LOCUS       NM_001256396            1863 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens Rh family B glycoprotein (RHBG), transcript variant 3,
            mRNA.
ACCESSION   NM_001256396
VERSION     NM_001256396.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Mishra,S., Welch,N., Singh,S.S., Singh,K.D., Bellar,A., Kumar,A.,
            Deutz,L.N., Hanlon,M.D., Kant,S., Dastidar,S., Patel,H.,
            Agrawal,V., Attaway,A.H., Musich,R., Stark,G.R., Tedesco,F.S.,
            Truskey,G.A., Weiner,I.D., Karnik,S.S. and Dasarathy,S.
  TITLE     Ammonia transporter RhBG initiates downstream signaling and
            functional responses by activating NFkappaB
  JOURNAL   Proc Natl Acad Sci U S A 121 (31), e2314760121 (2024)
   PUBMED   39052834
  REMARK    GeneRIF: Ammonia transporter RhBG initiates downstream signaling
            and functional responses by activating NFkappaB.
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 1863)
  AUTHORS   Sohet,F., Colin,Y., Genetet,S., Ripoche,P., Metral,S., Le Van
            Kim,C. and Lopez,C.
  TITLE     Phosphorylation and ankyrin-G binding of the C-terminal domain
            regulate targeting and function of the ammonium transporter RhBG
  JOURNAL   J Biol Chem 283 (39), 26557-26567 (2008)
   PUBMED   18635543
  REMARK    GeneRIF: Phosphorylation and ankyrin-G binding of the C-terminal
            domain regulate targeting and function of the ammonium transporter
            RhBG
REFERENCE   4  (bases 1 to 1863)
  AUTHORS   Huang,C.H. and Peng,J.
  TITLE     Evolutionary conservation and diversification of Rh family genes
            and proteins
  JOURNAL   Proc Natl Acad Sci U S A 102 (43), 15512-15517 (2005)
   PUBMED   16227429
REFERENCE   5  (bases 1 to 1863)
  AUTHORS   Zidi-Yahiaoui,N., Mouro-Chanteloup,I., D'Ambrosio,A.M., Lopez,C.,
            Gane,P., Le van Kim,C., Cartron,J.P., Colin,Y. and Ripoche,P.
  TITLE     Human Rhesus B and Rhesus C glycoproteins: properties of
            facilitated ammonium transport in recombinant kidney cells
  JOURNAL   Biochem J 391 (Pt 1), 33-40 (2005)
   PUBMED   15929723
  REMARK    GeneRIF: RhBG and RhCG facilitate ammonium movement across the
            plasma membrane; these results favor the hypothesis that these Rh
            glycoproteins, together with their homolog RhAG, constitute a
            family of NH3 channels in mammalian cells.
REFERENCE   6  (bases 1 to 1863)
  AUTHORS   Lopez,C., Metral,S., Eladari,D., Drevensek,S., Gane,P.,
            Chambrey,R., Bennett,V., Cartron,J.P., Le Van Kim,C. and Colin,Y.
  TITLE     The ammonium transporter RhBG: requirement of a tyrosine-based
            signal and ankyrin-G for basolateral targeting and membrane
            anchorage in polarized kidney epithelial cells
  JOURNAL   J Biol Chem 280 (9), 8221-8228 (2005)
   PUBMED   15611082
  REMARK    GeneRIF: RhBG requires a tyrosine-based signal and ankyrin-G for
            basolateral targeting and membrane
REFERENCE   7  (bases 1 to 1863)
  AUTHORS   Quentin,F., Eladari,D., Cheval,L., Lopez,C., Goossens,D., Colin,Y.,
            Cartron,J.P., Paillard,M. and Chambrey,R.
  TITLE     RhBG and RhCG, the putative ammonia transporters, are expressed in
            the same cells in the distal nephron
  JOURNAL   J Am Soc Nephrol 14 (3), 545-554 (2003)
   PUBMED   12595489
REFERENCE   8  (bases 1 to 1863)
  AUTHORS   Liu,Z., Peng,J., Mo,R., Hui,C. and Huang,C.H.
  TITLE     Rh type B glycoprotein is a new member of the Rh superfamily and a
            putative ammonia transporter in mammals
  JOURNAL   J Biol Chem 276 (2), 1424-1433 (2001)
   PUBMED   11024028
REFERENCE   9  (bases 1 to 1863)
  AUTHORS   Huang,C.H. and Liu,P.Z.
  TITLE     New insights into the Rh superfamily of genes and proteins in
            erythroid cells and nonerythroid tissues
  JOURNAL   Blood Cells Mol Dis 27 (1), 90-101 (2001)
   PUBMED   11358367
  REMARK    Review article
REFERENCE   10 (bases 1 to 1863)
  AUTHORS   Liu,Z., Chen,Y., Mo,R., Hui,C., Cheng,J.F., Mohandas,N. and
            Huang,C.H.
  TITLE     Characterization of human RhCG and mouse Rhcg as novel nonerythroid
            Rh glycoprotein homologues predominantly expressed in kidney and
            testis
  JOURNAL   J Biol Chem 275 (33), 25641-25651 (2000)
   PUBMED   10852913
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL589685.17 and AY139093.1.
            
            On Nov 20, 2018 this sequence version replaced NM_001256396.1.
            
            Summary: This gene encodes one of two non-erythroid members of the
            Rhesus (Rh) protein family. Non-erythroid Rh protein family members
            are mainly expressed in the kidney and belong to the
            methylammonium-ammonium permease/ammonia transporters superfamily.
            All Rh family proteins are predicted to be transmembrane proteins
            with 12 membrane spanning domains and intracytoplasmic N- and
            C-termini. Alternative splicing of this gene results in multiple
            transcript variants. [provided by RefSeq, Jan 2012].
            
            Transcript Variant: This variant (3) uses the same exon combination
            as variant 10 but represents the allele encoded by the GRCh38
            reference genome. The encoded isoform (c) has a frameshifted
            C-terminus compared to isoform f.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY139093.1 [ECO:0000332]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1                 AL589685.17        94731-94731
            2-1863              AY139093.1         1-1862
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q22"
     gene            1..1863
                     /gene="RHBG"
                     /gene_synonym="SLC42A2"
                     /note="Rh family B glycoprotein"
                     /db_xref="GeneID:57127"
                     /db_xref="HGNC:HGNC:14572"
                     /db_xref="MIM:607079"
     exon            1..226
                     /gene="RHBG"
                     /gene_synonym="SLC42A2"
                     /inference="alignment:Splign:2.1.0"
     CDS             203..1489
                     /gene="RHBG"
                     /gene_synonym="SLC42A2"
                     /note="isoform c is encoded by transcript variant 3;
                     Rhesus blood group, B glycoprotein; ammonium transporter
                     Rh type B; solute carrier family 42 member 2"
                     /codon_start=1
                     /product="ammonium transporter Rh type B isoform c"
                     /protein_id="NP_001243325.1"
                     /db_xref="GeneID:57127"
                     /db_xref="HGNC:HGNC:14572"
                     /db_xref="MIM:607079"
                     /translation="MNFTFATQKSLTLLPRLECNGAISAHCNLHLPGFQDVHAMVFVG
                     FGFLMVFLQRYGFSSVGFTFLLAAFALQWSTLVQGFLHSFHGGHIHVGVESMINADFC
                     AGAVLISFGAVLGKTGPTQLLLMALLEVVLFGINEFVLLHLLGVRDAGGSMTIHTFGA
                     YFGLVLSRVLYRPQLEKSKHRQGSVYHSDLFAMIGTIFLWIFWPSFNAALTALGAGQH
                     RTALNTYYSLAASTLGTFALSALVGEDGRLDMVHIQNAALAGGVVVGTSSEMMLTPFG
                     ALAAGFLAGTVSTLGYKFFTPILESKFKVQDTCGVHNLHGMPGVLGALLGVLVAGLAT
                     HEAYGDGLESVFPLIAEGQRSATSQAMHQLFGLFVTLMFASVGGGLGGLLLKLPFLDS
                     PPDSQHYEDQVHWQVPGEHEDKAQRPLRVEEADTQA"
     exon            227..299
                     /gene="RHBG"
                     /gene_synonym="SLC42A2"
                     /inference="alignment:Splign:2.1.0"
     exon            300..486
                     /gene="RHBG"
                     /gene_synonym="SLC42A2"
                     /inference="alignment:Splign:2.1.0"
     exon            487..637
                     /gene="RHBG"
                     /gene_synonym="SLC42A2"
                     /inference="alignment:Splign:2.1.0"
     exon            638..785
                     /gene="RHBG"
                     /gene_synonym="SLC42A2"
                     /inference="alignment:Splign:2.1.0"
     exon            786..952
                     /gene="RHBG"
                     /gene_synonym="SLC42A2"
                     /inference="alignment:Splign:2.1.0"
     exon            953..1090
                     /gene="RHBG"
                     /gene_synonym="SLC42A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1091..1224
                     /gene="RHBG"
                     /gene_synonym="SLC42A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1225..1346
                     /gene="RHBG"
                     /gene_synonym="SLC42A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1347..1420
                     /gene="RHBG"
                     /gene_synonym="SLC42A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1421..1863
                     /gene="RHBG"
                     /gene_synonym="SLC42A2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1843..1848
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RHBG"
                     /gene_synonym="SLC42A2"
                     /note="hexamer: GATAAA"
     polyA_site      1863
                     /gene="RHBG"
                     /gene_synonym="SLC42A2"
                     /note="major polyA site"
ORIGIN      
        1 caaagcctgc gagcgccagc cgagatcgca gcccaaccca tggccgggtc tcctagccgc
       61 gccgcgggcc ggcgactgca gcttcccctg ctgtgcctct tcctccaggg cgccactgcc
      121 gtcctctttg ctgtctttgt ccgctacaac cacaaaaccg acgctgccct ctggcaccgg
      181 agcaaccaca gtaacgcgga caatgaattt tactttcgct acccaaaaga gtctcactct
      241 gttgcccagg ctggagtgca acggcgcaat ctcggctcac tgcaaccttc acctcccagg
      301 cttccaggac gtgcatgcca tggtcttcgt gggctttggc ttcctcatgg tcttcctgca
      361 gcgttacggc ttcagcagcg tgggcttcac cttcctcctg gccgcctttg ccctgcagtg
      421 gtccacactg gtccagggct ttctccactc cttccacggt ggccacatcc atgttggcgt
      481 ggagagcatg atcaatgctg acttttgtgc gggggccgtg ctcatctcct ttggtgccgt
      541 cctgggcaag accgggccta cccagctgct gctcatggcc ctgctggagg tggtgctgtt
      601 tggcatcaat gagtttgtgc tccttcatct cctgggggtg agagatgccg gaggctccat
      661 gactatccac acctttggtg cctacttcgg gctcgtcctt tcgcgggttc tgtacaggcc
      721 ccagctggag aagagcaagc accgccaggg ctccgtctac cattcagacc tcttcgccat
      781 gattgggacc atcttcctgt ggatcttctg gcctagcttc aatgctgcac tcacagcgct
      841 gggggctggg cagcatcgga cggccctcaa cacatactac tccctggctg ccagcaccct
      901 tggcaccttt gccttgtcag cccttgtagg ggaagatggg aggcttgaca tggtccacat
      961 ccaaaatgca gcgctggctg gaggggttgt ggtggggacc tcaagtgaaa tgatgctgac
     1021 accctttggg gctctggcag ctggcttctt ggctgggact gtctccacgc tggggtacaa
     1081 gttcttcacg cccatccttg aatcaaaatt caaagtccaa gacacatgtg gagtccacaa
     1141 cctccatggg atgccggggg tcctgggggc cctcctgggg gtccttgtgg ctggacttgc
     1201 cacccatgaa gcttacggag atggcctgga gagtgtgttt ccactcatag ccgagggcca
     1261 gcgcagtgcc acgtcacagg ccatgcacca gctcttcggg ctgtttgtca cactgatgtt
     1321 tgcctctgtg ggcgggggcc ttggagggct cctgctgaag ctaccctttc tggactcccc
     1381 cccagactcc cagcactacg aggaccaagt tcactggcag gtgcctggcg agcatgagga
     1441 taaagcccag agacctctga gggtggagga ggcagacact caggcctaac ccactgccag
     1501 cccctgagag gacacgctcc ttttcgaaga tgctgactgg ctgctactag gaagttcttt
     1561 ttgagctccc attcctccag ctgcaagaag ggagccatga gccagaagga ggcccctttc
     1621 cacaggcagc gtctccacag ggagaggggc aacaggaggc tgggaaatgg tggggagtgg
     1681 ggccgtaact gggtacaata gggggaacct caccagatgc ccaacccgac tgccctacca
     1741 gcctgcacat gggtagaaga ggccaaattg aggcacccaa gtgatccact ggccccacgt
     1801 cacacagtta cagtgaagcc caagccaggc ctggttgagg gtgataaacg ccactgtctc
     1861 taa
//
