LOCUS       NM_001330145            5048 bp    mRNA    linear   PRI 15-DEC-2024
DEFINITION  Homo sapiens RIC8 guanine nucleotide exchange factor B (RIC8B),
            transcript variant 1, mRNA.
ACCESSION   NM_001330145 XM_005268998
VERSION     NM_001330145.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5048)
  AUTHORS   Maureira,A., Sanchez,R., Valenzuela,N., Torrejon,M., Hinrichs,M.V.,
            Olate,J. and Gutierrez,J.L.
  TITLE     The CREB Transcription Factor Controls Transcriptional Activity of
            the Human RIC8B Gene
  JOURNAL   J Cell Biochem 117 (8), 1797-1805 (2016)
   PUBMED   26729411
  REMARK    GeneRIF: these data show the existence of functional CREB and C/EBP
            binding sites in the human RIC8B gene promoter, a particular
            distribution of these sites and demonstrate a relevant role of CREB
            in stimulating transcriptional activity of this gene.
REFERENCE   2  (bases 1 to 5048)
  AUTHORS   Lopez-Benito,S., Lillo,C., Hernandez-Hernandez,A., Chao,M.V. and
            Arevalo,J.C.
  TITLE     ARMS/Kidins220 and synembryn-B levels regulate NGF-mediated
            secretion
  JOURNAL   J Cell Sci 129 (9), 1866-1877 (2016)
   PUBMED   26966186
  REMARK    GeneRIF: overexpression of ARMS blocked NGF-mediated secretion,
            without affecting basal secretion, a decrease in ARMS resulted in
            potentiation. Similar effects were observed with synembryn-B, a
            protein that interacts directly with ARMS. Downstream of ARMS and
            synembryn-B are Galphaq and Trio proteins, which modulate the
            activity of Rac1 in response to NGF
REFERENCE   3  (bases 1 to 5048)
  AUTHORS   Jones,R.M., Cadby,G., Melton,P.E., Abraham,L.J., Whitehouse,A.J.
            and Moses,E.K.
  TITLE     Genome-wide association study of autistic-like traits in a general
            population study of young adults
  JOURNAL   Front Hum Neurosci 7, 658 (2013)
   PUBMED   24133439
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5048)
  AUTHORS   Nagai,Y., Nishimura,A., Tago,K., Mizuno,N. and Itoh,H.
  TITLE     Ric-8B stabilizes the alpha subunit of stimulatory G protein by
            inhibiting its ubiquitination
  JOURNAL   J Biol Chem 285 (15), 11114-11120 (2010)
   PUBMED   20133939
  REMARK    GeneRIF: Ric-8B plays a critical and specific role in the control
            of G alpha(s) protein levels by modulating G alpha(s)
            ubiquitination and positively regulates G(s) signaling
REFERENCE   5  (bases 1 to 5048)
  AUTHORS   Klattenhoff,C., Montecino,M., Soto,X., Guzman,L., Romo,X.,
            Garcia,M.A., Mellstrom,B., Naranjo,J.R., Hinrichs,M.V. and Olate,J.
  TITLE     Human brain synembryn interacts with Gsalpha and Gqalpha and is
            translocated to the plasma membrane in response to isoproterenol
            and carbachol
  JOURNAL   J Cell Physiol 195 (2), 151-157 (2003)
   PUBMED   12652642
  REMARK    GeneRIF: We identified a protein member of the synembryn family as
            one of the interacting proteins in human brain. Gqalpha also
            interacts with synembryn. Synembryn translocates to the plasma
            membrane in response to carbachol and isoproterenol.
REFERENCE   6  (bases 1 to 5048)
  AUTHORS   Tall,G.G., Krumins,A.M. and Gilman,A.G.
  TITLE     Mammalian Ric-8A (synembryn) is a heterotrimeric Galpha protein
            guanine nucleotide exchange factor
  JOURNAL   J Biol Chem 278 (10), 8356-8362 (2003)
   PUBMED   12509430
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA325821.1, BC034220.1, AW896637.1, AC007695.12 and BX105894.1.
            
            On Nov 22, 2018 this sequence version replaced NM_001330145.1.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.52322.1,
                                           SRR1803614.284223.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000392837.9/ ENSP00000376582.4
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-196               DA325821.1         62-257
            197-1408            BC034220.1         301-1512
            1409-1730           AW896637.1         49-370              c
            1731-3016           BC034220.1         1922-3207
            3017-4577           AC007695.12        40782-42342         c
            4578-5048           BX105894.1         13-483              c
FEATURES             Location/Qualifiers
     source          1..5048
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q23.3"
     gene            1..5048
                     /gene="RIC8B"
                     /gene_synonym="hSyn; RIC8"
                     /note="RIC8 guanine nucleotide exchange factor B"
                     /db_xref="GeneID:55188"
                     /db_xref="HGNC:HGNC:25555"
                     /db_xref="MIM:609147"
     exon            1..148
                     /gene="RIC8B"
                     /gene_synonym="hSyn; RIC8"
                     /inference="alignment:Splign:2.1.0"
     CDS             65..1747
                     /gene="RIC8B"
                     /gene_synonym="hSyn; RIC8"
                     /note="isoform 1 is encoded by transcript variant 1; brain
                     synembryn; synembryn-B; brain synembrin; resistance to
                     inhibitors of cholinesterase 8 homolog B; chaperone
                     Ric-8B"
                     /codon_start=1
                     /product="chaperone Ric-8B isoform 1"
                     /protein_id="NP_001317074.1"
                     /db_xref="CCDS:CCDS81734.1"
                     /db_xref="GeneID:55188"
                     /db_xref="HGNC:HGNC:25555"
                     /db_xref="MIM:609147"
                     /translation="MDEERALYIVRAGEAGAIERVLRDYSDKHRATFKFESTDEDKRK
                     KLCEGIFKVLIKDIPTTCQVSCLEVLRILSRDKKVLVPVTTKENMQILLRLAKLNELD
                     DSLEKVSEFPVIVESLKCLCNIVFNSQMAQQLSLELNLAAKLCNLLRKCKDRKFINDI
                     KCFDLRLLFLLSLLHTDIRSQLRYELQGLPLLTQILESAFSIKWTDEYESAIDHNGPP
                     LSPQETDCAIEALKALFNVTVDSWKVHKESDSHQFRVMAAVLRHCLLIVGPTEDKTEE
                     LHSNAVNLLSNVPVSCLDVLICPLTHEETAQEATTLDELPSNKTAEKETVLKNNTMVY
                     NGMNMEAIHVLLNFMEKRIDKGSSYREGLTPVLSLLTECSRAHRNIRKFLKDQVLPPL
                     RDVTNRPEVGSTVRNKLVRLMTHVDLGVKQIAAEFLFVLCKERVDSLLKYTGYGNAAG
                     LLAARGLLAGGRGDNWYSEDEDTDTEEYKNAKPNINLITGHLEEPMPNPIDEMTEEQK
                     EYEAMKLVNMLDKLSREELLKPMGLKPDGTITPLEEALNQYSVIEETSSDTD"
     exon            149..196
                     /gene="RIC8B"
                     /gene_synonym="hSyn; RIC8"
                     /inference="alignment:Splign:2.1.0"
     exon            197..805
                     /gene="RIC8B"
                     /gene_synonym="hSyn; RIC8"
                     /inference="alignment:Splign:2.1.0"
     exon            806..900
                     /gene="RIC8B"
                     /gene_synonym="hSyn; RIC8"
                     /inference="alignment:Splign:2.1.0"
     exon            901..1129
                     /gene="RIC8B"
                     /gene_synonym="hSyn; RIC8"
                     /inference="alignment:Splign:2.1.0"
     exon            1130..1225
                     /gene="RIC8B"
                     /gene_synonym="hSyn; RIC8"
                     /inference="alignment:Splign:2.1.0"
     exon            1226..1370
                     /gene="RIC8B"
                     /gene_synonym="hSyn; RIC8"
                     /inference="alignment:Splign:2.1.0"
     exon            1371..1515
                     /gene="RIC8B"
                     /gene_synonym="hSyn; RIC8"
                     /inference="alignment:Splign:2.1.0"
     exon            1516..1635
                     /gene="RIC8B"
                     /gene_synonym="hSyn; RIC8"
                     /inference="alignment:Splign:2.1.0"
     exon            1636..5048
                     /gene="RIC8B"
                     /gene_synonym="hSyn; RIC8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2993..2998
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RIC8B"
                     /gene_synonym="hSyn; RIC8"
                     /note="hexamer: AATAAA"
     polyA_site      3016
                     /gene="RIC8B"
                     /gene_synonym="hSyn; RIC8"
                     /note="major polyA site"
     regulatory      5026..5031
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RIC8B"
                     /gene_synonym="hSyn; RIC8"
                     /note="hexamer: AATAAA"
     polyA_site      5048
                     /gene="RIC8B"
                     /gene_synonym="hSyn; RIC8"
ORIGIN      
        1 gcgtttacct ggaggcagcg gcttgggcgc gcagagcggc cgcggctccc ccgcacctgc
       61 ggccatggat gaggagcgcg ccctctacat cgtccgggcc ggcgaagcag gggctatcga
      121 gcgggtcctg agggattaca gcgacaagca tagggctact ttcaaatttg aatcaacaga
      181 tgaagataaa agaaagaaac tctgtgaagg catatttaaa gtccttataa aggacatccc
      241 aacaacatgt caagtgtcct gcctggaagt actccgcatt ctctccagag acaaaaaggt
      301 tttagttcct gtgacaacta aggaaaatat gcagatactg ctgcgactag ccaagctaaa
      361 tgagttagat gattctttgg agaaagtatc agagttccca gttattgtgg agtcattaaa
      421 atgtctgtgt aatatagtgt tcaacagtca gatggcacag cagctcagcc tggaacttaa
      481 tcttgctgca aagctctgta acctcctgag aaagtgcaag gaccggaaat ttatcaatga
      541 cattaagtgc tttgacttgc gcttgctctt ccttctgtca cttttgcaca ccgacatcag
      601 gtcacaattg cgctatgagc tccagggact accgctgcta acgcagatct tggaaagtgc
      661 ctttagcatc aagtggaccg atgagtatga atcggccata gaccataatg gacctcctct
      721 ctcacctcag gagacagact gtgccattga ggccctcaaa gctctcttca atgtgacggt
      781 agacagttgg aaggtgcata aagagagtga ttctcatcag ttccgtgtaa tggcagctgt
      841 ccttcgtcat tgtttactaa tcgtaggtcc aactgaagac aaaacagaag agctacacag
      901 caatgcagtc aaccttttaa gcaatgttcc agtctcttgt ttggatgttc tcatttgtcc
      961 gttaacccat gaagaaacag cccaagaggc aacgactcta gatgaactgc ccagtaataa
     1021 aacagctgag aaagaaacag ttttgaaaaa caataccatg gtatacaatg gtatgaatat
     1081 ggaggccatt catgttttac tgaattttat ggagaagaga atagacaagg gaagcagcta
     1141 tagagagggt ctaactccag ttctcagctt attaaccgaa tgttcccgag cccatcgaaa
     1201 catccgaaaa tttctcaaag atcaggtttt accaccgttg agggatgtga caaatcgacc
     1261 tgaagttggc tcaactgtga gaaataagct ggtgcgcctc atgacacatg ttgaccttgg
     1321 agtcaagcaa attgctgctg aattcctttt tgtcctttgc aaagagagag tggatagtct
     1381 gctgaaatac actggctatg ggaatgctgc aggactgttg gcggccaggg gcctcttggc
     1441 tggaggaaga ggagataatt ggtactcaga ggatgaggac acagacactg aagaatacaa
     1501 aaatgcaaaa ccaaacatta atcttatcac tggtcattta gaggaaccaa tgccaaaccc
     1561 catagatgaa atgacagaag aacaaaaaga atatgaagcc atgaaacttg tcaacatgct
     1621 tgataaactt tccagagagg agttgcttaa accaatggga ctaaaacctg atgggacaat
     1681 aacgcctttg gaggaagcac tcaaccagta ctctgtcatc gaagagacca gctctgacac
     1741 agactaaaag catcacctgc tcaactctca atattgcttt atcagcatct tttctctgta
     1801 gctccagggg aatcttttct ctaaaacatt tatgcccttg ctttggctag aaacacatta
     1861 actggtttac tcattgagaa tccagcatat ttaagaggtg accctgtgtt ttttgtgata
     1921 ttgaggcatt catacagagc tgcagttaga cggggttacg ggggctaaaa gcagaaaaaa
     1981 aattccattt catcgggatg gaactgaagg attttattct ataaagcggc cctggttgaa
     2041 tctggcaatt ctttttgcca agatccctag cagaagattt agccatgtcc ttcccctcac
     2101 ttgtgtgagt ggccccttct gaatctctcc agcagccaga ggcacgtgag aagcagaaag
     2161 agctggtaaa taaagccttg ggcaagcgac ttcttagatc agaactcacc aaatggaagc
     2221 ctagcagctg ctccataaac ctagccccat tcttcatatc aattttgtat aaatatatag
     2281 aaacacacac acagcctcag acttacaaac tgattatact ctaaaagttt gtatgtcagt
     2341 tagctaaaac ttcagaatac atttctccct ataaagagtt ataaatgatg gtttagttct
     2401 caggcagcta caaatgccta tttattccct aatgtacctg aacactagta ccatagaact
     2461 gaaccaccat ctgtatcagc gcatggggag tgtgcattct gaggtctaac ccggggtgcc
     2521 aggaacacac acatcctcca tcccagcata gagaatgggg actccctgag taatgggagt
     2581 tctttcatgg cctctgcttg gggtggggga gtcagcagca cccatttgta catataggtc
     2641 attcatacat cacattttaa attggggact gtgtgtgctg gtgtgtgtgt gagttctacg
     2701 tttctaccat atgtgatcag tttaatagta actttattta tttaaaaaaa agaaacacaa
     2761 ttagttactg ttaaactgat aaagggtgtt tatttttact tttagaattg gtcctatgaa
     2821 gaagtagaaa gtgagtcatg cactagacag tgggcctagc tcatcagtgg ctaaagttga
     2881 aaaggggttg gtttcctgta tatatatgta tgtatataca cacgtacata cattcatata
     2941 tatacatata tacataatgt gcttaaccac tgccttttaa aactttaaat taaataaaac
     3001 attggggttg attcaatttg gccatctgtg tgtgtttctt tatagataca tgggctaaac
     3061 aatactattt cacagtttat gcttttttaa agtgctttta catccatggt cccacttcta
     3121 atagcctttt taaggacagg tagagtacat ggagggttac tgaaaaggtc atttatccag
     3181 aagttacctc tctggcaaag caaaaaaatc aggaattacg tcttaaaggc ctttcagctg
     3241 ccagaggaga tgtcacaaag tttcttgcaa cttacttatg ggagagatct tcaagaatat
     3301 cactcttaga ctctgaactg aaatttgcat acgcttccaa catgttttat tagctggctt
     3361 tgaagctcac aaaagaatgg caaagaaaga ggctgctaga ttgaaggcag gcatactgaa
     3421 agaagaaaaa ggccaactga aaactagata gtgagatgag aagaataaaa cttcaagaca
     3481 tagcaaatct gggactgtct acttaccaaa ggatactcta gttgacccag aaaatgtgtg
     3541 tacaacagta tggcccgtag actacttcct ataatggtga ccctgagttt ttttaaaaga
     3601 agaaattaaa tccagaacgc tttataaaag ggtaggaaga agacaataga tgttagaatt
     3661 tcatccaaaa tgtttgaggg agaggtgagt gacccaggtt ttaaccaaca gcaaaagaaa
     3721 tgggcaattc ataattttag attaagtgaa aagtttagaa aaaatttaag tatttttgtt
     3781 attctacaaa tgcattttta aagtattttt ataatttcta taatcaaata gctttggcat
     3841 gcatgtcagc ttacctaaat gaagatgtgt ttaatgaaat gccatcttac cagctttctt
     3901 tatgcagagt aagtgtttga acagtcctgt ttcagtagct gtgctcttct gctttcagtg
     3961 agttctgttt cattttgcat ttctctgcac tgggcactaa gctttgtttt tgaaaagaga
     4021 gtttgcgggg aagaagatga gagtgtcttc atctggcaca cagaaagaga actaatatct
     4081 gttaagtgct tactacatgc tgggcttttg atgtatgtta tctcatgtaa cctcacctta
     4141 tcgcatatga aagatcctct ccagaaagct tatgaaagtt accccacgtc acagcaagga
     4201 agagtccaac tctggtggcc tgactcccag caccactcct tccttttccc cacagcacac
     4261 ctgggcccac actgtgcctc cttagggctc tgaaatgctg ggaacagagt ggtgtgagga
     4321 gggtggtcat tctcaggcca ttttaagcaa taactatttc aacagaacta gtttaaagag
     4381 gtttttaagc agggaagtga cctgaagtgg aagtatgagt gggaaacttg catggtcagc
     4441 actggatttc caatgaagac ctggaatccg ttaggatagg ccagggacca aggctgcagg
     4501 ctggcctctc tgcccacaac tctcctgacc ttggaagcag ggtacggagg cgtgtgtcct
     4561 aaaataaagc atttgagctg ctgctacaga ggaacaatca ccagatattg tgaccctttc
     4621 agatgcccag tagtcctggg aggcaggcag ggcaggtgtt attatccctt ctttataaat
     4681 gaggacacag aatgaggcag tacaagtgac tctaaacacc cccttagtgc cacatcagtc
     4741 tcgccacctc ccaggactag gtgtcacctg tattgacctc ttcccctctg attataaaag
     4801 taatatatgt tcattgtgga aagtttggaa aatgcagaaa ataaaattaa taatttaccc
     4861 caaatcccac catcctgaga tcatcactgt taatgtttta gtgtatttcc ttcctgtatt
     4921 ttttctatgc atatataatt tattttacta caagattggg atcatgctgt atttacagat
     4981 ttgtatcctg ctttctttaa catattgtga gcattttccc atgtcaataa atattcttca
     5041 aaaagtaa
//
