LOCUS       NM_001033566            3165 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens ras homolog family member T1 (RHOT1), transcript
            variant 2, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001033566
VERSION     NM_001033566.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Jing,M., Xiong,X., Mao,X., Song,Q., Zhang,L., Ouyang,Y., Pang,Y.,
            Fu,Y. and Yan,W.
  TITLE     HMGB1 promotes mitochondrial transfer between hepatocellular
            carcinoma cells through RHOT1 and RAC1 under hypoxia
  JOURNAL   Cell Death Dis 15 (2), 155 (2024)
   PUBMED   38378644
  REMARK    GeneRIF: HMGB1 promotes mitochondrial transfer between
            hepatocellular carcinoma cells through RHOT1 and RAC1 under
            hypoxia.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Baltrusaitis,E.E., Ravitch,E.E., Fenton,A.R., Perez,T.A.,
            Holzbaur,E.L.F. and Dominguez,R.
  TITLE     Interaction between the mitochondrial adaptor MIRO and the motor
            adaptor TRAK
  JOURNAL   J Biol Chem 299 (12), 105441 (2023)
   PUBMED   37949220
  REMARK    GeneRIF: Interaction between the mitochondrial adaptor MIRO and the
            motor adaptor TRAK.
REFERENCE   3  (bases 1 to 3165)
  AUTHORS   Peng,Y.Y., Sun,D. and Xin,Y.
  TITLE     Hsa_circ_0005230 is up-regulated and promotes gastric cancer cell
            invasion and migration via regulating the miR-1299/RHOT1 axis
  JOURNAL   Bioengineered 13 (3), 5046-5063 (2022)
   PUBMED   35170374
  REMARK    GeneRIF: Hsa_circ_0005230 is up-regulated and promotes gastric
            cancer cell invasion and migration via regulating the
            miR-1299/RHOT1 axis.
REFERENCE   4  (bases 1 to 3165)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   5  (bases 1 to 3165)
  AUTHORS   Fatiga,F.F., Wang,L.J., Hsu,T., Capuno,J.I. and Fu,C.Y.
  TITLE     Miro1 functions as an inhibitory regulator of MFN at elevated
            mitochondrial Ca2+ levels
  JOURNAL   J Cell Biochem 122 (12), 1848-1862 (2021)
   PUBMED   34431132
  REMARK    GeneRIF: Miro1 functions as an inhibitory regulator of MFN at
            elevated mitochondrial Ca(2+) levels.
REFERENCE   6  (bases 1 to 3165)
  AUTHORS   MacAskill,A.F., Brickley,K., Stephenson,F.A. and Kittler,J.T.
  TITLE     GTPase dependent recruitment of Grif-1 by Miro1 regulates
            mitochondrial trafficking in hippocampal neurons
  JOURNAL   Mol Cell Neurosci 40 (3), 301-312 (2009)
   PUBMED   19103291
  REMARK    GeneRIF: These data suggest that Miro1 and the kinesin adaptor
            Grif-1 play an important role in regulating mitochondrial transport
            in neurons.
REFERENCE   7  (bases 1 to 3165)
  AUTHORS   Saotome,M., Safiulina,D., Szabadkai,G., Das,S., Fransson,A.,
            Aspenstrom,P., Rizzuto,R. and Hajnoczky,G.
  TITLE     Bidirectional Ca2+-dependent control of mitochondrial dynamics by
            the Miro GTPase
  JOURNAL   Proc Natl Acad Sci U S A 105 (52), 20728-20733 (2008)
   PUBMED   19098100
  REMARK    GeneRIF: Miro1,2 proteins serve as a [Ca(2+)](c)-sensitive switch
            and bifunctional regulator for both the motility and fusion-fission
            dynamics of the mitochondria.
REFERENCE   8  (bases 1 to 3165)
  AUTHORS   Fransson,S., Ruusala,A. and Aspenstrom,P.
  TITLE     The atypical Rho GTPases Miro-1 and Miro-2 have essential roles in
            mitochondrial trafficking
  JOURNAL   Biochem Biophys Res Commun 344 (2), 500-510 (2006)
   PUBMED   16630562
  REMARK    GeneRIF: Moreover, we show that Miro interacts with the
            Kinesin-binding proteins, GRIF-1 and OIP106, suggesting that the
            Miro GTPases form a link between the mitochondria and the
            trafficking apparatus of the microtubules.
REFERENCE   9  (bases 1 to 3165)
  AUTHORS   Aspenstrom,P., Fransson,A. and Saras,J.
  TITLE     Rho GTPases have diverse effects on the organization of the actin
            filament system
  JOURNAL   Biochem J 377 (Pt 2), 327-337 (2004)
   PUBMED   14521508
REFERENCE   10 (bases 1 to 3165)
  AUTHORS   Fransson,A., Ruusala,A. and Aspenstrom,P.
  TITLE     Atypical Rho GTPases have roles in mitochondrial homeostasis and
            apoptosis
  JOURNAL   J Biol Chem 278 (8), 6495-6502 (2003)
   PUBMED   12482879
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC060781.1, BC041114.1, AJ496730.1 and AA772711.1.
            
            On Aug 13, 2020 this sequence version replaced NM_001033566.2.
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon in the 3' coding region, compared to variant 1. It encodes
            isoform 2, which is shorter than isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853561.12351.1,
                                           SRR11853559.13852.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000545287.7/ ENSP00000439737.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-18                BC060781.1         49-66
            19-1930             BC041114.1         1-1912
            1931-2053           AJ496730.1         2063-2185
            2054-2873           BC041114.1         1913-2732
            2874-3165           AA772711.1         51-342              c
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q11.2"
     gene            1..3165
                     /gene="RHOT1"
                     /gene_synonym="ARHT1; MIRO-1; MIRO1"
                     /note="ras homolog family member T1"
                     /db_xref="GeneID:55288"
                     /db_xref="HGNC:HGNC:21168"
                     /db_xref="MIM:613888"
     exon            1..228
                     /gene="RHOT1"
                     /gene_synonym="ARHT1; MIRO-1; MIRO1"
                     /inference="alignment:Splign:2.1.0"
     CDS             192..2171
                     /gene="RHOT1"
                     /gene_synonym="ARHT1; MIRO-1; MIRO1"
                     /note="isoform 2 is encoded by transcript variant 2;
                     rac-GTP binding protein-like protein; ras homolog gene
                     family, member T1; mitochondrial Rho (MIRO) GTPase 1"
                     /codon_start=1
                     /product="mitochondrial Rho GTPase 1 isoform 2"
                     /protein_id="NP_001028738.1"
                     /db_xref="CCDS:CCDS32611.1"
                     /db_xref="GeneID:55288"
                     /db_xref="HGNC:HGNC:21168"
                     /db_xref="MIM:613888"
                     /translation="MKKDVRILLVGEPRVGKTSLIMSLVSEEFPEEVPPRAEEITIPA
                     DVTPERVPTHIVDYSEAEQSDEQLHQEISQANVICIVYAVNNKHSIDKVTSRWIPLIN
                     ERTDKDSRLPLILVGNKSDLVEYSSMETILPIMNQYTEIETCVECSAKNLKNISELFY
                     YAQKAVLHPTGPLYCPEEKEMKPACIKALTRIFKISDQDNDGTLNDAELNFFQRICFN
                     TPLAPQALEDVKNVVRKHISDGVADSGLTLKGFLFLHTLFIQRGRHETTWTVLRRFGY
                     DDDLDLTPEYLFPLLKIPPDCTTELNHHAYLFLQSTFDKHDLDRDCALSPDELKDLFK
                     VFPYIPWGPDVNNTVCTNERGWITYQGFLSQWTLTTYLDVQRCLEYLGYLGYSILTEQ
                     ESQASAVTVTRDKKIDLQKKQTQRNVFRCNVIGVKNCGKSGVLQALLGRNLMRQKKIR
                     EDHKSYYAINTVYVYGQEKYLLLHDISESEFLTEAEIICDVVCLVYDVSNPKSFEYCA
                     RIFKQHFMDSRIPCLIVAAKSDLHEVKQEYSISPTDFCRKHKMPPPQAFTCNTADAPS
                     KDIFVKLTTMAMYPHARLRCMCTCNRCTFCICQNFLNSDLLQSVKNKIFTAVLNRHVT
                     QADLKSSTFWLRASFGATVFAVLGFAMYKALLKQR"
     exon            229..287
                     /gene="RHOT1"
                     /gene_synonym="ARHT1; MIRO-1; MIRO1"
                     /inference="alignment:Splign:2.1.0"
     exon            288..369
                     /gene="RHOT1"
                     /gene_synonym="ARHT1; MIRO-1; MIRO1"
                     /inference="alignment:Splign:2.1.0"
     exon            370..413
                     /gene="RHOT1"
                     /gene_synonym="ARHT1; MIRO-1; MIRO1"
                     /inference="alignment:Splign:2.1.0"
     exon            414..467
                     /gene="RHOT1"
                     /gene_synonym="ARHT1; MIRO-1; MIRO1"
                     /inference="alignment:Splign:2.1.0"
     exon            468..520
                     /gene="RHOT1"
                     /gene_synonym="ARHT1; MIRO-1; MIRO1"
                     /inference="alignment:Splign:2.1.0"
     exon            521..629
                     /gene="RHOT1"
                     /gene_synonym="ARHT1; MIRO-1; MIRO1"
                     /inference="alignment:Splign:2.1.0"
     exon            630..731
                     /gene="RHOT1"
                     /gene_synonym="ARHT1; MIRO-1; MIRO1"
                     /inference="alignment:Splign:2.1.0"
     exon            732..830
                     /gene="RHOT1"
                     /gene_synonym="ARHT1; MIRO-1; MIRO1"
                     /inference="alignment:Splign:2.1.0"
     exon            831..939
                     /gene="RHOT1"
                     /gene_synonym="ARHT1; MIRO-1; MIRO1"
                     /inference="alignment:Splign:2.1.0"
     exon            940..1060
                     /gene="RHOT1"
                     /gene_synonym="ARHT1; MIRO-1; MIRO1"
                     /inference="alignment:Splign:2.1.0"
     exon            1061..1145
                     /gene="RHOT1"
                     /gene_synonym="ARHT1; MIRO-1; MIRO1"
                     /inference="alignment:Splign:2.1.0"
     exon            1146..1291
                     /gene="RHOT1"
                     /gene_synonym="ARHT1; MIRO-1; MIRO1"
                     /inference="alignment:Splign:2.1.0"
     exon            1292..1392
                     /gene="RHOT1"
                     /gene_synonym="ARHT1; MIRO-1; MIRO1"
                     /inference="alignment:Splign:2.1.0"
     exon            1393..1523
                     /gene="RHOT1"
                     /gene_synonym="ARHT1; MIRO-1; MIRO1"
                     /inference="alignment:Splign:2.1.0"
     exon            1524..1607
                     /gene="RHOT1"
                     /gene_synonym="ARHT1; MIRO-1; MIRO1"
                     /inference="alignment:Splign:2.1.0"
     exon            1608..1727
                     /gene="RHOT1"
                     /gene_synonym="ARHT1; MIRO-1; MIRO1"
                     /inference="alignment:Splign:2.1.0"
     exon            1728..1930
                     /gene="RHOT1"
                     /gene_synonym="ARHT1; MIRO-1; MIRO1"
                     /inference="alignment:Splign:2.1.0"
     exon            1931..2053
                     /gene="RHOT1"
                     /gene_synonym="ARHT1; MIRO-1; MIRO1"
                     /inference="alignment:Splign:2.1.0"
     exon            2054..3165
                     /gene="RHOT1"
                     /gene_synonym="ARHT1; MIRO-1; MIRO1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3141..3146
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RHOT1"
                     /gene_synonym="ARHT1; MIRO-1; MIRO1"
                     /note="hexamer: AATAAA"
     polyA_site      3165
                     /gene="RHOT1"
                     /gene_synonym="ARHT1; MIRO-1; MIRO1"
                     /note="major polyA site"
ORIGIN      
        1 acagcccgct gggccggagg aggcggagct ggcgctgtcc cggctctctt gcggggaagc
       61 aactgagggg gcggcgcggc gggccccggc ggccgaagag gctggcaggt ggcgccgtgg
      121 ggtgggtgct cctggtgaga ggagtccact ccgtgcgtgc gggcggaggc cggcccccga
      181 gagccgccga catgaagaaa gacgtgcgga tcctgctggt gggagaacct agagttggga
      241 agacatcact gattatgtct ctggtcagtg aagaatttcc agaagaggtt cctccccggg
      301 cagaagaaat caccattcca gctgatgtca ccccagagag agttccaaca cacattgtag
      361 attactcaga agcagaacag agtgatgaac aacttcatca agaaatatct caggctaatg
      421 tcatctgtat agtgtatgcc gttaacaaca agcattctat tgataaggta acaagtcgat
      481 ggattcctct cataaatgaa agaacagaca aagacagcag gctgccttta atattggttg
      541 ggaacaaatc tgatctggtg gaatatagta gtatggagac catccttcct attatgaacc
      601 agtatacaga aatagaaacc tgtgtggagt gttcagcgaa aaacctgaag aacatatcag
      661 agctctttta ttacgcacag aaagctgttc ttcatcctac agggcccctg tactgcccag
      721 aggagaagga gatgaaacca gcttgtataa aagcccttac tcgtatattt aaaatatctg
      781 atcaagataa tgatggtact ctcaatgatg ctgaactcaa cttctttcag aggatttgtt
      841 tcaacactcc attagctcct caagctctgg aggatgtcaa gaatgtagtc agaaaacata
      901 taagtgatgg tgtggctgac agtgggttga ccctgaaagg ttttctcttt ttacacacac
      961 tttttatcca gagagggaga cacgaaacta cttggactgt gcttcgacga tttggttatg
     1021 atgatgacct ggatttgaca cctgaatatt tgttccccct gctgaaaata cctcctgatt
     1081 gcactactga attaaatcat catgcatatt tatttctcca aagcaccttt gacaagcatg
     1141 atttggatag agactgtgct ttgtcacctg atgagcttaa agatttattt aaagttttcc
     1201 cttacatacc ttgggggcca gatgtgaata acacagtttg taccaatgaa agaggctgga
     1261 taacctacca gggattcctt tcccagtgga cgctcacgac ttatttagat gtacagcggt
     1321 gcctggaata tttgggctat ctaggctatt caatattgac tgagcaagag tctcaagctt
     1381 cagctgttac agtgacaaga gataaaaaga tagacctgca gaaaaaacaa actcaaagaa
     1441 atgtgttcag atgtaatgta attggagtga aaaactgtgg gaaaagtgga gttcttcagg
     1501 ctcttcttgg aagaaactta atgaggcaga agaaaattcg tgaagatcat aaatcctact
     1561 atgcgattaa cactgtttat gtatatggac aagagaaata cttgttgttg catgatatct
     1621 cagaatcgga atttctaact gaagctgaaa tcatttgtga tgttgtatgc ctggtatatg
     1681 atgtcagcaa tcccaaatcc tttgaatact gtgccaggat ttttaagcaa cactttatgg
     1741 acagcagaat accttgctta atcgtagctg caaagtcaga cctgcatgaa gttaaacaag
     1801 aatacagtat ttcacctact gatttctgca ggaaacacaa aatgcctcca ccacaagcct
     1861 tcacttgcaa tactgctgat gcccccagta aggatatctt tgttaaattg acaacaatgg
     1921 ccatgtatcc ccatgcccgg ttacgctgta tgtgcacctg caacaggtgt acattttgca
     1981 tctgtcagaa cttcctcaac tcagacttgc tgcaatctgt aaagaacaaa atcttcactg
     2041 cagttcttaa caggcacgtg acacaagctg acctcaagag ctccacgttt tggcttcgag
     2101 caagttttgg tgctactgtt tttgcagttt tgggctttgc tatgtacaaa gcattattga
     2161 aacagcgatg atataaaaag aaatactgtc cctaccaaaa acaaatactt ttatgtacat
     2221 tctgaatgct ttaagttctg ctagaattat tgagatattt atacatgcag agttacttta
     2281 ttaatatttg taattcatgc ataagagtat tttaatgata gttataactg cagtattggc
     2341 tagcatatgg aaagaaaaca gctaacagcc aaactaaaat ggctaaattc cagaggccaa
     2401 aagggaatat tttgtaaata tatgtacata ttcaggcaag atatggtctc ccaagctgag
     2461 ttctagaaat gatgtttcta gacatttcta agtggtattg ttagtgctca cttggctcac
     2521 tcttctaggt ttaagttagc ccagagattg tatttactca tggatcactt tatttatttc
     2581 acatttactc agaatgatcc tttgggttct ataaggacat aaggtacaat ttgccattgt
     2641 ctctccattt ttaaaaacat acaagtcagt gtcagcttac caacatgaca ttttttcagt
     2701 cagttgtggt aggccagcct tgaagccatc gcacagtcta gaaacttgtg tagctgagtg
     2761 tgcagctcac ctttaagggt gaagttaggt aaaagcaatt agcagaggcg ttatctatgt
     2821 gattatgttg cttccttgtc agtatgttga attttatagc cctttcaatg aaataaaaaa
     2881 aaaatttgta tattaccaat gtttttagtt taaataaaga gtcaccctta ctactgttga
     2941 atttcatccc aagtgtaaat cattctataa tggctgtgtc tgttatagta tattacagta
     3001 actgcatgtg tcaccaagtg ttctatatca ggctaggata acctagaggc agtaattttt
     3061 taaatgataa aataaatcta atgaatataa actctcatga taaacctatt ttttccatca
     3121 tcagcctttt caagtattta aataaataac tgctgtgtac tgtga
//
