LOCUS       NM_020663               3556 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens ras homolog family member J (RHOJ), mRNA.
ACCESSION   NM_020663
VERSION     NM_020663.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Bou Malhab,L.J., Nair,V.A., Qaisar,R., Pintus,G. and
            Abdel-Rahman,W.M.
  TITLE     Towards Understanding the Development of Breast Cancer: The Role of
            RhoJ in the Obesity Microenvironment
  JOURNAL   Cells 13 (2), 174 (2024)
   PUBMED   38247865
  REMARK    GeneRIF: Towards Understanding the Development of Breast Cancer:
            The Role of RhoJ in the Obesity Microenvironment.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   Ma,Z., Sun,Q., Zhang,C., Zheng,Q., Liu,Y., Xu,H., He,Y., Yao,C.,
            Chen,J. and Xia,H.
  TITLE     RHOJ Induces Epithelial-to-Mesenchymal Transition by IL-6/STAT3 to
            Promote Invasion and Metastasis in Gastric Cancer
  JOURNAL   Int J Biol Sci 19 (14), 4411-4426 (2023)
   PUBMED   37781036
  REMARK    GeneRIF: RHOJ Induces Epithelial-to-Mesenchymal Transition by
            IL-6/STAT3 to Promote Invasion and Metastasis in Gastric Cancer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3556)
  AUTHORS   Nozaki,M. and Nishizuka,M.
  TITLE     Repression of RhoJ expression promotes TGF-beta-mediated EMT in
            human non-small-cell lung cancer A549cells
  JOURNAL   Biochem Biophys Res Commun 566, 94-100 (2021)
   PUBMED   34119829
  REMARK    GeneRIF: Repression of RhoJ expression promotes TGF-beta-mediated
            EMT in human non-small-cell lung cancer A549cells.
REFERENCE   4  (bases 1 to 3556)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 3556)
  AUTHORS   Leszczynska,K., Kaur,S., Wilson,E., Bicknell,R. and Heath,V.L.
  TITLE     The role of RhoJ in endothelial cell biology and angiogenesis
  JOURNAL   Biochem Soc Trans 39 (6), 1606-1611 (2011)
   PUBMED   22103495
  REMARK    Review article
REFERENCE   6  (bases 1 to 3556)
  AUTHORS   Yuan,L., Sacharidou,A., Stratman,A.N., Le Bras,A., Zwiers,P.J.,
            Spokes,K., Bhasin,M., Shih,S.C., Nagy,J.A., Molema,G., Aird,W.C.,
            Davis,G.E. and Oettgen,P.
  TITLE     RhoJ is an endothelial cell-restricted Rho GTPase that mediates
            vascular morphogenesis and is regulated by the transcription factor
            ERG
  JOURNAL   Blood 118 (4), 1145-1153 (2011)
   PUBMED   21628409
  REMARK    GeneRIF: Our study supports a novel role for the Rho family member
            RhoJ in ednothelial cell morphogenesis and in particular in lumen
            formation
REFERENCE   7  (bases 1 to 3556)
  AUTHORS   Kaur,S., Leszczynska,K., Abraham,S., Scarcia,M., Hiltbrunner,S.,
            Marshall,C.J., Mavria,G., Bicknell,R. and Heath,V.L.
  TITLE     RhoJ/TCL regulates endothelial motility and tube formation and
            modulates actomyosin contractility and focal adhesion numbers
  JOURNAL   Arterioscler Thromb Vasc Biol 31 (3), 657-664 (2011)
   PUBMED   21148427
  REMARK    GeneRIF: RhoJ is endothelial-expressed in vivo, activated by
            vascular endothelial growth factor, localizes to focal adhesions,
            regulates endothelial cell migration and tube formation, and
            modulates actomyosin contractility and focal adhesion numbers.
REFERENCE   8  (bases 1 to 3556)
  AUTHORS   Barrios-Rodiles,M., Brown,K.R., Ozdamar,B., Bose,R., Liu,Z.,
            Donovan,R.S., Shinjo,F., Liu,Y., Dembowy,J., Taylor,I.W., Luga,V.,
            Przulj,N., Robinson,M., Suzuki,H., Hayashizaki,Y., Jurisica,I. and
            Wrana,J.L.
  TITLE     High-throughput mapping of a dynamic signaling network in mammalian
            cells
  JOURNAL   Science 307 (5715), 1621-1625 (2005)
   PUBMED   15761153
REFERENCE   9  (bases 1 to 3556)
  AUTHORS   de Toledo,M., Senic-Matuglia,F., Salamero,J., Uze,G., Comunale,F.,
            Fort,P. and Blangy,A.
  TITLE     The GTP/GDP cycling of rho GTPase TCL is an essential regulator of
            the early endocytic pathway
  JOURNAL   Mol Biol Cell 14 (12), 4846-4856 (2003)
   PUBMED   12960428
  REMARK    GeneRIF: TCL is essential for clathrin-dependent endocytosed
            receptors to enter the early/sorting endosomes
            Erratum:[Mol Biol Cell. 2004 Apr;15(4):preceding table of contents]
REFERENCE   10 (bases 1 to 3556)
  AUTHORS   Vignal,E., De Toledo,M., Comunale,F., Ladopoulou,A.,
            Gauthier-Rouviere,C., Blangy,A. and Fort,P.
  TITLE     Characterization of TCL, a new GTPase of the rho family related to
            TC10 andCcdc42
  JOURNAL   J Biol Chem 275 (46), 36457-36464 (2000)
   PUBMED   10967094
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BM555132.1, AF087862.1, AK027351.1 and AL049871.4.
            
            On Nov 23, 2018 this sequence version replaced NM_020663.4.
            
            Summary: This gene encodes one of the many small GTP-binding
            proteins in the Rho family shown to be associated with focal
            adhesions in endothelial cells (PMID: 21148427, 22103495). The
            encoded protein is activated by vascular endothelial growth factor
            and may regulate angiogenesis. [provided by RefSeq, Dec 2011].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660809.82937.1,
                                           SRR1803612.5358.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000316754.8/ ENSP00000316729.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-73                BM555132.1         60-132
            74-74               AF087862.1         109-109
            75-1889             AK027351.1         93-1907
            1890-3556           AL049871.4         124805-126471
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q23.2"
     gene            1..3556
                     /gene="RHOJ"
                     /gene_synonym="ARHJ; RASL7B; TC10B; TCL"
                     /note="ras homolog family member J"
                     /db_xref="GeneID:57381"
                     /db_xref="HGNC:HGNC:688"
                     /db_xref="MIM:607653"
     exon            1..605
                     /gene="RHOJ"
                     /gene_synonym="ARHJ; RASL7B; TC10B; TCL"
                     /inference="alignment:Splign:2.1.0"
     CDS             428..1072
                     /gene="RHOJ"
                     /gene_synonym="ARHJ; RASL7B; TC10B; TCL"
                     /note="RAS-like, family 7, member B; TC10-like Rho GTPase;
                     tc10-like GTP-binding protein; ras-like protein family
                     member 7B; ras homolog gene family, member J"
                     /codon_start=1
                     /product="rho-related GTP-binding protein RhoJ"
                     /protein_id="NP_065714.1"
                     /db_xref="CCDS:CCDS9757.1"
                     /db_xref="GeneID:57381"
                     /db_xref="HGNC:HGNC:688"
                     /db_xref="MIM:607653"
                     /translation="MNCKEGTDSSCGCRGNDEKKMLKCVVVGDGAVGKTCLLMSYAND
                     AFPEEYVPTVFDHYAVTVTVGGKQHLLGLYDTAGQEDYNQLRPLSYPNTDVFLICFSV
                     VNPASYHNVQEEWVPELKDCMPHVPYVLIGTQIDLRDDPKTLARLLYMKEKPLTYEHG
                     VKLAKAIGAQCYLECSALTQKGLKAVFDEAILTIFHPKKKKKRCSEGHSCCSII"
     exon            606..664
                     /gene="RHOJ"
                     /gene_synonym="ARHJ; RASL7B; TC10B; TCL"
                     /inference="alignment:Splign:2.1.0"
     exon            665..829
                     /gene="RHOJ"
                     /gene_synonym="ARHJ; RASL7B; TC10B; TCL"
                     /inference="alignment:Splign:2.1.0"
     exon            830..925
                     /gene="RHOJ"
                     /gene_synonym="ARHJ; RASL7B; TC10B; TCL"
                     /inference="alignment:Splign:2.1.0"
     exon            926..3556
                     /gene="RHOJ"
                     /gene_synonym="ARHJ; RASL7B; TC10B; TCL"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3245..3250
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RHOJ"
                     /gene_synonym="ARHJ; RASL7B; TC10B; TCL"
                     /note="hexamer: AATAAA"
     polyA_site      3267
                     /gene="RHOJ"
                     /gene_synonym="ARHJ; RASL7B; TC10B; TCL"
                     /note="major polyA site"
     regulatory      3536..3541
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RHOJ"
                     /gene_synonym="ARHJ; RASL7B; TC10B; TCL"
                     /note="hexamer: AATAAA"
     polyA_site      3556
                     /gene="RHOJ"
                     /gene_synonym="ARHJ; RASL7B; TC10B; TCL"
ORIGIN      
        1 attaaaaagc ccagctttcc tccatgttag atgtgacttg gaaaatgaga aagatttagc
       61 aaaattccac cgtatctttt gccaggctag agacagggag agcagagtaa aaccctcagg
      121 ctgctgaaat ttctaggctg ttaggaagcc cctcgaattc tgtgaaaatg agggtttctt
      181 aactcacact gagagcggaa aggggcagac ccttttcata actccctcaa gtgtgtgtta
      241 cctttcttta ccagcatggt aagcaacagg acatatccca gcctcggaca tgtctgtatg
      301 atccaaggta cccaaagtca gacagagtaa actcaagcct ggcactggct ttctgccgct
      361 tcatgtgctt tggaaaaagc aggagaagca atagcagcag gagtccccag cagctggagc
      421 cgcaagaatg aactgcaaag agggaactga cagcagctgc ggctgcaggg gcaacgacga
      481 gaagaagatg ttgaagtgtg tggtggtggg ggacggtgcc gtggggaaaa cctgcctgct
      541 gatgagctac gccaacgacg ccttcccaga ggaatacgtg cccactgtgt ttgaccacta
      601 tgcagttact gtgactgtgg gaggcaagca acacttgctc ggactgtatg acaccgcggg
      661 acaggaggac tacaaccagc tgaggccact ctcctacccc aacacggatg tgtttttgat
      721 ctgcttctct gtcgtaaacc ctgcctctta ccacaatgtc caggaggaat gggtccccga
      781 gctcaaggac tgcatgcctc acgtgcctta tgtcctcata gggacccaga ttgatctccg
      841 tgatgaccca aaaaccttgg cccgtttgct gtatatgaaa gagaaacctc tcacttacga
      901 gcatggtgtg aagctcgcaa aagcgatcgg agcacagtgc tacttggaat gttcagctct
      961 gactcagaaa ggtctcaaag cggtttttga tgaagcaatc ctcaccattt tccaccccaa
     1021 gaaaaagaag aaacgctgtt ctgagggtca cagctgctgt tcaattatct gaggttgtct
     1081 gggacctgcc tccaccccat ccagggatga gaatggcagc caatctctgt ggccaagctc
     1141 cagccaaaaa ggagggcacg accagaaagg aactcccttt gcacggaggc ttgccccatc
     1201 accctctgag ccctcccaac acagcacact agtcagccca ctgccacgac ctccctgcca
     1261 gccagaagca tccgtactgc acgctgtctg agaatgctgg gcctggattg cagacagtgc
     1321 cgctgctgat cgcatcaaaa acaaagtcaa aggccatctc acattttaca aatccccagc
     1381 tcatgaacgt gaagctgata ggaaatcacc ccagggaacc cgaaaaagaa acttgattcc
     1441 tctattgctg gccttacttg atgtctttta taaaacttgg gactacaata ctaacctttt
     1501 tttctgaatc tgctgttcta cccatgtgtc tcacattcat ttgtattatt tcaagaaatg
     1561 tactaatttc cagttcactc aggccttact aatccatacc aaattagcct aaagacaagg
     1621 cattttatat tcatttctat tttcagcatg tttctaccaa agctattaga accaacacgt
     1681 acctctgaat gcccgattat aagaagacat gagaagactt taaaagtttt ggaaatttac
     1741 agagccatga tttttgaacc taattgaaag aaaaccatct gaattgttgc aggtccacat
     1801 ttttgccaaa gatacactct atagatgctt agtagtggcc tgattttttt ccatgtattg
     1861 ccacgacaaa ctaaaaatga actgtgttta agaatgtagt atttctgttt ttcatccaag
     1921 ttgattgggg gaagaatatg gcaggatcca tcttttacag tattttgtat tcagtaaagt
     1981 ggacattcct gctcctccct tcccccattg catgccctct tcctcccttg atttcacttt
     2041 ctctcatgcc cggatccttt tattctcccc agttataacc cagttataaa agaaagatct
     2101 gagcataaag atacgtgttt aaaaataact aaaagtaaag gaaagtgcct taatttttct
     2161 atttgcttca actgaaagtg cttctcagct cgccccatgt aagttctcat tccatgtaaa
     2221 tgacattttc cagttacaac tggtactgag attttgcctc tctctttcct tactcatcct
     2281 cccaaatgtc tttgtgggag ccatatcagt ggataccaag ctctgtatcc atttgtcccc
     2341 tgccctccac aatgtgtgac atagaacagg gactttggcc ctgggaaagc aaaagctccc
     2401 agtaaggaat cctgtgccca atgatgtaaa acaattccaa acatccagga atttttgtat
     2461 catagagcga attacttcct atcttttcat tagaggctat gaggacttct aattagtctt
     2521 agttgcttat aagtgccctg gaatcaccca ggtaggcact taattttttt ttcagttgca
     2581 tgagcaaagt gcttcttagt agtgtgaaat tacaacaact ttaagacttt ccagattcaa
     2641 gctcccactg ttggaaaaag ccagcctttc taatctcttc tgctactgga ataagcactt
     2701 aagaattgcg tgatagccag gcaccgtggc tcatgcctgt aatcccaaca cttagggagg
     2761 ctgaggtggg tgggccgctt gagctcagga gttcaagacc agcctgggta atatagtgag
     2821 atcctgtgtc tctataaaaa aattaaaaat tagtcagttg tagtgacaca tacctgtagt
     2881 cccagctact caggaggctg aggtggaagg atcacttgag cccagaaggt aaggctgcag
     2941 tgagctgtga ctgtgccact acactccagc ctgagtgaca gagaaagaac ctgtcaaaaa
     3001 aaaaaaaaaa acaacctaca tttcaagtac tatttccctt ctctcccatc taattgctaa
     3061 agattttctt tcatacgcac acactccagt gactggaaaa acgggagttt tcagtcaaag
     3121 cttgacattt agagaaaaca aggactttct gcctttataa atggaaatca actgtgtatg
     3181 aactataact ctgcagaggt tatgaattca tcctttacaa acaataatga acttttagtc
     3241 ctgtaataaa tgaaatgtta ttaggcagct ttgttgcatg attgcatagt tatatcttgc
     3301 taacgggcca ctcatttctc actgatgtgg atgaaaaaat gagagcagta tgtttccagg
     3361 tgtgtgcact caacaggcaa atagctcccg aggtcaccac ttccctaatg ggccacagga
     3421 agtaagttga tcttgatggg gagatcacgt cacccagaac cagcaactgg atagagactg
     3481 ttgttagtgt ctgggtagag cacaggctcc caggggtctt aagagctaat tactgaataa
     3541 aacaatctag aacaaa
//
