LOCUS       NM_001352284            2377 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens ras homolog family member T2 (RHOT2), transcript
            variant 11, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001352284
VERSION     NM_001352284.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Furnish,M., Boulton,D.P., Genther,V., Grofova,D., Ellinwood,M.L.,
            Romero,L., Lucia,M.S., Cramer,S.D. and Caino,M.C.
  TITLE     MIRO2 Regulates Prostate Cancer Cell Growth via GCN1-Dependent
            Stress Signaling
  JOURNAL   Mol Cancer Res 20 (4), 607-621 (2022)
   PUBMED   34992146
  REMARK    GeneRIF: MIRO2 Regulates Prostate Cancer Cell Growth via
            GCN1-Dependent Stress Signaling.
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   3  (bases 1 to 2377)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 2377)
  AUTHORS   Perinan,M.T., Gomez-Garre,P., Blauwendraat,C., Mir,P. and
            Bandres-Ciga,S.
  CONSRTM   International Parkinson's Disease Genomics Consortium (IPDGC)
  TITLE     The role of RHOT1 and RHOT2 genetic variation on Parkinson disease
            risk and onset
  JOURNAL   Neurobiol Aging 97, 144 (2021)
   PUBMED   32948353
  REMARK    GeneRIF: The role of RHOT1 and RHOT2 genetic variation on Parkinson
            disease risk and onset.
REFERENCE   5  (bases 1 to 2377)
  AUTHORS   Smith,K.P., Focia,P.J., Chakravarthy,S., Landahl,E.C.,
            Klosowiak,J.L., Rice,S.E. and Freymann,D.M.
  TITLE     Insight into human Miro1/2 domain organization based on the
            structure of its N-terminal GTPase
  JOURNAL   J Struct Biol 212 (3), 107656 (2020)
   PUBMED   33132189
  REMARK    GeneRIF: Insight into human Miro1/2 domain organization based on
            the structure of its N-terminal GTPase.
REFERENCE   6  (bases 1 to 2377)
  AUTHORS   Fransson,S., Ruusala,A. and Aspenstrom,P.
  TITLE     The atypical Rho GTPases Miro-1 and Miro-2 have essential roles in
            mitochondrial trafficking
  JOURNAL   Biochem Biophys Res Commun 344 (2), 500-510 (2006)
   PUBMED   16630562
  REMARK    GeneRIF: Moreover, we show that Miro interacts with the
            Kinesin-binding proteins, GRIF-1 and OIP106, suggesting that the
            Miro GTPases form a link between the mitochondria and the
            trafficking apparatus of the microtubules.
REFERENCE   7  (bases 1 to 2377)
  AUTHORS   Aspenstrom,P., Fransson,A. and Saras,J.
  TITLE     Rho GTPases have diverse effects on the organization of the actin
            filament system
  JOURNAL   Biochem J 377 (Pt 2), 327-337 (2004)
   PUBMED   14521508
REFERENCE   8  (bases 1 to 2377)
  AUTHORS   Shan,Y., Hexige,S., Guo,Z., Wan,B., Chen,K., Chen,X., Ma,L.,
            Huang,C., Zhao,S. and Yu,L.
  TITLE     Cloning and characterization of the mouse Arht2 gene which encodes
            a putative atypical GTPase
  JOURNAL   Cytogenet Genome Res 106 (1), 91-97 (2004)
   PUBMED   15218247
REFERENCE   9  (bases 1 to 2377)
  AUTHORS   Fransson,A., Ruusala,A. and Aspenstrom,P.
  TITLE     Atypical Rho GTPases have roles in mitochondrial homeostasis and
            apoptosis
  JOURNAL   J Biol Chem 278 (8), 6495-6502 (2003)
   PUBMED   12482879
REFERENCE   10 (bases 1 to 2377)
  AUTHORS   Daniels,R.J., Peden,J.F., Lloyd,C., Horsley,S.W., Clark,K.,
            Tufarelli,C., Kearney,L., Buckle,V.J., Doggett,N.A., Flint,J. and
            Higgs,D.R.
  TITLE     Sequence, structure and pathology of the fully annotated terminal 2
            Mb of the short arm of human chromosome 16
  JOURNAL   Hum Mol Genet 10 (4), 339-352 (2001)
   PUBMED   11157797
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from Z92544.1.
            
            On May 31, 2019 this sequence version replaced NM_001352284.1.
            
            Summary: This gene encodes a member of the Rho family of GTPases.
            The encoded protein is localized to the outer mitochondrial
            membrane and plays a role in mitochondrial trafficking and
            fusion-fission dynamics. [provided by RefSeq, Nov 2011].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.51266.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-105               Z92544.1           25257-25361         c
            106-164             Z92544.1           25082-25140         c
            165-246             Z92544.1           24924-25005         c
            247-290             Z92544.1           24794-24837         c
            291-344             Z92544.1           23887-23940         c
            345-397             Z92544.1           23318-23370         c
            398-489             Z92544.1           23136-23227         c
            490-591             Z92544.1           22936-23037         c
            592-703             Z92544.1           22493-22604         c
            704-824             Z92544.1           22290-22410         c
            825-909             Z92544.1           21712-21796         c
            910-1052            Z92544.1           21491-21633         c
            1053-1150           Z92544.1           21312-21409         c
            1151-1281           Z92544.1           21109-21239         c
            1282-1359           Z92544.1           20927-21004         c
            1360-1482           Z92544.1           20668-20790         c
            1483-1685           Z92544.1           20363-20565         c
            1686-2377           Z92544.1           19322-20013         c
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p13.3"
     gene            1..2377
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /note="ras homolog family member T2"
                     /db_xref="GeneID:89941"
                     /db_xref="HGNC:HGNC:21169"
                     /db_xref="MIM:613889"
     exon            1..105
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            106..164
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            165..246
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            247..290
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            291..344
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            345..397
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     CDS             373..1812
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /EC_number="3.6.5.-"
                     /note="isoform 10 is encoded by transcript variant 11;
                     mitochondrial Rho GTPase 2; ras homolog gene family,
                     member T2; mitochondrial Rho (MIRO) GTPase 2"
                     /codon_start=1
                     /product="mitochondrial Rho GTPase 2 isoform 10"
                     /protein_id="NP_001339213.1"
                     /db_xref="GeneID:89941"
                     /db_xref="HGNC:HGNC:21169"
                     /db_xref="MIM:613889"
                     /translation="MGGPRRGPVGNKSDLRSGSSMEAVLPIMSQFPEIETCVECSAKN
                     LRNISELFYYAQKAVLHPTAPLYDPEAKQQKSCFGHPLAPQALEDVKTVVCRNVAGGV
                     REDRLTLDGFLFLNTLFIQRGRHETTWTILRRFGYSDALELTADYLSPLIHVPPGCST
                     ELNHLGYQFVQRVFEKHDQDRDGALSPVELQSLFSVFPAAPWGPELPRTVRTEAGRLP
                     LHGYLCQWTLVTYLDVRSCLGHLGYLGYPTLCEQDQAHAITVTREKRLDQEKGQTQRS
                     VLLCKVVGARGVGKSAFLQAFLGRGLGHQDTREQPPGYAIDTVQVNGQEKYLILCEVG
                     TDGLLATSLDATCDVACLMFDGSDPKSFAHCASVYKHHYMDGQTPCLFVSSKADLPEG
                     VAVSGPSPAEFCRKHRLPAPVPFSCAGPAEPSTTIFTQLATMAAFPHLVHAELHPSSF
                     WLRGLLGVVGAAVAAVLSFSLYRVLVKSQ"
     exon            398..489
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            490..591
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            592..703
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            704..824
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            825..909
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            910..1052
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            1053..1150
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            1151..1281
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            1282..1359
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            1360..1482
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            1483..1685
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            1686..2377
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2308..2313
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /note="hexamer: ATTAAA"
     polyA_site      2339
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /note="major polyA site"
     polyA_site      2377
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
ORIGIN      
        1 agagcgaaag gcttgaggac caggtcgggg ccgggttccg ggtcggggag cggctccggg
       61 cggcagctat gaggcgggac gtgcgcatcc tgttactggg cgaggcccag gtggggaaga
      121 cgtcgctgat cctgtccctg gtgggcgagg agttccccga ggaggtccct ccccgcgcgg
      181 aggagatcac catccccgcg gacgtcaccc cggagaaggt gcccacccac atcgtggact
      241 actcagaagc cgagcagacg gacgaggagc tgcgggagga gatccacaag gcaaacgtgg
      301 tgtgtgtggt gtatgacgtc tctgaggagg ccaccattga gaagattcga actaagtgga
      361 tcccactggt gaatgggggg accacgcagg ggcccagtgg gcaacaagtc agacctgcgg
      421 tcggggagct ccatggaggc cgtgctcccc atcatgagcc agtttcccga gattgagacc
      481 tgcgtggagt gttcggccaa gaacctgagg aacatctcag agctgttcta ctacgcccag
      541 aaggccgtcc tgcatcccac agcccccctc tatgaccctg aggccaagca gcagaaatcc
      601 tgctttgggc accccctggc cccgcaggcc ctggaggacg tgaagacggt ggtgtgcagg
      661 aacgtggcgg gcggcgtgcg ggaggaccgg ctgaccctgg atggtttcct cttcctgaac
      721 acgctcttca tccagcgcgg ccggcacgag accacctgga ccatcctgcg gcgcttcggc
      781 tacagcgatg ccctggagct gactgcggac tatctctccc ctctgatcca cgtgcccccc
      841 ggctgcagca cggagctcaa ccaccttggc taccagtttg tgcagagagt gtttgagaag
      901 cacgaccagg accgcgacgg cgccctctcg cccgtggagc tgcaaagcct tttcagtgtg
      961 ttcccagcag cgccctgggg ccccgagctc ccacgcacag tccgcacaga ggccggccgg
     1021 ttgcccctgc acggatacct ctgccagtgg accctggtga cctacctgga cgtccggagc
     1081 tgccttggac acctaggcta cctgggctac cccaccctct gtgagcagga ccaggcccat
     1141 gccatcacag tcactcgtga gaagaggctg gaccaggaga agggacagac gcagcggagc
     1201 gtcctcctgt gcaaggtggt aggggcccgt ggagtgggca agtctgcctt cctgcaggcc
     1261 tttctcggcc gcggcctggg gcaccaggac acgagggagc agcctcccgg ctacgccatc
     1321 gacacggtgc aggtcaatgg acaggagaag tacttgatcc tctgtgaggt gggcacagat
     1381 ggtctgctgg ccacatcgct ggacgccacc tgtgacgttg cctgcttgat gtttgatggc
     1441 agtgacccaa agtcctttgc acattgtgcc agcgtctaca agcaccatta catggacggg
     1501 cagaccccct gcctctttgt ctcctccaag gccgacctgc ccgaaggtgt cgcggtgtct
     1561 ggcccatcac cggccgagtt ttgccgcaag caccggctac ccgctcccgt gccgttctcc
     1621 tgtgctggcc cagccgagcc cagcaccacc atcttcaccc agctcgccac catggccgcc
     1681 ttcccacatt tggtccacgc agagctgcat ccctcttcct tctggctccg ggggctgctg
     1741 ggggttgtcg gggccgccgt ggccgcagtc ctcagcttct cactctacag ggtcctggtg
     1801 aagagccagt gaggcccctg gtacccaagc cccctcccct gacctgggtg tgcctcgctg
     1861 ctggggctct gcaggggcag cacagctggg gtgcaggcca ggctgccact ccgggaacgc
     1921 ctttgcgccg ggactttttg tttctgaagg cagtcgatct gcagcggggc cttatgctgc
     1981 catgcactgc cctggctcct gccggacccc cagggtgggc cgtggcaggt ggctgagcag
     2041 gagctcccaa gtgccggcca ccgctgtcag ggattgccca cccctgggca tcatgtgtgt
     2101 ggggccgggg agcacaggtg tgggagctgg tgaccccaga cccagaattc tcagggctct
     2161 accccccttt cctggtccta ggtggccagt gggtatgagg agggctggaa ggcagagctt
     2221 tgggccaaaa gcaggcgttg gggggtcccc cctcaagttt ggagccgttt ccgtggttgt
     2281 agcagaggac cggaggttgg gttcctgatt aaacttcact gtgtgttttc tatctcggat
     2341 cccagtctct gaagacaact tgctttgatt caaccta
//
