LOCUS       NM_138769               2490 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens ras homolog family member T2 (RHOT2), transcript
            variant 2, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_138769
VERSION     NM_138769.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Furnish,M., Boulton,D.P., Genther,V., Grofova,D., Ellinwood,M.L.,
            Romero,L., Lucia,M.S., Cramer,S.D. and Caino,M.C.
  TITLE     MIRO2 Regulates Prostate Cancer Cell Growth via GCN1-Dependent
            Stress Signaling
  JOURNAL   Mol Cancer Res 20 (4), 607-621 (2022)
   PUBMED   34992146
  REMARK    GeneRIF: MIRO2 Regulates Prostate Cancer Cell Growth via
            GCN1-Dependent Stress Signaling.
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   3  (bases 1 to 2490)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 2490)
  AUTHORS   Perinan,M.T., Gomez-Garre,P., Blauwendraat,C., Mir,P. and
            Bandres-Ciga,S.
  CONSRTM   International Parkinson's Disease Genomics Consortium (IPDGC)
  TITLE     The role of RHOT1 and RHOT2 genetic variation on Parkinson disease
            risk and onset
  JOURNAL   Neurobiol Aging 97, 144 (2021)
   PUBMED   32948353
  REMARK    GeneRIF: The role of RHOT1 and RHOT2 genetic variation on Parkinson
            disease risk and onset.
REFERENCE   5  (bases 1 to 2490)
  AUTHORS   Smith,K.P., Focia,P.J., Chakravarthy,S., Landahl,E.C.,
            Klosowiak,J.L., Rice,S.E. and Freymann,D.M.
  TITLE     Insight into human Miro1/2 domain organization based on the
            structure of its N-terminal GTPase
  JOURNAL   J Struct Biol 212 (3), 107656 (2020)
   PUBMED   33132189
  REMARK    GeneRIF: Insight into human Miro1/2 domain organization based on
            the structure of its N-terminal GTPase.
REFERENCE   6  (bases 1 to 2490)
  AUTHORS   Fransson,S., Ruusala,A. and Aspenstrom,P.
  TITLE     The atypical Rho GTPases Miro-1 and Miro-2 have essential roles in
            mitochondrial trafficking
  JOURNAL   Biochem Biophys Res Commun 344 (2), 500-510 (2006)
   PUBMED   16630562
  REMARK    GeneRIF: Moreover, we show that Miro interacts with the
            Kinesin-binding proteins, GRIF-1 and OIP106, suggesting that the
            Miro GTPases form a link between the mitochondria and the
            trafficking apparatus of the microtubules.
REFERENCE   7  (bases 1 to 2490)
  AUTHORS   Aspenstrom,P., Fransson,A. and Saras,J.
  TITLE     Rho GTPases have diverse effects on the organization of the actin
            filament system
  JOURNAL   Biochem J 377 (Pt 2), 327-337 (2004)
   PUBMED   14521508
REFERENCE   8  (bases 1 to 2490)
  AUTHORS   Shan,Y., Hexige,S., Guo,Z., Wan,B., Chen,K., Chen,X., Ma,L.,
            Huang,C., Zhao,S. and Yu,L.
  TITLE     Cloning and characterization of the mouse Arht2 gene which encodes
            a putative atypical GTPase
  JOURNAL   Cytogenet Genome Res 106 (1), 91-97 (2004)
   PUBMED   15218247
REFERENCE   9  (bases 1 to 2490)
  AUTHORS   Fransson,A., Ruusala,A. and Aspenstrom,P.
  TITLE     Atypical Rho GTPases have roles in mitochondrial homeostasis and
            apoptosis
  JOURNAL   J Biol Chem 278 (8), 6495-6502 (2003)
   PUBMED   12482879
REFERENCE   10 (bases 1 to 2490)
  AUTHORS   Daniels,R.J., Peden,J.F., Lloyd,C., Horsley,S.W., Clark,K.,
            Tufarelli,C., Kearney,L., Buckle,V.J., Doggett,N.A., Flint,J. and
            Higgs,D.R.
  TITLE     Sequence, structure and pathology of the fully annotated terminal 2
            Mb of the short arm of human chromosome 16
  JOURNAL   Hum Mol Genet 10 (4), 339-352 (2001)
   PUBMED   11157797
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB465261.1, AJ517413.1,
            AY207375.1 and AL834163.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_138769.2.
            
            Summary: This gene encodes a member of the Rho family of GTPases.
            The encoded protein is localized to the outer mitochondrial
            membrane and plays a role in mitochondrial trafficking and
            fusion-fission dynamics. [provided by RefSeq, Nov 2011].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC014942.1, SRR3476690.82902.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000315082.9/
                                                  ENSP00000321971.4
            RefSeq Select criteria             :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-68                DB465261.1         51-118
            69-482              AJ517413.1         1-414
            483-1372            AY207375.1         529-1418
            1373-2490           AL834163.1         1440-2557
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p13.3"
     gene            1..2490
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /note="ras homolog family member T2"
                     /db_xref="GeneID:89941"
                     /db_xref="HGNC:HGNC:21169"
                     /db_xref="MIM:613889"
     exon            1..105
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     CDS             69..1925
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /EC_number="3.6.5.-"
                     /note="isoform 2 is encoded by transcript variant 2;
                     mitochondrial Rho GTPase 2; ras homolog gene family,
                     member T2; mitochondrial Rho (MIRO) GTPase 2"
                     /codon_start=1
                     /product="mitochondrial Rho GTPase 2 isoform 2"
                     /protein_id="NP_620124.1"
                     /db_xref="CCDS:CCDS10417.1"
                     /db_xref="GeneID:89941"
                     /db_xref="HGNC:HGNC:21169"
                     /db_xref="MIM:613889"
                     /translation="MRRDVRILLLGEAQVGKTSLILSLVGEEFPEEVPPRAEEITIPA
                     DVTPEKVPTHIVDYSEAEQTDEELREEIHKANVVCVVYDVSEEATIEKIRTKWIPLVN
                     GGTTQGPRVPIILVGNKSDLRSGSSMEAVLPIMSQFPEIETCVECSAKNLRNISELFY
                     YAQKAVLHPTAPLYDPEAKQLRPACAQALTRIFRLSDQDLDQALSDEELNAFQKSCFG
                     HPLAPQALEDVKTVVCRNVAGGVREDRLTLDGFLFLNTLFIQRGRHETTWTILRRFGY
                     SDALELTADYLSPLIHVPPGCSTELNHLGYQFVQRVFEKHDQDRDGALSPVELQSLFS
                     VFPAAPWGPELPRTVRTEAGRLPLHGYLCQWTLVTYLDVRSCLGHLGYLGYPTLCEQD
                     QAHAITVTREKRLDQEKGQTQRSVLLCKVVGARGVGKSAFLQAFLGRGLGHQDTREQP
                     PGYAIDTVQVNGQEKYLILCEVGTDGLLATSLDATCDVACLMFDGSDPKSFAHCASVY
                     KHHYMDGQTPCLFVSSKADLPEGVAVSGPSPAEFCRKHRLPAPVPFSCAGPAEPSTTI
                     FTQLATMAAFPHLVHAELHPSSFWLRGLLGVVGAAVAAVLSFSLYRVLVKSQ"
     exon            106..164
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            165..246
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            247..290
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            291..344
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            345..397
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            398..506
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            507..608
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            609..707
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            708..816
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            817..937
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            938..1022
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            1023..1165
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            1166..1263
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            1264..1394
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            1395..1472
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            1473..1595
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            1596..1798
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            1799..2490
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2421..2426
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /note="hexamer: ATTAAA"
     polyA_site      2452
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /note="major polyA site"
     polyA_site      2490
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
ORIGIN      
        1 agagcgaaag gcttgaggac caggtcgggg ccgggttccg ggtcggggag cggctccggg
       61 cggcagctat gaggcgggac gtgcgcatcc tgttactggg cgaggcccag gtggggaaga
      121 cgtcgctgat cctgtccctg gtgggcgagg agttccccga ggaggtccct ccccgcgcgg
      181 aggagatcac catccccgcg gacgtcaccc cggagaaggt gcccacccac atcgtggact
      241 actcagaagc cgagcagacg gacgaggagc tgcgggagga gatccacaag gcaaacgtgg
      301 tgtgtgtggt gtatgacgtc tctgaggagg ccaccattga gaagattcga actaagtgga
      361 tcccactggt gaatgggggg accacgcagg ggcccagggt gcccatcatc ctagtgggca
      421 acaagtcaga cctgcggtcg gggagctcca tggaggccgt gctccccatc atgagccagt
      481 ttcccgagat tgagacctgc gtggagtgtt cggccaagaa cctgaggaac atctcagagc
      541 tgttctacta cgcccagaag gccgtcctgc atcccacagc ccccctctat gaccctgagg
      601 ccaagcagtt gaggcccgcg tgcgcccagg cgctgacgcg catcttcagg ctctcagatc
      661 aggacctgga ccaggcgctc agtgacgaag agctcaacgc tttccagaaa tcctgctttg
      721 ggcaccccct ggccccgcag gccctggagg acgtgaagac ggtggtgtgc aggaacgtgg
      781 cgggcggcgt gcgggaggac cggctgaccc tggatggttt cctcttcctg aacacgctct
      841 tcatccagcg cggccggcac gagaccacct ggaccatcct gcggcgcttc ggctacagcg
      901 atgccctgga gctgactgcg gactatctct cccctctgat ccacgtgccc cccggctgca
      961 gcacggagct caaccacctt ggctaccagt ttgtgcagag agtgtttgag aagcacgacc
     1021 aggaccgcga cggcgccctc tcgcccgtgg agctgcaaag ccttttcagt gtgttcccag
     1081 cagcgccctg gggccccgag ctcccacgca cagtccgcac agaggccggc cggttgcccc
     1141 tgcacggata cctctgccag tggaccctgg tgacctacct ggacgtccgg agctgccttg
     1201 gacacctagg ctacctgggc taccccaccc tctgtgagca ggaccaggcc catgccatca
     1261 cagtcactcg tgagaagagg ctggaccagg agaagggaca gacgcagcgg agcgtcctcc
     1321 tgtgcaaggt ggtaggggcc cgtggagtgg gcaagtctgc cttcctgcag gcctttctcg
     1381 gccgcggcct ggggcaccag gacacgaggg agcagcctcc cggctacgcc atcgacacgg
     1441 tgcaggtcaa tggacaggag aagtacttga tcctctgtga ggtgggcaca gatggtctgc
     1501 tggccacatc gctggacgcc acctgtgacg ttgcctgctt gatgtttgat ggcagtgacc
     1561 caaagtcctt tgcacattgt gccagcgtct acaagcacca ttacatggac gggcagaccc
     1621 cctgcctctt tgtctcctcc aaggccgacc tgcccgaagg tgtcgcggtg tctggcccat
     1681 caccggccga gttttgccgc aagcaccggc tacccgctcc cgtgccgttc tcctgtgctg
     1741 gcccagccga gcccagcacc accatcttca cccagctcgc caccatggcc gccttcccac
     1801 atttggtcca cgcagagctg catccctctt ccttctggct ccgggggctg ctgggggttg
     1861 tcggggccgc cgtggccgca gtcctcagct tctcactcta cagggtcctg gtgaagagcc
     1921 agtgaggccc ctggtaccca agccccctcc cctgacctgg gtgtgcctcg ctgctggggc
     1981 tctgcagggg cagcacagct ggggtgcagg ccaggctgcc actccgggaa cgcctttgcg
     2041 ccgggacttt ttgtttctga aggcagtcga tctgcagcgg ggccttatgc tgccatgcac
     2101 tgccctggct cctgccggac ccccagggtg ggccgtggca ggtggctgag caggagctcc
     2161 caagtgccgg ccaccgctgt cagggattgc ccacccctgg gcatcatgtg tgtggggccg
     2221 gggagcacag gtgtgggagc tggtgacccc agacccagaa ttctcagggc tctacccccc
     2281 tttcctggtc ctaggtggcc agtgggtatg aggagggctg gaaggcagag ctttgggcca
     2341 aaagcaggcg ttggggggtc ccccctcaag tttggagccg tttccgtggt tgtagcagag
     2401 gaccggaggt tgggttcctg attaaacttc actgtgtgtt ttctatctcg gatcccagtc
     2461 tctgaagaca acttgctttg attcaaccta
//
