LOCUS       NM_001286134            3506 bp    mRNA    linear   PRI 15-DEC-2024
DEFINITION  Homo sapiens RIC8 guanine nucleotide exchange factor A (RIC8A),
            transcript variant 2, mRNA.
ACCESSION   NM_001286134 XM_005253053
VERSION     NM_001286134.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Solis,G.P., Koval,A., Valnohova,J., Kazemzadeh,A., Savitsky,M. and
            Katanaev,V.L.
  TITLE     Neomorphic Galphao mutations gain interaction with Ric8 proteins in
            GNAO1 encephalopathies
  JOURNAL   J Clin Invest 134 (15), e172057 (2024)
   PUBMED   38874642
  REMARK    GeneRIF: Neomorphic Galphao mutations gain interaction with Ric8
            proteins in GNAO1 encephalopathies.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Shen,S., Yang,Y., Shen,P., Ma,J., Fang,B., Wang,Q., Wang,K.,
            Shi,P., Fan,S. and Fang,X.
  TITLE     circPDE4B prevents articular cartilage degeneration and promotes
            repair by acting as a scaffold for RIC8A and MID1
  JOURNAL   Ann Rheum Dis 80 (9), 1209-1219 (2021)
   PUBMED   34039624
  REMARK    GeneRIF: circPDE4B prevents articular cartilage degeneration and
            promotes repair by acting as a scaffold for RIC8A and MID1.
REFERENCE   3  (bases 1 to 3506)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 3506)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 3506)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 3506)
  AUTHORS   Yu,H., Tardivo,L., Tam,S., Weiner,E., Gebreab,F., Fan,C.,
            Svrzikapa,N., Hirozane-Kishikawa,T., Rietman,E., Yang,X.,
            Sahalie,J., Salehi-Ashtiani,K., Hao,T., Cusick,M.E., Hill,D.E.,
            Roth,F.P., Braun,P. and Vidal,M.
  TITLE     Next-generation sequencing to generate interactome datasets
  JOURNAL   Nat Methods 8 (6), 478-480 (2011)
   PUBMED   21516116
REFERENCE   7  (bases 1 to 3506)
  AUTHORS   Woodard,G.E., Huang,N.N., Cho,H., Miki,T., Tall,G.G. and Kehrl,J.H.
  TITLE     Ric-8A and Gi alpha recruit LGN, NuMA, and dynein to the cell
            cortex to help orient the mitotic spindle
  JOURNAL   Mol Cell Biol 30 (14), 3519-3530 (2010)
   PUBMED   20479129
  REMARK    GeneRIF: Ric-8A signaling leads to assembly of a cortical signaling
            complex that functions to orient the mitotic spindle.
REFERENCE   8  (bases 1 to 3506)
  AUTHORS   Nishimura,A., Okamoto,M., Sugawara,Y., Mizuno,N., Yamauchi,J. and
            Itoh,H.
  TITLE     Ric-8A potentiates Gq-mediated signal transduction by acting
            downstream of G protein-coupled receptor in intact cells
  JOURNAL   Genes Cells 11 (5), 487-498 (2006)
   PUBMED   16629901
REFERENCE   9  (bases 1 to 3506)
  AUTHORS   Tall,G.G., Krumins,A.M. and Gilman,A.G.
  TITLE     Mammalian Ric-8A (synembryn) is a heterotrimeric Galpha protein
            guanine nucleotide exchange factor
  JOURNAL   J Biol Chem 278 (10), 8356-8362 (2003)
   PUBMED   12509430
REFERENCE   10 (bases 1 to 3506)
  AUTHORS   Miller,K.G., Emerson,M.D., McManus,J.R. and Rand,J.B.
  TITLE     RIC-8 (Synembryn): a novel conserved protein that is required for
            G(q)alpha signaling in the C. elegans nervous system
  JOURNAL   Neuron 27 (2), 289-299 (2000)
   PUBMED   10985349
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC069287.7.
            
            On Sep 16, 2020 this sequence version replaced NM_001286134.1.
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site, compared to variant 1. The encoded isoform (2) is
            shorter than isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK026897.1, AL390088.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000526104.6/ ENSP00000432008.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1231              AC069287.7         50550-51780         c
            1232-1279           AC069287.7         50170-50217         c
            1280-1873           AC069287.7         49488-50081         c
            1874-1965           AC069287.7         48826-48917         c
            1966-2116           AC069287.7         48139-48289         c
            2117-2212           AC069287.7         46977-47072         c
            2213-2357           AC069287.7         46729-46873         c
            2358-2502           AC069287.7         46507-46651         c
            2503-2622           AC069287.7         46070-46189         c
            2623-3506           AC069287.7         44375-45258         c
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11p15.5"
     gene            1..3506
                     /gene="RIC8A"
                     /gene_synonym="RIC8"
                     /note="RIC8 guanine nucleotide exchange factor A"
                     /db_xref="GeneID:60626"
                     /db_xref="HGNC:HGNC:29550"
                     /db_xref="MIM:609146"
     exon            1..1231
                     /gene="RIC8A"
                     /gene_synonym="RIC8"
                     /inference="alignment:Splign:2.1.0"
     CDS             1148..2743
                     /gene="RIC8A"
                     /gene_synonym="RIC8"
                     /note="isoform 2 is encoded by transcript variant 2;
                     synembryn-A; resistance to inhibitors of cholinesterase 8
                     homolog A; chaperone Ric-8A"
                     /codon_start=1
                     /product="chaperone Ric-8A isoform 2"
                     /protein_id="NP_001273063.1"
                     /db_xref="CCDS:CCDS65982.1"
                     /db_xref="GeneID:60626"
                     /db_xref="HGNC:HGNC:29550"
                     /db_xref="MIM:609146"
                     /translation="MEPRAVAEAVETGEEDVIMEALRSYNQEHSQSFTFDDAQQEDRK
                     RLAELLVSVLEQGLPPSHRVIWLQSVRILSRDRNCLDPFTSRQSLQALACYADISVSE
                     GSVPESADMDVVLESLKCLCNLVLSSPVAQMLAAEARLVVKLTERVGLYRERSFPHDV
                     QFFDLRLLFLLTALRTDVRQQLFQELKGVRLLTDTLELTLGVTPEGNPPPTLLPSQET
                     ERAMEILKVLFNITLDSIKGEVDEEDAALYRHLGTLLRHCVMIATAGDRTEEFHGHAV
                     NLLGNLPLKCLDVLLTLEPHGDSTEFMGVNMDVIRALLIFLEKRLHKTHRLKESVAPV
                     LSVLTECARMHRPARKFLKAQVLPPLRDVRTRPEVGEMLRNKLVRLMTHLDTDVKRVA
                     AEFLFVLCSESVPRFIKYTGYGNAAGLLAARGLMAGGRPEGQYSEDEDTDTDEYKEAK
                     ASINPVTGRVEEKPPNPMEGMTEEQKEHEAMKLVTMFDKLSRNRVIQPMGMSPRGHLT
                     SLQDAMCETMEQQLSSDPDSDPD"
     exon            1232..1279
                     /gene="RIC8A"
                     /gene_synonym="RIC8"
                     /inference="alignment:Splign:2.1.0"
     exon            1280..1873
                     /gene="RIC8A"
                     /gene_synonym="RIC8"
                     /inference="alignment:Splign:2.1.0"
     exon            1874..1965
                     /gene="RIC8A"
                     /gene_synonym="RIC8"
                     /inference="alignment:Splign:2.1.0"
     exon            1966..2116
                     /gene="RIC8A"
                     /gene_synonym="RIC8"
                     /inference="alignment:Splign:2.1.0"
     exon            2117..2212
                     /gene="RIC8A"
                     /gene_synonym="RIC8"
                     /inference="alignment:Splign:2.1.0"
     exon            2213..2357
                     /gene="RIC8A"
                     /gene_synonym="RIC8"
                     /inference="alignment:Splign:2.1.0"
     exon            2358..2502
                     /gene="RIC8A"
                     /gene_synonym="RIC8"
                     /inference="alignment:Splign:2.1.0"
     exon            2503..2622
                     /gene="RIC8A"
                     /gene_synonym="RIC8"
                     /inference="alignment:Splign:2.1.0"
     exon            2623..3506
                     /gene="RIC8A"
                     /gene_synonym="RIC8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3482..3487
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RIC8A"
                     /gene_synonym="RIC8"
                     /note="hexamer: AATAAA"
     polyA_site      3506
                     /gene="RIC8A"
                     /gene_synonym="RIC8"
                     /note="major polyA site"
ORIGIN      
        1 gctcagtgcc tagacgatgg tggtggaaag acgcttctgt gggttaggtc ctaacggtta
       61 ggaaggattc acaggcgggt catgaggaag gagcagaccc ctcgggcaga agcccgcgag
      121 gagcactggg tgtggtgggg gcaggggtgc ctcctctgtc aagtggagat cggcgcctcg
      181 ctcgtgcctg cgagtgcgcg ccgcgtcgaa tacagccgta cagaacgcac tacaggaaag
      241 tgaagccggg agagagactg ggaggcggcg cggggactgg gggccgtaga cgcctttgct
      301 cccgggcact tgttagaaga gcgagtggat cgcgcttcct gtgcgtcact gaatcctcat
      361 gacggccact gaaacggaca cgcaggatct ttgcttttca agagggaact gagtccaggc
      421 cagactgggt tgctcccctt tctctcggcc cctccacctt tgcaagtgct gctccttctg
      481 cctggagtgg ctcctccagt cttttcccgg ctcacttccc accgtccggt gtcagcaaaa
      541 acagcacttc ctcacagccc gttgcagccc agcagtcgcc ttttgcttct tttgaagcac
      601 agccctgtga ccgcctcgtg tccttttgtt tttccttgtc ccccttcccc tctggaacat
      661 aagctccaca aaggcaggga cctgggaatc ccgttcgcct gcattcccca gcaaacgcct
      721 aacgaaccca atggcccagg actgaaaatt cctttccact tcggctttcc cctctacatc
      781 ccatcggttc tgtggagctc gaggggcgtc actgtgtgtg tggcagggca cggtgggggc
      841 tgagatcgtt tcctgttgga acttctggcc caagaagcgc gggtcacaag gagaggggtc
      901 agttcggttc agagcgactc agcccctcga ctcgggtctt aaaacctccg agccgccagt
      961 tctgcctcag gccgcgcccc cttaaagcgc caccagacgc tgcgccccgt taaagcgcca
     1021 ccagacgccg cgccccgtcc cggcctcccc cgcgcgctgg cgcggggctt tctgggccag
     1081 ggcggggccg gcgaactgcg gcccggaacg gctgaggaag ggcccgtccc gccttccccg
     1141 gcgcgccatg gagccccggg cggttgcaga agccgtggag acgggtgagg aggatgtgat
     1201 tatggaagct ctgcggtcat acaaccagga gcactcccag agcttcacgt ttgatgatgc
     1261 ccaacaggag gaccggaaga gactggcgga gctgctggtc tccgtcctgg aacagggctt
     1321 gccaccctcc caccgtgtca tctggctgca gagtgtccga atcctgtccc gggaccgcaa
     1381 ctgcctggac ccgttcacca gccgccagag cctgcaggca ctagcctgct atgctgacat
     1441 ctctgtctct gaggggtccg tcccagagtc cgcagacatg gatgttgtac tggagtccct
     1501 caagtgcctg tgcaacctcg tgctcagcag ccctgtggca cagatgctgg cagcagaggc
     1561 ccgcctagtg gtgaagctca cagagcgtgt ggggctgtac cgtgagagga gcttccccca
     1621 cgatgtccag ttctttgact tgcggctcct cttcctgcta acggcactcc gcaccgatgt
     1681 gcgccagcag ctgtttcagg agctgaaagg agtgcgcctg ctaactgaca cactggagct
     1741 gacgctgggg gtgactcctg aagggaaccc cccacccacg ctccttcctt cccaagagac
     1801 tgagcgggcc atggagatcc tcaaagtgct cttcaacatc accctggact ccatcaaggg
     1861 ggaggtggac gaggaagacg ctgcccttta ccgacacctg gggacccttc tccggcactg
     1921 tgtgatgatc gctactgctg gagaccgcac agaggagttc cacggccacg cagtgaacct
     1981 cctggggaac ttgcccctca agtgtctgga tgttctcctc accctggagc cacatggaga
     2041 ctccacggag ttcatgggag tgaatatgga tgtgattcgt gccctcctca tcttcctaga
     2101 gaagcgtttg cacaagacac acaggctgaa ggagagtgta gctcccgtgc tgagcgtgct
     2161 gactgaatgt gcccggatgc accgcccagc caggaagttc ctgaaggccc aggtgctgcc
     2221 ccctctgcgg gatgtgagga cacggcctga ggttggggag atgctgcgga acaagcttgt
     2281 ccgcctcatg acacacctgg acacagatgt gaagagggtg gctgccgagt tcttgtttgt
     2341 cctgtgctct gagagtgtgc cccgattcat caagtacaca ggctatggga atgctgctgg
     2401 ccttctggct gccaggggcc tcatggcagg aggccggccc gagggccagt actcagagga
     2461 tgaggacaca gacacagatg agtacaagga agccaaagcc agcataaacc ctgtgaccgg
     2521 gagggtggag gagaagccgc ctaaccctat ggagggcatg acagaggagc agaaggagca
     2581 cgaggccatg aagctggtga ccatgtttga caagctctcc aggaacagag tcatccagcc
     2641 aatggggatg agtccccggg gtcatcttac gtccctgcag gatgccatgt gcgagactat
     2701 ggagcagcag ctctcctcgg accctgactc ggaccctgac tgaggatggc agctcttctg
     2761 ctcccccatc aggactggtg ctgcttccag agacttcctt ggggttgcaa cctggggaag
     2821 ccacatccca ctggatccac acccgccccc acttctccat cttagaaacc ccttctcttg
     2881 actcccgttc tgttcatgat ttgcctctgg tccagtttct catctctgga ctgcaacggt
     2941 cttcttgtgc tagaactcag gctcagcctc gaattccaca gacgaagtac tttcttttgt
     3001 ctgcgccaag aggaatgtgt tcagaagctg ctgcctgagg gcagggccta cctgggcaca
     3061 cagaagagca tatgggaggg caggggtttg ggtgtgggtg cacacaaagc aagcaccatc
     3121 tgggattggc acactggcag agccagtgtg ttggggtatg tgctgcactt cccagggaga
     3181 aaacctgtca gaactttcca tacgagtata tcagaacaca cccttccaag gtatgtatgc
     3241 tctgttgttc ctgtcctgtc ttcactgagc gcagggctgg aggcctctta gacattctcc
     3301 ttggtcctcg ttcagctgcc cactgtagta tccacagtgc ccgagttctc gctggttttg
     3361 gcaattaaac ctccttccta ctggtttaga ctacacttac aacaaggaaa atgcccctcg
     3421 tgtgaccata gattgagatt tataccacat accacacata gccacagaaa catcatcttg
     3481 aaataaagaa gagttttgga caaaaa
//
