcc accaaacatg tcggctcctg tcgggccccg
      301 gggccgcctg gctcccatcc cggcggcctc tcagccgcct ctgcagcccg agatgcctga
      361 cctcagccac ctcacggagg aggagaggaa aatcatcctg gccgtcatgg ataggcagaa
      421 gaaagaagag gagaaggagc agtccgtgct caaggtcaaa gaagaacaca aaccacaact
      481 gacacagtgg tttcccttta gtggaatcac tgaactggta aataacgttc ttcagcccca
      541 gcaaaaacaa caaaatgaaa aggagcccca gacaaaactg catcagcagt ttgaaatgta
      601 taaagagcag gtaaagaaga tgggagaaga atcacagcaa cagcaagaac agaagggtga
      661 tgcgccaacc tgtggtatct gccacaaaac aaagtttgct gatggatgtg gccataactg
      721 ttcatattgc caaacaaagt tctgtgctcg ttgtggaggt cgagtgtcat tacgctcaaa
      781 caaggaggac aaagtggtta tgtgggtatg taatttgtgc cgaaaacaac aagaaatcct
      841 cactaaatca ggagcatggt tttataatag tggatctaat acaccacagc aacctgatca
      901 aaaggttctt cgagggctaa gaaatgagga ggcacctcag gagaagaaac caaaactaca
      961 tgagcagacc cagttccaag gaccctcagg tgacttatct gtacctgcag tggagaaaag
     1021 tcgatctcat gggctcacaa gacagcattc tattaaaaat gggtcaggcg tgaagcatca
     1081 cattgccagt gacatagctt cagacaggaa aagaagccca tctgtgtcca gagatcagaa
     1141 tagaagatac gaccaaaggg aagaaagaga ggaatattca cagtatgcta cttcggatac
     1201 cgcaatgcct agatctccat cagattatgc tgataggcga tctcaacatg aacctcagtt
     1261 ttatgaagac tctgatcatt taagttatag ggactccaac aggagaagtc ataggcattc
     1321 caaagaatat attgtagatg atgaggatgt ggaaagcaga gatgaatacg aaaggcaaag
     1381 gagagaggaa gagtaccagt cacgctaccg aagtgatccg aatttggccc gttatccagt
     1441 aaagccacaa ccctatgaag aacaaatgcg gatccatgct gaagtgtccc gagcacggca
     1501 tgagagaagg catagtgatg tttctttggc aaatgctgat ctggaagatt ccaggatttc
     1561 tatgctaagg atggatcgac catcaaggca aagatctata tcagaacgta gagctgccat
     1621 ggaaaatcag cgatcttatt caatggaaag aactcgagag gctcagggac caagttctta
     1681 tgcacaaagg accacaaacc atagtcctcc tacccccagg aggagtccac tacccataga
     1741 tagaccagac ttgaggcgta ctgactcact acggaaacag caccacttag atcctagctc
     1801 tgctgtaaga aaaacaaaac gggaaaaaat ggaaacaatg ttaaggaatg attctctcag
     1861 ttcagaccag tcagagtcag tgagacctcc accaccaaag cctcataaat caaagaaagg
     1921 cggtaaaatg cgccagattt cgttgagcag ttcagaggag gaattggctt ccacgcctga
     1981 atatacaagt tgtgatgatg ttgagattga aagtgagagt gtaagtgaaa aaggggacag
     2041 tcaaaaggga aaaagaaaaa ctagtgagca ggcagttttg tcggactcta acaccaggtc
     2101 tgagagacaa aaggaaatga tgtactttgg tggccactct ttggaagagg atttggaatg
     2161 gtctgaacct cagattaagg actctggggt agatacgtgt agtagcacaa cccttaacga
     2221 ggagcatagc catagtgata agcaccctgt aacctggcaa ccatctaaag atggagatcg
     2281 tttaattggt cgcattttat taaataagcg tctaaaagat ggaagtgtac ctcgagattc
     2341 aggagcaatg cttggcttga aggttgtagg aggaaagatg actgaatcag gtcggctttg
     2401 tgcatttatt actaaagtaa aaaaaggaag tttagctgat actgtaggac atcttagacc
     2461 aggtgatgaa gtattagaat ggaatggaag actactgcaa ggagccacat ttgaggaagt
     2521 gtacaacatc attctagaat ccaaacctga accacaagta gaacttgtag tttcaaggcc
     2581 tattggagat ataccgcgaa tacctgatag cacacatgca caactggagt ccagttctag
     2641 ctcctttgaa tctcaaaaaa tggatcgtcc ttctatttct gttacctctc ccatgagtcc
     2701 tggaatgttg agggatgtcc cacagttctt atcaggacaa ctttcaataa aactatggtt
     2761 tgacaaggtt ggtcaccaat taatagttac aattttggga gcaaaagatc tcccttccag
     2821 ggaagatggg aggccaagga atccttatgt taaaatttac tttcttccag acagaagtga
     2881 taaaaacaag agaagaacta aaacagtaaa gaaaacattg gaacccaaat ggaaccaaac
     2941 attcatttat tctccagtcc accgaagaga atttcgggaa cgaatgctag agattaccct
     3001 ttgggatcaa gctcgtgttc gagaggaaga aagtgaattc ttaggcgaga ttttaattga
     3061 attagaaaca gcattattag atgatgagcc acattggtac aaacttcaga cgcatgatgt
     3121 ctcttcattg ccacttcccc acccttctcc atatatgcca cgaagacagc tccatggaga
     3181 gagcccaaca cggaggttgc aaaggtcaaa gagaataagt gatagtgaag tctctgacta
     3241 tgactgtgat gatggaattg gtgtagtatc agattatcga catgatggtc gagatcttca
     3301 aagctcaaca ttatcagtgc cagaacaagt aatgtcatca aaccactgtt caccatcagg
     3361 gtctcctcat cgagtagatg ttataggaag gactagatca tggtcaccca gtgtccctcc
     3421 tccacaaagt cggaatgtgg aacaggggct tcgagggacc cgcactatga ccggacatta
     3481 taatacaatt agccgaatgg acagacatcg tgtcatggat gaccattatt ctccagatag
     3541 agacagggat tgtgaagcag cagatagaca gccatatcac agatccagat caacagaaca
     3601 acggcctctc cttgagcgga ccaccacccg ctccagatcc actgaacgtc ctgatacaaa
     3661 cctcatgagg tcgatgcctt cattaatgac tggaagatct gcccctcctt cacctgcctt
     3721 atcgaggtct catcctcgta ctgggtctgt ccagacaagc ccatcaagta ctccagtcgc
     3781 aggacgaagg ggccgacagc ttccacagct tccaccaaag ggaacgttgg atagaaaaga
     3841 actggactct acaaggagaa gatatgcagg tactatggat atagaggaga gaaatcgcca
     3901 aatgaaaatt aacaaataca aacaggtagc cggatcagat cccagactgg aacaagatta
     3961 ccattcgaag tatcgatcag gatgggatcc tcatagaggg gcagataatg tttctactaa
     4021 atcttcggac agtgatgtaa gtgatatatc tgcggtttca aggactagta gtgcttctcg
     4081 tttcagcagc acaagctaca tgtctgtcca atcagaacgc ccaggaggaa acaagaaaat
     4141 cagtgtcttt acatccaaaa tgcaaagcag acaaatgggc atatcaggga agaacatgac
     4201 aaaaagcacc agcatcagtg gagacatgtg ctcactggag aagaatgatg gcagccagtc
     4261 tgacactgca gtgggcacct tgggcaccag tggcaaaaag cggcgctcta gccttggtgc
     4321 caaaatggta gctatcgttg gtctgtcacg gaaaagtcgc agtgcttctc agctcagcca
     4381 aacggaagca ggaggtaaaa aactaaggag cactgtccaa agaagtacag aaacaggcct
     4441 ggccgtggaa atgaggaact ggatgactcg acaggcaagc cgagagtcta cagatggtag
     4501 catgaacagc tacagctcag aaggaaatct gattttccct ggtgttcgct tggcctctga
     4561 tagccagttc agtgatttcc tggatggcct tggccctgct cagctagtgg gacgccagac
     4621 tctggcaaca cctgcaatgg gtgacattca ggtaggaatg atggacaaaa agggacagct
     4681 ggaggtagaa atcatccggg cccgtggcct tgttgtaaaa ccaggttcca agacactgcc
     4741 agcaccgtat gtaaaagtgt atctattaga taacggagtc tgcatagcca aaaagaaaac
     4801 aaaagtggca agaaaaacgc tggaacccct ttaccagcag ctattatctt tcgaagagag
     4861 tccacaagga aaagttttac agatcatcgt ctggggagat tatggccgca tggatcacaa
     4921 atcttttatg ggagtggccc agatactttt agatgaacta gagctatcca atatggtgat
     4981 cggatggttc aaacttttcc caccttcctc cctagtagat ccaaccttgg cccctctgac
     5041 aagaagagct tcccaatcat ctctggaaag ttcaactgga ccttcttact ctcgttcata
     5101 gcagctgtaa aaaaattgtt gtcacagcaa ccagcgttac aaaaaaaaaa aaaaaaatca
     5161 caggttgcaa accctggtaa cactgcatgc ttaatgttgt gtcttctgag cctgtttcta
     5221 gggatacaaa gcaatcctgt gttctcagag gaagttgcac acattgtgcc ctaaagaagg
     5281 ccctcaggtg aaagagcaga gctgtgaaga actatcagat ttggaattca atgacactcg
     5341 agttctggtc caatctgaag ccatggatta atctcaaaga atcagtcagt ttcatgcaac
     5401 agaagccctt ttcaatggca cctttatatt tttatcattc ctttttcttc atttatctaa
     5461 ccccaaagcc ctgatatgcc acagaaatgg agctatacag ccatgaagcg gtgttacagg
     5521 tgaggagtgt aatcctagga agcatcaggt gaaaagcagg agaccaaaga agtggtcagg
     5581 aacaatcatc agccctcctc tgggcgggaa tcagagcagt cagtccagca ggaagagtgg
     5641 cagactttgt agctccatgg gcacgtcaat tactaatgct aagatgtgtt ggactctgaa
     5701 aaacaaaatt ctgtggctac actgtactga atgaaattaa agaaactttt tttgcatgga
     5761 cacagattag ctgaatactt aaattatttt cttggggctg caacttgcaa aaaaaaaaaa
     5821 aagaataaaa atcagccatt ttcaacaatt tatattattt ttaaaaataa atttcactag
     5881 tgcatggttt taaaaaggag agagaatgca acagggtgat acaaagatac accatgttta
     5941 ttctttaatc atagtctgtg ttttggcaga cattacaaat ggaaatactt tctagaagat
     6001 acttaaaatt ctctttatgt gacaaataag tataatatat tcaatttatt tccatgttaa
     6061 atatacaaat cttatgaagt tcaatatgtg caaatttttc acatctttct ccttctctca
     6121 ctttacctct tctccctctt ttaaactttt ctttctccct gccagagtga accttatact
     6181 aaaaaattac aagttttgat ctgatcctct ctcatacccc atgtttgatt cagagctgta
     6241 gatgcctctg aatttgcgaa tttctcaagg gaaaattaac tttaagagct ttctttattt
     6301 caagcatgtt gaaaaggatt ttgcaacatg acttgggagt acattaaagt aagtcagcat
     6361 gtatttgacg aagaagatat ttgaactttt gcagtttatt gtacagtgca tggtaatttt
     6421 ttcaccttta aaattcagtt tacaggaaaa ttctaaaatc atgttgccat tgtgatgtcc
     6481 aataaatttg tttttagcac cagcattatt catacagggg ttaaagtatt atttgtagaa
     6541 ggtcttaggt tttgtttgtt ttttaatcat ttaaagcaat ttctttagcc agtttccatt
     6601 tactatgtga atagaagcac tgctaaaaat tgggaaccct gaaacacagg gctgtttatt
     6661 aattcatttt tctgtagtaa aattcaattt ttcacaaatt atatttctaa agaaatatag
     6721 taaacataaa tttgcaacaa ttttaaagct ccagttttta ggtgactcaa agaaagtcat
     6781 tatgcctatt aatagttatt tgatgccatc accaaaagtc tatgtgaaaa tctcctaaag
     6841 tcaaaacccc tgcctttggt tttacagacg gttattacca ttgggtggag ctgcaaggtc
     6901 aaatttctcc taagttcccc tatttagagg aaaagtcact ggttattgta ataaaccacc
     6961 catggttctt tatgtacatt ttgataacac attattatag cttgatttta attttttgca
     7021 ttaatttttg aaatccacat acatctcatt tgtttaaatt aaggccatgc acaaatattt
     7081 tttttagttc agtgctgacc attaaaaact atcatgcttg atacggtgca aaagttaaaa
     7141 tgagtatcac taaaaatgcc ttctttttat gtggtgcaat atgaaataca ccaagactgt
     7201 gtcttgacat tctgatggac ccaggtaaag ttgttaaaag aacgaataaa actttattaa
     7261 aataatttag acacctgtgt accagcaaca attgatttaa tagacctata gtgtctatac
     7321 tatcccttag aataaaggtt tatgattttc ctgatactaa gatgcagtca cataatcttt
     7381 tgtgcatatt cctatacaaa ttatttctaa ttttaataag aaggacgtga ctacggaata
     7441 tttgtacata cttgtcatta tgcagtattt atttaaaagt tggtgttttt ttttaatttt
     7501 cacatctgca cctcgacttg tggtttagtc atgtaactag cactatgcca gtgaccgttg
     7561 ttgccctgta catagtatgt ttgaaaagta aagggaattc cagttgggaa aaaagggcag
     7621 attagtcctg taatgaacac caactaatgt aaatcaaatt cattctggtg atggtattta
     7681 acactttaaa taaaacattt tctttacagg c
//
