LOCUS       NM_001352275            2493 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens ras homolog family member T2 (RHOT2), transcript
            variant 1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001352275
VERSION     NM_001352275.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Furnish,M., Boulton,D.P., Genther,V., Grofova,D., Ellinwood,M.L.,
            Romero,L., Lucia,M.S., Cramer,S.D. and Caino,M.C.
  TITLE     MIRO2 Regulates Prostate Cancer Cell Growth via GCN1-Dependent
            Stress Signaling
  JOURNAL   Mol Cancer Res 20 (4), 607-621 (2022)
   PUBMED   34992146
  REMARK    GeneRIF: MIRO2 Regulates Prostate Cancer Cell Growth via
            GCN1-Dependent Stress Signaling.
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   3  (bases 1 to 2493)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 2493)
  AUTHORS   Perinan,M.T., Gomez-Garre,P., Blauwendraat,C., Mir,P. and
            Bandres-Ciga,S.
  CONSRTM   International Parkinson's Disease Genomics Consortium (IPDGC)
  TITLE     The role of RHOT1 and RHOT2 genetic variation on Parkinson disease
            risk and onset
  JOURNAL   Neurobiol Aging 97, 144 (2021)
   PUBMED   32948353
  REMARK    GeneRIF: The role of RHOT1 and RHOT2 genetic variation on Parkinson
            disease risk and onset.
REFERENCE   5  (bases 1 to 2493)
  AUTHORS   Smith,K.P., Focia,P.J., Chakravarthy,S., Landahl,E.C.,
            Klosowiak,J.L., Rice,S.E. and Freymann,D.M.
  TITLE     Insight into human Miro1/2 domain organization based on the
            structure of its N-terminal GTPase
  JOURNAL   J Struct Biol 212 (3), 107656 (2020)
   PUBMED   33132189
  REMARK    GeneRIF: Insight into human Miro1/2 domain organization based on
            the structure of its N-terminal GTPase.
REFERENCE   6  (bases 1 to 2493)
  AUTHORS   Fransson,S., Ruusala,A. and Aspenstrom,P.
  TITLE     The atypical Rho GTPases Miro-1 and Miro-2 have essential roles in
            mitochondrial trafficking
  JOURNAL   Biochem Biophys Res Commun 344 (2), 500-510 (2006)
   PUBMED   16630562
  REMARK    GeneRIF: Moreover, we show that Miro interacts with the
            Kinesin-binding proteins, GRIF-1 and OIP106, suggesting that the
            Miro GTPases form a link between the mitochondria and the
            trafficking apparatus of the microtubules.
REFERENCE   7  (bases 1 to 2493)
  AUTHORS   Aspenstrom,P., Fransson,A. and Saras,J.
  TITLE     Rho GTPases have diverse effects on the organization of the actin
            filament system
  JOURNAL   Biochem J 377 (Pt 2), 327-337 (2004)
   PUBMED   14521508
REFERENCE   8  (bases 1 to 2493)
  AUTHORS   Shan,Y., Hexige,S., Guo,Z., Wan,B., Chen,K., Chen,X., Ma,L.,
            Huang,C., Zhao,S. and Yu,L.
  TITLE     Cloning and characterization of the mouse Arht2 gene which encodes
            a putative atypical GTPase
  JOURNAL   Cytogenet Genome Res 106 (1), 91-97 (2004)
   PUBMED   15218247
REFERENCE   9  (bases 1 to 2493)
  AUTHORS   Fransson,A., Ruusala,A. and Aspenstrom,P.
  TITLE     Atypical Rho GTPases have roles in mitochondrial homeostasis and
            apoptosis
  JOURNAL   J Biol Chem 278 (8), 6495-6502 (2003)
   PUBMED   12482879
REFERENCE   10 (bases 1 to 2493)
  AUTHORS   Daniels,R.J., Peden,J.F., Lloyd,C., Horsley,S.W., Clark,K.,
            Tufarelli,C., Kearney,L., Buckle,V.J., Doggett,N.A., Flint,J. and
            Higgs,D.R.
  TITLE     Sequence, structure and pathology of the fully annotated terminal 2
            Mb of the short arm of human chromosome 16
  JOURNAL   Hum Mol Genet 10 (4), 339-352 (2001)
   PUBMED   11157797
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from Z92544.1.
            
            On May 31, 2019 this sequence version replaced NM_001352275.1.
            
            Summary: This gene encodes a member of the Rho family of GTPases.
            The encoded protein is localized to the outer mitochondrial
            membrane and plays a role in mitochondrial trafficking and
            fusion-fission dynamics. [provided by RefSeq, Nov 2011].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.4701.1,
                                           SRR1803612.156165.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-105               Z92544.1           25257-25361         c
            106-164             Z92544.1           25082-25140         c
            165-246             Z92544.1           24924-25005         c
            247-290             Z92544.1           24794-24837         c
            291-344             Z92544.1           23887-23940         c
            345-397             Z92544.1           23318-23370         c
            398-506             Z92544.1           23136-23244         c
            507-608             Z92544.1           22936-23037         c
            609-707             Z92544.1           22720-22818         c
            708-819             Z92544.1           22493-22604         c
            820-940             Z92544.1           22290-22410         c
            941-1025            Z92544.1           21712-21796         c
            1026-1168           Z92544.1           21491-21633         c
            1169-1266           Z92544.1           21312-21409         c
            1267-1397           Z92544.1           21109-21239         c
            1398-1475           Z92544.1           20927-21004         c
            1476-1598           Z92544.1           20668-20790         c
            1599-1801           Z92544.1           20363-20565         c
            1802-2493           Z92544.1           19322-20013         c
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p13.3"
     gene            1..2493
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /note="ras homolog family member T2"
                     /db_xref="GeneID:89941"
                     /db_xref="HGNC:HGNC:21169"
                     /db_xref="MIM:613889"
     exon            1..105
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     CDS             69..1928
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /EC_number="3.6.5.-"
                     /note="isoform 1 is encoded by transcript variant 1;
                     mitochondrial Rho GTPase 2; ras homolog gene family,
                     member T2; mitochondrial Rho (MIRO) GTPase 2"
                     /codon_start=1
                     /product="mitochondrial Rho GTPase 2 isoform 1"
                     /protein_id="NP_001339204.1"
                     /db_xref="CCDS:CCDS92073.1"
                     /db_xref="GeneID:89941"
                     /db_xref="HGNC:HGNC:21169"
                     /db_xref="MIM:613889"
                     /translation="MRRDVRILLLGEAQVGKTSLILSLVGEEFPEEVPPRAEEITIPA
                     DVTPEKVPTHIVDYSEAEQTDEELREEIHKANVVCVVYDVSEEATIEKIRTKWIPLVN
                     GGTTQGPRVPIILVGNKSDLRSGSSMEAVLPIMSQFPEIETCVECSAKNLRNISELFY
                     YAQKAVLHPTAPLYDPEAKQLRPACAQALTRIFRLSDQDLDQALSDEELNAFQQKSCF
                     GHPLAPQALEDVKTVVCRNVAGGVREDRLTLDGFLFLNTLFIQRGRHETTWTILRRFG
                     YSDALELTADYLSPLIHVPPGCSTELNHLGYQFVQRVFEKHDQDRDGALSPVELQSLF
                     SVFPAAPWGPELPRTVRTEAGRLPLHGYLCQWTLVTYLDVRSCLGHLGYLGYPTLCEQ
                     DQAHAITVTREKRLDQEKGQTQRSVLLCKVVGARGVGKSAFLQAFLGRGLGHQDTREQ
                     PPGYAIDTVQVNGQEKYLILCEVGTDGLLATSLDATCDVACLMFDGSDPKSFAHCASV
                     YKHHYMDGQTPCLFVSSKADLPEGVAVSGPSPAEFCRKHRLPAPVPFSCAGPAEPSTT
                     IFTQLATMAAFPHLVHAELHPSSFWLRGLLGVVGAAVAAVLSFSLYRVLVKSQ"
     exon            106..164
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            165..246
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            247..290
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            291..344
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            345..397
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            398..506
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            507..608
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            609..707
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            708..819
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            820..940
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            941..1025
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            1026..1168
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            1169..1266
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            1267..1397
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            1398..1475
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            1476..1598
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            1599..1801
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     exon            1802..2493
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2424..2429
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /note="hexamer: ATTAAA"
     polyA_site      2455
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
                     /note="major polyA site"
     polyA_site      2493
                     /gene="RHOT2"
                     /gene_synonym="ARHT2; C16orf39; MIRO-2; MIRO2; RASL"
ORIGIN      
        1 agagcgaaag gcttgaggac caggtcgggg ccgggttccg ggtcggggag cggctccggg
       61 cggcagctat gaggcgggac gtgcgcatcc tgttactggg cgaggcccag gtggggaaga
      121 cgtcgctgat cctgtccctg gtgggcgagg agttccccga ggaggtccct ccccgcgcgg
      181 aggagatcac catccccgcg gacgtcaccc cggagaaggt gcccacccac atcgtggact
      241 actcagaagc cgagcagacg gacgaggagc tgcgggagga gatccacaag gcaaacgtgg
      301 tgtgtgtggt gtatgacgtc tctgaggagg ccaccattga gaagattcga actaagtgga
      361 tcccactggt gaatgggggg accacgcagg ggcccagggt gcccatcatc ctagtgggca
      421 acaagtcaga cctgcggtcg gggagctcca tggaggccgt gctccccatc atgagccagt
      481 ttcccgagat tgagacctgc gtggagtgtt cggccaagaa cctgaggaac atctcagagc
      541 tgttctacta cgcccagaag gccgtcctgc atcccacagc ccccctctat gaccctgagg
      601 ccaagcagtt gaggcccgcg tgcgcccagg cgctgacgcg catcttcagg ctctcagatc
      661 aggacctgga ccaggcgctc agtgacgaag agctcaacgc tttccagcag aaatcctgct
      721 ttgggcaccc cctggccccg caggccctgg aggacgtgaa gacggtggtg tgcaggaacg
      781 tggcgggcgg cgtgcgggag gaccggctga ccctggatgg tttcctcttc ctgaacacgc
      841 tcttcatcca gcgcggccgg cacgagacca cctggaccat cctgcggcgc ttcggctaca
      901 gcgatgccct ggagctgact gcggactatc tctcccctct gatccacgtg ccccccggct
      961 gcagcacgga gctcaaccac cttggctacc agtttgtgca gagagtgttt gagaagcacg
     1021 accaggaccg cgacggcgcc ctctcgcccg tggagctgca aagccttttc agtgtgttcc
     1081 cagcagcgcc ctggggcccc gagctcccac gcacagtccg cacagaggcc ggccggttgc
     1141 ccctgcacgg atacctctgc cagtggaccc tggtgaccta cctggacgtc cggagctgcc
     1201 ttggacacct aggctacctg ggctacccca ccctctgtga gcaggaccag gcccatgcca
     1261 tcacagtcac tcgtgagaag aggctggacc aggagaaggg acagacgcag cggagcgtcc
     1321 tcctgtgcaa ggtggtaggg gcccgtggag tgggcaagtc tgccttcctg caggcctttc
     1381 tcggccgcgg cctggggcac caggacacga gggagcagcc tcccggctac gccatcgaca
     1441 cggtgcaggt caatggacag gagaagtact tgatcctctg tgaggtgggc acagatggtc
     1501 tgctggccac atcgctggac gccacctgtg acgttgcctg cttgatgttt gatggcagtg
     1561 acccaaagtc ctttgcacat tgtgccagcg tctacaagca ccattacatg gacgggcaga
     1621 ccccctgcct ctttgtctcc tccaaggccg acctgcccga aggtgtcgcg gtgtctggcc
     1681 catcaccggc cgagttttgc cgcaagcacc ggctacccgc tcccgtgccg ttctcctgtg
     1741 ctggcccagc cgagcccagc accaccatct tcacccagct cgccaccatg gccgccttcc
     1801 cacatttggt ccacgcagag ctgcatccct cttccttctg gctccggggg ctgctggggg
     1861 ttgtcggggc cgccgtggcc gcagtcctca gcttctcact ctacagggtc ctggtgaaga
     1921 gccagtgagg cccctggtac ccaagccccc tcccctgacc tgggtgtgcc tcgctgctgg
     1981 ggctctgcag gggcagcaca gctggggtgc aggccaggct gccactccgg gaacgccttt
     2041 gcgccgggac tttttgtttc tgaaggcagt cgatctgcag cggggcctta tgctgccatg
     2101 cactgccctg gctcctgccg gacccccagg gtgggccgtg gcaggtggct gagcaggagc
     2161 tcccaagtgc cggccaccgc tgtcagggat tgcccacccc tgggcatcat gtgtgtgggg
     2221 ccggggagca caggtgtggg agctggtgac cccagaccca gaattctcag ggctctaccc
     2281 ccctttcctg gtcctaggtg gccagtgggt atgaggaggg ctggaaggca gagctttggg
     2341 ccaaaagcag gcgttggggg gtcccccctc aagtttggag ccgtttccgt ggttgtagca
     2401 gaggaccgga ggttgggttc ctgattaaac ttcactgtgt gttttctatc tcggatccca
     2461 gtctctgaag acaacttgct ttgattcaac cta
//
