LOCUS       NM_001285439            9605 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens RPTOR independent companion of MTOR complex 2
            (RICTOR), transcript variant 2, mRNA.
ACCESSION   NM_001285439 XM_005248277
VERSION     NM_001285439.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9605)
  AUTHORS   Wang,Y., Zuo,D., Huang,Z., Qiu,Y., Wu,Z., Liu,S., Zeng,Y., Qiu,Z.,
            He,W., Li,B., Yuan,Y., Niu,Y. and Qiu,J.
  TITLE     KLF4 Suppresses the Progression of Hepatocellular Carcinoma by
            Reducing Tumor ATP Synthesis through Targeting the Mir-206/RICTOR
            Axis
  JOURNAL   Int J Mol Sci 25 (13), 7165 (2024)
   PUBMED   39000273
  REMARK    GeneRIF: KLF4 Suppresses the Progression of Hepatocellular
            Carcinoma by Reducing Tumor ATP Synthesis through Targeting the
            Mir-206/RICTOR Axis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9605)
  AUTHORS   Ponzone,L., Audrito,V., Landi,C., Moiso,E., Levra Levron,C.,
            Ferrua,S., Savino,A., Vitale,N., Gasparrini,M., Avalle,L.,
            Vantaggiato,L., Shaba,E., Tassone,B., Saoncella,S., Orso,F.,
            Viavattene,D., Marina,E., Fiorilla,I., Burrone,G., Abili,Y.,
            Altruda,F., Bini,L., Deaglio,S., Defilippi,P., Menga,A., Poli,V.,
            Porporato,P.E., Provero,P., Raffaelli,N., Riganti,C., Taverna,D.,
            Cavallo,F. and Calautti,E.
  TITLE     RICTOR/mTORC2 downregulation in BRAFV600E melanoma cells promotes
            resistance to BRAF/MEK inhibition
  JOURNAL   Mol Cancer 23 (1), 105 (2024)
   PUBMED   38755661
  REMARK    GeneRIF: RICTOR/mTORC2 downregulation in BRAF[V600E] melanoma cells
            promotes resistance to BRAF/MEK inhibition.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9605)
  AUTHORS   Wang,C., Chen,Z., Yi,Y., Ding,Y., Xu,F., Kang,H., Lin,K., Shu,X.,
            Zhong,Z., Zhang,Z., Liu,J., Xu,Z., Liu,L., He,X., Chang,Y. and
            Zhao,Q.
  TITLE     RBM45 reprograms lipid metabolism promoting hepatocellular
            carcinoma via Rictor and ACSL1/ACSL4
  JOURNAL   Oncogene 43 (5), 328-340 (2024)
   PUBMED   38040804
  REMARK    GeneRIF: RBM45 reprograms lipid metabolism promoting hepatocellular
            carcinoma via Rictor and ACSL1/ACSL4.
REFERENCE   4  (bases 1 to 9605)
  AUTHORS   Wang,C., Kang,H., Yi,Y., Ding,Y., Wang,F., Luo,J., Ye,M., Hong,Y.,
            Xia,C., Yan,J., Liu,L., Liu,J., Zhong,Z., Zhang,Z., Zhao,Q. and
            Chang,Y.
  TITLE     Rictor mediates p53 deactivation to facilitate the malignant
            transformation of hepatocytes and promote hepatocarcinogenesis
  JOURNAL   J Transl Med 21 (1), 919 (2023)
   PUBMED   38110956
  REMARK    GeneRIF: Rictor mediates p53 deactivation to facilitate the
            malignant transformation of hepatocytes and promote
            hepatocarcinogenesis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 9605)
  AUTHORS   Sztankovics,D., Krencz,I., Moldvai,D., Danko,T., Nagy,A., Nagy,N.,
            Bedics,G., Rokusz,A., Papp,G., Tokes,A.M., Papay,J., Sapi,Z.,
            Dezso,K., Bodor,C. and Sebestyen,A.
  TITLE     Novel RICTOR amplification harbouring entities: FISH validation of
            RICTOR amplification in tumour tissue after next-generation
            sequencing
  JOURNAL   Sci Rep 13 (1), 19610 (2023)
   PUBMED   37949943
  REMARK    GeneRIF: Novel RICTOR amplification harbouring entities: FISH
            validation of RICTOR amplification in tumour tissue after
            next-generation sequencing.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 9605)
  AUTHORS   Sarbassov,D.D., Guertin,D.A., Ali,S.M. and Sabatini,D.M.
  TITLE     Phosphorylation and regulation of Akt/PKB by the rictor-mTOR
            complex
  JOURNAL   Science 307 (5712), 1098-1101 (2005)
   PUBMED   15718470
  REMARK    GeneRIF: results show show that target of rapamycin (TOR) kinase
            and its associated protein rictor are necessary for AKT/PKB Ser473
            phosphorylation and that a reduction in rictor or mammalian TOR
            (mTOR) expression inhibited an Akt/PKB effector
REFERENCE   7  (bases 1 to 9605)
  AUTHORS   Jacinto,E., Loewith,R., Schmidt,A., Lin,S., Ruegg,M.A., Hall,A. and
            Hall,M.N.
  TITLE     Mammalian TOR complex 2 controls the actin cytoskeleton and is
            rapamycin insensitive
  JOURNAL   Nat Cell Biol 6 (11), 1122-1128 (2004)
   PUBMED   15467718
REFERENCE   8  (bases 1 to 9605)
  AUTHORS   Beausoleil,S.A., Jedrychowski,M., Schwartz,D., Elias,J.E.,
            Villen,J., Li,J., Cohn,M.A., Cantley,L.C. and Gygi,S.P.
  TITLE     Large-scale characterization of HeLa cell nuclear phosphoproteins
  JOURNAL   Proc Natl Acad Sci U S A 101 (33), 12130-12135 (2004)
   PUBMED   15302935
REFERENCE   9  (bases 1 to 9605)
  AUTHORS   Sarbassov,D.D., Ali,S.M., Kim,D.H., Guertin,D.A., Latek,R.R.,
            Erdjument-Bromage,H., Tempst,P. and Sabatini,D.M.
  TITLE     Rictor, a novel binding partner of mTOR, defines a
            rapamycin-insensitive and raptor-independent pathway that regulates
            the cytoskeleton
  JOURNAL   Curr Biol 14 (14), 1296-1302 (2004)
   PUBMED   15268862
REFERENCE   10 (bases 1 to 9605)
  AUTHORS   Ohara,O., Nagase,T., Mitsui,G., Kohga,H., Kikuno,R., Hiraoka,S.,
            Takahashi,Y., Kitajima,S., Saga,Y. and Koseki,H.
  TITLE     Characterization of size-fractionated cDNA libraries generated by
            the in vitro recombination-assisted method
  JOURNAL   DNA Res 9 (2), 47-57 (2002)
   PUBMED   12056414
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC008964.6, AC109467.3 and AC026713.5.
            
            On Jun 1, 2019 this sequence version replaced NM_001285439.1.
            
            Summary: RICTOR and MTOR (FRAP1; MIM 601231) are components of a
            protein complex that integrates nutrient- and growth factor-derived
            signals to regulate cell growth (Sarbassov et al., 2004 [PubMed
            15268862]).[supplied by OMIM, Mar 2008].
            
            Transcript Variant: This variant (2) contains an additional
            in-frame coding exon compared to variant 1. The resulting longer
            isoform (2) has an internal protein segment not found in isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC144509.1, SRR14038197.1349972.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-71                AC008964.6         49126-49196
            72-119              AC008964.6         49367-49414
            120-217             AC008964.6         102389-102486
            218-282             AC008964.6         119903-119967
            283-414             AC008964.6         120859-120990
            415-478             AC008964.6         126643-126706
            479-605             AC109467.3         4675-4801
            606-775             AC109467.3         13714-13883
            776-843             AC109467.3         17100-17167
            844-911             AC109467.3         20146-20213
            912-994             AC109467.3         23791-23873
            995-1082            AC109467.3         27720-27807
            1083-1173           AC109467.3         28323-28413
            1174-1240           AC109467.3         28523-28589
            1241-1321           AC109467.3         29029-29109
            1322-1422           AC109467.3         30858-30958
            1423-1588           AC109467.3         32709-32874
            1589-1690           AC109467.3         33164-33265
            1691-1737           AC109467.3         33389-33435
            1738-1873           AC109467.3         35217-35352
            1874-2073           AC109467.3         35772-35971
            2074-2200           AC109467.3         36429-36555
            2201-2365           AC109467.3         36919-37083
            2366-2442           AC109467.3         37231-37307
            2443-2521           AC109467.3         38020-38098
            2522-2631           AC109467.3         40046-40155
            2632-2719           AC109467.3         40889-40976
            2720-2812           AC109467.3         42197-42289
            2813-2919           AC109467.3         42659-42765
            2920-3149           AC109467.3         43325-43554
            3150-4158           AC109467.3         45030-46038
            4159-4230           AC109467.3         46323-46394
            4231-4408           AC109467.3         48309-48486
            4409-4493           AC109467.3         49198-49282
            4494-4727           AC109467.3         50026-50259
            4728-4883           AC109467.3         50682-50837
            4884-5007           AC109467.3         51181-51304
            5008-5146           AC109467.3         52779-52917
            5147-6207           AC109467.3         53372-54432
            6208-9605           AC026713.5         1-3398
FEATURES             Location/Qualifiers
     source          1..9605
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5p13.1"
     gene            1..9605
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /note="RPTOR independent companion of MTOR complex 2"
                     /db_xref="GeneID:253260"
                     /db_xref="HGNC:HGNC:28611"
                     /db_xref="MIM:609022"
     exon            1..71
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /inference="alignment:Splign:2.1.0"
     CDS             23..5221
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /note="isoform 2 is encoded by transcript variant 2;
                     rapamycin-insensitive companion of mTOR; pianissimo;
                     TORC2-specific protein AVO3; AVO3 homolog"
                     /codon_start=1
                     /product="rapamycin-insensitive companion of mTOR isoform
                     2"
                     /protein_id="NP_001272368.1"
                     /db_xref="CCDS:CCDS68861.1"
                     /db_xref="GeneID:253260"
                     /db_xref="HGNC:HGNC:28611"
                     /db_xref="MIM:609022"
                     /translation="MAAIGRGRSLKNLRVRGRNDSGEENVPLDLTREPSDNLREILQN
                     VARLQGVSNMRKLGHLNNFTKLLCDIGHSEEKLGFHYEDIIICLRLALLNEAKEVRAA
                     GLRALRYLIQDSSILQKVLKLKVDYLIARCIDIQQSNEVERTQALRLVRKMITVNASL
                     FPSSVTNSLIAVGNDGLQERDRMVRACIAIICELALQNPEVVALRGGLNTILKNVIDC
                     QLSRINEALITTILHLLNHPKTRQYVRADVELERILAPYTDFHYRHSPDTAEGQLKED
                     REARFLASKMGIIATFRSWAGIINLCKPGNSGIQSLIGVLCIPNMEIRRGLLEVLYDI
                     FRLPLPVVTEEFIEALLSVDPGRFQDSWRLSDGFVAAEAKTILPHRARSRPDLMDNYL
                     ALILSAFIRNGLLEGLVEVITNSDDHISVRATILLGELLHMANTILPHSHSHHLHCLP
                     TLMNMAASFDIPKEKRLRASAALNCLKRFHEMKKRGPKPYSLHLDHIIQKAIATHQKR
                     DQYLRVQKDIFILKDTEEALLINLRDSQVLQHKENLEWNWNLIGTILKWPNVNLRNYK
                     DEQLHRFVRRLLYFYKPSSKLYANLDLDFAKAKQLTVVGCQFTEFLLESEEDGQGYLE
                     DLVKDIVQWLNASSGMKPERSLQNNGLLTTLSQHYFLFIGTLSCHPHGVKMLEKCSVF
                     QCLLNLCSLKNQDHLLKLTVSSLDYSRDGLARVILSKILTAATDACRLYATKHLRVLL
                     RANVEFFNNWGIELLVTQLHDKNKTISSEALDILDEACEDKANLHALIQMKPALSHLG
                     DKGLLLLLRFLSIPKGFSYLNERGYVAKQLEKWHREYNSKYVDLIEEQLNEALTTYRK
                     PVDGDNYVRRSNQRLQRPHVYLPIHLYGQLVHHKTGCHLLEVQNIITELCRNVRTPDL
                     DKWEEIKKLKASLWALGNIGSSNWGLNLLQEENVIPDILKLAKQCEVLSIRGTCVYVL
                     GLIAKTKQGCDILKCHNWDAVRHSRKHLWPVVPDDVEQLCNELSSIPSTLSLNSESTS
                     SRHNSESESVPSSMFILEDDRFGSSSTSTFFLDINEDTEPTFYDRSGPIKDKNSFPFF
                     ASSKLVKNRILNSLTLPNKKHRSSSDPKGGKLSSESKTSNRRIRTLTEPSVDFNHSDD
                     FTPISTVQKTLQLETSFMGNKHIEDTGSTPSIGENDLKFTKNFGTENHRENTSRERLV
                     VESSTSSHMKIRSQSFNTDTTTSGISSMSSSPSRETVGVDATTMDTDCGSMSTVVSTK
                     TIKTSHYLTPQSNHLSLSKSNSVSLVPPGSSHTLPRRAQSLKAPSIATIKSLADCNFS
                     YTSSRDAFGYATLKRLQQQRMHPSLSHSEALASPAKDVLFTDTITMKANSFESRLTPS
                     RIDFKKKHVGGIRSLRPTITNNLFRFMKALSYASLDKEDLLSPINQNTLQRSSSVRSM
                     VSSATYGGSDDYIGLALPVDINDIFQVKDIPYFQTKNIPPHDDRGARAFAHDAGGLPS
                     GTGGLVKNSFHLLRQQMSLTEIMNSIHSDASLFLESTEDTGLQEHTDDNCLYCVCIEI
                     LGFQPSNQLSAICSHSDFQDIPYSDWCEQTIHNPLEVVPSKFSGISGCSDGVSQEGSA
                     SSTKSTELLLGVKTIPDDTPMCRILLRKEVLRLVINLSSSVSTKCHETGLLTIKEKYP
                     QTFDDICLYSEVSHLLSHCTFRLPCRRFIQELFQDVQFLQMHEEAEAVLATPPKQPIV
                     DTSAES"
     exon            72..119
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /inference="alignment:Splign:2.1.0"
     exon            120..217
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /inference="alignment:Splign:2.1.0"
     exon            218..282
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /inference="alignment:Splign:2.1.0"
     exon            283..414
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /inference="alignment:Splign:2.1.0"
     exon            415..478
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /inference="alignment:Splign:2.1.0"
     exon            479..605
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /inference="alignment:Splign:2.1.0"
     exon            606..775
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /inference="alignment:Splign:2.1.0"
     exon            776..843
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /inference="alignment:Splign:2.1.0"
     exon            844..911
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /inference="alignment:Splign:2.1.0"
     exon            912..994
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /inference="alignment:Splign:2.1.0"
     exon            995..1082
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /inference="alignment:Splign:2.1.0"
     exon            1083..1173
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /inference="alignment:Splign:2.1.0"
     exon            1174..1240
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /inference="alignment:Splign:2.1.0"
     exon            1241..1321
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /inference="alignment:Splign:2.1.0"
     exon            1322..1422
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /inference="alignment:Splign:2.1.0"
     exon            1423..1588
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /inference="alignment:Splign:2.1.0"
     exon            1589..1690
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /inference="alignment:Splign:2.1.0"
     exon            1691..1737
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /inference="alignment:Splign:2.1.0"
     exon            1738..1873
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /inference="alignment:Splign:2.1.0"
     exon            1874..2073
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /inference="alignment:Splign:2.1.0"
     exon            2074..2200
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /inference="alignment:Splign:2.1.0"
     exon            2201..2365
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /inference="alignment:Splign:2.1.0"
     exon            2366..2442
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /inference="alignment:Splign:2.1.0"
     exon            2443..2521
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /inference="alignment:Splign:2.1.0"
     exon            2522..2631
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /inference="alignment:Splign:2.1.0"
     exon            2632..2719
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /inference="alignment:Splign:2.1.0"
     exon            2720..2812
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /inference="alignment:Splign:2.1.0"
     exon            2813..2919
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /inference="alignment:Splign:2.1.0"
     exon            2920..3149
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /inference="alignment:Splign:2.1.0"
     exon            3150..4158
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /inference="alignment:Splign:2.1.0"
     exon            4159..4230
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /inference="alignment:Splign:2.1.0"
     exon            4231..4408
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /inference="alignment:Splign:2.1.0"
     exon            4409..4493
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /inference="alignment:Splign:2.1.0"
     exon            4494..4727
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /inference="alignment:Splign:2.1.0"
     exon            4728..4883
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /inference="alignment:Splign:2.1.0"
     exon            4884..5007
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /inference="alignment:Splign:2.1.0"
     exon            5008..5146
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /inference="alignment:Splign:2.1.0"
     exon            5147..9605
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9580..9585
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /note="hexamer: AATAAA"
     polyA_site      9605
                     /gene="RICTOR"
                     /gene_synonym="AVO3; hAVO3; PIA"
                     /note="major polyA site"
ORIGIN      
        1 gttgtgactg aaacccgtca atatggcggc gatcggccgc ggccgctctc tgaagaacct
       61 ccgagtacga gggcggaatg acagcggcga ggagaacgtc ccgctggatc tgacccgaga
      121 accttctgat aacttaagag agattctcca aaatgtggcc agattgcagg gagtatcaaa
      181 tatgagaaag ctaggccatc tgaataactt tactaagctt ctttgtgata ttggccacag
      241 tgaagaaaaa ctgggctttc actatgagga tatcataatt tgtttgcggt tagctttatt
      301 aaatgaagca aaagaagtgc gagcagcagg gctacgagcg cttcgatatc tcatccaaga
      361 ctccagtatt ctccagaagg tgctaaaatt gaaagtggac tatttaatag ctaggtgcat
      421 tgacatacaa cagagcaacg aggtagagag gacacaagca cttcgattag tcagaaagat
      481 gattactgtg aatgcttcct tgtttcctag ttctgtgacc aactcattaa ttgcagttgg
      541 aaatgatgga cttcaagaaa gagacagaat ggtccgagca tgcattgcca ttatctgtga
      601 actagcactt cagaatccag aggtggtggc ccttcgaggt ggactaaaca ccatcttgaa
      661 aaatgtgatc gattgccaat taagtcgaat aaatgaggcc ctaattacta caattttgca
      721 ccttcttaat catccaaaga ctcgacagta tgtgcgagct gatgtagaat tagagagaat
      781 tttagcaccc tatactgatt ttcactacag acatagtcca gatacagctg aaggacagct
      841 caaagaagac agagaagcac gatttctagc cagtaaaatg ggaatcatag caacattccg
      901 atcatgggca ggtattatta atttatgtaa acctggaaat tctgggatcc agtctctaat
      961 aggagtactt tgcataccaa atatggaaat aaggcgaggt ctacttgaag tgctttatga
     1021 tatatttcgt cttcctctac ctgttgtgac tgaggagttc atagaagcac tactcagtgt
     1081 agatccaggg aggttccaag acagttggag gctttcagat ggctttgtgg cagctgaggc
     1141 aaaaactatt cttcctcatc gtgccagatc caggccagac ctcatggata attatttggc
     1201 actgatactc tctgcattta ttcgtaatgg acttttagag ggtctagttg aagtgataac
     1261 aaacagtgat gatcatatct cagttagagc taccatcctt ttaggagagc ttttacatat
     1321 ggcaaacaca attcttcctc attcacatag ccatcattta cactgcttgc caaccctaat
     1381 gaatatggct gcatcctttg atatccccaa ggaaaagaga ctgcgagcca gtgcagcctt
     1441 gaactgttta aaacgcttcc atgaaatgaa gaaacgagga cctaagcctt atagtcttca
     1501 tttagaccac attattcaga aagcaattgc aacacaccag aaacgggatc agtatctccg
     1561 agttcagaaa gatatattta tccttaagga tacagaggaa gctcttttaa ttaaccttag
     1621 agatagccaa gtccttcaac ataaagagaa tcttgaatgg aattggaatc ttatagggac
     1681 cattcttaag tggccaaatg taaatctaag aaactataaa gatgaacagt tacacaggtt
     1741 tgtacgaaga ctactttatt tttacaagcc cagcagtaaa ttatatgcca acctggatct
     1801 ggattttgcc aaggccaaac agctcacggt tgtaggttgc cagtttacag aatttcttct
     1861 tgaatctgaa gaggatgggc aaggctactt agaagatcta gtaaaggata ttgttcagtg
     1921 gctcaatgct tcatctggaa tgaaacccga aagaagtctt caaaataatg gtttattgac
     1981 cacccttagt caacactact ttttatttat tggaacactt tcttgccacc ctcatggagt
     2041 taaaatgctg gaaaaatgca gtgtatttca gtgtctcctt aatctttgct ccttgaaaaa
     2101 ccaagatcac ttgctaaaac ttactgtttc tagcttggac tatagcagag atggattggc
     2161 tagagtcatc ctttccaaaa ttttaactgc agctactgat gcctgcagac tctatgcaac
     2221 aaaacattta agggtattat tgagagctaa tgttgaattc tttaataatt ggggaattga
     2281 gttgttagtg acccagctac atgataaaaa caaaacgatt tcctctgaag ctcttgatat
     2341 cctcgatgaa gcatgtgaag acaaggccaa tcttcatgct ctcattcaga tgaaaccagc
     2401 gttatcccac cttggagaca agggtttgct tctcctgctg agatttctct ccattccaaa
     2461 aggattttcc tatctgaatg aaagaggtta tgtagcaaaa caattggaaa agtggcacag
     2521 ggaatacaac tccaaatatg ttgacttgat tgaggaacaa ctcaatgaag cacttactac
     2581 ttaccggaag cctgttgatg gtgataacta tgttcgtcgg agtaaccaaa gattacagcg
     2641 tcctcacgtc tacctgccta tacaccttta tggacaacta gtacaccata aaacaggctg
     2701 ccatttgttg gaagtacaga atattattac agaactctgt cgtaatgttc gtacaccaga
     2761 tttggataag tgggaagaaa ttaaaaaact gaaagcatct ctttgggcct tgggaaatat
     2821 cggctcatca aattggggtc tcaatttgct acaggaagaa aacgtgattc cagatatact
     2881 aaaacttgca aaacagtgtg aagttctttc catcagaggg acctgtgtat atgtacttgg
     2941 gctcatagct aaaaccaaac aaggctgtga tattctaaaa tgtcacaact gggatgctgt
     3001 gaggcatagt cgcaaacatc tgtggccagt ggttccagat gatgtggaac aactctgtaa
     3061 tgaactttca tctatcccaa gcactctaag tttgaactcg gagtcaacca gctctagaca
     3121 taatagtgaa agtgaatctg tgccatcgag tatgttcata ttggaggatg accggtttgg
     3181 cagcagctct actagcacat ttttccttga tatcaatgaa gatacagagc caacatttta
     3241 tgaccgatct ggacccataa aggataaaaa ttcattccct ttctttgctt ctagtaaact
     3301 tgtgaagaat cgtatcttaa attcgcttac tttgcctaac aaaaaacatc gtagtagcag
     3361 tgatccaaaa ggagggaaat tatcatctga aagtaagaca agcaacaggc gaatcagaac
     3421 acttacggag cccagtgttg attttaatca tagtgatgat tttacaccca tatccactgt
     3481 acagaaaaca ttacaattag agacttcatt tatggggaat aagcacattg aagacactgg
     3541 tagtacacca agcattggag aaaatgactt aaaattcacc aagaattttg gtacagagaa
     3601 tcacagagaa aatacaagcc gagagaggtt agtagtagaa agttcaacga gctcacatat
     3661 gaagatacgt agccaaagtt tcaatacaga cactacaaca agtggcataa gttcaatgag
     3721 ctcaagtcct tcacgagaga cagtaggtgt agatgctaca actatggaca cagactgtgg
     3781 aagcatgagt actgtggtaa gtactaaaac tattaagaca agccactatt tgacgccaca
     3841 gtctaaccat ctgtctctct ccaaatcaaa ttcggtgtcc ctggtgcctc caggttcttc
     3901 tcatacgctt cctagaagag cacagtccct taaagcaccc tctattgcta caattaaaag
     3961 tctagcagat tgtaacttta gttacacaag ttctagagat gcttttggct atgctacact
     4021 gaaaagacta cagcaacaaa gaatgcatcc atccttatct cactctgaag ctttggcatc
     4081 tccagcaaaa gatgtgctat ttactgatac catcaccatg aaggccaaca gttttgagtc
     4141 cagattaaca ccaagcagga tcgattttaa aaagaagcat gtcgggggaa tcaggagctt
     4201 aagacctaca ataacaaaca accttttcag gttcatgaaa gccttaagtt atgcatcatt
     4261 agataaagaa gatttattga gtcctattaa tcaaaatacc ctgcaacgat cttcctcagt
     4321 gcggtccatg gtgtccagtg ccacatatgg gggttcagat gattacattg gtcttgctct
     4381 cccggtggat ataaatgata tattccaggt aaaggatatt ccctattttc agacaaaaaa
     4441 cataccacca catgatgatc gaggtgcaag agcatttgcc catgatgcag gaggtcttcc
     4501 atctggaact ggaggtcttg taaaaaattc ttttcacttg ctacgacagc agatgagtct
     4561 tacggaaata atgaattcaa tccattcaga tgcctctctg tttttagaaa gtacagaaga
     4621 cactggacta caggaacata cagatgataa ctgcctttat tgtgtctgta ttgaaattct
     4681 gggtttccag cccagcaacc aactgagtgc aatatgtagt cattcagact ttcaagatat
     4741 tccatattct gattggtgtg agcagactat ccataatcct ttagaagtgg ttccctctaa
     4801 gttttcgggg atttctggat gcagtgatgg ggtgtctcaa gaaggctcag ctagcagcac
     4861 caaaagcaca gaattgttac taggtgttaa aacaattcca gatgatacac caatgtgccg
     4921 tatactcctt cgcaaagaag ttctaagatt agtcattaat ttgagtagtt cagtttcaac
     4981 taaatgtcat gagactgggc ttttaacaat taaggagaag tatcctcaaa catttgatga
     5041 catatgcctt tactctgagg tttcccattt gctgtcacac tgcacattca gacttccgtg
     5101 tcggaggttc atacaagaat tatttcaaga tgtacagttt ctacaaatgc atgaagaagc
     5161 agaggctgtg ttggcaacac caccaaagca acctatagtt gatacatctg ctgaatcctg
     5221 acctcatatt tatgatggat atagatacat actatatata ttcatatttg tggatttcct
     5281 aaaagcctca gaaaatacga ctgactaggc agcaaagaca ggagtatctt ctgtacactg
     5341 ttccgcagtt actggtacat gaacagttgg aactgctgac tttcctaacc aaaacaactt
     5401 ccttctctcc tttgttgagc cttttgaggg gttcatgatt cattaccaca gttttaagag
     5461 tttcagttac cattgtatgc aagagccaag cactgaatac ctacataggt tttctatttt
     5521 ctttcatttt aaaagcataa tgacagtgga acaataatgg gatatgcaga agcacccttc
     5581 acaagttatt tctgaatgat ttttagggta aataatacag atgccttgtt tgttaactaa
     5641 cttgtggaaa gcaggaatca gtgtctctaa ggctgcatcc tattaccaca atggggtgtg
     5701 ctataactgc tggtattaga gagggaactt tggccctttc acgtttttct taatgtttgt
     5761 aacactactt cagaggttta taacctcaaa gcagaagaag agcctcaaca acccgggact
     5821 tataagttat ttttatgtta ctagacttgc ataaagattc ttgttttcca actcttcatt
     5881 ttgttgcaat gtgttattac aggatatatg aaccaattaa ggtttttcac tacagttctt
     5941 gaataaaatt taaaaatcat tttttatttt aattaaaaat atttcccatt tatagaatgc
     6001 atatatttgc aatggacttc cactttcatc aactttccat ctcatcgctt taaacaggaa
     6061 cttgaacaag cactgttagt ttagacctaa aggataggaa agcattaaat aatactttgg
     6121 atctcctgag gaaaagataa gtttgcttgc aatttacaca ttccatgggg aaagaagagc
     6181 catatttcct taaaaaaaac attaataaag cttgttattg agaaaaattg tagtgaaaag
     6241 ccttaagtac caaattttaa agcagcagta acttaatttt tatatcagtg tttttgtttt
     6301 gcacaaacta aatgcagtgg taggtgggtt tatgagtata ttaattgcct ttatccattt
     6361 gtgaagttaa gttgatgagg gcaaggtttt tgtttgttta atttgtatat gtctaaaggt
     6421 atttggaact ttttacagga attaaacata tatgcaaatt tgtatataaa aatagcatgg
     6481 ccatcatttg aatgcttgta aatgaaagga ttatcttttt tgagatctat atataaatag
     6541 aaatagaaaa tccagctgga ctgattagga ttctttttta attcatttgt gtataacatt
     6601 tttattacaa ttacacatca gttttgacac agtcatagca acattaatat tttcccatga
     6661 tgcagatcct ttttgtaatg ggcttgttct ttgagatctc tgtaaagaac cctgtgaact
     6721 agaaaacata actcacagag atactttttt aaaaaattta tttactggaa ctgaaagttc
     6781 cagttgggat gaagcatttc atctcacttc ataacacctc tttgactgca cttcagtgaa
     6841 ttgttcttat gtgcactgtg tagcaactta cattataaca aagcagataa gggctgtaag
     6901 ctgctgctta tgttgaaaag tggttcttca gattttctct cataaaatcc agttgaagat
     6961 aaataatttt tttatacttt atcactgaac ccaagtgttt atttaaatgt caacagtact
     7021 tctaagaacg ttgcctgtca tcgtggtctt tggtcttgga taactaaact gcctttccag
     7081 agaaccaaat gtcagagtta ctagaccaaa tagtggttaa aacctccaaa ggaagtaatg
     7141 taatcttatt cataatggga ttaacatatt ttagacattc attttaaaca ctacctcagt
     7201 taatatagag tataaaaatc tgtggtttaa tccctcaaaa gttaacagta attttttttt
     7261 tgtcttacac acacacacac cccctccccc accatcacta tccctgtacc ctcaccttgg
     7321 tcatctatcc tgaaataagg cttagttagt attggcctga atgttttgtg tttttttttt
     7381 tgtttttttt ttttactgtt actttgaaaa atatgtatgt ataccttatc atatctgcct
     7441 atatcactta ctttggggag atactcagag ctttgtggtt atcagtatac taaaaaaaaa
     7501 aaaaagtcta cgcttaaatt tatagtgcta tttggtttct ccatgatttc actgacaggt
     7561 ctaatacatt ttctttgagt acttgtttgt aaaaagtaga ctttatggtg aaaaatacat
     7621 gcagtgccaa gtgattaact taagtgttta aaaatattaa attatagcag aagaggttag
     7681 gaatgatatc agcagtaata gaaataattg agaaaatcat ctataaataa tagatattac
     7741 agactataga ataccaaaat aatgtcaata ctgtagtttt taaagatttt aggattaatc
     7801 ttagtccata taaatttgta ctattggtaa ttattgaata attgggagga atctgggcag
     7861 ttgtgctggt tgtaaactat gaatttctaa tcgtaaagtg aattgttatt tctaattgaa
     7921 ctttttttca agaacagatt tcagcctcac atactaagta aatactgata aataaggaaa
     7981 ttagaaattt agtattcata attaaatatg ctctaaaatt tcctatactt ttatttcctg
     8041 tttattctta ggtagattgg aagggggaaa cagtctgttc tccctaatta aattttttct
     8101 aataacgatt agtagaatat ggacattcta tatgacagtg acattaaaag aggctctttg
     8161 gaagtatata cattattaac ataatgtgta caagtccttt tgaaatgaca actttaatgg
     8221 gtttcagctc ttttatctag agcttgagat aattcaagct gagtttttca gggcatatca
     8281 caacggccaa gtgttcagca gtgggatatc aatgcttatt tacattttcc tactgctatt
     8341 tatataaaat gttattccat tcagaggatg ccttttatcc ccacattaaa gcacagatca
     8401 ttaagcaata aaaaccaaat tgtctgtcat tcaaattata actgcagtta tttttgcatg
     8461 gtaagagtga ggtgctaatt ttgtgtgaga tgaactttgt aaactacttt gggaaatgtt
     8521 ctttggaagt aaggtttttt ctcctttagt cttatgcttc cacttttgtc tcagattcac
     8581 aatccattaa aacatgggga aaaaagaaaa ggtaaaattg agagactttt gttagaggag
     8641 ctatttggaa tgaaccaaca tttcagattt tccaaaatgt aagttaggaa gtctccattg
     8701 tctctgcatt aacaaaatac actgttacta tcttaatctc aagagtgtca ttacagtgag
     8761 aatctcattt aaaagcatac cagtgaaatt aatagcagtg cttatcaaag aacactgaaa
     8821 tctgtgagaa tctttctagg agcattcttt tcttctttta gttccaagtt ccagggtatt
     8881 tttcattcct agtaggttta tatgactcac agaatgtgga cttttttcct gtttggagta
     8941 tttttgtaat gtaagtatcg gatagctgca ccacagcatg cataaattgc acattttgtt
     9001 ttactttctt tatagaatat ttaatttcaa aaatataatt tatgccaaaa aaagcatacc
     9061 tttcaatttt gctacttggt tgatttagca caaaatgcaa agtcttgggg cagagagggg
     9121 gagtgaaaaa aattttatag gtaattgtta caaaaatacc tgtcagaaac cctaaagctg
     9181 cattgtaaaa caaatggtgt aaactagttt tgaaaagtgg taaggaattg tgaaaaaaat
     9241 ctcagactta atgctctcta accacatgag tttcttcttt tttatttagt aatacgctgc
     9301 tacatatttg gaggttctgg tgtttgtagg tcactgaaca gacattgaaa tctgatttat
     9361 attgtataac tgtaacatag aaagaaaaag tatttatatt ttttctgtaa gaatatttca
     9421 ttgagttgtg tataatttaa ataagatttg tccccaaatg gttttgctca ccttgatttt
     9481 ttttgttgtg attttcttgt ttttgtataa tgtgtatagt ttatgtcaag ggcattaaaa
     9541 gcctcctgaa gcataatctt atcaaaggga tacattgtta ataaaatgta cttaaaattc
     9601 ttaaa
//
