LOCUS       NM_033103               3501 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens rhophilin Rho GTPase binding protein 2 (RHPN2), mRNA.
ACCESSION   NM_033103
VERSION     NM_033103.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Chen,H., Shen,D., Zhu,F., Ou,Q., Cheng,L. and Zhu,Y.
  TITLE     Long Noncoding RNA RP11-89K21.1 Interacts with miR-146a/b-5p to
            Promote Proliferation and Gefitinib Resistance Through Regulating
            RHPN2 and RhoA/ROCK Pathway in Lung Adenocarcinoma
  JOURNAL   Cancer Biother Radiopharm 38 (5), 282-292 (2023)
   PUBMED   33877886
  REMARK    GeneRIF: Long Noncoding RNA RP11-89K21.1 Interacts with
            miR-146a/b-5p to Promote Proliferation and Gefitinib Resistance
            Through Regulating RHPN2 and RhoA/ROCK Pathway in Lung
            Adenocarcinoma.
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Liu,Z., Fang,F., Li,J., Zhao,G., Zang,Q., Zhang,F. and Die,J.
  TITLE     [RHPN2 is highly expressed in osteosarcoma cells to promote cell
            proliferation and migration and inhibit apoptosis]
  JOURNAL   Nan Fang Yi Ke Da Xue Xue Bao 42 (9), 1367-1373 (2022)
   PUBMED   36210710
  REMARK    GeneRIF: [RHPN2 is highly expressed in osteosarcoma cells to
            promote cell proliferation and migration and inhibit apoptosis].
REFERENCE   3  (bases 1 to 3501)
  AUTHORS   Yan,L., He,Z., Li,W., Liu,N. and Gao,S.
  TITLE     P76RBE silencing inhibits ovarian cancer cell proliferation,
            migration, and invasion via suppressing the integrin
            beta1/NF-kappaB pathway
  JOURNAL   Cell Cycle 20 (18), 1875-1889 (2021)
   PUBMED   34382920
  REMARK    GeneRIF: P76RBE silencing inhibits ovarian cancer cell
            proliferation, migration, and invasion via suppressing the integrin
            beta1/NF-kappaB pathway.
REFERENCE   4  (bases 1 to 3501)
  AUTHORS   Jiang,S., Mo,C., Guo,S., Zhuang,J., Huang,B. and Mao,X.
  TITLE     Human bone marrow mesenchymal stem cells-derived
            microRNA-205-containing exosomes impede the progression of prostate
            cancer through suppression of RHPN2
  JOURNAL   J Exp Clin Cancer Res 38 (1), 495 (2019)
   PUBMED   31847864
  REMARK    GeneRIF: The hBMSCs-derived exosomal miR-205 retards prostate
            cancer progression by inhibiting RHPN2, suggesting that miR-205 may
            present a predictor and potential therapeutic target for prostate
            cancer.
            Erratum:[J Exp Clin Cancer Res. 2022 Jun 18;41(1):206. doi:
            10.1186/s13046-022-02417-y.. PMID: 35717208]
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3501)
  AUTHORS   Chen,C., Guo,Q., Shi,J., Jiao,X., Lv,K., Liu,X., Jiang,Y., Hui,X.
            and Song,T.
  TITLE     Genetic variants of MGMT, RHPN2, and FAM49A contributed to
            susceptibility of nonsyndromic orofacial clefts in a Chinese
            population
  JOURNAL   J Oral Pathol Med 47 (8), 796-801 (2018)
   PUBMED   29949196
  REMARK    GeneRIF: It may contribute to the pathogenesis of nonsyndromic
            orofacial clefts in Chinese populations.
REFERENCE   6  (bases 1 to 3501)
  AUTHORS   Hawken,S.J., Greenwood,C.M., Hudson,T.J., Kustra,R., McLaughlin,J.,
            Yang,Q., Zanke,B.W. and Little,J.
  TITLE     The utility and predictive value of combinations of low penetrance
            genes for screening and risk prediction of colorectal cancer
  JOURNAL   Hum Genet 128 (1), 89-101 (2010)
   PUBMED   20437058
  REMARK    GeneRIF: Observational study of genetic testing. (HuGE Navigator)
REFERENCE   7  (bases 1 to 3501)
  AUTHORS   Niittymaki,I., Kaasinen,E., Tuupanen,S., Karhu,A., Jarvinen,H.,
            Mecklin,J.P., Tomlinson,I.P., Di Bernardo,M.C., Houlston,R.S. and
            Aaltonen,L.A.
  TITLE     Low-penetrance susceptibility variants in familial colorectal
            cancer
  JOURNAL   Cancer Epidemiol Biomarkers Prev 19 (6), 1478-1483 (2010)
   PUBMED   20501757
  REMARK    GeneRIF: Observational study of gene-disease association, gene-gene
            interaction, and gene-environment interaction. (HuGE Navigator)
REFERENCE   8  (bases 1 to 3501)
  AUTHORS   Zhan,X. and Desiderio,D.M.
  TITLE     Nitroproteins from a human pituitary adenoma tissue discovered with
            a nitrotyrosine affinity column and tandem mass spectrometry
  JOURNAL   Anal Biochem 354 (2), 279-289 (2006)
   PUBMED   16777052
REFERENCE   9  (bases 1 to 3501)
  AUTHORS   Mircescu,H., Steuve,S., Savonet,V., Degraef,C., Mellor,H.,
            Dumont,J.E., Maenhaut,C. and Pirson,I.
  TITLE     Identification and characterization of a novel activated RhoB
            binding protein containing a PDZ domain whose expression is
            specifically modulated in thyroid cells by cAMP
  JOURNAL   Eur J Biochem 269 (24), 6241-6249 (2002)
   PUBMED   12473120
  REMARK    GeneRIF: may play a key role between RhoB and potential downstream
            elements needed under stimulation of the thyrotropin/cAMP pathway
            in thyrocytes
REFERENCE   10 (bases 1 to 3501)
  AUTHORS   Peck,J.W., Oberst,M., Bouker,K.B., Bowden,E. and Burbelo,P.D.
  TITLE     The RhoA-binding protein, rhophilin-2, regulates actin cytoskeleton
            organization
  JOURNAL   J Biol Chem 277 (46), 43924-43932 (2002)
   PUBMED   12221077
  REMARK    GeneRIF: Rhophilin-2 may function normally in a Rho pathway to
            limit stress fiber formation and/or increase the turnover of
            F-actin structures in the absence of high levels of RhoA activity
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC348657.1, AJ347750.1,
            AC011449.6 and BC036447.2.
            
            On Nov 23, 2018 this sequence version replaced NM_033103.4.
            
            Summary: This gene encodes a member of the rhophilin family of
            Ras-homologous (Rho)-GTPase binding proteins. The encoded protein
            binds both GTP- and GDP-bound RhoA and GTP-bound RhoB and may be
            involved in the organization of the actin cytoskeleton. [provided
            by RefSeq, Apr 2009].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC036447.2, AK098246.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000254260.8/ ENSP00000254260.2
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-36                DC348657.1         31-66
            37-1316             AJ347750.1         1-1280
            1317-1317           AC011449.6         99532-99532         c
            1318-2097           AJ347750.1         1282-2061
            2098-3501           BC036447.2         2098-3501
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.11"
     gene            1..3501
                     /gene="RHPN2"
                     /gene_synonym="P76RBE; RHOBP"
                     /note="rhophilin Rho GTPase binding protein 2"
                     /db_xref="GeneID:85415"
                     /db_xref="HGNC:HGNC:19974"
                     /db_xref="MIM:617932"
     exon            1..105
                     /gene="RHPN2"
                     /gene_synonym="P76RBE; RHOBP"
                     /inference="alignment:Splign:2.1.0"
     CDS             37..2097
                     /gene="RHPN2"
                     /gene_synonym="P76RBE; RHOBP"
                     /note="76 kDa RhoB effector protein; GTP-Rho-binding
                     protein 2; rhophilin-like Rho-GTPase binding protein"
                     /codon_start=1
                     /product="rhophilin-2"
                     /protein_id="NP_149094.3"
                     /db_xref="CCDS:CCDS12427.1"
                     /db_xref="GeneID:85415"
                     /db_xref="HGNC:HGNC:19974"
                     /db_xref="MIM:617932"
                     /translation="MTDALLPAAPQPLEKENDGYFRKGCNPLAQTGRSKLQNQRAALN
                     QQILKAVRMRTGAENLLKVATNSKVREQVRLELSFVNSDLQMLKEELEGLNISVGVYQ
                     NTEEAFTIPLIPLGLKETKDVDFAVVLKDFILEHYSEDGYLYEDEIADLMDLRQACRT
                     PSRDEAGVELLMTYFIQLGFVESRFFPPTRQMGLLFTWYDSLTGVPVSQQNLLLEKAS
                     VLFNTGALYTQIGTRCDRQTQAGLESAIDAFQRAAGVLNYLKDTFTHTPSYDMSPAML
                     SVLVKMMLAQAQESVFEKISLPGIRNEFFMLVKVAQEAAKVGEVYQQLHAAMSQAPVK
                     ENIPYSWASLACVKAHHYAALAHYFTAILLIDHQVKPGTDLDHQEKCLSQLYDHMPEG
                     LTPLATLKNDQQRRQLGKSHLRRAMAHHEESVREASLCKKLRSIEVLQKVLCAAQERS
                     RLTYAQHQEEDDLLNLIDAPSVVAKTEQEVDIILPQFSKLTVTDFFQKLGPLSVFSAN
                     KRWTPPRSIRFTAEEGDLGFTLRGNAPVQVHFLDPYCSASVAGAREGDYIVSIQLVDC
                     KWLTLSEVMKLLKSFGEDEIEMKVVSLLDSTSSMHNKSATYSVGMQKTYSMICLAIDD
                     DDKTDKTKKISKKLSFLSWGTNKNRQKSASTLCLPSVGAARPQVKKKLPSPFSLLNSD
                     SSWY"
     exon            106..221
                     /gene="RHPN2"
                     /gene_synonym="P76RBE; RHOBP"
                     /inference="alignment:Splign:2.1.0"
     exon            222..350
                     /gene="RHPN2"
                     /gene_synonym="P76RBE; RHOBP"
                     /inference="alignment:Splign:2.1.0"
     exon            351..426
                     /gene="RHPN2"
                     /gene_synonym="P76RBE; RHOBP"
                     /inference="alignment:Splign:2.1.0"
     exon            427..504
                     /gene="RHPN2"
                     /gene_synonym="P76RBE; RHOBP"
                     /inference="alignment:Splign:2.1.0"
     exon            505..629
                     /gene="RHPN2"
                     /gene_synonym="P76RBE; RHOBP"
                     /inference="alignment:Splign:2.1.0"
     exon            630..796
                     /gene="RHPN2"
                     /gene_synonym="P76RBE; RHOBP"
                     /inference="alignment:Splign:2.1.0"
     exon            797..984
                     /gene="RHPN2"
                     /gene_synonym="P76RBE; RHOBP"
                     /inference="alignment:Splign:2.1.0"
     exon            985..1141
                     /gene="RHPN2"
                     /gene_synonym="P76RBE; RHOBP"
                     /inference="alignment:Splign:2.1.0"
     exon            1142..1261
                     /gene="RHPN2"
                     /gene_synonym="P76RBE; RHOBP"
                     /inference="alignment:Splign:2.1.0"
     exon            1262..1456
                     /gene="RHPN2"
                     /gene_synonym="P76RBE; RHOBP"
                     /inference="alignment:Splign:2.1.0"
     exon            1457..1533
                     /gene="RHPN2"
                     /gene_synonym="P76RBE; RHOBP"
                     /inference="alignment:Splign:2.1.0"
     exon            1534..1680
                     /gene="RHPN2"
                     /gene_synonym="P76RBE; RHOBP"
                     /inference="alignment:Splign:2.1.0"
     exon            1681..1836
                     /gene="RHPN2"
                     /gene_synonym="P76RBE; RHOBP"
                     /inference="alignment:Splign:2.1.0"
     exon            1837..3501
                     /gene="RHPN2"
                     /gene_synonym="P76RBE; RHOBP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3479..3484
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RHPN2"
                     /gene_synonym="P76RBE; RHOBP"
                     /note="hexamer: AATAAA"
     polyA_site      3501
                     /gene="RHPN2"
                     /gene_synonym="P76RBE; RHOBP"
                     /note="major polyA site"
ORIGIN      
        1 agtccgccgt ccgccctccg cgcccgcgcc gctagcatga ccgacgcgct gttgcccgcg
       61 gccccccagc cgctggagaa ggagaacgac ggctactttc ggaagggctg taatcccctt
      121 gcacaaaccg gccggagtaa attgcagaat caaagagctg ctttgaatca gcagatcctg
      181 aaagccgtgc ggatgaggac cggagcggaa aaccttctga aagtggccac aaactcaaag
      241 gtgcgggagc aagtgcggct ggagctgagc ttcgtcaact cagacctgca gatgctcaag
      301 gaagagctgg aggggctgaa catctcggtg ggcgtctatc agaacacaga ggaggcattt
      361 acgattcccc tgattcctct tggcctgaag gaaacgaaag acgtcgactt tgcagtcgtc
      421 ctcaaggatt ttatcctgga acattacagt gaagatggct atttatatga agatgaaatt
      481 gcagatctta tggatctgag acaagcttgt cggacgccta gccgggatga ggccggggtg
      541 gaactgctga tgacatactt catccagctg ggctttgtcg agagtcgatt cttcccgccc
      601 acacggcaga tgggactcct gttcacctgg tatgactctc tcaccggggt tccggtcagc
      661 cagcagaacc tgctgctgga gaaggccagt gtcctgttca acactggggc cctctacacc
      721 cagattggga cccggtgtga tcggcagacg caggctgggc tggagagtgc catagatgcc
      781 tttcagagag ccgcaggggt tttaaattac ctgaaagaca catttaccca tactccaagt
      841 tacgacatga gccctgccat gctcagcgtg ctcgtcaaaa tgatgcttgc acaagcccaa
      901 gaaagcgtgt ttgagaaaat cagccttcct gggatccgga atgaattctt catgctggtg
      961 aaggtggctc aggaggctgc taaggtggga gaggtctacc aacagctaca cgcagccatg
     1021 agccaggcgc cggtgaaaga gaacatcccc tactcctggg ccagcttagc ctgcgtgaag
     1081 gcccaccact acgcggccct ggcccactac ttcactgcca tcctcctcat cgaccaccag
     1141 gtgaagccag gcacggatct ggaccaccag gagaagtgcc tgtcccagct ctacgaccac
     1201 atgccagagg ggctgacacc cttggccaca ctgaagaatg atcagcagcg ccgacagctg
     1261 gggaagtccc acttgcgcag agccatggct catcacgagg agtcggtgcg ggaggccagc
     1321 ctctgcaaga agctgcggag cattgaggtg ctacagaagg tgctgtgtgc cgcacaggaa
     1381 cgctcccggc tcacgtacgc ccagcaccag gaggaggatg acctgctgaa cctgatcgac
     1441 gcccccagtg ttgttgctaa aactgagcaa gaggttgaca ttatattgcc ccagttctcc
     1501 aagctgacag tcacggactt cttccagaag ctgggcccct tatctgtgtt ttcggctaac
     1561 aagcggtgga cgcctcctcg aagcatccgc ttcactgcag aagaagggga cttggggttc
     1621 accttgagag ggaacgcccc cgttcaggtt cacttcctgg atccttactg ctctgcctcg
     1681 gtggcaggag cccgggaagg agattatatt gtctccattc agcttgtgga ttgtaagtgg
     1741 ctgacgctga gtgaggttat gaagctgctg aagagctttg gcgaggacga gatcgagatg
     1801 aaagtcgtga gcctcctgga ctccacatca tccatgcata ataagagtgc cacatactcc
     1861 gtgggaatgc agaaaacgta ctccatgatc tgcttagcca ttgatgatga cgacaaaact
     1921 gataaaacca agaaaatctc caagaagctt tccttcctga gttggggcac caacaagaac
     1981 agacagaagt cagccagcac cttgtgcctc ccatcggtcg gggctgcacg gcctcaggtc
     2041 aagaagaagc tgccctcccc tttcagcctt ctcaactcag acagttcttg gtactaatgt
     2101 gaggaaacaa acatgttcag gccctgaaca tttccggtgc tgactcggcc ttaaacgttt
     2161 gtgccataat ggaaaatatc tatctatctg ttctcaaatc ctgtttttct catagtgtaa
     2221 actcacattt gatgtgtttt tatgaaggaa agtaaccaag aaacctctag gaattagtga
     2281 aaaaagaact tttttgaggt gtgttactat actgctgtaa gttatttatt atataaagta
     2341 ttgtaaatag aatagtgttg aagatatgaa atatggctat ttttaatggt gacaattatg
     2401 acttttagtc actattaaat tggggttacc tatatcagta caatttgtag ttgtttccag
     2461 gtttggctaa taatcattcc ttaacctaga attcagatga tcctggaatt aaggcaggtc
     2521 agaggactgt aatgatagaa ttaaattagt gtcactaaaa actgtcccaa agtgctgctt
     2581 cctaatagga attcattaac ctaaaacaag atgttactat tatatcgata gactatgaat
     2641 gctatttcta gaaaaagtct agtgccaaat ttgtcttatt aaataaaaac aatgtaggag
     2701 cagcttttct tctagtttga tgtcatttaa gaattactaa cacagtggca gtgttagatg
     2761 aagatgctgt ctacaaggta gataatatac tgtttgatac tcaaaacatt tttcattttg
     2821 tttaaagtag aagttacata attctatatt ttaagtcttg ggtaaaaaag tagttttaca
     2881 ttttataaag taaagatgta aatgattcag gtttaaagct ctatttgact tccttttttt
     2941 gtttgagata gcgtcttgct gtgttgccca ggctggagtg cagtggtgtg atctcagctc
     3001 agtgcaacct ccgccccctg ggatcaagcg attctcctac ctcagcctcc caaatagctg
     3061 ggactacaag gtgccctcca gcatgcctgg ctgatttttg tatttttagt tgaggtgagg
     3121 tttcaccatg ttggccaggc gggtttcgaa atcctgacct caaatgatcc acccacctca
     3181 gcctcccaaa gtgctgggat tacaggcatg agccaccaca accgtcccac tattttactt
     3241 tttaaaatga cattcctact gattgatttt tatcttgcta taagttcgat gacaccgtga
     3301 atctaataag gttcactgtt gacacagtac aagttacata gctaaaatac atagcattga
     3361 agactaattt taaggattga caagagttta ttttctattg tgcaatatct taaaggaagc
     3421 aaccaccttt gggaaagtgt atctgctgct cctagggcca tgcttgtata catatttaaa
     3481 taaacatatt catttacccg a
//
