LOCUS       NM_014314               4628 bp    mRNA    linear   PRI 10-DEC-2024
DEFINITION  Homo sapiens RNA sensor RIG-I (RIGI), transcript variant 1, mRNA.
ACCESSION   NM_014314
VERSION     NM_014314.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Qiao,Y., Zhu,S., Yang,N., Zou,S.S., Gao,B., Wu,J., Liu,C., Li,X.,
            Liu,Y.J. and Chen,J.
  TITLE     The RNA helicase DHX35 functions as a co-sensor for RIG-I-mediated
            innate immunity
  JOURNAL   PLoS Pathog 20 (7), e1012379 (2024)
   PUBMED   39037956
  REMARK    GeneRIF: The RNA helicase DHX35 functions as a co-sensor for
            RIG-I-mediated innate immunity.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   Solotchi,M. and Patel,S.S.
  TITLE     Proofreading mechanisms of the innate immune receptor RIG-I:
            distinguishing self and viral RNA
  JOURNAL   Biochem Soc Trans 52 (3), 1131-1148 (2024)
   PUBMED   38884803
  REMARK    GeneRIF: Proofreading mechanisms of the innate immune receptor
            RIG-I: distinguishing self and viral RNA.
            Review article
REFERENCE   3  (bases 1 to 4628)
  AUTHORS   Wu,B., Li,D., Bai,H., Mo,R., Li,H., Xie,J., Zhang,X., Yang,Y.,
            Li,H., Idris,A., Li,X. and Feng,R.
  TITLE     Mammalian reovirus micro1 protein attenuates RIG-I and
            MDA5-mediated signaling transduction by blocking IRF3
            phosphorylation and nuclear translocation
  JOURNAL   Mol Immunol 170, 131-143 (2024)
   PUBMED   38663254
  REMARK    GeneRIF: Mammalian reovirus micro1 protein attenuates RIG-I and
            MDA5-mediated signaling transduction by blocking IRF3
            phosphorylation and nuclear translocation.
REFERENCE   4  (bases 1 to 4628)
  AUTHORS   Huang,X., Zhou,X., Zhang,F., Wang,X., Duan,X. and Liu,K.
  TITLE     DDX58 variant triggers IFN-beta-induced autophagy in trabecular
            meshwork and influences intraocular pressure
  JOURNAL   FASEB J 38 (10), e23651 (2024)
   PUBMED   38752537
  REMARK    GeneRIF: DDX58 variant triggers IFN-beta-induced autophagy in
            trabecular meshwork and influences intraocular pressure.
REFERENCE   5  (bases 1 to 4628)
  AUTHORS   Chen,J., Wang,Q., Zhong,B., Zheng,H., Wang,D., Huang,X., Liu,L. and
            Liu,T.
  TITLE     Activation of the RIG-I/MAVS Signaling Pathway during Human
            Adenovirus Type 3 Infection Impairs the Pro-Inflammatory Response
            Induced by Secondary Infection with Staphylococcus aureus
  JOURNAL   Int J Mol Sci 25 (8), 4178 (2024)
   PUBMED   38673764
  REMARK    GeneRIF: Activation of the RIG-I/MAVS Signaling Pathway during
            Human Adenovirus Type 3 Infection Impairs the Pro-Inflammatory
            Response Induced by Secondary Infection with Staphylococcus aureus.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4628)
  AUTHORS   Imaizumi,T., Yagihashi,N., Hatakeyama,M., Yamashita,K.,
            Ishikawa,A., Taima,K., Yoshida,H., Inoue,I., Fujita,T.,
            Yagihashi,S. and Satoh,K.
  TITLE     Expression of retinoic acid-inducible gene-I in vascular smooth
            muscle cells stimulated with interferon-gamma
  JOURNAL   Life Sci 75 (10), 1171-1180 (2004)
   PUBMED   15219805
  REMARK    GeneRIF: RIG-I may play some pathophysiological role in immune and
            inflammatory reactions in vascular smooth muscle cells(SMC).
REFERENCE   7  (bases 1 to 4628)
  AUTHORS   Yoneyama,M., Kikuchi,M., Natsukawa,T., Shinobu,N., Imaizumi,T.,
            Miyagishi,M., Taira,K., Akira,S. and Fujita,T.
  TITLE     The RNA helicase RIG-I has an essential function in double-stranded
            RNA-induced innate antiviral responses
  JOURNAL   Nat Immunol 5 (7), 730-737 (2004)
   PUBMED   15208624
  REMARK    GeneRIF: RIG-I is key in the detection and subsequent eradication
            of the replicating viral genomes
REFERENCE   8  (bases 1 to 4628)
  AUTHORS   Cui,X.F., Imaizumi,T., Yoshida,H., Borden,E.C. and Satoh,K.
  TITLE     Retinoic acid-inducible gene-I is induced by interferon-gamma and
            regulates the expression of interferon-gamma stimulated gene 15 in
            MCF-7 cells
  JOURNAL   Biochem Cell Biol 82 (3), 401-405 (2004)
   PUBMED   15181474
  REMARK    GeneRIF: IFN-gamma induces the expression of RIG-I, which may play
            a role in the immunological effects of IFN-gamma
REFERENCE   9  (bases 1 to 4628)
  AUTHORS   Imaizumi,T., Aratani,S., Nakajima,T., Carlson,M., Matsumiya,T.,
            Tanji,K., Ookawa,K., Yoshida,H., Tsuchida,S., McIntyre,T.M.,
            Prescott,S.M., Zimmerman,G.A. and Satoh,K.
  TITLE     Retinoic acid-inducible gene-I is induced in endothelial cells by
            LPS and regulates expression of COX-2
  JOURNAL   Biochem Biophys Res Commun 292 (1), 274-279 (2002)
   PUBMED   11890704
REFERENCE   10 (bases 1 to 4628)
  AUTHORS   Chaudhary,P.M., Eby,M.T., Jasmin,A., Kumar,A., Liu,L. and Hood,L.
  TITLE     Activation of the NF-kappaB pathway by caspase 8 and its homologs
  JOURNAL   Oncogene 19 (39), 4451-4460 (2000)
   PUBMED   11002417
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DN998145.1, AF038963.1,
            BC070029.1, AL161783.20 and AA862613.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_014314.3.
            
            Summary: DEAD box proteins, characterized by the conserved motif
            Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are
            implicated in a number of cellular processes involving RNA binding
            and alteration of RNA secondary structure. This gene encodes a
            protein containing RNA helicase-DEAD box protein motifs and a
            caspase recruitment domain (CARD). It is involved in viral
            double-stranded (ds) RNA recognition and the regulation of the
            antiviral innate immune response. Mutations in this gene are
            associated with Singleton-Merten syndrome 2. [provided by RefSeq,
            Aug 2020].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC136610.1, SRR18074969.2647065.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            coronavirus related    :: involved in immune response or antiviral
                                      activity
            MANE Ensembl match     :: ENST00000379883.3/ ENSP00000369213.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-525               DN998145.1         130-654
            526-1769            AF038963.1         653-1896
            1770-1804           BC070029.1         1635-1669
            1805-2429           AF038963.1         1932-2556
            2430-3439           BC070029.1         2290-3299
            3440-4609           AL161783.20        102967-104136       c
            4610-4628           AA862613.1         4-22                c
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9p21.1"
     gene            1..4628
                     /gene="RIGI"
                     /gene_synonym="DDX58; RIG-I; RIG1; RLR-1; SGMRT2"
                     /note="RNA sensor RIG-I"
                     /db_xref="GeneID:23586"
                     /db_xref="HGNC:HGNC:19102"
                     /db_xref="MIM:609631"
     exon            1..136
                     /gene="RIGI"
                     /gene_synonym="DDX58; RIG-I; RIG1; RLR-1; SGMRT2"
                     /inference="alignment:Splign:2.1.0"
     CDS             31..2808
                     /gene="RIGI"
                     /gene_synonym="DDX58; RIG-I; RIG1; RLR-1; SGMRT2"
                     /EC_number="3.6.4.13"
                     /note="isoform 1 is encoded by transcript variant 1; RNA
                     helicase RIG-I; DEAD/H (Asp-Glu-Ala-Asp/His) box
                     polypeptide; probable ATP-dependent RNA helicase DDX58;
                     DEAD box protein 58; DEAD (Asp-Glu-Ala-Asp) box
                     polypeptide 58; retinoic acid-inducible gene 1 protein;
                     retinoic acid-inducible gene I protein; antiviral innate
                     immune response receptor RIG-I; DExD/H-box helicase 58"
                     /codon_start=1
                     /product="antiviral innate immune response receptor RIG-I
                     isoform 1"
                     /protein_id="NP_055129.2"
                     /db_xref="CCDS:CCDS6526.1"
                     /db_xref="GeneID:23586"
                     /db_xref="HGNC:HGNC:19102"
                     /db_xref="MIM:609631"
                     /translation="MTTEQRRSLQAFQDYIRKTLDPTYILSYMAPWFREEEVQYIQAE
                     KNNKGPMEAATLFLKFLLELQEEGWFRGFLDALDHAGYSGLYEAIESWDFKKIEKLEE
                     YRLLLKRLQPEFKTRIIPTDIISDLSECLINQECEEILQICSTKGMMAGAEKLVECLL
                     RSDKENWPKTLKLALEKERNKFSELWIVEKGIKDVETEDLEDKMETSDIQIFYQEDPE
                     CQNLSENSCPPSEVSDTNLYSPFKPRNYQLELALPAMKGKNTIICAPTGCGKTFVSLL
                     ICEHHLKKFPQGQKGKVVFFANQIPVYEQQKSVFSKYFERHGYRVTGISGATAENVPV
                     EQIVENNDIIILTPQILVNNLKKGTIPSLSIFTLMIFDECHNTSKQHPYNMIMFNYLD
                     QKLGGSSGPLPQVIGLTASVGVGDAKNTDEALDYICKLCASLDASVIATVKHNLEELE
                     QVVYKPQKFFRKVESRISDKFKYIIAQLMRDTESLAKRICKDLENLSQIQNREFGTQK
                     YEQWIVTVQKACMVFQMPDKDEESRICKALFLYTSHLRKYNDALIISEHARMKDALDY
                     LKDFFSNVRAAGFDEIEQDLTQRFEEKLQELESVSRDPSNENPKLEDLCFILQEEYHL
                     NPETITILFVKTRALVDALKNWIEGNPKLSFLKPGILTGRGKTNQNTGMTLPAQKCIL
                     DAFKASGDHNILIATSVADEGIDIAQCNLVILYEYVGNVIKMIQTRGRGRARGSKCFL
                     LTSNAGVIEKEQINMYKEKMMNDSILRLQTWDEAVFREKILHIQTHEKFIRDSQEKPK
                     PVPDKENKKLLCRKCKALACYTADVRVIEECHYTVLGDAFKECFVSRPHPKPKQFSSF
                     EKRAKIFCARQNCSHDWGIHVKYKTFEIPVIKIESFVVEDIATGVQTLYSKWKDFHFE
                     KIPFDPAEMSK"
     exon            137..271
                     /gene="RIGI"
                     /gene_synonym="DDX58; RIG-I; RIG1; RLR-1; SGMRT2"
                     /inference="alignment:Splign:2.1.0"
     exon            272..453
                     /gene="RIGI"
                     /gene_synonym="DDX58; RIG-I; RIG1; RLR-1; SGMRT2"
                     /inference="alignment:Splign:2.1.0"
     exon            454..601
                     /gene="RIGI"
                     /gene_synonym="DDX58; RIG-I; RIG1; RLR-1; SGMRT2"
                     /inference="alignment:Splign:2.1.0"
     exon            602..721
                     /gene="RIGI"
                     /gene_synonym="DDX58; RIG-I; RIG1; RLR-1; SGMRT2"
                     /inference="alignment:Splign:2.1.0"
     exon            722..829
                     /gene="RIGI"
                     /gene_synonym="DDX58; RIG-I; RIG1; RLR-1; SGMRT2"
                     /inference="alignment:Splign:2.1.0"
     exon            830..983
                     /gene="RIGI"
                     /gene_synonym="DDX58; RIG-I; RIG1; RLR-1; SGMRT2"
                     /inference="alignment:Splign:2.1.0"
     exon            984..1242
                     /gene="RIGI"
                     /gene_synonym="DDX58; RIG-I; RIG1; RLR-1; SGMRT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1243..1405
                     /gene="RIGI"
                     /gene_synonym="DDX58; RIG-I; RIG1; RLR-1; SGMRT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1406..1510
                     /gene="RIGI"
                     /gene_synonym="DDX58; RIG-I; RIG1; RLR-1; SGMRT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1511..1668
                     /gene="RIGI"
                     /gene_synonym="DDX58; RIG-I; RIG1; RLR-1; SGMRT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1669..1804
                     /gene="RIGI"
                     /gene_synonym="DDX58; RIG-I; RIG1; RLR-1; SGMRT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1805..1953
                     /gene="RIGI"
                     /gene_synonym="DDX58; RIG-I; RIG1; RLR-1; SGMRT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1954..2044
                     /gene="RIGI"
                     /gene_synonym="DDX58; RIG-I; RIG1; RLR-1; SGMRT2"
                     /inference="alignment:Splign:2.1.0"
     exon            2045..2215
                     /gene="RIGI"
                     /gene_synonym="DDX58; RIG-I; RIG1; RLR-1; SGMRT2"
                     /inference="alignment:Splign:2.1.0"
     exon            2216..2367
                     /gene="RIGI"
                     /gene_synonym="DDX58; RIG-I; RIG1; RLR-1; SGMRT2"
                     /inference="alignment:Splign:2.1.0"
     exon            2368..2511
                     /gene="RIGI"
                     /gene_synonym="DDX58; RIG-I; RIG1; RLR-1; SGMRT2"
                     /inference="alignment:Splign:2.1.0"
     exon            2512..4628
                     /gene="RIGI"
                     /gene_synonym="DDX58; RIG-I; RIG1; RLR-1; SGMRT2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2909..2914
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RIGI"
                     /gene_synonym="DDX58; RIG-I; RIG1; RLR-1; SGMRT2"
                     /note="hexamer: AATATA"
     polyA_site      2927
                     /gene="RIGI"
                     /gene_synonym="DDX58; RIG-I; RIG1; RLR-1; SGMRT2"
     regulatory      4201..4206
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RIGI"
                     /gene_synonym="DDX58; RIG-I; RIG1; RLR-1; SGMRT2"
                     /note="hexamer: AATACA"
     polyA_site      4225
                     /gene="RIGI"
                     /gene_synonym="DDX58; RIG-I; RIG1; RLR-1; SGMRT2"
                     /note="major polyA site"
     polyA_site      4628
                     /gene="RIGI"
                     /gene_synonym="DDX58; RIG-I; RIG1; RLR-1; SGMRT2"
ORIGIN      
        1 gaacgtagct agctgcaagc agaggccggc atgaccaccg agcagcgacg cagcctgcaa
       61 gccttccagg attatatccg gaagaccctg gaccctacct acatcctgag ctacatggcc
      121 ccctggttta gggaggaaga ggtgcagtat attcaggctg agaaaaacaa caagggccca
      181 atggaggctg ccacactttt tctcaagttc ctgttggagc tccaggagga aggctggttc
      241 cgtggctttt tggatgccct agaccatgca ggttattctg gactttatga agccattgaa
      301 agttgggatt tcaaaaaaat tgaaaagttg gaggagtata gattactttt aaaacgttta
      361 caaccagaat ttaaaaccag aattatccca accgatatca tttctgatct gtctgaatgt
      421 ttaattaatc aggaatgtga agaaattcta cagatttgct ctactaaggg gatgatggca
      481 ggtgcagaga aattggtgga atgccttctc agatcagaca aggaaaactg gcccaaaact
      541 ttgaaacttg ctttggagaa agaaaggaac aagttcagtg aactgtggat tgtagagaaa
      601 ggtataaaag atgttgaaac agaagatctt gaggataaga tggaaacttc tgacatacag
      661 attttctacc aagaagatcc agaatgccag aatcttagtg agaattcatg tccaccttca
      721 gaagtgtctg atacaaactt gtacagccca tttaaaccaa gaaattacca attagagctt
      781 gctttgcctg ctatgaaagg aaaaaacaca ataatatgtg ctcctacagg ttgtggaaaa
      841 acctttgttt cactgcttat atgtgaacat catcttaaaa aattcccaca aggacaaaag
      901 gggaaagttg tcttttttgc gaatcagatc ccagtgtatg aacagcagaa atctgtattc
      961 tcaaaatact ttgaaagaca tgggtataga gttacaggca tttctggagc aacagctgag
     1021 aatgtcccag tggaacagat tgttgagaac aatgacatca tcattttaac tccacagatt
     1081 cttgtgaaca accttaaaaa gggaacgatt ccatcactat ccatctttac tttgatgata
     1141 tttgatgaat gccacaacac tagtaaacaa cacccgtaca atatgatcat gtttaattat
     1201 ctagatcaga aacttggagg atcttcaggc ccactgcccc aggtcattgg gctgactgcc
     1261 tcggttggtg ttggggatgc caaaaacaca gatgaagcct tggattatat ctgcaagctg
     1321 tgtgcttctc ttgatgcgtc agtgatagca acagtcaaac acaatctgga ggaactggag
     1381 caagttgttt ataagcccca gaagtttttc aggaaagtgg aatcacggat tagcgacaaa
     1441 tttaaataca tcatagctca gctgatgagg gacacagaga gtctggcaaa gagaatctgc
     1501 aaagacctcg aaaacttatc tcaaattcaa aatagggaat ttggaacaca gaaatatgaa
     1561 caatggattg ttacagttca gaaagcatgc atggtgttcc agatgccaga caaagatgaa
     1621 gagagcagga tttgtaaagc cctgttttta tacacttcac atttgcggaa atataatgat
     1681 gccctcatta tcagtgagca tgcacgaatg aaagatgctc tggattactt gaaagacttc
     1741 ttcagcaatg tccgagcagc aggattcgat gagattgagc aagatcttac tcagagattt
     1801 gaagaaaagc tgcaggaact agaaagtgtt tccagggatc ccagcaatga gaatcctaaa
     1861 cttgaagacc tctgcttcat cttacaagaa gagtaccact taaacccaga gacaataaca
     1921 attctctttg tgaaaaccag agcacttgtg gacgctttaa aaaattggat tgaaggaaat
     1981 cctaaactca gttttctaaa acctggcata ttgactggac gtggcaaaac aaatcagaac
     2041 acaggaatga ccctcccggc acagaagtgt atattggatg cattcaaagc cagtggagat
     2101 cacaatattc tgattgccac ctcagttgct gatgaaggca ttgacattgc acagtgcaat
     2161 cttgtcatcc tttatgagta tgtgggcaat gtcatcaaaa tgatccaaac cagaggcaga
     2221 ggaagagcaa gaggtagcaa gtgcttcctt ctgactagta atgctggtgt aattgaaaaa
     2281 gaacaaataa acatgtacaa agaaaaaatg atgaatgact ctattttacg ccttcagaca
     2341 tgggacgaag cagtatttag ggaaaagatt ctgcatatac agactcatga aaaattcatc
     2401 agagatagtc aagaaaaacc aaaacctgta cctgataagg aaaataaaaa actgctctgc
     2461 agaaagtgca aagccttggc atgttacaca gctgacgtaa gagtgataga ggaatgccat
     2521 tacactgtgc ttggagatgc ttttaaggaa tgctttgtga gtagaccaca tcccaagcca
     2581 aagcagtttt caagttttga aaaaagagca aagatattct gtgcccgaca gaactgcagc
     2641 catgactggg gaatccatgt gaagtacaag acatttgaga ttccagttat aaaaattgaa
     2701 agttttgtgg tggaggatat tgcaactgga gttcagacac tgtactcgaa gtggaaggac
     2761 tttcattttg agaagatacc atttgatcca gcagaaatgt ccaaatgata tcaggtcctc
     2821 aatcttcagc tacagggaat gagtaacttt gagtggagaa gaaacaaaca tagtgggtat
     2881 aatcatggat cgcttgtacc cctgtgaaaa tatatttttt aaaaatatct ttagcagttt
     2941 gtactatatt atatatgcaa agcacaaatg agtgaatcac agcactgagt attttgtagg
     3001 ccaacagagc tcatagtact tgggaaaaat taaaaagcct catttctagc cttcttttta
     3061 gagtcaactg ccaacaaaca cacagtaatc actctgtaca cactgggata gatgaatgaa
     3121 tggaatgttg ggaattttta tctccctttg tctccttaac ctactgtaaa ctggcttttg
     3181 cccttaacaa tctactgaaa ttgttctttt gaaggttacc agtgactctg gttgccaaat
     3241 ccactgggca cttcttaacc ttctatttga cctctgcgca tttggccctg ttgagcactc
     3301 ttcttgaagc tctccctggg cttctctctc ttctagttct attctagtct ttttttattg
     3361 agtcctcctc tttgctgatc ccttccaagg gttcaatata tatacatgta tatactgtac
     3421 atatgtatat gtaactaata tacatacata caggtatgta tatgtaatgg ttatatgtac
     3481 tcatgttcct ggtgtagcaa cgtgtggtat ggctacacag agaacatgag aacataaagc
     3541 catttttatg cttactacta aaagctgtcc actgtagagt tgctgtatgt agcaatgtgt
     3601 atccactcta cagtggtcag cttttagtag agagcataaa aatgataaaa tacttcttga
     3661 aaacttagtt tactatacat cttgccctat taatatgttc tcttaacgtg tgccattgtt
     3721 ctctttgacc attttcctat aatgatgttg atgttcaaca cctggactga atgtctgttc
     3781 tcagatccct tggatgttac agatgaggca gtctgactgt cctttctact tgaaagatta
     3841 gaatatgtat ccaaatggca ttcacgtgtc acttagcaag gtttgctgat gcttcaaaga
     3901 gcttagtttg cggtttcctg gacgtggaaa caagtatctg agttccctgg agatcaacgg
     3961 gatgaggtgt tacagctgcc tccctcttca tgcaatctgg tgagcagtgg tgcaggcggg
     4021 gagccagaga aacttgccag ttatataact tctctttggc ttttcttcat ctgtaaaaca
     4081 aggataatac tgaactgtaa gggttagtgg agagttttta attaaaagaa tgtgtgaaaa
     4141 gtacatgaca cagtagttgc ttgataatag ttactagtag tagtattctt actaagaccc
     4201 aatacaaatg gattatttaa accaagttta tgagttggtt ttttttcatt ttctatttgt
     4261 attttattaa gagtgtcttt tcttatgtga ttttttttaa ttgctatttg atatggtttg
     4321 gctatatgtc cccacccaaa tctcatcttg aattataatc cccatgtgtc aagggaggga
     4381 cctgacggga ggtgattgga tcacgggggc agttgtcccc atgctgttct tgggatagtg
     4441 agttagttct catgagatct gatggtttta taagtgtttg acaattcctc ctttacacac
     4501 actctctctc tcatctgctg ccatgtaaga cttgcctgct tccccttctg ccatgattgt
     4561 aagtttcctg aggcctcctc agccatgtgg aactgtgaat ctattaagcc tcttttcttt
     4621 ataaatga
//
