LOCUS       NP_064596                450 aa            linear   PRI 29-APR-2025
DEFINITION  repulsive guidance molecule A isoform 3 precursor [Homo sapiens].
ACCESSION   NP_064596
VERSION     NP_064596.2
DBSOURCE    REFSEQ: accession NM_020211.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 450)
  AUTHORS   Lun,J., Zhang,Y., Yu,M., Zhai,W., Zhu,L., Liu,H., Guo,J., Zhang,G.,
            Qiu,W. and Fang,J.
  TITLE     Circular RNA circHIPK2 inhibits colon cancer cells through
            miR-373-3p/RGMA axis
  JOURNAL   Cancer Lett 593, 216957 (2024)
   PUBMED   38762192
  REMARK    GeneRIF: Circular RNA circHIPK2 inhibits colon cancer cells through
            miR-373-3p/RGMA axis.
REFERENCE   2  (residues 1 to 450)
  AUTHORS   Liu,D., Zusman,B.E., Shaffer,J.R., Li,Y., Arockiaraj,A.I., Liu,S.,
            Weeks,D.E., Desai,S.M., Kochanek,P.M., Puccio,A.M., Okonkwo,D.O.,
            Conley,Y.P. and Jha,R.M.
  TITLE     Decreased DNA Methylation of RGMA is Associated with Intracranial
            Hypertension After Severe Traumatic Brain Injury: An Exploratory
            Epigenome-Wide Association Study
  JOURNAL   Neurocrit Care 37 (1), 26-37 (2022)
   PUBMED   35028889
  REMARK    GeneRIF: Decreased DNA Methylation of RGMA is Associated with
            Intracranial Hypertension After Severe Traumatic Brain Injury: An
            Exploratory Epigenome-Wide Association Study.
REFERENCE   3  (residues 1 to 450)
  AUTHORS   Kousathanas,A., Pairo-Castineira,E., Rawlik,K., Stuckey,A.,
            Odhams,C.A., Walker,S., Russell,C.D., Malinauskas,T., Wu,Y.,
            Millar,J., Shen,X., Elliott,K.S., Griffiths,F., Oosthuyzen,W.,
            Morrice,K., Keating,S., Wang,B., Rhodes,D., Klaric,L., Zechner,M.,
            Parkinson,N., Siddiq,A., Goddard,P., Donovan,S., Maslove,D.,
            Nichol,A., Semple,M.G., Zainy,T., Maleady-Crowe,F., Todd,L.,
            Salehi,S., Knight,J., Elgar,G., Chan,G., Arumugam,P., Patch,C.,
            Rendon,A., Bentley,D., Kingsley,C., Kosmicki,J.A., Horowitz,J.E.,
            Baras,A., Abecasis,G.R., Ferreira,M.A.R., Justice,A., Mirshahi,T.,
            Oetjens,M., Rader,D.J., Ritchie,M.D., Verma,A., Fowler,T.A.,
            Shankar-Hari,M., Summers,C., Hinds,C., Horby,P., Ling,L.,
            McAuley,D., Montgomery,H., Openshaw,P.J.M., Elliott,P., Walsh,T.,
            Tenesa,A., Fawkes,A., Murphy,L., Rowan,K., Ponting,C.P., Vitart,V.,
            Wilson,J.F., Yang,J., Bretherick,A.D., Scott,R.H., Hendry,S.C.,
            Moutsianas,L., Law,A., Caulfield,M.J. and Baillie,J.K.
  CONSRTM   GenOMICC investigators; 23andMe investigators; COVID-19 Human
            Genetics Initiative
  TITLE     Whole-genome sequencing reveals host factors underlying critical
            COVID-19
  JOURNAL   Nature 607 (7917), 97-103 (2022)
   PUBMED   35255492
REFERENCE   4  (residues 1 to 450)
  AUTHORS   Tang,J., Zeng,X., Li,H., Ju,L., Feng,J. and Yang,J.
  TITLE     Repulsive Guidance Molecule-a and Central Nervous System Diseases
  JOURNAL   Biomed Res Int 2021, 5532116 (2021)
   PUBMED   33997000
  REMARK    GeneRIF: Repulsive Guidance Molecule-a and Central Nervous System
            Diseases.
            Review article
            Publication Status: Online-Only
REFERENCE   5  (residues 1 to 450)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (residues 1 to 450)
  AUTHORS   Xia,Y., Yu,P.B., Sidis,Y., Beppu,H., Bloch,K.D., Schneyer,A.L. and
            Lin,H.Y.
  TITLE     Repulsive guidance molecule RGMa alters utilization of bone
            morphogenetic protein (BMP) type II receptors by BMP2 and BMP4
  JOURNAL   J Biol Chem 282 (25), 18129-18140 (2007)
   PUBMED   17472960
  REMARK    GeneRIF: RGMa facilitates the use of ActRIIA by endogenous BMP2 and
            BMP4 ligands that otherwise prefer signaling via BMPRII and
            increased utilization of ActRIIA leads to generation of an enhanced
            BMP signal
REFERENCE   7  (residues 1 to 450)
  AUTHORS   Schwab,J.M., Monnier,P.P., Schluesener,H.J., Conrad,S.,
            Beschorner,R., Chen,L., Meyermann,R. and Mueller,B.K.
  TITLE     Central nervous system injury-induced repulsive guidance molecule
            expression in the adult human brain
  JOURNAL   Arch Neurol 62 (10), 1561-1568 (2005)
   PUBMED   16216939
  REMARK    GeneRIF: Following central nervous system injury, RGM, a novel,
            potent axonal growth inhibitor, is present in axonal growth
            impediments: the mature myelin, choroid plexus, and components of
            the developing scar.
REFERENCE   8  (residues 1 to 450)
  AUTHORS   Babitt,J.L., Zhang,Y., Samad,T.A., Xia,Y., Tang,J., Campagna,J.A.,
            Schneyer,A.L., Woolf,C.J. and Lin,H.Y.
  TITLE     Repulsive guidance molecule (RGMa), a DRAGON homologue, is a bone
            morphogenetic protein co-receptor
  JOURNAL   J Biol Chem 280 (33), 29820-29827 (2005)
   PUBMED   15975920
REFERENCE   9  (residues 1 to 450)
  AUTHORS   Rajagopalan,S., Deitinghoff,L., Davis,D., Conrad,S., Skutella,T.,
            Chedotal,A., Mueller,B.K. and Strittmatter,S.M.
  TITLE     Neogenin mediates the action of repulsive guidance molecule
  JOURNAL   Nat Cell Biol 6 (8), 756-762 (2004)
   PUBMED   15258590
REFERENCE   10 (residues 1 to 450)
  AUTHORS   Brinks,H., Conrad,S., Vogt,J., Oldekamp,J., Sierra,A.,
            Deitinghoff,L., Bechmann,I., Alvarez-Bolado,G., Heimrich,B.,
            Monnier,P.P., Mueller,B.K. and Skutella,T.
  TITLE     The repulsive guidance molecule RGMa is involved in the formation
            of afferent connections in the dentate gyrus
  JOURNAL   J Neurosci 24 (15), 3862-3869 (2004)
   PUBMED   15084667
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA805034.1, AL136826.1,
            AC087641.5 and AC013394.12.
            
            On Oct 23, 2009 this sequence version replaced NP_064596.1.
            
            Summary: This gene encodes a member of the repulsive guidance
            molecule family. The encoded protein is a
            glycosylphosphatidylinositol-anchored glycoprotein that functions
            as an axon guidance protein in the developing and adult central
            nervous system. This protein may also function as a tumor
            suppressor in some cancers. Alternate splicing results in multiple
            transcript variants. [provided by RefSeq, Oct 2009].
            
            Transcript Variant: This variant (4) differs in the 5' UTR, lacks a
            portion of the 5' coding region, and initiates translation at an
            alternate start codon, compared to variant 1. The encoded isoform
            (3) has a distinct N-terminus and is shorter than isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK074910.1, AL136826.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            coronavirus related    :: locus in the vicinity of
                                      disease-associated variant(s)
            MANE Ensembl match     :: ENST00000329082.12/ ENSP00000330005.7
            RefSeq Select criteria :: based on manual assertion, conservation
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q26.1"
     Protein         1..450
                     /product="repulsive guidance molecule A isoform 3
                     precursor"
                     /note="repulsive guidance molecule A; RGM domain family,
                     member A; repulsive guidance molecule family member a"
                     /calculated_mol_wt=44373
     sig_peptide     1..47
                     /inference="COORDINATES: ab initio prediction:SignalP:6.0"
                     /calculated_mol_wt=5007
     Region          48..221
                     /region_name="RGM_N"
                     /note="Repulsive guidance molecule (RGM) N-terminus;
                     pfam06535"
                     /db_xref="CDD:461940"
     Region          113..140
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q96B86.4)"
     Site            114
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q96B86.4)"
     Site            159
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q96B86.4)"
     Site            168..169
                     /site_type="cleavage"
                     /note="Cleavage, by autolysis. /evidence=ECO:0000250;
                     propagated from UniProtKB/Swiss-Prot (Q96B86.4)"
     mat_peptide     169..424
                     /product="Repulsive guidance molecule A.
                     /id=PRO_0000030386"
                     /note="propagated from UniProtKB/Swiss-Prot (Q96B86.4)"
                     /calculated_mol_wt=28482
     Region          226..406
                     /region_name="RGM_C"
                     /note="Repulsive guidance molecule (RGM) C-terminus;
                     pfam06534"
                     /db_xref="CDD:461939"
     Site            389
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q96B86.4)"
     CDS             1..450
                     /gene="RGMA"
                     /gene_synonym="RGM"
                     /coded_by="NM_020211.3:280..1632"
                     /note="isoform 3 precursor is encoded by transcript
                     variant 4"
                     /db_xref="CCDS:CCDS45357.1"
                     /db_xref="GeneID:56963"
                     /db_xref="HGNC:HGNC:30308"
                     /db_xref="MIM:607362"
ORIGIN      
        1 mqpprerlvv tgragwmgmg rgagrsalgf wptlafllcs fpaatspcki lkcnsefwsa
       61 tsgshapasd dtpefcaalr syalctrrta rtcrgdlayh savhgiedlm sqhncskdgp
      121 tsqprlrtlp pagdsqersd speichyeks fhkhsatpny thcglfgdph lrtftdrfqt
      181 ckvqgawpli dnnylnvqvt ntpvlpgsaa tatskltiif knfqecvdqk vyqaemdelp
      241 aafvdgskng gdkhganslk itekvsgqhv eiqakyigtt ivvrqvgryl tfavrmpeev
      301 vnavedwdsq glylclrgcp lnqqidfqaf htnaegtgar rlaaaspapt apetfpyeta
      361 vakckeklpv edlyyqacvf dllttgdvnf tlaayyaled vkmlhsnkdk lhlyertrdl
      421 pgraaaglpl aprpllgalv pllallpvfc
//
