LOCUS       NM_005614               2046 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens Ras homolog, mTORC1 binding (RHEB), mRNA.
ACCESSION   NM_005614
VERSION     NM_005614.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Hutchins,C.M. and Gorfe,A.A.
  TITLE     Intrinsically Disordered Membrane Anchors of Rheb, RhoA, and DiRas3
            Small GTPases: Molecular Dynamics, Membrane Organization, and
            Interactions
  JOURNAL   J Phys Chem B 128 (27), 6518-6528 (2024)
   PUBMED   38942776
  REMARK    GeneRIF: Intrinsically Disordered Membrane Anchors of Rheb, RhoA,
            and DiRas3 Small GTPases: Molecular Dynamics, Membrane
            Organization, and Interactions.
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Rahman,M., Nguyen,T.M., Lee,G.J., Kim,B., Park,M.K. and Lee,C.H.
  TITLE     Unraveling the Role of Ras Homolog Enriched in Brain (Rheb1 and
            Rheb2): Bridging Neuronal Dynamics and Cancer Pathogenesis through
            Mechanistic Target of Rapamycin Signaling
  JOURNAL   Int J Mol Sci 25 (3), 1489 (2024)
   PUBMED   38338768
  REMARK    GeneRIF: Unraveling the Role of Ras Homolog Enriched in Brain
            (Rheb1 and Rheb2): Bridging Neuronal Dynamics and Cancer
            Pathogenesis through Mechanistic Target of Rapamycin Signaling.
            Review article
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2046)
  AUTHORS   Fu,W. and Wu,G.
  TITLE     Design of negative-regulating proteins of Rheb/mTORC1 with
            much-reduced sizes of the tuberous sclerosis protein complex
  JOURNAL   Protein Sci 32 (8), e4731 (2023)
   PUBMED   37462942
  REMARK    GeneRIF: Design of negative-regulating proteins of Rheb/mTORC1 with
            much-reduced sizes of the tuberous sclerosis protein complex.
REFERENCE   4  (bases 1 to 2046)
  AUTHORS   Pham,L.T., Peng,H., Ueno,M., Kohno,S., Kasada,A., Hosomichi,K.,
            Sato,T., Kurayoshi,K., Kobayashi,M., Tadokoro,Y., Kasahara,A.,
            Shoulkamy,M.I., Xiao,B., Worley,P.F., Takahashi,C., Tajima,A. and
            Hirao,A.
  TITLE     RHEB is a potential therapeutic target in T cell acute
            lymphoblastic leukemia
  JOURNAL   Biochem Biophys Res Commun 621, 74-79 (2022)
   PUBMED   35810594
  REMARK    GeneRIF: RHEB is a potential therapeutic target in T cell acute
            lymphoblastic leukemia.
REFERENCE   5  (bases 1 to 2046)
  AUTHORS   Liu,X., Wang,X., Chai,B., Wu,Z., Gu,Z., Zou,H., Zhang,H., Li,Y.,
            Sun,Q., Fang,W. and Ma,Z.
  TITLE     miR-199a-3p/5p regulate tumorgenesis via targeting Rheb in
            non-small cell lung cancer
  JOURNAL   Int J Biol Sci 18 (10), 4187-4202 (2022)
   PUBMED   35844793
  REMARK    GeneRIF: miR-199a-3p/5p regulate tumorgenesis via targeting Rheb in
            non-small cell lung cancer.
            Erratum:[Int J Biol Sci. 2023 Jul 27;19(12):3830. doi:
            10.7150/ijbs.86344.. PMID: 37564201]
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2046)
  AUTHORS   Clark,G.J., Kinch,M.S., Rogers-Graham,K., Sebti,S.M., Hamilton,A.D.
            and Der,C.J.
  TITLE     The Ras-related protein Rheb is farnesylated and antagonizes Ras
            signaling and transformation
  JOURNAL   J Biol Chem 272 (16), 10608-10615 (1997)
   PUBMED   9099708
REFERENCE   7  (bases 1 to 2046)
  AUTHORS   Yee,W.M. and Worley,P.F.
  TITLE     Rheb interacts with Raf-1 kinase and may function to integrate
            growth factor- and protein kinase A-dependent signals
  JOURNAL   Mol Cell Biol 17 (2), 921-933 (1997)
   PUBMED   9001246
REFERENCE   8  (bases 1 to 2046)
  AUTHORS   Mizuki,N., Kimura,M., Ohno,S., Miyata,S., Sato,M., Ando,H.,
            Ishihara,M., Goto,K., Watanabe,S., Yamazaki,M., Ono,A., Taguchi,S.,
            Okumura,K., Nogami,M., Taguchi,T., Ando,A. and Inoko,H.
  TITLE     Isolation of cDNA and genomic clones of a human Ras-related
            GTP-binding protein gene and its chromosomal localization to the
            long arm of chromosome 7, 7q36
  JOURNAL   Genomics 34 (1), 114-118 (1996)
   PUBMED   8661031
REFERENCE   9  (bases 1 to 2046)
  AUTHORS   Gromov,P.S., Madsen,P., Tomerup,N. and Celis,J.E.
  TITLE     A novel approach for expression cloning of small GTPases:
            identification, tissue distribution and chromosome mapping of the
            human homolog of rheb
  JOURNAL   FEBS Lett 377 (2), 221-226 (1995)
   PUBMED   8543055
REFERENCE   10 (bases 1 to 2046)
  AUTHORS   Yamagata,K., Sanders,L.K., Kaufmann,W.E., Yee,W., Barnes,C.A.,
            Nathans,D. and Worley,P.F.
  TITLE     rheb, a growth factor- and synaptic activity-regulated gene,
            encodes a novel Ras-related protein
  JOURNAL   J Biol Chem 269 (23), 16333-16339 (1994)
   PUBMED   8206940
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BG702213.1, BC016155.1,
            Z29677.1, DA475169.1 and BM670621.1.
            
            On Nov 22, 2018 this sequence version replaced NM_005614.3.
            
            Summary: This gene is a member of the small GTPase superfamily and
            encodes a lipid-anchored, cell membrane protein with five repeats
            of the RAS-related GTP-binding region. This protein is vital in
            regulation of growth and cell cycle progression due to its role in
            the insulin/TOR/S6K signaling pathway. The protein has GTPase
            activity and shuttles between a GDP-bound form and a GTP-bound
            form, and farnesylation of the protein is required for this
            activity. Three pseudogenes have been mapped, two on chromosome 10
            and one on chromosome 22. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK125446.1, BC016155.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000262187.10/ ENSP00000262187.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-70                BG702213.1         35-104
            71-361              BC016155.1         100-390
            362-1348            Z29677.1           1-987
            1349-1596           DA475169.1         317-564
            1597-2046           BM670621.1         18-467              c
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q36.1"
     gene            1..2046
                     /gene="RHEB"
                     /gene_synonym="RHEB2"
                     /note="Ras homolog, mTORC1 binding"
                     /db_xref="GeneID:6009"
                     /db_xref="HGNC:HGNC:10011"
                     /db_xref="MIM:601293"
     exon            1..436
                     /gene="RHEB"
                     /gene_synonym="RHEB2"
                     /inference="alignment:Splign:2.1.0"
     CDS             385..939
                     /gene="RHEB"
                     /gene_synonym="RHEB2"
                     /EC_number="3.6.5.2"
                     /note="Ras homolog enriched in brain 2"
                     /codon_start=1
                     /product="GTP-binding protein Rheb"
                     /protein_id="NP_005605.1"
                     /db_xref="CCDS:CCDS5927.1"
                     /db_xref="GeneID:6009"
                     /db_xref="HGNC:HGNC:10011"
                     /db_xref="MIM:601293"
                     /translation="MPQSKSRKIAILGYRSVGKSSLTIQFVEGQFVDSYDPTIENTFT
                     KLITVNGQEYHLQLVDTAGQDEYSIFPQTYSIDINGYILVYSVTSIKSFEVIKVIHGK
                     LLDMVGKVQIPIMLVGNKKDLHMERVISYEEGKALAESWNAAFLESSAKENQTAVDVF
                     RRIILEAEKMDGAASQGKSSCSVM"
     exon            437..508
                     /gene="RHEB"
                     /gene_synonym="RHEB2"
                     /inference="alignment:Splign:2.1.0"
     exon            509..576
                     /gene="RHEB"
                     /gene_synonym="RHEB2"
                     /inference="alignment:Splign:2.1.0"
     exon            577..659
                     /gene="RHEB"
                     /gene_synonym="RHEB2"
                     /inference="alignment:Splign:2.1.0"
     exon            660..716
                     /gene="RHEB"
                     /gene_synonym="RHEB2"
                     /inference="alignment:Splign:2.1.0"
     exon            717..764
                     /gene="RHEB"
                     /gene_synonym="RHEB2"
                     /inference="alignment:Splign:2.1.0"
     exon            765..846
                     /gene="RHEB"
                     /gene_synonym="RHEB2"
                     /inference="alignment:Splign:2.1.0"
     exon            847..2046
                     /gene="RHEB"
                     /gene_synonym="RHEB2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1328..1333
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RHEB"
                     /gene_synonym="RHEB2"
                     /note="hexamer: AATAAA"
     polyA_site      1349
                     /gene="RHEB"
                     /gene_synonym="RHEB2"
                     /note="major polyA site"
     regulatory      2023..2028
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RHEB"
                     /gene_synonym="RHEB2"
                     /note="hexamer: AATAAA"
     polyA_site      2046
                     /gene="RHEB"
                     /gene_synonym="RHEB2"
                     /note="major polyA site"
ORIGIN      
        1 gggagggtca tgacgcagcg agtttcagtc gtgacttttc tgggggcatc gcggcgtccc
       61 ctttttttgc ctttaaagta aaacgtcgcc ccgacgcacc ccccgcgtat ttcggggggc
      121 ggaggcggcg ggccacggcg cgaagagggg cggtgctgac gccggccggt cacgtgggcg
      181 tgttgtgggg gggaggggcg ccgccgcgcg gtcggttccg ggcggttggg agcgcgcgag
      241 ctagcgagcg agaggcagcc gcgcccgccg ccgcccctgc tctgtatgcc gctctctccc
      301 ggcgcggccg ccgccgatca cagcagcagg agccaccgcc gccgcggttg atgtggttgg
      361 gccggggctg aggaggccgc caagatgccg cagtccaagt cccggaagat cgcgatcctg
      421 ggctaccggt ctgtggggaa atcctcattg acgattcaat ttgttgaagg ccaatttgtg
      481 gactcctacg atccaaccat agaaaacact tttacaaagt tgatcacagt aaatggacaa
      541 gaatatcatc ttcaacttgt agacacagcc gggcaagatg aatattctat ctttcctcag
      601 acatactcca tagatattaa tggctatatt cttgtgtatt ctgttacatc aatcaaaagt
      661 tttgaagtga ttaaagttat ccatggcaaa ttgttggata tggtggggaa agtacaaata
      721 cctattatgt tggttgggaa taagaaagac ctgcatatgg aaagggtgat cagttatgaa
      781 gaagggaaag ctttggcaga atcttggaat gcagcttttt tggaatcttc tgctaaagaa
      841 aatcagactg ctgtggatgt ttttcgaagg ataattttgg aggcagaaaa aatggacggg
      901 gcagcttcac aaggcaagtc ttcatgctcg gtgatgtgat tctgctgcaa agcctgagga
      961 cactgggaat atattctacc tgaagaagca aactgcccgt tctccttgaa gataaactat
     1021 gcttcttttt tcttctgtta acctgaaaga tatcatttgg gtcagagctc ccctcccttc
     1081 agattatgtt aactctgagt ctgtccaaat gagttcactt ccattttcaa attttaagca
     1141 atcatatttt caatttatat attgtatttc ttaatattat gaccaagaat tttatcggca
     1201 ttaatttttc agtgtagttt gttgtttaaa ataatgtaat catcaaaatg atgcatattg
     1261 ttacactact attaactagg cttcagtata tcagtgttta tttcattgtg ttaaatgtat
     1321 acttgtaaat aaaatagctg caaacctcag tcctttgtgc tacttgatgt ggctttcaaa
     1381 gaagagaagc cttgtcctga gtttctcact tggcttcagg aaggccccag gttggattcc
     1441 agaaaccagt gaagatgtgg ccacaggagg aggtgtgctg aggtggctgc tgaccgtgga
     1501 ctccctgcgc agtggcctgc agatgttggg gctgggttac agctgattga agctgagtgg
     1561 ccctgggggg tctgtgaggg gagttcctcc ccagtgatga aattctctcc ttccaccctc
     1621 aaatccctag accttgactg aaatgctccg tggtcgggag cctggtcaag gaggaggagc
     1681 tgctgagagg cattgttcgc ccttgctcat agcttagctc gatgtccgtg tcagacagga
     1741 gatgattgag aacagccttg cctgtcactg tcctagaaca ccctggagtt tagtgttctg
     1801 tgtcagagtc ttgggagcct ccttcagacc cagatgacgg gcctccctct gtccaaggag
     1861 cagctgtaaa ggagaagagg gatttcattt gtttggtggc tgttaccttg tctgtaagtc
     1921 aaacttggag ttgagcagtg ctttttaaac gattcccttt tgcagctaaa atttcacagg
     1981 gctatttcta atacgtaagc aaatgttacc attgacttta ttaataaaat atagttttgc
     2041 tttgca
//
