LOCUS       NM_032498               2848 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens Rhox homeobox family member 2 (RHOXF2), mRNA.
ACCESSION   NM_032498
VERSION     NM_032498.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 2848)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2848)
  AUTHORS   Matlock,A.O., Smith,B.A. and Jackman,J.E.
  TITLE     Chemical footprinting and kinetic assays reveal dual functions for
            highly conserved eukaryotic tRNAHis guanylyltransferase residues
  JOURNAL   J Biol Chem 294 (22), 8885-8893 (2019)
   PUBMED   31000629
  REMARK    GeneRIF: Human Thg1, two critical tRNA-interacting residues,
            His-152 and Lys-187, alanine-substituted variants maintain a
            similar overall interaction with the anticodon region, arguing
            against the sufficiency of this interaction for driving catalysis.
            Instead, conservative mutagenesis revealed a new direct function
            for these residues in recognition of a non-Watson-Crick G-1:A73 bp
REFERENCE   5  (bases 1 to 2848)
  AUTHORS   Yin,Y., Morgunova,E., Jolma,A., Kaasinen,E., Sahu,B.,
            Khund-Sayeed,S., Das,P.K., Kivioja,T., Dave,K., Zhong,F.,
            Nitta,K.R., Taipale,M., Popov,A., Ginno,P.A., Domcke,S., Yan,J.,
            Schubeler,D., Vinson,C. and Taipale,J.
  TITLE     Impact of cytosine methylation on DNA binding specificities of
            human transcription factors
  JOURNAL   Science 356 (6337) (2017)
   PUBMED   28473536
REFERENCE   6  (bases 1 to 2848)
  AUTHORS   Shibata-Minoshima,F., Oki,T., Doki,N., Nakahara,F., Kageyama,S.,
            Kitaura,J., Fukuoka,J. and Kitamura,T.
  TITLE     Identification of RHOXF2 (PEPP2) as a cancer-promoting gene by
            expression cloning
  JOURNAL   Int J Oncol 40 (1), 93-98 (2012)
   PUBMED   21874235
  REMARK    GeneRIF: RHOXF2 is highly expressed in some leukemia cell lines and
            a variety of human cancers. Results indicate that RHOXF2 is
            involved in carcinogenesis.
REFERENCE   7  (bases 1 to 2848)
  AUTHORS   Ravasi,T., Suzuki,H., Cannistraci,C.V., Katayama,S., Bajic,V.B.,
            Tan,K., Akalin,A., Schmeier,S., Kanamori-Katayama,M., Bertin,N.,
            Carninci,P., Daub,C.O., Forrest,A.R., Gough,J., Grimmond,S.,
            Han,J.H., Hashimoto,T., Hide,W., Hofmann,O., Kamburov,A., Kaur,M.,
            Kawaji,H., Kubosaki,A., Lassmann,T., van Nimwegen,E.,
            MacPherson,C.R., Ogawa,C., Radovanovic,A., Schwartz,A.,
            Teasdale,R.D., Tegner,J., Lenhard,B., Teichmann,S.A., Arakawa,T.,
            Ninomiya,N., Murakami,K., Tagami,M., Fukuda,S., Imamura,K., Kai,C.,
            Ishihara,R., Kitazume,Y., Kawai,J., Hume,D.A., Ideker,T. and
            Hayashizaki,Y.
  TITLE     An atlas of combinatorial transcriptional regulation in mouse and
            man
  JOURNAL   Cell 140 (5), 744-752 (2010)
   PUBMED   20211142
  REMARK    Erratum:[Cell. 2010 Apr 16;141(2):369. Kamburov, Atanas [added];
            Kaur, Mandeep [added]; MacPherson, Cameron Ross [added];
            Radovanovic, Aleksandar [added]; Schwartz, Ariel [added]]
REFERENCE   8  (bases 1 to 2848)
  AUTHORS   Markson,G., Kiel,C., Hyde,R., Brown,S., Charalabous,P., Bremm,A.,
            Semple,J., Woodsmith,J., Duley,S., Salehi-Ashtiani,K., Vidal,M.,
            Komander,D., Serrano,L., Lehner,P. and Sanderson,C.M.
  TITLE     Analysis of the human E2 ubiquitin conjugating enzyme protein
            interaction network
  JOURNAL   Genome Res 19 (10), 1905-1911 (2009)
   PUBMED   19549727
REFERENCE   9  (bases 1 to 2848)
  AUTHORS   Vaquerizas,J.M., Kummerfeld,S.K., Teichmann,S.A. and Luscombe,N.M.
  TITLE     A census of human transcription factors: function, expression and
            evolution
  JOURNAL   Nat Rev Genet 10 (4), 252-263 (2009)
   PUBMED   19274049
  REMARK    Review article
REFERENCE   10 (bases 1 to 2848)
  AUTHORS   Wayne,C.M., MacLean,J.A., Cornwall,G. and Wilkinson,M.F.
  TITLE     Two novel human X-linked homeobox genes, hPEPP1 and hPEPP2,
            selectively expressed in the testis
  JOURNAL   Gene 301 (1-2), 1-11 (2002)
   PUBMED   12490318
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL590526.1, BC021719.2 and
            AC240549.4.
            
            On May 17, 2019 this sequence version replaced NM_032498.2.
            
            Summary: This gene, which encodes a transcriptional repressor, is
            one of two paralogous X-linked homeobox-containing genes and is
            highly expressed in a variety of cancers. In addition, the encoded
            protein associates with the cell membrane and with microtubules,
            and is concentrated at the leading edge of migratory cells.
            [provided by RefSeq, Dec 2015].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189661.164351.1,
                                           SRR5189661.152102.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2148093
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000371388.5/ ENSP00000360441.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1245              AL590526.1         53-1297
            1246-1257           BC021719.2         1193-1204
            1258-2848           AC240549.4         75838-77428         c
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq24"
     gene            1..2848
                     /gene="RHOXF2"
                     /gene_synonym="CT107; PEPP-2; PEPP2; THG1"
                     /note="Rhox homeobox family member 2"
                     /db_xref="GeneID:84528"
                     /db_xref="HGNC:HGNC:30011"
                     /db_xref="MIM:300447"
     exon            1..217
                     /gene="RHOXF2"
                     /gene_synonym="CT107; PEPP-2; PEPP2; THG1"
                     /inference="alignment:Splign:2.1.0"
     CDS             139..1005
                     /gene="RHOXF2"
                     /gene_synonym="CT107; PEPP-2; PEPP2; THG1"
                     /note="homeobox protein from AL590526; PEPP subfamily gene
                     2; cancer/testis antigen 107; testis homeobox gene 1;
                     paired-like homeobox protein PEPP-2"
                     /codon_start=1
                     /product="rhox homeobox family member 2"
                     /protein_id="NP_115887.1"
                     /db_xref="CCDS:CCDS14594.1"
                     /db_xref="GeneID:84528"
                     /db_xref="HGNC:HGNC:30011"
                     /db_xref="MIM:300447"
                     /translation="MEPPDQCSQYMTSLLSPAVDDEKELQDMNAMVLSLTEEVKEEEE
                     DAQPEPEQGTAAGEKLKSAGAQGGEEKDGGGEEKDGGGAGVPGHLWEGDLEGTSGSDG
                     NVEDSDQSEKEPGQQYSRPQGAVGGLEPGNAQQPNVHAFTPLQLQELERIFQREQFPS
                     EFLRRRLARSMNVTELAVQIWFENRRAKWRRHQRALMARNMLPFMAVGQPVMVTAAEA
                     ITAPLFISGMRDDYFWDHSHSSSLCFPMPPFPPPSLPLPLMLLPPMPPAGQAEFGPFP
                     FVIVPSFTFPNV"
     exon            218..629
                     /gene="RHOXF2"
                     /gene_synonym="CT107; PEPP-2; PEPP2; THG1"
                     /inference="alignment:Splign:2.1.0"
     exon            630..675
                     /gene="RHOXF2"
                     /gene_synonym="CT107; PEPP-2; PEPP2; THG1"
                     /inference="alignment:Splign:2.1.0"
     exon            676..2848
                     /gene="RHOXF2"
                     /gene_synonym="CT107; PEPP-2; PEPP2; THG1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1223..1228
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RHOXF2"
                     /gene_synonym="CT107; PEPP-2; PEPP2; THG1"
                     /note="hexamer: AATAAA"
     regulatory      1236..1241
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RHOXF2"
                     /gene_synonym="CT107; PEPP-2; PEPP2; THG1"
                     /note="hexamer: AAGAAA"
     polyA_site      1246
                     /gene="RHOXF2"
                     /gene_synonym="CT107; PEPP-2; PEPP2; THG1"
                     /note="major polyA site"
     polyA_site      1257
                     /gene="RHOXF2"
                     /gene_synonym="CT107; PEPP-2; PEPP2; THG1"
                     /note="major polyA site"
ORIGIN      
        1 ggatacactg ttgctgagtc tagacaccag aagaacgttg caggcggcga ctcacagttc
       61 tagcactgcc taggagagcg tggtggcccc agctcagaat ctgcagaagt gcacagctcc
      121 atccacacca ctcagggtat ggagcctccg gaccagtgta gccagtatat gaccagcttg
      181 ctcagccctg cagtcgacga cgagaaagaa ctacaggata tgaatgctat ggtgctgtcg
      241 cttactgaag aggtcaaaga ggaggaagag gatgcacagc ctgagcctga gcaaggcaca
      301 gcagcaggag aaaagttaaa gtcggcagga gcccaaggcg gagaagaaaa agatggcggc
      361 ggagaagaaa aagatggcgg cggcgccgga gttcctggcc acctatggga aggagacctc
      421 gagggcacca gcggcagcga tggcaacgtt gaggacagcg accagagcga gaaggaacct
      481 gggcagcagt attcgcgccc acagggcgcc gtcggggggc tggagcctgg caacgcgcag
      541 cagcccaacg tccacgcctt caccccattg cagctgcagg agctggagcg cattttccaa
      601 cgcgagcagt tccccagtga gttcctgcga aggaggctgg caagaagcat gaatgtgact
      661 gaactcgcag tgcagatttg gtttgagaat agaagagcca aatggaggag acatcagagg
      721 gcattaatgg caagaaacat gctgcccttc atggcagtgg gccagcctgt catggtaacc
      781 gcagctgagg ccataacggc acccttgttc atcagcggga tgagagatga ttacttctgg
      841 gaccacagcc attccagcag cctgtgtttc cccatgccac cctttcctcc tccgtccttg
      901 ccccttccac tcatgcttct tccacctatg ccacccgctg gccaggctga atttggccca
      961 ttcccttttg ttatcgtgcc ttctttcaca ttccccaatg tctaagggat agcctctgtg
     1021 ccactttttg ccagagtgtc tttgagccag attcatattt tgcatagcac cccatcaaaa
     1081 gtagttcatc aaatgtctat taaacgtttt aaagaaaagt acatcattga cccattttta
     1141 gggcacttgt aaaaatgttt ctataaatat gtgaagggta tgtacatttg ttttgtgtgt
     1201 cacatggggt cagtaagttc tcaataaaaa ttgttaagaa atgccattca aaccgaatgt
     1261 cacggactct cgtctcatgc agtaatcttg agtcaccatc tgggggctgt gcatgtcaca
     1321 gaattttccc atgtgcccat ggcaggctta caccgaccca gacattctct tccaccccca
     1381 cccccaacac cccatactca tctcctctct gcccccattg ataccaggta catgtgcagg
     1441 atggggtggt gtgagtccct aatgagagaa tagtgtgttt caagttactg ccagggtgga
     1501 aaacagcaaa accaaaatgg tatcgggaag gaagccatgc ccactcttaa aaattcatta
     1561 agttttttct tgctatggaa gacttcttta aagtaatttt cttgtaaaag tgtaagtgta
     1621 aataatgcca tgaaattata cacttattta atggctaaaa tggcaaattt ttatatggtt
     1681 taccacaaca aaagaaaaga aaaaatatac caaaaagttt ttaaaaagtg atcatgaaca
     1741 ttgtggtcat cctgtgaagt gattgcaatg cctgcagata aggagtgatg gttacaacag
     1801 tattttctct gaaaattatt tgatggccag tttcataatg attatgtttt cagcctgaag
     1861 gaaaattcca ttttcttggg tgagcatgaa ctttctgtca ggctgtctgc tgcttttcat
     1921 tccccacttc tctcttcaca attgtgggtg tcaagcttca gccacgcaat tgcatttggt
     1981 gtgagggttt tgcaaggaga aggaggttta ttcatacccc tgaagccaca agccttggtg
     2041 ggaataagga aagtccatga attcactatg cattaatgca tgcctcttgg ccaagtggat
     2101 tcttttttct tctccgattg agattttcct tttttttttt tttttttttt tttgctcttg
     2161 ttctcattgt attgtgcttt gtataattat ttacagtaag tcgcgcatgt cagtgtacat
     2221 tccgtctgga aattgtttcc atttggtaca ttttgtgcca gtcggtctat tcctgctcat
     2281 tattttgttt tttctacatt cagactgaaa catttggtag cctagagata ctcagaaata
     2341 ggcaaagaaa ggtaaaaggg gaggagggga gattgaaatc atacttccat tatccctccc
     2401 cgtggttatc agattcataa aatttttaca ccatcaaaag acatttttag ccatatatgt
     2461 ttcctttatg tagaaaatga gatctcctat actcagttgg catttgtttt catgtatctg
     2521 ataagtacct gttagaatta taggtcaaga tgttgtctag ctctggtaac ctagatcctg
     2581 aacttcaaag cagagttctt ggaccttaag aaattcaaat tcgtagaatt atagttagga
     2641 tgtcatttac tgaccttgaa caacacattg tttaggtagc atacaatggg actagttcaa
     2701 ggggctgggt ggagaacaag ttacaatttt ttctgatgga gaacattact ggagacagta
     2761 ccctttaaaa ctttcattcc ctttttatta gcagctaacg tcttgtatgc ctttactatg
     2821 tgacaagcaa taaaataatg tccttgga
//
