LOCUS       NM_020407               1790 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens Rh family B glycoprotein (RHBG), transcript variant 1,
            mRNA.
ACCESSION   NM_020407
VERSION     NM_020407.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Mishra,S., Welch,N., Singh,S.S., Singh,K.D., Bellar,A., Kumar,A.,
            Deutz,L.N., Hanlon,M.D., Kant,S., Dastidar,S., Patel,H.,
            Agrawal,V., Attaway,A.H., Musich,R., Stark,G.R., Tedesco,F.S.,
            Truskey,G.A., Weiner,I.D., Karnik,S.S. and Dasarathy,S.
  TITLE     Ammonia transporter RhBG initiates downstream signaling and
            functional responses by activating NFkappaB
  JOURNAL   Proc Natl Acad Sci U S A 121 (31), e2314760121 (2024)
   PUBMED   39052834
  REMARK    GeneRIF: Ammonia transporter RhBG initiates downstream signaling
            and functional responses by activating NFkappaB.
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 1790)
  AUTHORS   Sohet,F., Colin,Y., Genetet,S., Ripoche,P., Metral,S., Le Van
            Kim,C. and Lopez,C.
  TITLE     Phosphorylation and ankyrin-G binding of the C-terminal domain
            regulate targeting and function of the ammonium transporter RhBG
  JOURNAL   J Biol Chem 283 (39), 26557-26567 (2008)
   PUBMED   18635543
  REMARK    GeneRIF: Phosphorylation and ankyrin-G binding of the C-terminal
            domain regulate targeting and function of the ammonium transporter
            RhBG
REFERENCE   4  (bases 1 to 1790)
  AUTHORS   Huang,C.H. and Peng,J.
  TITLE     Evolutionary conservation and diversification of Rh family genes
            and proteins
  JOURNAL   Proc Natl Acad Sci U S A 102 (43), 15512-15517 (2005)
   PUBMED   16227429
REFERENCE   5  (bases 1 to 1790)
  AUTHORS   Zidi-Yahiaoui,N., Mouro-Chanteloup,I., D'Ambrosio,A.M., Lopez,C.,
            Gane,P., Le van Kim,C., Cartron,J.P., Colin,Y. and Ripoche,P.
  TITLE     Human Rhesus B and Rhesus C glycoproteins: properties of
            facilitated ammonium transport in recombinant kidney cells
  JOURNAL   Biochem J 391 (Pt 1), 33-40 (2005)
   PUBMED   15929723
  REMARK    GeneRIF: RhBG and RhCG facilitate ammonium movement across the
            plasma membrane; these results favor the hypothesis that these Rh
            glycoproteins, together with their homolog RhAG, constitute a
            family of NH3 channels in mammalian cells.
REFERENCE   6  (bases 1 to 1790)
  AUTHORS   Lopez,C., Metral,S., Eladari,D., Drevensek,S., Gane,P.,
            Chambrey,R., Bennett,V., Cartron,J.P., Le Van Kim,C. and Colin,Y.
  TITLE     The ammonium transporter RhBG: requirement of a tyrosine-based
            signal and ankyrin-G for basolateral targeting and membrane
            anchorage in polarized kidney epithelial cells
  JOURNAL   J Biol Chem 280 (9), 8221-8228 (2005)
   PUBMED   15611082
  REMARK    GeneRIF: RhBG requires a tyrosine-based signal and ankyrin-G for
            basolateral targeting and membrane
REFERENCE   7  (bases 1 to 1790)
  AUTHORS   Quentin,F., Eladari,D., Cheval,L., Lopez,C., Goossens,D., Colin,Y.,
            Cartron,J.P., Paillard,M. and Chambrey,R.
  TITLE     RhBG and RhCG, the putative ammonia transporters, are expressed in
            the same cells in the distal nephron
  JOURNAL   J Am Soc Nephrol 14 (3), 545-554 (2003)
   PUBMED   12595489
REFERENCE   8  (bases 1 to 1790)
  AUTHORS   Liu,Z., Peng,J., Mo,R., Hui,C. and Huang,C.H.
  TITLE     Rh type B glycoprotein is a new member of the Rh superfamily and a
            putative ammonia transporter in mammals
  JOURNAL   J Biol Chem 276 (2), 1424-1433 (2001)
   PUBMED   11024028
REFERENCE   9  (bases 1 to 1790)
  AUTHORS   Huang,C.H. and Liu,P.Z.
  TITLE     New insights into the Rh superfamily of genes and proteins in
            erythroid cells and nonerythroid tissues
  JOURNAL   Blood Cells Mol Dis 27 (1), 90-101 (2001)
   PUBMED   11358367
  REMARK    Review article
REFERENCE   10 (bases 1 to 1790)
  AUTHORS   Liu,Z., Chen,Y., Mo,R., Hui,C., Cheng,J.F., Mohandas,N. and
            Huang,C.H.
  TITLE     Characterization of human RhCG and mouse Rhcg as novel nonerythroid
            Rh glycoprotein homologues predominantly expressed in kidney and
            testis
  JOURNAL   J Biol Chem 275 (33), 25641-25651 (2000)
   PUBMED   10852913
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL589685.17 and AF193807.1.
            
            On Nov 20, 2018 this sequence version replaced NM_020407.4.
            
            Summary: This gene encodes one of two non-erythroid members of the
            Rhesus (Rh) protein family. Non-erythroid Rh protein family members
            are mainly expressed in the kidney and belong to the
            methylammonium-ammonium permease/ammonia transporters superfamily.
            All Rh family proteins are predicted to be transmembrane proteins
            with 12 membrane spanning domains and intracytoplasmic N- and
            C-termini. Alternative splicing of this gene results in multiple
            transcript variants. [provided by RefSeq, Jan 2012].
            
            Transcript Variant: This variant (1) uses the same exon combination
            as variant 8 but represents the allele encoded by the GRCh38
            reference genome. The encoded isoform (a) has a frameshifted
            C-terminus compared to isoform d.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF193807.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000537040.6/ ENSP00000441197.2
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1                 AL589685.17        94731-94731
            2-1790              AF193807.1         1-1789
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q22"
     gene            1..1790
                     /gene="RHBG"
                     /gene_synonym="SLC42A2"
                     /note="Rh family B glycoprotein"
                     /db_xref="GeneID:57127"
                     /db_xref="HGNC:HGNC:14572"
                     /db_xref="MIM:607079"
     exon            1..226
                     /gene="RHBG"
                     /gene_synonym="SLC42A2"
                     /inference="alignment:Splign:2.1.0"
     CDS             40..1416
                     /gene="RHBG"
                     /gene_synonym="SLC42A2"
                     /note="isoform a is encoded by transcript variant 1;
                     Rhesus blood group, B glycoprotein; ammonium transporter
                     Rh type B; solute carrier family 42 member 2"
                     /codon_start=1
                     /product="ammonium transporter Rh type B isoform a"
                     /protein_id="NP_065140.3"
                     /db_xref="CCDS:CCDS41414.2"
                     /db_xref="GeneID:57127"
                     /db_xref="HGNC:HGNC:14572"
                     /db_xref="MIM:607079"
                     /translation="MAGSPSRAAGRRLQLPLLCLFLQGATAVLFAVFVRYNHKTDAAL
                     WHRSNHSNADNEFYFRYPSFQDVHAMVFVGFGFLMVFLQRYGFSSVGFTFLLAAFALQ
                     WSTLVQGFLHSFHGGHIHVGVESMINADFCAGAVLISFGAVLGKTGPTQLLLMALLEV
                     VLFGINEFVLLHLLGVRDAGGSMTIHTFGAYFGLVLSRVLYRPQLEKSKHRQGSVYHS
                     DLFAMIGTIFLWIFWPSFNAALTALGAGQHRTALNTYYSLAASTLGTFALSALVGEDG
                     RLDMVHIQNAALAGGVVVGTSSEMMLTPFGALAAGFLAGTVSTLGYKFFTPILESKFK
                     VQDTCGVHNLHGMPGVLGALLGVLVAGLATHEAYGDGLESVFPLIAEGQRSATSQAMH
                     QLFGLFVTLMFASVGGGLGGLLLKLPFLDSPPDSQHYEDQVHWQVPGEHEDKAQRPLR
                     VEEADTQA"
     exon            227..413
                     /gene="RHBG"
                     /gene_synonym="SLC42A2"
                     /inference="alignment:Splign:2.1.0"
     exon            414..564
                     /gene="RHBG"
                     /gene_synonym="SLC42A2"
                     /inference="alignment:Splign:2.1.0"
     exon            565..712
                     /gene="RHBG"
                     /gene_synonym="SLC42A2"
                     /inference="alignment:Splign:2.1.0"
     exon            713..879
                     /gene="RHBG"
                     /gene_synonym="SLC42A2"
                     /inference="alignment:Splign:2.1.0"
     exon            880..1017
                     /gene="RHBG"
                     /gene_synonym="SLC42A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1018..1151
                     /gene="RHBG"
                     /gene_synonym="SLC42A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1152..1273
                     /gene="RHBG"
                     /gene_synonym="SLC42A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1274..1347
                     /gene="RHBG"
                     /gene_synonym="SLC42A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1348..1790
                     /gene="RHBG"
                     /gene_synonym="SLC42A2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1770..1775
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RHBG"
                     /gene_synonym="SLC42A2"
                     /note="hexamer: GATAAA"
     polyA_site      1790
                     /gene="RHBG"
                     /gene_synonym="SLC42A2"
                     /note="major polyA site"
ORIGIN      
        1 caaagcctgc gagcgccagc cgagatcgca gcccaaccca tggccgggtc tcctagccgc
       61 gccgcgggcc ggcgactgca gcttcccctg ctgtgcctct tcctccaggg cgccactgcc
      121 gtcctctttg ctgtctttgt ccgctacaac cacaaaaccg acgctgccct ctggcaccgg
      181 agcaaccaca gtaacgcgga caatgaattt tactttcgct acccaagctt ccaggacgtg
      241 catgccatgg tcttcgtggg ctttggcttc ctcatggtct tcctgcagcg ttacggcttc
      301 agcagcgtgg gcttcacctt cctcctggcc gcctttgccc tgcagtggtc cacactggtc
      361 cagggctttc tccactcctt ccacggtggc cacatccatg ttggcgtgga gagcatgatc
      421 aatgctgact tttgtgcggg ggccgtgctc atctcctttg gtgccgtcct gggcaagacc
      481 gggcctaccc agctgctgct catggccctg ctggaggtgg tgctgtttgg catcaatgag
      541 tttgtgctcc ttcatctcct gggggtgaga gatgccggag gctccatgac tatccacacc
      601 tttggtgcct acttcgggct cgtcctttcg cgggttctgt acaggcccca gctggagaag
      661 agcaagcacc gccagggctc cgtctaccat tcagacctct tcgccatgat tgggaccatc
      721 ttcctgtgga tcttctggcc tagcttcaat gctgcactca cagcgctggg ggctgggcag
      781 catcggacgg ccctcaacac atactactcc ctggctgcca gcacccttgg cacctttgcc
      841 ttgtcagccc ttgtagggga agatgggagg cttgacatgg tccacatcca aaatgcagcg
      901 ctggctggag gggttgtggt ggggacctca agtgaaatga tgctgacacc ctttggggct
      961 ctggcagctg gcttcttggc tgggactgtc tccacgctgg ggtacaagtt cttcacgccc
     1021 atccttgaat caaaattcaa agtccaagac acatgtggag tccacaacct ccatgggatg
     1081 ccgggggtcc tgggggccct cctgggggtc cttgtggctg gacttgccac ccatgaagct
     1141 tacggagatg gcctggagag tgtgtttcca ctcatagccg agggccagcg cagtgccacg
     1201 tcacaggcca tgcaccagct cttcgggctg tttgtcacac tgatgtttgc ctctgtgggc
     1261 gggggccttg gagggctcct gctgaagcta ccctttctgg actccccccc agactcccag
     1321 cactacgagg accaagttca ctggcaggtg cctggcgagc atgaggataa agcccagaga
     1381 cctctgaggg tggaggaggc agacactcag gcctaaccca ctgccagccc ctgagaggac
     1441 acgctccttt tcgaagatgc tgactggctg ctactaggaa gttctttttg agctcccatt
     1501 cctccagctg caagaaggga gccatgagcc agaaggaggc ccctttccac aggcagcgtc
     1561 tccacaggga gaggggcaac aggaggctgg gaaatggtgg ggagtggggc cgtaactggg
     1621 tacaataggg ggaacctcac cagatgccca acccgactgc cctaccagcc tgcacatggg
     1681 tagaagaggc caaattgagg cacccaagtg atccactggc cccacgtcac acagttacag
     1741 tgaagcccaa gccaggcctg gttgagggtg ataaacgcca ctgtctctaa
//
