LOCUS       NM_014989               7984 bp    mRNA    linear   PRI 03-MAY-2024
DEFINITION  Homo sapiens regulating synaptic membrane exocytosis 1 (RIMS1),
            transcript variant 1, mRNA.
ACCESSION   NM_014989
VERSION     NM_014989.7
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7984)
  AUTHORS   Hsu,Y.H., Pintacuda,G., Liu,R., Nacu,E., Kim,A., Tsafou,K.,
            Petrossian,N., Crotty,W., Suh,J.M., Riseman,J., Martin,J.M.,
            Biagini,J.C., Mena,D., Ching,J.K.T., Malolepsza,E., Li,T.,
            Singh,T., Ge,T., Egri,S.B., Tanenbaum,B., Stanclift,C.R.,
            Apffel,A.M., Carr,S.A., Schenone,M., Jaffe,J., Fornelos,N.,
            Huang,H., Eggan,K.C. and Lage,K.
  CONSRTM   Schizophrenia Working Group of the Psychiatric Genomics Consortium;
            Stanley Global Asia Initiatives
  TITLE     Using brain cell-type-specific protein interactomes to interpret
            neurodevelopmental genetic signals in schizophrenia
  JOURNAL   iScience 26 (5), 106701 (2023)
   PUBMED   37207277
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7984)
  AUTHORS   Hirano,M., Takada,Y., Wong,C.F., Yamaguchi,K., Kotani,H.,
            Kurokawa,T., Mori,M.X., Snutch,T.P., Ronjat,M., De Waard,M. and
            Mori,Y.
  TITLE     C-terminal splice variants of P/Q-type Ca2+ channel CaV2.1 alpha1
            subunits are differentially regulated by Rab3-interacting molecule
            proteins
  JOURNAL   J Biol Chem 292 (22), 9365-9381 (2017)
   PUBMED   28377503
  REMARK    GeneRIF: Electrophysiological characterization of VDCC currents
            revealed that the suppressive effect of RIM2alpha on
            voltage-dependent inactivation (VDI) was stronger than that of
            RIM1alpha for the CaV2.1 variant containing the region encoded by
            exons 44 and 47.
REFERENCE   3  (bases 1 to 7984)
  AUTHORS   Warwick,A.N., Shawkat,F. and Lotery,A.J.
  TITLE     Retinitis pigmentosa and bilateral cystoid macular oedema in a
            patient heterozygous for the RIM1 mutation previously associated
            with cone-rod dystrophy 7
  JOURNAL   Ophthalmic Genet 38 (2), 178-182 (2017)
   PUBMED   27176872
  REMARK    GeneRIF: This is the first reported case of bilateral cystoid
            macular edema in association with the RIM1 mutation. Overall, our
            findings were more consistent with a phenotype of retinitis
            pigmentosa.
REFERENCE   4  (bases 1 to 7984)
  AUTHORS   Ruhle,F., Witten,A., Barysenka,A., Huge,A., Arning,A., Heller,C.,
            Krumpel,A., Mesters,R., Franke,A., Lieb,W., Riemenschneider,M.,
            Hiersche,M., Limperger,V., Nowak-Gottl,U. and Stoll,M.
  TITLE     Rare genetic variants in SMAP1, B3GAT2, and RIMS1 contribute to
            pediatric venous thromboembolism
  JOURNAL   Blood 129 (6), 783-790 (2017)
   PUBMED   28011674
  REMARK    GeneRIF: The study identified a region on chromosome 6 comprising
            the genes SMAP1, B3GAT2, and RIMS1 as novel susceptibility locus
            for pediatric venous thromboembolism.
REFERENCE   5  (bases 1 to 7984)
  CONSRTM   Schizophrenia Working Group of the Psychiatric Genomics Consortium
  TITLE     Biological insights from 108 schizophrenia-associated genetic loci
  JOURNAL   Nature 511 (7510), 421-427 (2014)
   PUBMED   25056061
REFERENCE   6  (bases 1 to 7984)
  AUTHORS   Wang,Y., Liu,X., Biederer,T. and Sudhof,T.C.
  TITLE     A family of RIM-binding proteins regulated by alternative splicing:
            Implications for the genesis of synaptic active zones
  JOURNAL   Proc Natl Acad Sci U S A 99 (22), 14464-14469 (2002)
   PUBMED   12391317
REFERENCE   7  (bases 1 to 7984)
  AUTHORS   Ohtsuka,T., Takao-Rikitsu,E., Inoue,E., Inoue,M., Takeuchi,M.,
            Matsubara,K., Deguchi-Tawarada,M., Satoh,K., Morimoto,K.,
            Nakanishi,H. and Takai,Y.
  TITLE     Cast: a novel protein of the cytomatrix at the active zone of
            synapses that forms a ternary complex with RIM1 and munc13-1
  JOURNAL   J Cell Biol 158 (3), 577-590 (2002)
   PUBMED   12163476
REFERENCE   8  (bases 1 to 7984)
  AUTHORS   Schoch,S., Castillo,P.E., Jo,T., Mukherjee,K., Geppert,M., Wang,Y.,
            Schmitz,F., Malenka,R.C. and Sudhof,T.C.
  TITLE     RIM1alpha forms a protein scaffold for regulating neurotransmitter
            release at the active zone
  JOURNAL   Nature 415 (6869), 321-326 (2002)
   PUBMED   11797009
REFERENCE   9  (bases 1 to 7984)
  AUTHORS   Coppola,T., Magnin-Luthi,S., Perret-Menoud,V., Gattesco,S.,
            Schiavo,G. and Regazzi,R.
  TITLE     Direct interaction of the Rab3 effector RIM with Ca2+ channels,
            SNAP-25, and synaptotagmin
  JOURNAL   J Biol Chem 276 (35), 32756-32762 (2001)
   PUBMED   11438518
REFERENCE   10 (bases 1 to 7984)
  AUTHORS   Betz,A., Thakur,P., Junge,H.J., Ashery,U., Rhee,J.S., Scheuss,V.,
            Rosenmund,C., Rettig,J. and Brose,N.
  TITLE     Functional interaction of the active zone proteins Munc13-1 and
            RIM1 in synaptic vesicle priming
  JOURNAL   Neuron 30 (1), 183-196 (2001)
   PUBMED   11343654
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL390056.7, AL034373.13,
            AL590011.5, AL445256.18 and AL035633.18.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Mar 31, 2021 this sequence version replaced NM_014989.6.
            
            Summary: The protein encoded by this gene is a RAS gene superfamily
            member that regulates synaptic vesicle exocytosis. This gene also
            plays a role in the regulation of voltage-gated calcium channels
            during neurotransmitter and insulin release. Mutations have
            suggested a role cognition and have been identified as the cause of
            cone-rod dystrophy type 7. Multiple transcript variants encoding
            different isoforms have been described for this gene. [provided by
            RefSeq, Mar 2012].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY190519.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN03465402, SAMN03465403
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000521978.6/ ENSP00000428417.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-638               AL390056.7         25491-26128
            639-719             AL034373.13        45634-45714
            720-933             AL590011.5         32952-33165
            934-945             AL590011.5         35978-35989
            946-1286            AL590011.5         115578-115918
            1287-2152           AL590011.5         118287-119152
            2153-2220           AL445256.18        14897-14964
            2221-2331           AL445256.18        16742-16852
            2332-2431           AL445256.18        18947-19046
            2432-2555           AL445256.18        23438-23561
            2556-2602           AL445256.18        26939-26985
            2603-2715           AL445256.18        29139-29251
            2716-2846           AL445256.18        31454-31584
            2847-3018           AL445256.18        32045-32216
            3019-3172           AL445256.18        32339-32492
            3173-3244           AL445256.18        33885-33956
            3245-3401           AL445256.18        39249-39405
            3402-3527           AL445256.18        40110-40235
            3528-3590           AL445256.18        41829-41891
            3591-3668           AL445256.18        46099-46176
            3669-3782           AL445256.18        46514-46627
            3783-3872           AL445256.18        47084-47173
            3873-3956           AL445256.18        55473-55556
            3957-4028           AL445256.18        65171-65242
            4029-4211           AL035633.18        5220-5402
            4212-4324           AL035633.18        6475-6587
            4325-4437           AL035633.18        21799-21911
            4438-4604           AL035633.18        28047-28213
            4605-4840           AL035633.18        48141-48376
            4841-4979           AL035633.18        105138-105276
            4980-5092           AL035633.18        107238-107350
            5093-5194           AL035633.18        112789-112890
            5195-5334           AL035633.18        113495-113634
            5335-7984           AL035633.18        115036-117685
FEATURES             Location/Qualifiers
     source          1..7984
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q13"
     gene            1..7984
                     /gene="RIMS1"
                     /gene_synonym="CORD7; RAB3IP2; RIM; RIM1"
                     /note="regulating synaptic membrane exocytosis 1"
                     /db_xref="GeneID:22999"
                     /db_xref="HGNC:HGNC:17282"
                     /db_xref="MIM:606629"
     exon            1..638
                     /gene="RIMS1"
                     /gene_synonym="CORD7; RAB3IP2; RIM; RIM1"
                     /inference="alignment:Splign:2.1.0"
     CDS             475..5553
                     /gene="RIMS1"
                     /gene_synonym="CORD7; RAB3IP2; RIM; RIM1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     RAB3-interacting protein 2; rab3-interacting molecule 1;
                     rab-3-interacting protein 2"
                     /codon_start=1
                     /product="regulating synaptic membrane exocytosis protein
                     1 isoform 1"
                     /protein_id="NP_055804.2"
                     /db_xref="CCDS:CCDS47449.1"
                     /db_xref="GeneID:22999"
                     /db_xref="HGNC:HGNC:17282"
                     /db_xref="MIM:606629"
                     /translation="MSSAVGPRGPRPPTVPPPMQELPDLSHLTEEERNIIMAVMDRQK
                     EEEEKEEAMLKCVVRDMAKPAACKTPRNAENQPHQPSPRLHQQFESYKEQVRKIGEEA
                     RRYQGEHKDDAPTCGICHKTKFADGCGHLCSYCRTKFCARCGGRVSLRSNNEDKVVMW
                     VCNLCRKQQEILTKSGAWFFGSGPQQTSQDGTLSDTATGAGSEVPREKKARLQERSRS
                     QTPLSTAAASSQDAAPPSAPPDRSKGAEPSQQALGPEQKQASSRSRSEPPRERKKTPG
                     LSEQNGKGALKSERKRVPKTSAQPVEGAVEERERKERRESRRLEKGRSQDYPDTPEKR
                     DEGKAADEEKQRKEEDYQTRYRSDPNLARYPVKPPPEEQQMRMHARVSRARHERRHSD
                     VALPRTEAGAALPEGKAGKRAPAAARASPPDSPRAYSAERTAETRAPGAKQLTNHSPP
                     APRHGPVPAEAPELKAQEPLRKQSRLDPSSAVLMRKAKREKVETMLRNDSLSSDQSES
                     VRPSPPKPHRSKRGGKKRQMSVSSSEEEGVSTPEYTSCEDVELESESVSEKGDLDYYW
                     LDPATWHSRETSPISSHPVTWQPSKEGDRLIGRVILNKRTTMPKDSGALLGLKVVGGK
                     MTDLGRLGAFITKVKKGSLADVVGHLRAGDEVLEWNGKPLPGATNEEVYNIILESKSE
                     PQVEIIVSRPIGDIPRIPESSHPPLESSSSSFESQKMERPSISVISPTSPGALKDAPQ
                     VLPGQLSVKLWYDKVGHQLIVNVLQATDLPARVDGRPRNPYVKMYFLPDRSDKSKRRT
                     KTVKKILEPKWNQTFVYSHVHRRDFRERMLEITVWDQPRVQEEESEFLGEILIELETA
                     LLDDEPHWYKLQTHDESSLPLPQPSPFMPRRHIHGESSSKKLQRSQRISDSDISDYEV
                     DDGIGVVPPVGYRSSARESKSTTLTVPEQQRTTHHRSRSVSPHRGNDQGKPRSRLPNV
                     PLQRSLDEIHPTRRSRSPTRHHDASRSPVDHRTRDVDSQYLSEQDSELLMLPRAKRGR
                     SAECLHTTRHLVRHYKTLPPKMPLLQSSSHWNIYSSILPAHTKTKSVTRQDISLHHEC
                     FNSTVLRFTDEILVSELQPFLDRARSASTNCLRPDTSLHSPERERGRWSPSLDRRRPP
                     SPRIQIQHASPENDRHSRKSERSSIQKQTRKGTASDAERVLPTCLSRRGHAAPRATDQ
                     PVIRGKHPARSRSSEHSSIRTLCSMHHLVPGGSAPPSPLLTRMHRQRSPTQSPPADTS
                     FSSRRGRQLPQVPVRSGSIEQASLVVEERTRQMKMKVHRFKQTTGSGSSQELDREQYS
                     KYNIHKDQYRSCDNVSAKSSDSDVSDVSAISRTSSASRLSSTSFMSEQSERPRGRISS
                     FTPKMQGRRMGTSGRSIMKSTSVSGEMYTLEHNDGSQSDTAVGTVGAGGKKRRSSLSA
                     KVVAIVSRRSRSTSQLSQTESGHKKLKSTIQRSTETGMAAEMRKMVRQPSRESTDGSI
                     NSYSSEGNLIFPGVRLGADSQFSDFLDGLGPAQLVGRQTLATPAMGDIQIGMEDKKGQ
                     LEVEVIRARSLTQKPGSKSTPAPYVKVYLLENGACIAKKKTRIARKTLDPLYQQSLVF
                     DESPQGKVLQVIVWGDYGRMDHKCFMGVAQILLEELDLSSMVIGWYKLFPPSSLVDPT
                     LTPLTRRASQSSLESSTGPPCIRS"
     exon            639..719
                     /gene="RIMS1"
                     /gene_synonym="CORD7; RAB3IP2; RIM; RIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            720..933
                     /gene="RIMS1"
                     /gene_synonym="CORD7; RAB3IP2; RIM; RIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            934..945
                     /gene="RIMS1"
                     /gene_synonym="CORD7; RAB3IP2; RIM; RIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            946..1286
                     /gene="RIMS1"
                     /gene_synonym="CORD7; RAB3IP2; RIM; RIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            1287..2152
                     /gene="RIMS1"
                     /gene_synonym="CORD7; RAB3IP2; RIM; RIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            2153..2220
                     /gene="RIMS1"
                     /gene_synonym="CORD7; RAB3IP2; RIM; RIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            2221..2331
                     /gene="RIMS1"
                     /gene_synonym="CORD7; RAB3IP2; RIM; RIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            2332..2431
                     /gene="RIMS1"
                     /gene_synonym="CORD7; RAB3IP2; RIM; RIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            2432..2555
                     /gene="RIMS1"
                     /gene_synonym="CORD7; RAB3IP2; RIM; RIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            2556..2602
                     /gene="RIMS1"
                     /gene_synonym="CORD7; RAB3IP2; RIM; RIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            2603..2715
                     /gene="RIMS1"
                     /gene_synonym="CORD7; RAB3IP2; RIM; RIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            2716..2846
                     /gene="RIMS1"
                     /gene_synonym="CORD7; RAB3IP2; RIM; RIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            2847..3018
                     /gene="RIMS1"
                     /gene_synonym="CORD7; RAB3IP2; RIM; RIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            3019..3172
                     /gene="RIMS1"
                     /gene_synonym="CORD7; RAB3IP2; RIM; RIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            3173..3244
                     /gene="RIMS1"
                     /gene_synonym="CORD7; RAB3IP2; RIM; RIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            3245..3401
                     /gene="RIMS1"
                     /gene_synonym="CORD7; RAB3IP2; RIM; RIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            3402..3527
                     /gene="RIMS1"
                     /gene_synonym="CORD7; RAB3IP2; RIM; RIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            3528..3590
                     /gene="RIMS1"
                     /gene_synonym="CORD7; RAB3IP2; RIM; RIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            3591..3668
                     /gene="RIMS1"
                     /gene_synonym="CORD7; RAB3IP2; RIM; RIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            3669..3782
                     /gene="RIMS1"
                     /gene_synonym="CORD7; RAB3IP2; RIM; RIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            3783..3872
                     /gene="RIMS1"
                     /gene_synonym="CORD7; RAB3IP2; RIM; RIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            3873..3956
                     /gene="RIMS1"
                     /gene_synonym="CORD7; RAB3IP2; RIM; RIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            3957..4028
                     /gene="RIMS1"
                     /gene_synonym="CORD7; RAB3IP2; RIM; RIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            4029..4211
                     /gene="RIMS1"
                     /gene_synonym="CORD7; RAB3IP2; RIM; RIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            4212..4324
                     /gene="RIMS1"
                     /gene_synonym="CORD7; RAB3IP2; RIM; RIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            4325..4437
                     /gene="RIMS1"
                     /gene_synonym="CORD7; RAB3IP2; RIM; RIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            4438..4604
                     /gene="RIMS1"
                     /gene_synonym="CORD7; RAB3IP2; RIM; RIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            4605..4840
                     /gene="RIMS1"
                     /gene_synonym="CORD7; RAB3IP2; RIM; RIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            4841..4979
                     /gene="RIMS1"
                     /gene_synonym="CORD7; RAB3IP2; RIM; RIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            4980..5092
                     /gene="RIMS1"
                     /gene_synonym="CORD7; RAB3IP2; RIM; RIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            5093..5194
                     /gene="RIMS1"
                     /gene_synonym="CORD7; RAB3IP2; RIM; RIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            5195..5334
                     /gene="RIMS1"
                     /gene_synonym="CORD7; RAB3IP2; RIM; RIM1"
                     /inference="alignment:Splign:2.1.0"
     exon            5335..7984
                     /gene="RIMS1"
                     /gene_synonym="CORD7; RAB3IP2; RIM; RIM1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7623..7628
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RIMS1"
                     /gene_synonym="CORD7; RAB3IP2; RIM; RIM1"
                     /note="hexamer: AATAAA"
     polyA_site      7645
                     /gene="RIMS1"
                     /gene_synonym="CORD7; RAB3IP2; RIM; RIM1"
                     /note="major polyA site"
     regulatory      7961..7966
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RIMS1"
                     /gene_synonym="CORD7; RAB3IP2; RIM; RIM1"
                     /note="hexamer: AATAAA"
     polyA_site      7984
                     /gene="RIMS1"
                     /gene_synonym="CORD7; RAB3IP2; RIM; RIM1"
                     /note="major polyA site"
ORIGIN      
        1 aaattggagg cagcagagcc tgggagcagc ctccacggcg gcagcggccg ccccagtccc
       61 agccccagcc ccagccccag ccccgccccc gccccgcgcc ccgccggaga cgcccggatc
      121 ggcggagcct ggcgcgagcc ctcgccccct cgcctctccc gccgcgcctc cgcctgcccg
      181 cccccgccgg ccgaggctgg gctgcgggag gcggccgggc ggccccgagc ttcgctaggg
      241 cgaccaaaac aaaggcagca tccggggctg ggtggatgca aacaaccatg aaagactggg
      301 ttctcgctct ccccggctct gctgctgctg ctgctgccgc cgccgccgct gctcctcctc
      361 ctgccgccgc cgctagggct ccgctgtgag ggggaagcag gggcgcagct gctgggcgtg
      421 catccgaaag gtgagagcca gagagcgagc agagggggcg ggcaggccac gaaaatgtcc
      481 tcggccgtgg ggccccgcgg tcctcgccca cccacggtgc ctccccccat gcaagagctg
      541 cccgacctga gccacctgac cgaagaggag aggaacatta tcatggcagt gatggaccgg
      601 cagaaggaag aggaggaaaa agaagaagcc atgctcaagt gtgttgtcag ggacatggcg
      661 aagcctgctg cctgcaaaac accaagaaat gctgaaaacc agccccacca accttcaccg
      721 agattgcatc aacagtttga aagctataag gaacaagtga gaaaaatagg ggaagaagcg
      781 cggcgttacc agggcgagca caaagacgat gctccgactt gtggaatctg tcataaaaca
      841 aagtttgctg atgggtgcgg tcatctctgc tcctattgtc gcactaagtt ctgtgcgcgc
      901 tgcggaggcc gcgtgtctct acggtcaaac aacgaggaca aagtggttat gtgggtatgc
      961 aatttatgtc gaaagcaaca agaaatctta accaaatctg gggcatggtt ctttggaagt
     1021 ggccctcagc agacaagtca ggatggaacc ctgagtgata cagctacagg tgctggctct
     1081 gaggtaccaa gagaaaagaa agcacgactc caagagcgat cgcggtctca gacaccccta
     1141 agcacagcag ctgcctcctc ccaggatgct gctcctccca gcgcaccacc agacaggagc
     1201 aaaggggctg agccctcgca gcaagccttg gggcctgaac agaagcaggc ttcatccagg
     1261 tctagaagtg aacctcctag agagagaaag aagaccccag ggctttccga gcagaatggc
     1321 aaaggagccc tgaagagcga gcggaaacgc gtgccaaaga cctcagcgca gcccgtggag
     1381 ggggccgtcg aagaacggga gcgcaaagaa aggcgggaaa gccgaaggct tgagaaaggg
     1441 cgatcacagg attacccaga cacgccggaa aaacgggatg agggcaaagc ggcggatgag
     1501 gaaaagcaaa gaaaagagga ggattatcag accaggtacc gcagcgaccc gaacctagct
     1561 cggtacccgg tgaaaccgcc gcctgaggag cagcagatgc gcatgcacgc ccgggtgtcc
     1621 cgcgccaggc acgagcggcg ccacagcgac gtggcgctcc cgcgcaccga ggcgggcgcg
     1681 gcgctgccgg agggcaaggc cggcaaacgc gcgccggcgg cagccagggc ctcgccgccg
     1741 gactcgccgc gggcttactc ggctgagaga actgcggaga ccagggcgcc gggcgccaag
     1801 cagctaacga accacagccc gccggcgccc agacatgggc cggttcccgc agaagccccg
     1861 gagctcaaag cccaggagcc cctcaggaag cagagccgcc tggaccccag ctcggcggtc
     1921 ctcatgcgga aggccaagcg cgagaaggtg gagaccatgc tgcggaacga ctctttgagc
     1981 tcagaccagt ccgagtcggt gcggccgtcc ccgcccaagc cgcaccggtc caagagaggc
     2041 ggcaagaagc ggcagatgtc ggtgagcagc tctgaggagg agggcgtgtc gacgcccgag
     2101 tacaccagct gcgaggacgt ggagctggag agcgagagcg tcagcgagaa aggtgatttg
     2161 gattattact ggttggatcc tgccacgtgg cacagccggg agacatcacc tattagttcg
     2221 catcctgtaa cgtggcaacc atctaaagag ggggaccgat taattggacg tgttattctt
     2281 aacaagagaa caaccatgcc caaagactca ggtgcattgc tgggtctgaa agttgttgga
     2341 ggaaaaatga ctgacttagg acgacttggt gctttcatca ccaaagtaaa gaagggtagc
     2401 ctagcagatg tagttggaca cctaagagca ggggatgaag ttctagaatg gaatggtaaa
     2461 cccctgccgg gagctacaaa tgaagaagtt tacaacatta ttttagaatc aaaatcagaa
     2521 cctcaagttg aaattattgt ttcaaggcct attggtgaca ttccccggat tcctgagagc
     2581 tcccaccctc cactggagtc cagttcaagt tcctttgaat ctcagaagat ggaaaggcct
     2641 tccatttctg ttatttctcc aacaagtcct ggagctctaa aagatgcccc acaagtctta
     2701 ccagggcaac tttctgtgaa gttgtggtat gataaagtgg gacaccagct gattgtaaat
     2761 gttctgcaag caacagatct acctgctaga gtagatggac gtcctcgaaa tccctatgta
     2821 aaaatgtatt ttcttccaga tagaagtgat aaaagtaaaa ggaggaccaa aacagtaaag
     2881 aaaatactag aaccaaaatg gaatcaaact tttgtctatt cacatgtaca tcgtagagat
     2941 tttagagaac gaatgttaga aataactgtg tgggaccaac caagagtgca agaagaagaa
     3001 agtgaatttc ttggagagat cctcatagaa ttggagacag cgcttttaga tgatgaaccg
     3061 cattggtata aacttcagac acatgatgag tcttcactac ctctgcctca gccatcacct
     3121 ttcatgccaa ggcgacatat tcatggagaa agctctagca aaaagctaca aagatctcag
     3181 cgaatcagtg atagtgacat ctcagattat gaggttgatg atggtattgg cgtagttcct
     3241 ccagtaggct ataggtctag tgctagagaa agtaaatcta caacattaac tgtgccagaa
     3301 cagcaaagaa caactcatca ccgctcacgt tcagtatctc ctcatcgcgg caatgatcag
     3361 ggaaagccgc gttcacgttt accaaatgtg ccattacaga ggagtttaga tgaaattcat
     3421 ccaacaagaa ggtcacgttc tccaaccaga caccatgatg cctcccgaag tccagttgat
     3481 catagaacca gagatgtgga tagtcagtat ttatcagaac aagacagtga gcttcttatg
     3541 ctgcccagag caaaacgagg acgaagtgca gaatgcctac atactaccag acatcttgtt
     3601 aggcactata aaacattacc tcccaagatg cctttattac agagcagttc tcactggaat
     3661 atttacagct caattctgcc tgcacatact aagaccaaat cagtgactag acaggacatt
     3721 tcccttcatc atgaatgctt taactcaaca gtattgagat ttactgatga aatactggtt
     3781 agtgaactgc agccctttct tgacagggct aggagtgcta gtaccaactg cttgagacca
     3841 gatactagtt tgcattcacc agaacgagaa aggggtagat ggtccccctc cctagatagg
     3901 agacgacctc ctagtcccag gattcaaatc cagcatgcgt ctccggagaa tgacaggcac
     3961 tccagaaagt ctgaaagatc tagcatccaa aaacagacta ggaaaggcac tgcctctgat
     4021 gcagaaaggg ttctcccaac atgtctttct agaaggggac acgcagcccc aagagcaact
     4081 gatcagccag tcattagggg aaaacatcct gctcgctcaa ggtcgagtga gcactctagt
     4141 atcagaacac tgtgttctat gcaccacctt gtccctggag ggtcggcgcc accttctccg
     4201 cttctgacaa gaatgcaccg acagagaagt ccaacacaat ctcctccagc agacacatcg
     4261 ttcagcagtc gcaggggaag acagctccca caagtgccag tgagaagcgg cagtatagaa
     4321 caagcaagct tagtagtgga ggagcgaaca agacagatga aaatgaaagt gcatcgattt
     4381 aagcagacaa cagggtctgg ttctagtcaa gaacttgatc gcgagcaata ttccaagtat
     4441 aacatacata aagatcagta cagaagctgt gataacgtct ctgccaaatc atcagatagt
     4501 gatgtcagtg atgtttccgc catttcccga accagcagtg cctcacgcct cagcagcaca
     4561 agctttatgt cagagcaatc tgagcgcccc aggggtagaa tcagttcatt tacccccaaa
     4621 atgcaaggca gacggatggg gacttcagga agatccatca tgaagagcac cagtgtcagt
     4681 ggagagatgt acacactgga gcataatgac ggcagccagt cagacacagc tgtgggtaca
     4741 gttggagcag gtggaaagaa acggagatcc agccttagtg ccaaagtggt tgccatagtg
     4801 tctcgaagga gtagaagcac atcccagctt agtcaaacag agtcgggcca caaaaagtta
     4861 aaaagtacca tccagagaag cacagaaaca ggcatggcag ctgaaatgag aaagatggta
     4921 aggcagccga gccgagagtc tactgatggc agcatcaaca gttacagctc tgagggcaat
     4981 ttaatatttc ctggagtgcg actgggagct gacagtcaat tcagtgattt tcttgatgga
     5041 ttgggaccag cccagcttgt tggccgccaa acccttgcca cccctgcaat gggtgatata
     5101 caaataggaa tggaggacaa aaagggccaa ttagaagtgg aagtcattag agcacgaagc
     5161 ctcacacaaa agcctggttc caaatctaca cctgctccat atgtcaaagt atatcttttg
     5221 gaaaatgggg cctgtatagc caagaagaag acaagaattg cacgaaaaac ccttgatcct
     5281 ttgtatcagc agtctctggt ttttgatgaa agtccacagg gtaaagttct tcaggtgatt
     5341 gtctggggag actatggcag aatggaccac aaatgcttta tgggtgtggc tcagatcttg
     5401 ttggaagaac tcgacctgtc cagcatggtg atcggatggt acaaattgtt cccaccgtcc
     5461 tcactggtgg atcccacact cactcccctc acccggcggg cttcccagtc atctctggaa
     5521 agttcaactg ggcctccctg tattcgatca tagtgaactc ataccagagt cattccaata
     5581 aaactctact tttcaggata ataatctgaa ccagatattt catgatcgaa agcattgttg
     5641 gagacagaca atcaacttgt gttttgcctg tagtagtttt tcaataatat gtcccaattg
     5701 ttatttaaaa catggcttca tatgacagaa caaggcaatc tatcaaattt acaggaagaa
     5761 tcaacatgct ggtgagagtc actgatgctt ctaacaaata gaaaaagagg aaactttaaa
     5821 tccacgcata cacgtacaca cacacatgca cacacacaca caccaaattg aacaaactgg
     5881 aaactctcac tctgtgaaaa gttgtattct acacatttct gcacagacca caagcagtgt
     5941 tatttccctg atgtttgagt tgttttgttc ttttgtgtgt tttgtttatt tgtgtgttgt
     6001 ttgttggttt tcgtttcttg agtttttgtt ttgttttcac cacatctgtt atttccacta
     6061 gttttttttt ggctgtgtca tgacaggcct catgatgcta acagagattc ttcccttctt
     6121 ttttccaaaa gcaccaaaaa aaagggctga agtagtctct gtagcatctc cccaaagtgt
     6181 taaacagcta cataaggtga agccaaggaa gggtttcact agctttcatt ttataattat
     6241 ttaaagtctt atgtgtggat acccaagaga caaaaagtta tcttccaaaa taaataatca
     6301 cagaagtact tttttaatga agagcctatc tgtttgcatt ctagaaaatt aatttatggt
     6361 ttccattgtt ttgcatggaa gacttttaaa gaagtcaatc tgcaactaat gctggggact
     6421 aaaactaact gcataattgt actttgaaaa cactccttgt aatgcctttt tcttcctgat
     6481 attaaaaaaa tgctcatgtg tataaaccat actttgactt cccaaaaatg gtgactagga
     6541 atggatttga gcctaagaca ctttgagcta tgcccaggct tctcagccat acctcagtgt
     6601 agacacatcc atctgccctg gaaagagcat ggtatgagct gttcaatatt ccactggaaa
     6661 ttccagttga aatattctgc actaaactat tgtttttatt gaattttagt ttacatttct
     6721 ttgagattga tgcaagcaca aagcttgatt ttttaatgca aagtatctta ctttttgggg
     6781 ggaaaataaa caaaatgaac tttcaatttg cattttcatt tagtttctct tggccttgaa
     6841 tttcccttgt gacattctgt acatgtgcta caaactgcag tctctgcagg tgcattgata
     6901 tgttctccct ccttttatga gtcactccac ttttgtgatt acacaaatag agcagcatga
     6961 cttggaactg ttcagagctg catgcacatt ttgtaaaaaa aaaaaatttt aaaaagaaaa
     7021 gaaacaaaag gttatttaat aatgtatgtt agttccacat aggccagctt gtatgttgca
     7081 tgtacttgta cagtttgctt gttaattact ataccgaaat aaccaagaaa tctaagccat
     7141 ccacttttgt tatagacatt ttgcaggttt tagccagact cagtctctgg gtctgtggtg
     7201 aaatgtctat agatggactt tattttttac cctccggaaa acgtgatgta gtacggacca
     7261 aattccttct aataaatatt ttggtgtaga ttcctactgt ggggctgtaa cacatgtaga
     7321 cactgtgtac acactaggtt ttaattcata gacatactgc ctgcaccaag tgaattattt
     7381 attattattt acacatgcca gaattatctg cccatcattc cacagggcac agattgacca
     7441 ctgctcacca tgctgagtag gaggaactga agcattggtg cacttctact agattccgtt
     7501 ctgtcttagt ggccaacaat caactgatta taattgggta gtatctttct attttgacca
     7561 cttgtcttaa cactcccctg tgcttttaaa tgaaattcca attcatgtat gtaaacatgt
     7621 ttaataaaat ttacttttca tgtcagtcag tagccatatg tgttctatgt cttgccagat
     7681 ttccattgtg ggttctatgg tgcaaaatgg gagccaggcc atgggaaggg gaggctgggg
     7741 tttgttgtct gctgcgggca tttgacttga cttgtcactg cactatatta tgtcttaatc
     7801 gatggcccaa agggtacttt gcatcacata caagttttca cttcgtcagc ctgtctaagt
     7861 gagcacatga ctatacttct gaaaagtggc attattaaaa ataataaatc tcagtaagaa
     7921 tcaaatggaa ggttgttttc tgctgctgtt tgaatgtgct aataaaagag acaactctat
     7981 tgtc
//
