LOCUS       NM_001204417            5339 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens regulator of G protein signaling 6 (RGS6), transcript
            variant 3, mRNA.
ACCESSION   NM_001204417
VERSION     NM_001204417.3
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Sengar,A.S., Kumar,M., Rai,C., Chakraborti,S., Kumar,D., Kumar,P.,
            Mukherjee,S., Mondal,K., Stewart,A. and Maity,B.
  TITLE     RGS6 drives cardiomyocyte death following nucleolar stress by
            suppressing Nucleolin/miRNA-21
  JOURNAL   J Transl Med 22 (1), 204 (2024)
   PUBMED   38409136
  REMARK    GeneRIF: RGS6 drives cardiomyocyte death following nucleolar stress
            by suppressing Nucleolin/miRNA-21.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  AUTHORS   Wang,Z., Chen,J., Wang,S., Sun,Z., Lei,Z., Zhang,H.T. and Huang,J.
  TITLE     RGS6 suppresses TGF-beta-induced epithelial-mesenchymal transition
            in non-small cell lung cancers via a novel mechanism dependent on
            its interaction with SMAD4
  JOURNAL   Cell Death Dis 13 (7), 656 (2022)
   PUBMED   35902557
  REMARK    GeneRIF: RGS6 suppresses TGF-beta-induced epithelial-mesenchymal
            transition in non-small cell lung cancers via a novel mechanism
            dependent on its interaction with SMAD4.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5339)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 5339)
  AUTHORS   Luo,Z., Ahlers-Dannen,K.E., Spicer,M.M., Yang,J., Alberico,S.,
            Stevens,H.E., Narayanan,N.S. and Fisher,R.A.
  TITLE     Age-dependent nigral dopaminergic neurodegeneration and
            alpha-synuclein accumulation in RGS6-deficient mice
  JOURNAL   JCI Insight 5 (13), 126769 (2019)
   PUBMED   31120439
  REMARK    GeneRIF: Age-dependent nigral dopaminergic neurodegeneration and
            alpha-synuclein accumulation in RGS6-deficient mice.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5339)
  AUTHORS   Guo,Y.X., Xu,Y.H., Zheng,G.H. and Jin,X.Q.
  TITLE     The novel gene HA117 promotes in vitro and in vivo drug resistance
            in mouse colon tumor cells
  JOURNAL   Cancer Gene Ther 24 (7), 304-308 (2017)
   PUBMED   28731026
  REMARK    GeneRIF: the results suggest that HA117 regulates the development
            of drug resistance in CT26 cells in vitro and in vivo.
REFERENCE   6  (bases 1 to 5339)
  AUTHORS   Chatterjee,T.K., Liu,Z. and Fisher,R.A.
  TITLE     Human RGS6 gene structure, complex alternative splicing, and role
            of N terminus and G protein gamma-subunit-like (GGL) domain in
            subcellular localization of RGS6 splice variants
  JOURNAL   J Biol Chem 278 (32), 30261-30271 (2003)
   PUBMED   12761221
  REMARK    GeneRIF: RGS6 gene structure, complex alternative splicing, and
            role of N terminus and G protein gamma-subunit-like (GGL) domain in
            subcellular localization of RGS6 splice variants
REFERENCE   7  (bases 1 to 5339)
  AUTHORS   Posner,B.A., Gilman,A.G. and Harris,B.A.
  TITLE     Regulators of G protein signaling 6 and 7. Purification of
            complexes with gbeta5 and assessment of their effects on g
            protein-mediated signaling pathways
  JOURNAL   J Biol Chem 274 (43), 31087-31093 (1999)
   PUBMED   10521509
REFERENCE   8  (bases 1 to 5339)
  AUTHORS   Snow,B.E., Betts,L., Mangion,J., Sondek,J. and Siderovski,D.P.
  TITLE     Fidelity of G protein beta-subunit association by the G protein
            gamma-subunit-like domains of RGS6, RGS7, and RGS11
  JOURNAL   Proc Natl Acad Sci U S A 96 (11), 6489-6494 (1999)
   PUBMED   10339615
REFERENCE   9  (bases 1 to 5339)
  AUTHORS   Seki,N., Hattori,A., Hayashi,A., Kozuma,S., Hori,T. and Saito,T.
  TITLE     The human regulator of G-protein signaling protein 6 gene (RGS6)
            maps between markers WI-5202 and D14S277 on chromosome 14q24.3
  JOURNAL   J Hum Genet 44 (2), 138-140 (1999)
   PUBMED   10083744
REFERENCE   10 (bases 1 to 5339)
  AUTHORS   Sherrington,R., Rogaev,E.I., Liang,Y., Rogaeva,E.A., Levesque,G.,
            Ikeda,M., Chi,H., Lin,C., Li,G., Holman,K., Tsuda,T., Mar,L.,
            Foncin,J.F., Bruni,A.C., Montesi,M.P., Sorbi,S., Rainero,I.,
            Pinessi,L., Nee,L., Chumakov,I., Pollen,D., Brookes,A., Sanseau,P.,
            Polinsky,R.J., Wasco,W., Da Silva,H.A., Haines,J.L.,
            Perkicak-Vance,M.A., Tanzi,R.E., Roses,A.D., Fraser,P.E.,
            Rommens,J.M. and St George-Hyslop,P.H.
  TITLE     Cloning of a gene bearing missense mutations in early-onset
            familial Alzheimer's disease
  JOURNAL   Nature 375 (6534), 754-760 (1995)
   PUBMED   7596406
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC005993.2, AC005477.1,
            AC005157.1, AC005533.2 and AC004828.2.
            
            On May 4, 2019 this sequence version replaced NM_001204417.2.
            
            Summary: This gene encodes a member of the RGS (regulator of G
            protein signaling) family of proteins, which are defined by the
            presence of a RGS domain that confers the GTPase-activating
            activity of these proteins toward certain G alpha subunits. This
            protein also belongs to a subfamily of RGS proteins characterized
            by the presence of DEP and GGL domains, the latter a G beta
            5-interacting domain. The RGS proteins negatively regulate G
            protein signaling, and may modulate neuronal, cardiovascular,
            lymphocytic activities, and cancer risk. Many alternatively spliced
            transcript variants encoding different isoforms with long or short
            N-terminal domains, complete or incomplete GGL domains, and
            distinct C-terminal domains, have been described for this gene,
            however, the full-length nature of some of these variants is not
            known.[provided by RefSeq, Mar 2011].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA2467143,
                              SAMEA2467144 [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-81                AC005993.2         13141-13221         c
            82-185              AC005477.1         87628-87731         c
            186-285             AC005157.1         72588-72687         c
            286-336             AC005533.2         52351-52401         c
            337-443             AC005533.2         48552-48658         c
            444-495             AC005533.2         47246-47297         c
            496-560             AC005533.2         41107-41171         c
            561-637             AC005533.2         36846-36922         c
            638-719             AC005533.2         33976-34057         c
            720-794             AC005533.2         32230-32304         c
            795-893             AC005533.2         30089-30187         c
            894-955             AC005533.2         28600-28661         c
            956-1066            AC005533.2         11667-11777         c
            1067-1192           AC004828.2         179699-179824       c
            1193-1379           AC004828.2         171441-171627       c
            1380-1469           AC004828.2         153703-153792       c
            1470-1523           AC004828.2         149884-149937       c
            1524-5339           AC004828.2         123448-127263       c
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q24.2"
     gene            1..5339
                     /gene="RGS6"
                     /gene_synonym="GAP; HA117; S914"
                     /note="regulator of G protein signaling 6"
                     /db_xref="GeneID:9628"
                     /db_xref="HGNC:HGNC:10002"
                     /db_xref="MIM:603894"
     exon            1..81
                     /gene="RGS6"
                     /gene_synonym="GAP; HA117; S914"
                     /inference="alignment:Splign:2.1.0"
     exon            82..185
                     /gene="RGS6"
                     /gene_synonym="GAP; HA117; S914"
                     /inference="alignment:Splign:2.1.0"
     CDS             102..1559
                     /gene="RGS6"
                     /gene_synonym="GAP; HA117; S914"
                     /note="isoform 3 is encoded by transcript variant 3;
                     regulator of G-protein signalling 6"
                     /codon_start=1
                     /product="regulator of G-protein signaling 6 isoform 3"
                     /protein_id="NP_001191346.1"
                     /db_xref="GeneID:9628"
                     /db_xref="HGNC:HGNC:10002"
                     /db_xref="MIM:603894"
                     /translation="MAQGSGDQRAVGVADPEESSPNMIVYCKIEDIITKMQDDKTGGV
                     PIRTVKSFLSKIPSVVTGTDIVQWLMKNLSIEDPVEAIHLGSLIAAQGYIFPISDHVL
                     TMKDDGTFYRFQAPYFWPSNCWEPENTDYAIYLCKRTMQNKARLELADYEAENLARLQ
                     RAFARKWEFIFMQAEAQVKIDRKKDKTERKILDSQERAFWDVHRPVPGCVNTTEMDIR
                     KCRRLKNPQKVKKSVYGVTEESQAQSPVHVLSQPIRKTTKEDIRKQITFLNAQIDRHC
                     LKMSKVAESLIAYTEQYVEYDPLITPAEPSNPWISDDVALWDIEMSKEPSQQRVKRWG
                     FSFDEILKDQVGRDQFLRFLESEFSSENLRFWLAVQDLKKQPLQDVAKRVEEIWQEFL
                     APGAPSAINLDSHSYEITSQNVKDGGRYTFEDAQEHIYKLMKSDSYARFLRSNAYQDL
                     LLAKKKPESEQGRRTSLEKFTRSVLYSNTPLAKRP"
     exon            186..285
                     /gene="RGS6"
                     /gene_synonym="GAP; HA117; S914"
                     /inference="alignment:Splign:2.1.0"
     exon            286..336
                     /gene="RGS6"
                     /gene_synonym="GAP; HA117; S914"
                     /inference="alignment:Splign:2.1.0"
     exon            337..443
                     /gene="RGS6"
                     /gene_synonym="GAP; HA117; S914"
                     /inference="alignment:Splign:2.1.0"
     exon            444..495
                     /gene="RGS6"
                     /gene_synonym="GAP; HA117; S914"
                     /inference="alignment:Splign:2.1.0"
     exon            496..560
                     /gene="RGS6"
                     /gene_synonym="GAP; HA117; S914"
                     /inference="alignment:Splign:2.1.0"
     exon            561..637
                     /gene="RGS6"
                     /gene_synonym="GAP; HA117; S914"
                     /inference="alignment:Splign:2.1.0"
     exon            638..719
                     /gene="RGS6"
                     /gene_synonym="GAP; HA117; S914"
                     /inference="alignment:Splign:2.1.0"
     exon            720..794
                     /gene="RGS6"
                     /gene_synonym="GAP; HA117; S914"
                     /inference="alignment:Splign:2.1.0"
     exon            795..893
                     /gene="RGS6"
                     /gene_synonym="GAP; HA117; S914"
                     /inference="alignment:Splign:2.1.0"
     exon            894..955
                     /gene="RGS6"
                     /gene_synonym="GAP; HA117; S914"
                     /inference="alignment:Splign:2.1.0"
     exon            956..1066
                     /gene="RGS6"
                     /gene_synonym="GAP; HA117; S914"
                     /inference="alignment:Splign:2.1.0"
     exon            1067..1192
                     /gene="RGS6"
                     /gene_synonym="GAP; HA117; S914"
                     /inference="alignment:Splign:2.1.0"
     exon            1193..1379
                     /gene="RGS6"
                     /gene_synonym="GAP; HA117; S914"
                     /inference="alignment:Splign:2.1.0"
     exon            1380..1469
                     /gene="RGS6"
                     /gene_synonym="GAP; HA117; S914"
                     /inference="alignment:Splign:2.1.0"
     exon            1470..1523
                     /gene="RGS6"
                     /gene_synonym="GAP; HA117; S914"
                     /inference="alignment:Splign:2.1.0"
     exon            1524..5339
                     /gene="RGS6"
                     /gene_synonym="GAP; HA117; S914"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2738..2743
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RGS6"
                     /gene_synonym="GAP; HA117; S914"
                     /note="hexamer: AACAAA"
     polyA_site      2757
                     /gene="RGS6"
                     /gene_synonym="GAP; HA117; S914"
                     /note="major polyA site"
     regulatory      5311..5316
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RGS6"
                     /gene_synonym="GAP; HA117; S914"
                     /note="hexamer: AATAAA"
     polyA_site      5339
                     /gene="RGS6"
                     /gene_synonym="GAP; HA117; S914"
                     /note="major polyA site"
ORIGIN      
        1 gtgaagcgca caccgacggg gacgagagcg cagaggatgg cgcttttaga tacttccagt
       61 cttccgatgt tgtgatcgca gtgtgagtga agacactcag gatggctcaa ggatccgggg
      121 atcaaagagc agtgggggtt gctgacccag aggagagttc tccaaacatg atcgtttact
      181 gcaaaattga agacatcatt acaaagatgc aagatgacaa gacagggggt gtgcccatca
      241 gaacagtcaa gagctttctc tccaaaatcc ccagtgtcgt cacaggtact gacattgtgc
      301 agtggcttat gaagaacctt tccattgagg acccagttga agcaatacac ttggggagcc
      361 ttatcgctgc ccagggctac atctttccaa tctcagacca tgttctcacc atgaaggatg
      421 atggcacctt ttatcgtttc caggctccgt acttctggcc ttcgaactgc tgggaacctg
      481 aaaacactga ctatgccatc tatctctgta agaggacaat gcaaaataaa gcaaggctgg
      541 aactggcaga ttatgaagca gaaaacttag caagactcca gagggccttt gcgaggaagt
      601 gggaattcat ctttatgcaa gcagaagcac aagtaaagat tgaccggaaa aaagacaaga
      661 cagaaaggaa aattttggat agtcaagaac gagccttttg ggatgtccac aggcctgtgc
      721 caggctgtgt gaacacaaca gaaatggata tccgaaaatg tcgacgtttg aagaatccac
      781 aaaaggttaa aaagtccgtg tatggcgtga ctgaagagtc ccaggcacag agcccggtgc
      841 atgtactcag ccaaccaatc aggaaaacaa caaaagagga catccggaaa cagataacat
      901 ttttgaacgc acagatcgac agacattgtt tgaaaatgtc caaagtggct gaaagtttaa
      961 ttgcctacac ggaacaatat gtggaatatg accctttgat aacaccagct gagccatcca
     1021 acccttggat cagcgatgac gttgctttgt gggacataga gatgagcaaa gagcccagcc
     1081 aacagcgagt aaaaagatgg ggcttctctt tcgatgagat attgaaggac caggtggggc
     1141 gggaccagtt tctacgattc ctggagtccg aattcagttc agaaaacctc aggttctggc
     1201 tggctgtcca agatcttaag aaacaacccc tacaggatgt ggccaagagg gtagaagaaa
     1261 tctggcaaga gtttctggct ccaggggctc caagtgcaat caacctggat tctcacagct
     1321 atgagataac cagtcaaaat gtcaaagatg gagggagata tacatttgaa gacgcccagg
     1381 agcacatcta caagctgatg aagagtgaca gctatgcccg cttcctccgg tcaaatgctt
     1441 accaggattt gctgctggcc aagaagaagc cagaaagtga gcaaggtcgt agaacttccc
     1501 tagaaaagtt cactcgcagt gtgttgtatt ccaacactcc actcgctaag aggccctgat
     1561 cccagctcat tcaggggaga acacgtcgtg gggttcctgt cacgactatc acttgagatt
     1621 atattattat cctcactccc tcgctgtctg gagacggtca caccttctgg caaattcaag
     1681 aggcatgagt ggaccgagag cacatccagc atttgcatca cctccctggc acctcccata
     1741 gttacagcca ctacatcccc accgccgcct gtcatccctc acgtctctgt ggccacccgg
     1801 gtgtcactgc cagcaccagg cactgctttc acgtaaaatg tccaaatcac atcttcagca
     1861 agaaagcaac ctcacggatt gccccgcctc aaggtctttc caccctcttt gagatcagcg
     1921 ttcggagagg tccccgccag cccggtggct gccctcaggt cccgtcacat gtctcaaggg
     1981 tccagcgttc gaggaagcac tgttgtagat gacagagctt tttgtttcac agctgcgggg
     2041 attctgctga ccatctttta gaaaattatt tcataattta ttctggcttt gcccccttgc
     2101 tccttggttt attccctcat gttttactca aaataaacat ggccatgaac tcaggggcca
     2161 ttgggattct cccctctggc agccccaggt gggagctggg agggtcccca gcccaggagg
     2221 tggctggatt gcagggctgg cagcccccac gtggtggcat cctgacaggc acagtgagac
     2281 ccttgctggg tcaggtctcc tttgtgtgtg aagctggggc agagtatgct acgaagggac
     2341 caggccaggc cacagggctc tcctcccagt ctttatctga aggcccagcc aaagcacccc
     2401 tctaaaccgg tcacctaggg gaaatgcaaa aacctgcaaa agatccacct gtattccctg
     2461 tgtgtgtgtg tcaaaatgac catttgccaa cctggctgtg aggtaatgta tgtaaagcac
     2521 cgagtcaggt gcctgatacg cagtaggtgc ttagcaaacg gtagctaata ctatcatcat
     2581 tgtcattgtt attgtgacac ccatgagccc acagacagag ctgtattaag taacctgttt
     2641 tgaatccaga cattcactcc cccacttgct atgactaccc tcctttgatc tatttttggt
     2701 cataggtaaa aaaaaaaatc tctcttggga gcatataaac aaaaaacata gaagcaataa
     2761 agcgatcgct ggtatttctt ttctcatgca gccaaattcg ccagctggtg accaaaggct
     2821 cagatgcttg cttatttgca gacccggcct cctgggtgct ccccagtgac ccatctttcc
     2881 accccaccac ctccaagtcc tgtctatgtc tgcttccaca cagccctgtc cccagggcac
     2941 cagccttctg tccctcacag cagtgccatc gcgggcatta ggagtcttcg ttatgttgta
     3001 tccttgtgag ttcttgacta ccatgcccaa gtttccccca ctggcaatgg ttcctctctt
     3061 ggaggtacag tggaaaccag agagatgaca gatgttgagt cctcagtgtc tcagaggcag
     3121 caattcttgc tggacacctg cacatgctca gcacagaggc tggtactttg gaggcactaa
     3181 ccacttgatg agtaaatcaa tggcataaag ccccattcca agttatgggg tcttttgaca
     3241 ggcccctttc atcctctttc tctttgtata aacctcatgt ttcagaacaa aattacagtc
     3301 taagttcatt tgcccccagc cttgcccaag gatccccggg ctgtgctgac ccctaggcat
     3361 agggcaaagg gtgttcccaa gcagcaggca cctcagtgcc ctcagttatc cagggcaagg
     3421 ggcctggctg ccccctgctc ctccccatga tgcaggggct ttctaagcca ggctgcagac
     3481 agggcagggt tggcttcttg gccattccct ctgtgccccc ttctccttct gactggtctc
     3541 accctggaga tccccagagg atctgaccag agccaggtgg actgtggccc tgagatggag
     3601 ctggctgcag gcttacaagc acctctgggg acatccagag gggagggtca gtaagcacaa
     3661 cggagctagg gagacttcag atggactcaa acccagagca ataatactct cacaccaccc
     3721 cacagctaag gcactcatgt tccccagtag atcaagccag aagctttgca agaagggagt
     3781 ggaacgaggc tgcctatcca aggtccatct accacgcaga acttggtcat gcaagtacat
     3841 ttggaaaatc tggtcttggc ccgtccccca ttggcctggt ctacacagcc aaaaattcag
     3901 actagattag atgtttgcag gcaattctgt agcaaagcaa attcccctac cccgtccctt
     3961 ccccctagaa gctgccttag cctccctgac tgggtgtggt ctctgggcct aggactcctc
     4021 cctatgagga aaagtgctct ccagccacat cttggcagcc ccacaaccac agcatcaggg
     4081 cagctgcagc aagtgaccac ccacccgtcg ggttagacac acaaggcacc aggcactggc
     4141 atctgactaa aataaccttt ccctacatgg gaagaggaaa gcttgtaggc tggcacatat
     4201 aaacgtccag ccgcagccag atggcctgaa ctaagcagca ggaactctct gggctgggga
     4261 ggagcctcct gcccttctag aaagtgatgc gtcagctttg ttctcctggg aaggtgttgc
     4321 tataccccag gaacaaagca gggcccaact gccagtgcag tcagctgggc ctgcgctcca
     4381 ggctcaccac cgctaagtgg tcaggtaccg ggccagcatc ctgggaggga cctcttgcga
     4441 ctctgagttg cagcttggac tagctgaaca catggggaca tgctccaggg gcttcttccc
     4501 tggcaggatt cctgggattt caaactacct caccaacagg acgctgaacc aggagcctgc
     4561 cacccaactg gcccccttgg tgattgtgga aggtgatgtt tttcctaaaa tgcatccctc
     4621 ctgttctgct ccatatacat gtgcttcaaa aaccaccacc aggaaagcct tcccagaaga
     4681 gggaaaggat taagggctct cttcctttgg gccctgcaga atgtattgga gggaggtgtt
     4741 gctgcaaggt ccaggagctg ggaaactctg ggctcggggg gagataatgc accaccagcc
     4801 tcaaaaagga gctgcccaag ttgaatatgt agcaaagacc atgggataag ttgaatagtg
     4861 gccagacgaa tcactaataa ctcaccatgg gaccctctga agagaggcct gtgctcgtgt
     4921 aaccatagca acagcagatg ccagcaacct actcattagt accacccagt gataaggcat
     4981 cccctgggct agacccaggg gccataaagt agcaaagtag caaatgtagc aataaagggc
     5041 ccgggtctat acggggacag ggtccaaatg accaggactt ggccctctcc ccagccccga
     5101 tgagggctca gagccagctg ctgtcagctg tctggggagg gaaggccctg ctccatacca
     5161 ggggtccctg ttctatactc taagcccact caccgcatga tcacggactc atcggaatgg
     5221 cctcctctta gtgcatgtgg ttctctttag tttatttttc tggaatttgg ggattggacc
     5281 ccagaaccaa acatgctggt acagtctaaa aataaatgac ttgaacccac ttcaatcca
//
