LOCUS       NM_003702               1715 bp    mRNA    linear   PRI 22-SEP-2024
DEFINITION  Homo sapiens regulator of G protein signaling 20 (RGS20),
            transcript variant 2, mRNA.
ACCESSION   NM_003702
VERSION     NM_003702.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 1715)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1715)
  AUTHORS   Li,G., Wang,M., Ren,L., Li,H., Liu,Q., Ouyang,Y., He,L. and Li,F.
  TITLE     Regulator of G protein signaling 20 promotes proliferation and
            migration in bladder cancer via NF-kappaB signaling
  JOURNAL   Biomed Pharmacother 117, 109112 (2019)
   PUBMED   31212130
  REMARK    GeneRIF: RGS20 acted as an oncogene in bladder cancer.
REFERENCE   5  (bases 1 to 1715)
  AUTHORS   Chen,S., Fragoza,R., Klei,L., Liu,Y., Wang,J., Roeder,K., Devlin,B.
            and Yu,H.
  TITLE     An interactome perturbation framework prioritizes damaging missense
            mutations for developmental disorders
  JOURNAL   Nat Genet 50 (7), 1032-1040 (2018)
   PUBMED   29892012
REFERENCE   6  (bases 1 to 1715)
  AUTHORS   Sierra,D.A., Gilbert,D.J., Householder,D., Grishin,N.V., Yu,K.,
            Ukidwe,P., Barker,S.A., He,W., Wensel,T.G., Otero,G., Brown,G.,
            Copeland,N.G., Jenkins,N.A. and Wilkie,T.M.
  TITLE     Evolution of the regulators of G-protein signaling multigene family
            in mouse and human
  JOURNAL   Genomics 79 (2), 177-185 (2002)
   PUBMED   11829488
REFERENCE   7  (bases 1 to 1715)
  AUTHORS   Barker,S.A., Wang,J., Sierra,D.A. and Ross,E.M.
  TITLE     RGSZ1 and Ret RGS: two of several splice variants from the gene
            RGS20
  JOURNAL   Genomics 78 (3), 223-229 (2001)
   PUBMED   11735229
REFERENCE   8  (bases 1 to 1715)
  AUTHORS   Chatterjee,T.K. and Fisher,R.A.
  TITLE     Cytoplasmic, nuclear, and golgi localization of RGS proteins.
            Evidence for N-terminal and RGS domain sequences as intracellular
            targeting motifs
  JOURNAL   J Biol Chem 275 (31), 24013-24021 (2000)
   PUBMED   10791963
REFERENCE   9  (bases 1 to 1715)
  AUTHORS   Wang,J., Ducret,A., Tu,Y., Kozasa,T., Aebersold,R. and Ross,E.M.
  TITLE     RGSZ1, a Gz-selective RGS protein in brain. Structure, membrane
            association, regulation by Galphaz phosphorylation, and
            relationship to a Gz gtpase-activating protein subfamily
  JOURNAL   J Biol Chem 273 (40), 26014-26025 (1998)
   PUBMED   9748280
REFERENCE   10 (bases 1 to 1715)
  AUTHORS   Glick,J.L., Meigs,T.E., Miron,A. and Casey,P.J.
  TITLE     RGSZ1, a Gz-selective regulator of G protein signaling whose action
            is sensitive to the phosphorylation state of Gzalpha
  JOURNAL   J Biol Chem 273 (40), 26008-26013 (1998)
   PUBMED   9748279
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC113194.8 and AC100821.2.
            
            On Aug 25, 2023 this sequence version replaced NM_003702.4.
            
            Summary: The protein encoded by this gene belongs to the family of
            regulator of G protein signaling (RGS) proteins, which are
            regulatory and structural components of G protein-coupled receptor
            complexes. RGS proteins inhibit signal transduction by increasing
            the GTPase activity of G protein alpha subunits, thereby driving
            them into their inactive GDP-bound forms. This protein selectively
            binds to G(z)-alpha and G(alpha)-i2 subunits, and regulates their
            signaling activities. Alternatively spliced transcript variants
            encoding different isoforms have been found for this gene.
            [provided by RefSeq, Sep 2011].
            
            Transcript Variant: This variant (2) differs in the 5' UTR and 5'
            coding region compared to variant 1. The resulting isoform (b) has
            a shorter and distinct N-terminus compared to isoform a.
            
            Sequence Note:.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF060877.2, BC063490.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145893, SAMEA2148874
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000276500.5/ ENSP00000276500.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-213               AC113194.8         73648-73860         c
            214-362             AC113194.8         15008-15156         c
            363-446             AC113194.8         7984-8067           c
            447-681             AC113194.8         422-656             c
            682-1715            AC100821.2         145103-146136       c
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q11.23"
     gene            1..1715
                     /gene="RGS20"
                     /gene_synonym="g(z)GAP; gz-GAP; RGSZ1; ZGAP1"
                     /note="regulator of G protein signaling 20"
                     /db_xref="GeneID:8601"
                     /db_xref="HGNC:HGNC:14600"
                     /db_xref="MIM:607193"
     exon            1..213
                     /gene="RGS20"
                     /gene_synonym="g(z)GAP; gz-GAP; RGSZ1; ZGAP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             145..870
                     /gene="RGS20"
                     /gene_synonym="g(z)GAP; gz-GAP; RGSZ1; ZGAP1"
                     /note="isoform b is encoded by transcript variant 2;
                     regulator of G-protein signalling 20; regulator of
                     G-protein signaling Z1; gz-selective GTPase-activating
                     protein; regulator of Gz-selective protein signaling 1"
                     /codon_start=1
                     /product="regulator of G-protein signaling 20 isoform b"
                     /protein_id="NP_003693.2"
                     /db_xref="CCDS:CCDS6156.1"
                     /db_xref="GeneID:8601"
                     /db_xref="HGNC:HGNC:14600"
                     /db_xref="MIM:607193"
                     /translation="MRTADGGEPAGASSPAGRVDGGLQMGSERMEMRKRQMPAAQDTP
                     GAAPGQPGAGSRGSNACCFCWCCCCSCSCLTVRNQEDQRPTIASHELRADLPTWEESP
                     APTLEEVNAWAQSFDKLMVTPAGRNAFREFLRTEFSEENMLFWMACEELKKEANKNII
                     EEKARIIYEDYISILSPKEVSLDSRVREVINRNMVEPSQHIFDDAQLQIYTLMHRDSY
                     PRFMNSAVYKDLLQSLSEKSIEA"
     exon            214..362
                     /gene="RGS20"
                     /gene_synonym="g(z)GAP; gz-GAP; RGSZ1; ZGAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            363..446
                     /gene="RGS20"
                     /gene_synonym="g(z)GAP; gz-GAP; RGSZ1; ZGAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            447..681
                     /gene="RGS20"
                     /gene_synonym="g(z)GAP; gz-GAP; RGSZ1; ZGAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            682..1715
                     /gene="RGS20"
                     /gene_synonym="g(z)GAP; gz-GAP; RGSZ1; ZGAP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1690..1695
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RGS20"
                     /gene_synonym="g(z)GAP; gz-GAP; RGSZ1; ZGAP1"
                     /note="hexamer: AATAAA"
     polyA_site      1715
                     /gene="RGS20"
                     /gene_synonym="g(z)GAP; gz-GAP; RGSZ1; ZGAP1"
                     /note="major polyA site"
ORIGIN      
        1 agagcaaggg gaggaagagg ccgggagaag agggctagag caaagaccga gagccggaga
       61 aaggcgaaag gcgcggtgag caatcgccga cgtagagagg gcagccctcc gcgctgcaat
      121 attgagaagg cacccgcggg acgcatgcgc acggcggacg gaggcgagcc ggccggggct
      181 tcctccccgg ccggcagggt ggacggtggg ctccagatgg gatcagagcg gatggagatg
      241 cggaagcggc agatgcccgc cgcccaggac acaccaggcg ccgccccagg ccagcccgga
      301 gcggggagtc gcgggtccaa cgcatgctgc ttctgctggt gctgctgttg tagctgctcg
      361 tgtctcactg ttagaaacca ggaagatcag aggcccacaa tagcttccca cgaactcaga
      421 gcagatcttc caacctggga agaaagccct gctcctactc tggaagaagt caacgcctgg
      481 gctcagtcat ttgacaaatt aatggtcact ccagcaggaa ggaatgcatt ccgtgaattc
      541 ctccgaacag aattcagtga ggaaaatatg ctcttctgga tggcctgtga ggaactgaaa
      601 aaggaagcta ataaaaacat tattgaagag aaagcaagga taatctatga agactacatt
      661 tctatacttt ctcctaagga ggtgagctta gactcccggg tgagagaagt gatcaacaga
      721 aacatggtgg agccatccca acacatattc gatgatgctc aacttcagat ttacaccctg
      781 atgcacagag actcatatcc tcgattcatg aactctgctg tctataagga cttgcttcag
      841 tccttatcgg agaaatctat tgaagcatag gatttttcaa atatatttat tattaataaa
      901 ataataaaag aattcatggg ctacaactag cacagggaat ttagaggttg tagcatcttc
      961 tgctggagta atactcaggc tattctaata acagatgatt ccttcaacag actgctatat
     1021 attcaccatg taaactgcag ccacctttag tgatactttt gaaaaaaaaa aataaaggga
     1081 tatggctgtt gtagaaagat agcgtatttg catttacaat aacagtagca tgttgtcagt
     1141 ggccaaggct acacagaagg ctccctgctg cccggagcag gtacatccac cagagcaaag
     1201 ggaaccactt ttattttgca tgagtttggt aactgattac tctcccctca aagaaaagac
     1261 attcaggtgt ttctcaacga catcttctgt ccagcaagct cggtttgaat acgtcactta
     1321 ccagtgccat tgcaggaccc aaattcacag ttcataaaag atgtgaccac tacatgtaaa
     1381 aatagcattc tacttgatct tacagtatgt atgtatgtat gtatggagac atatgtgtgc
     1441 gtgtggatgt ctacatggtt aatggaaagc actgtgctct gaagtggatc agtctcaagt
     1501 gtctggtaac agcagcagtg cttagaaaat tcttttgttg aaaagagtta ctgttattat
     1561 cagaatttgc caacctaaag aatgaatttt taaattcata acttggtcat gttcacaaca
     1621 aaactgtcaa ataagcatat ttccattttt attactgaaa gaaatatggg cattttcatt
     1681 ctttaaagaa ataaagcaca agaattttat ctcaa
//
