LOCUS       NM_005613               2984 bp    mRNA    linear   PRI 13-MAY-2024
DEFINITION  Homo sapiens regulator of G protein signaling 4 (RGS4), transcript
            variant 2, mRNA.
ACCESSION   NM_005613
VERSION     NM_005613.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Joshi,I.V., Chan,E.C., Lack,J.B., Liu,C. and Druey,K.M.
  TITLE     RGS4 controls airway hyperresponsiveness through GAP-independent
            mechanisms
  JOURNAL   J Biol Chem 300 (4), 107127 (2024)
   PUBMED   38432633
  REMARK    GeneRIF: RGS4 controls airway hyperresponsiveness through
            GAP-independent mechanisms.
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Pisano,C.A., Mercatelli,D., Mazzocchi,M., Brugnoli,A., Morella,I.,
            Fasano,S., Zaveri,N.T., Brambilla,R., O'Keeffe,G.W., Neubig,R.R.
            and Morari,M.
  TITLE     Regulator of G-Protein Signalling 4 (RGS4) negatively modulates
            nociceptin/orphanin FQ opioid receptor signalling: Implication for
            l-Dopa-induced dyskinesia
  JOURNAL   Br J Pharmacol 180 (7), 927-942 (2023)
   PUBMED   34767639
  REMARK    GeneRIF: Regulator of G-Protein Signalling 4 (RGS4) negatively
            modulates nociceptin/orphanin FQ opioid receptor signalling:
            Implication for l-Dopa-induced dyskinesia.
REFERENCE   3  (bases 1 to 2984)
  AUTHORS   Borges,J.I., Suster,M.S. and Lymperopoulos,A.
  TITLE     Cardiac RGS Proteins in Human Heart Failure and Atrial
            Fibrillation: Focus on RGS4
  JOURNAL   Int J Mol Sci 24 (7), 6136 (2023)
   PUBMED   37047106
  REMARK    GeneRIF: Cardiac RGS Proteins in Human Heart Failure and Atrial
            Fibrillation: Focus on RGS4.
            Review article
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2984)
  AUTHORS   Carbone,A.M., Borges,J.I., Suster,M.S., Sizova,A., Cora,N.,
            Desimine,V.L. and Lymperopoulos,A.
  TITLE     Regulator of G-Protein Signaling-4 Attenuates Cardiac Adverse
            Remodeling and Neuronal Norepinephrine Release-Promoting Free Fatty
            Acid Receptor FFAR3 Signaling
  JOURNAL   Int J Mol Sci 23 (10), 5803 (2022)
   PUBMED   35628613
  REMARK    GeneRIF: Regulator of G-Protein Signaling-4 Attenuates Cardiac
            Adverse Remodeling and Neuronal Norepinephrine Release-Promoting
            Free Fatty Acid Receptor FFAR3 Signaling.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2984)
  AUTHORS   Kim,Y. and Ghil,S.
  TITLE     Regulators of G-protein signaling, RGS2 and RGS4, inhibit
            protease-activated receptor 4-mediated signaling by forming a
            complex with the receptor and Galpha in live cells
  JOURNAL   Cell Commun Signal 18 (1), 86 (2020)
   PUBMED   32517689
  REMARK    GeneRIF: Regulators of G-protein signaling, RGS2 and RGS4, inhibit
            protease-activated receptor 4-mediated signaling by forming a
            complex with the receptor and Galpha in live cells.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2984)
  AUTHORS   Druey,K.M., Sullivan,B.M., Brown,D., Fischer,E.R., Watson,N.,
            Blumer,K.J., Gerfen,C.R., Scheschonka,A. and Kehrl,J.H.
  TITLE     Expression of GTPase-deficient Gialpha2 results in translocation of
            cytoplasmic RGS4 to the plasma membrane
  JOURNAL   J Biol Chem 273 (29), 18405-18410 (1998)
   PUBMED   9660808
REFERENCE   7  (bases 1 to 2984)
  AUTHORS   Srinivasa,S.P., Bernstein,L.S., Blumer,K.J. and Linder,M.E.
  TITLE     Plasma membrane localization is required for RGS4 function in
            Saccharomyces cerevisiae
  JOURNAL   Proc Natl Acad Sci U S A 95 (10), 5584-5589 (1998)
   PUBMED   9576926
REFERENCE   8  (bases 1 to 2984)
  AUTHORS   Heximer,S.P., Watson,N., Linder,M.E., Blumer,K.J. and Hepler,J.R.
  TITLE     RGS2/G0S8 is a selective inhibitor of Gqalpha function
  JOURNAL   Proc Natl Acad Sci U S A 94 (26), 14389-14393 (1997)
   PUBMED   9405622
REFERENCE   9  (bases 1 to 2984)
  AUTHORS   Berman,D.M., Wilkie,T.M. and Gilman,A.G.
  TITLE     GAIP and RGS4 are GTPase-activating proteins for the Gi subfamily
            of G protein alpha subunits
  JOURNAL   Cell 86 (3), 445-452 (1996)
   PUBMED   8756726
REFERENCE   10 (bases 1 to 2984)
  AUTHORS   Druey,K.M., Blumer,K.J., Kang,V.H. and Kehrl,J.H.
  TITLE     Inhibition of G-protein-mediated MAP kinase activation by a new
            mammalian gene family
  JOURNAL   Nature 379 (6567), 742-746 (1996)
   PUBMED   8602223
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DQ346663.1 and AL583850.5.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 21, 2020 this sequence version replaced NM_005613.5.
            
            Summary: Regulator of G protein signaling (RGS) family members are
            regulatory molecules that act as GTPase activating proteins (GAPs)
            for G alpha subunits of heterotrimeric G proteins. RGS proteins are
            able to deactivate G protein subunits of the Gi alpha, Go alpha and
            Gq alpha subtypes. They drive G proteins into their inactive
            GDP-bound forms. Regulator of G protein signaling 4 belongs to this
            family. All RGS proteins share a conserved 120-amino acid sequence
            termed the RGS domain. Regulator of G protein signaling 4 protein
            is 37% identical to RGS1 and 97% identical to rat Rgs4. This
            protein negatively regulate signaling upstream or at the level of
            the heterotrimeric G protein and is localized in the cytoplasm.
            Alternatively spliced transcript variants have been found for this
            gene. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (2) has an alternate 5' sequence,
            which results in a downstream AUG start codon, as compared to
            variant 1. The resulting isoform (2) has a shorter N-terminus,
            compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DQ346663.1, DQ346662.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000367909.11/ ENSP00000356885.6
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1308              DQ346663.1         216-1523
            1309-2984           AL583850.5         87877-89552
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q23.3"
     gene            1..2984
                     /gene="RGS4"
                     /gene_synonym="RGP4; SCZD9"
                     /note="regulator of G protein signaling 4"
                     /db_xref="GeneID:5999"
                     /db_xref="HGNC:HGNC:10000"
                     /db_xref="MIM:602516"
     exon            1..168
                     /gene="RGS4"
                     /gene_synonym="RGP4; SCZD9"
                     /inference="alignment:Splign:2.1.0"
     CDS             125..742
                     /gene="RGS4"
                     /gene_synonym="RGP4; SCZD9"
                     /note="isoform 2 is encoded by transcript variant 2;
                     schizophrenia disorder 9"
                     /codon_start=1
                     /product="regulator of G-protein signaling 4 isoform 2"
                     /protein_id="NP_005604.1"
                     /db_xref="CCDS:CCDS1243.1"
                     /db_xref="GeneID:5999"
                     /db_xref="HGNC:HGNC:10000"
                     /db_xref="MIM:602516"
                     /translation="MCKGLAGLPASCLRSAKDMKHRLGFLLQKSDSCEHNSSHNKKDK
                     VVICQRVSQEEVKKWAESLENLISHECGLAAFKAFLKSEYSEENIDFWISCEEYKKIK
                     SPSKLSPKAKKIYNEFISVQATKEVNLDSCTREETSRNMLEPTITCFDEAQKKIFNLM
                     EKDSYRRFLKSRFYLDLVNPSSCGAEKQKGAKSSADCASLVPQCA"
     exon            169..273
                     /gene="RGS4"
                     /gene_synonym="RGP4; SCZD9"
                     /inference="alignment:Splign:2.1.0"
     exon            274..335
                     /gene="RGS4"
                     /gene_synonym="RGP4; SCZD9"
                     /inference="alignment:Splign:2.1.0"
     exon            336..502
                     /gene="RGS4"
                     /gene_synonym="RGP4; SCZD9"
                     /inference="alignment:Splign:2.1.0"
     exon            503..2984
                     /gene="RGS4"
                     /gene_synonym="RGP4; SCZD9"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2754..2759
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RGS4"
                     /gene_synonym="RGP4; SCZD9"
                     /note="hexamer: AGTAAA"
     polyA_site      2776
                     /gene="RGS4"
                     /gene_synonym="RGP4; SCZD9"
     regulatory      2962..2967
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RGS4"
                     /gene_synonym="RGP4; SCZD9"
                     /note="hexamer: AATAAA"
     polyA_site      2984
                     /gene="RGS4"
                     /gene_synonym="RGP4; SCZD9"
                     /note="major polyA site"
ORIGIN      
        1 aagacgctca gaggattctg acaatatctt taccggagaa gaggcaaagt acgctcaaag
       61 ccgaagccac agctcctcct gccgcatttc tttcctgctt gcgaattcca agctgttaaa
      121 taagatgtgc aaagggcttg caggtctgcc ggcttcttgc ttgaggagtg caaaagatat
      181 gaaacatcgg ctaggtttcc tgctgcaaaa atctgattcc tgtgaacaca attcttccca
      241 caacaagaag gacaaagtgg ttatttgcca gagagtgagc caagaggaag tcaagaaatg
      301 ggctgaatca ctggaaaacc tgattagtca tgaatgtggg ctggcagctt tcaaagcttt
      361 cttgaagtct gaatatagtg aggagaatat tgacttctgg atcagctgtg aagagtacaa
      421 gaaaatcaaa tcaccatcta aactaagtcc caaggccaaa aagatctata atgaattcat
      481 ctcagtccag gcaaccaaag aggtgaacct ggattcttgc accagggaag agacaagccg
      541 gaacatgcta gagcctacaa taacctgctt tgatgaggcc cagaagaaga ttttcaacct
      601 gatggagaag gattcctacc gccgcttcct caagtctcga ttctatcttg atttggtcaa
      661 cccgtccagc tgtggggcag aaaagcagaa aggagccaag agttcagcag actgtgcttc
      721 cctggtccct cagtgtgcct aattctcacc tgaaggcaga gggatgaaat gccaagactc
      781 tatgctctgg aaaacctgag gccaaatatt gatctgtatt aagctccagt gctttatcca
      841 cattgtagcc taatattcat gctgcctgcc atgtgtgagt cacttctacg cataaactag
      901 atatagcttt tggtgtttga gtgttcatca gggtgggacc ccattccagt ccaattttcc
      961 taagtttctt tgagggttcc atgggagcaa atatctaaat aatggcctgg taggtctgga
     1021 ttttcaaaga ttgttggcag tttcctcctc ccaacagttt tacctcggga tggttggtta
     1081 gtgcatgtca catgacatcc acatgcacat gtattctgtt ggccagcacg ttctccagac
     1141 tctagatgtt tagatgaggt tgagctatga tatgtgcttg tgtgtatgtc tatgtgtata
     1201 tattatatat acattagaca cacatataca ttatttctgt atatagatgt ctgtgtatac
     1261 atatgtatgt gtgagtgtat gtatacacac acacacacac acacacacac acttttgcaa
     1321 gagtgatggg aaagacccta ggtgctcata actagagtat gtgtatgtac ttacatgggt
     1381 gttttgatct ctgttctttc atactacatt tgaacagggc aaaatgaact aactgccatg
     1441 taggctaaga aagaaatgct aacctgtgga aagttggttt tgtaaaattc catggatctt
     1501 gctggagaag catccaagga acttcatgct tgatttgacc actgacagcc tccaccttga
     1561 gcactattct aaggagcaaa taccttagct cccttgagct ggttttctct gatggcactt
     1621 ttgagctcct aagctgccag ccttcccttc ttttcctggg tgctcagggc atgcttatta
     1681 gcagctgggt tggtatggag ttggcagaca ggatgttcaa cttaatgaag aaatacagct
     1741 aaggccttgc cagcaacacc tgccgtaagt tactggctga gtgagggcat agaagttaaa
     1801 ggttactgtt tttatcctct atcctttttt cctttcctga tcaaggtgct cttctcattt
     1861 tttcctgaga accttagcca tcagatgagg ctccttagtt tattgtggtt ggttgttttt
     1921 tctttataat ggctctgggc tatatgccta tatttataaa ccagcagcag gggaaagatt
     1981 atattttata agagggaaca aattttcaca atttgaaaag cccacataag ttttctcttt
     2041 taaggtagaa tcttgttaat ttcattccaa acatcggggc taacagagac tggaggcatt
     2101 tctttttagg ctctgagact aaatgagagg aaaagaaaag aaaaaaaaaa tgattgtcta
     2161 accaattgtg agaattactg tttgaaactt ttcaaggcac attgaaatac ttgaaaactt
     2221 ctcatttatg ttatttatga tgttattttg tacgtgttat tattattata ttgttttata
     2281 aatggaggta caggatatca cctgaattat taatgaatgc ccaggaagta attttcttct
     2341 cattcttcta aaactactgc ctttcaaagt gcacacacac gcgtccacat acactgcatt
     2401 cgttgctcca gtataaatta catgcatgag cacctttctg gcttttaagc caatataatg
     2461 ggctgcaaaa tgaagacacc agagtgtatg catacaaatc tcactgtatt aaagatgcag
     2521 gttttctaat tgtacccttc ttgtctctct ggcaatcttg cccttaatat ccctggagtt
     2581 cctcatcagt gtcattttct gttatacaca gttccacaat tttgtctcta gttgacttca
     2641 aatgtgtaac tttattggtc ttgccctatt ataattgtca tgactttcag attgtatctg
     2701 aactcacaga ctgctgtctt actaataggt ctggaaggtc acgctgaatg agaagtaaat
     2761 tattttatgt aatacatttt tgagtgtgtt tttcagttgt atttccctgt tatttcatca
     2821 ctatttccaa tggtgagctt gcctgctcat gctccctgga cagaatactc cttccttttg
     2881 catgcctgtt tctatcatgt gcttgatagg cctcaaagct aatgcttcca gtgaaacaca
     2941 cgcatcttaa taataagggt aaataaacgc tccatatgaa acta
//
