LOCUS       NM_152869               2168 bp    mRNA    linear   PRI 19-FEB-2024
DEFINITION  Homo sapiens regucalcin (RGN), transcript variant 2, mRNA.
ACCESSION   NM_152869 XM_001126531
VERSION     NM_152869.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ghanem,N.Z. and Yamaguchi,M.
  TITLE     Regucalcin downregulation in human cancer
  JOURNAL   Life Sci 340, 122448 (2024)
   PUBMED   38246519
  REMARK    GeneRIF: Regucalcin downregulation in human cancer.
            Review article
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Munipalli,S.B. and Yenugu,S.
  TITLE     Uroplakin 1a Interacts with Regucalcin and Proteasome Subunit Beta
            1
  JOURNAL   Reprod Sci 30 (12), 3520-3528 (2023)
   PUBMED   37468792
  REMARK    GeneRIF: Uroplakin 1a Interacts with Regucalcin and Proteasome
            Subunit Beta 1.
REFERENCE   3  (bases 1 to 2168)
  AUTHORS   Yamaguchi,M., Hashimoto,K., Jijiwa,M. and Murata,T.
  TITLE     The inflammatory macrophages repress the growth of bone metastatic
            human prostate cancer cells via TNF-alpha and IL-6 signaling:
            Involvement of cell signaling regulator regucalcin
  JOURNAL   Cell Signal 107, 110663 (2023)
   PUBMED   37001596
  REMARK    GeneRIF: The inflammatory macrophages repress the growth of bone
            metastatic human prostate cancer cells via TNF-alpha and IL-6
            signaling: Involvement of cell signaling regulator regucalcin.
REFERENCE   4  (bases 1 to 2168)
  AUTHORS   Danish,M. and Ahmad,R.
  TITLE     Functional pleiotropy of calcium binding protein Regucalcin in
            signaling and diseases
  JOURNAL   Cell Signal 102, 110533 (2023)
   PUBMED   36442591
  REMARK    GeneRIF: Functional pleiotropy of calcium binding protein
            Regucalcin in signaling and diseases.
            Review article
REFERENCE   5  (bases 1 to 2168)
  AUTHORS   Peter,C.D., Shashidara,R., Jain,V., Haragannavar,V.C., Samuel,P.
            and Nayak,S.R.
  TITLE     Senescence in oral lichen planus as assessed by the
            immunohistochemical evaluation of senescence marker protein-30
            (Regucalcin)
  JOURNAL   Indian J Pathol Microbiol 66 (1), 9-13 (2023)
   PUBMED   36656203
  REMARK    GeneRIF: Senescence in oral lichen planus as assessed by the
            immunohistochemical evaluation of senescence marker protein-30
            (Regucalcin).
REFERENCE   6  (bases 1 to 2168)
  AUTHORS   Misawa,H. and Yamaguchi,M.
  TITLE     Transcript heterogeneity of the human gene for Ca2+-binding protein
            regucalcin
  JOURNAL   Int J Mol Med 5 (3), 283-287 (2000)
   PUBMED   10677570
REFERENCE   7  (bases 1 to 2168)
  AUTHORS   Fujita,T., Shirasawa,T. and Maruyama,N.
  TITLE     Expression and structure of senescence marker protein-30 (SMP30)
            and its biological significance
  JOURNAL   Mech Ageing Dev 107 (3), 271-280 (1999)
   PUBMED   10360682
  REMARK    Review article
REFERENCE   8  (bases 1 to 2168)
  AUTHORS   Fujita,T.
  TITLE     Senescence marker protein-30 (SMP30): structure and biological
            function
  JOURNAL   Biochem Biophys Res Commun 254 (1), 1-4 (1999)
   PUBMED   9920722
REFERENCE   9  (bases 1 to 2168)
  AUTHORS   Fujita,T., Inoue,H., Kitamura,T., Sato,N., Shimosawa,T. and
            Maruyama,N.
  TITLE     Senescence marker protein-30 (SMP30) rescues cell death by
            enhancing plasma membrane Ca(2+)-pumping activity in Hep G2 cells
  JOURNAL   Biochem Biophys Res Commun 250 (2), 374-380 (1998)
   PUBMED   9753637
REFERENCE   10 (bases 1 to 2168)
  AUTHORS   Fujita,T., Mandel,J.L., Shirasawa,T., Hino,O., Shirai,T. and
            Maruyama,N.
  TITLE     Isolation of cDNA clone encoding human homologue of senescence
            marker protein-30 (SMP30) and its location on the X chromosome
  JOURNAL   Biochim Biophys Acta 1263 (3), 249-252 (1995)
   PUBMED   7548213
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC050371.1, D31815.1 and
            HY260443.1.
            
            On Nov 22, 2018 this sequence version replaced NM_152869.3.
            
            Summary: The protein encoded by this gene is a highly conserved,
            calcium-binding protein, that is preferentially expressed in the
            liver and kidney. It may have an important role in calcium
            homeostasis. Studies in rat indicate that this protein may also
            play a role in aging, as it shows age-associated down-regulation.
            This gene is part of a gene cluster on chromosome Xp11.3-Xp11.23.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Sep 2013].
            
            Transcript Variant: This variant (2) represents the longest
            transcript. Variants 1 and 2 encode the same isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.100031.1,
                                           SRR1803614.192586.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968832
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000397180.6/ ENSP00000380365.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-809               BC050371.1         27-835
            810-2165            D31815.1           1-1356
            2166-2168           HY260443.1         2-4                 c
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xp11.3"
     gene            1..2168
                     /gene="RGN"
                     /gene_synonym="GNL; HEL-S-41; RC; SMP30"
                     /note="regucalcin"
                     /db_xref="GeneID:9104"
                     /db_xref="HGNC:HGNC:9989"
                     /db_xref="MIM:300212"
     exon            1..267
                     /gene="RGN"
                     /gene_synonym="GNL; HEL-S-41; RC; SMP30"
                     /inference="alignment:Splign:2.1.0"
     exon            268..887
                     /gene="RGN"
                     /gene_synonym="GNL; HEL-S-41; RC; SMP30"
                     /inference="alignment:Splign:2.1.0"
     exon            888..1065
                     /gene="RGN"
                     /gene_synonym="GNL; HEL-S-41; RC; SMP30"
                     /inference="alignment:Splign:2.1.0"
     CDS             903..1802
                     /gene="RGN"
                     /gene_synonym="GNL; HEL-S-41; RC; SMP30"
                     /EC_number="3.1.1.17"
                     /note="isoform 1 is encoded by transcript variant 2;
                     senescence marker protein 30; gluconolactonase; epididymis
                     secretory protein Li 41"
                     /codon_start=1
                     /product="regucalcin isoform 1"
                     /protein_id="NP_690608.1"
                     /db_xref="CCDS:CCDS14272.1"
                     /db_xref="GeneID:9104"
                     /db_xref="HGNC:HGNC:9989"
                     /db_xref="MIM:300212"
                     /translation="MSSIKIECVLPENCRCGESPVWEEVSNSLLFVDIPAKKVCRWDS
                     FTKQVQRVTMDAPVSSVALRQSGGYVATIGTKFCALNWKEQSAVVLATVDNDKKNNRF
                     NDGKVDPAGRYFAGTMAEETAPAVLERHQGALYSLFPDHHVKKYFDQVDISNGLDWSL
                     DHKIFYYIDSLSYSVDAFDYDLQTGQISNRRSVYKLEKEEQIPDGMCIDAEGKLWVAC
                     YNGGRVIRLDPVTGKRLQTVKLPVDKTTSCCFGGKNYSEMYVTCARDGMDPEGLLRQP
                     EAGGIFKITGLGVKGIAPYSYAG"
     exon            1066..1248
                     /gene="RGN"
                     /gene_synonym="GNL; HEL-S-41; RC; SMP30"
                     /inference="alignment:Splign:2.1.0"
     exon            1249..1464
                     /gene="RGN"
                     /gene_synonym="GNL; HEL-S-41; RC; SMP30"
                     /inference="alignment:Splign:2.1.0"
     exon            1465..1596
                     /gene="RGN"
                     /gene_synonym="GNL; HEL-S-41; RC; SMP30"
                     /inference="alignment:Splign:2.1.0"
     exon            1597..1751
                     /gene="RGN"
                     /gene_synonym="GNL; HEL-S-41; RC; SMP30"
                     /inference="alignment:Splign:2.1.0"
     exon            1752..2168
                     /gene="RGN"
                     /gene_synonym="GNL; HEL-S-41; RC; SMP30"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2126..2131
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RGN"
                     /gene_synonym="GNL; HEL-S-41; RC; SMP30"
                     /note="hexamer: ATTAAA"
     regulatory      2144..2149
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RGN"
                     /gene_synonym="GNL; HEL-S-41; RC; SMP30"
                     /note="hexamer: AATAAA"
     polyA_site      2149
                     /gene="RGN"
                     /gene_synonym="GNL; HEL-S-41; RC; SMP30"
                     /note="major polyA site"
     polyA_site      2168
                     /gene="RGN"
                     /gene_synonym="GNL; HEL-S-41; RC; SMP30"
                     /note="major polyA site"
ORIGIN      
        1 agacccgctc atcccgatct cccagaaggc gactgacagc tgactgccag aaggagatcg
       61 cgccaggaga ctgactgctc tgtgcccacc cggggacccg ggcccgttca gccgggctgg
      121 ctggtgcgcc ctctgcaaag cctgcgccag ggaggaggca ggctcaacct tcagattccc
      181 agggcctctc tgtcgctgtc gccgtcgccg tcgcccgagg tcccagcggc tctaccagat
      241 tgttgtggag gcctctcacc cgcacagata atttttctct gtcttctccc agttcgctgg
      301 tcatggtctc tggcctaaag tcccagcata gactgttcct ctcctgtgga gtctaacact
      361 ctttgccaaa ctggcacggt gtcttcctgg gaagcaagaa atcactgagg gcctgtcagt
      421 tcctgccagg gtggttcgat gctgtccttt tctccactcc ttccatggtc tgcgttggaa
      481 aatgtgtgtt agttcgaatt cagcgagttc tcattggggt gaacatagtc ctaaaaatgc
      541 agttcataaa aatcaaccac acagagaaga aaaataaata aatcagtaaa gcagctcaga
      601 aaaatgcagg tgtggcgggc cctggcctct gcaccctcat agaggggctc aacagcatca
      661 acagaaggtg ggggagcaga aggtgccctg cagacaccca gaggggccac caatgagcaa
      721 actgccagct ccctgacccg ccctggggcc cagggtattg acataaagga cggccttgca
      781 ctatttcaac agccagttct ctgagggtca caaacaccaa ggagtggagg tcagagtgtc
      841 acttttttgt tttctttttg aaagatcatt cgagaaacac gtcactgatc tcccctgcga
      901 ccatgtcttc cattaagatt gagtgtgttt tgccagagaa ctgccggtgt ggtgagtctc
      961 cagtatggga ggaagtgtcc aactctctgc tctttgtaga cattcctgca aaaaaggttt
     1021 gccggtggga ttcattcacc aagcaagtac agcgagtgac catggatgcc ccagtcagct
     1081 ccgtggctct tcgccagtcg ggaggctatg ttgccaccat tggaacaaag ttctgtgctt
     1141 tgaactggaa agaacaatca gcagttgtct tggccacggt ggataacgac aagaaaaaca
     1201 atcgcttcaa tgatgggaag gtggatcccg ccgggaggta ctttgctggc accatggctg
     1261 aggaaacagc tccagcagtt cttgagcggc accagggggc cctgtactcc ctctttcctg
     1321 atcaccacgt gaaaaagtac tttgaccagg tggacatttc caatggtttg gattggtcgc
     1381 tagaccacaa aatcttctat tacattgaca gcctgtccta ctccgtggat gcctttgact
     1441 atgacctgca gacaggacag atctccaacc gcagaagtgt ttacaagcta gaaaaggaag
     1501 aacaaatccc agatggaatg tgtattgatg ctgaggggaa gctctgggtg gcctgttaca
     1561 atggaggaag agtgattcgt ttagatcctg tgacagggaa aagacttcaa actgtgaagt
     1621 tgcctgttga taaaacaact tcatgctgct ttggagggaa gaattactct gaaatgtatg
     1681 tgacctgcgc ccgggatggg atggaccccg agggtctttt gaggcaacct gaagctggtg
     1741 gaattttcaa gataactggt ctgggggtca aaggaattgc tccctactcc tatgcgggat
     1801 gaggacaggt cttctttcct gccagaggga gctctgaaga caactagaga attctgggcc
     1861 tgaaatttca atctagttag aaagaaaaat gaggcaatga ttttattaac agcgttaagt
     1921 tttaatttac aacttttaaa aggcagagca tttttaacaa ggggtgacag gtggttttga
     1981 taacacactt ataaggcttt ctgtaaaagg tactatagaa gggcgaagaa tcgttcaact
     2041 gtcaatcagc ctcttgattc tttgtaaatt gccagggtgg gtgggtacat atctcttctt
     2101 gattctgcat ttcatactta actatattaa agcttcaagg aacaataaat agtaacctgg
     2161 taatgacc
//
