LOCUS       NM_007370               2104 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens replication factor C subunit 5 (RFC5), transcript
            variant 1, mRNA.
ACCESSION   NM_007370
VERSION     NM_007370.7
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Yao,H., Zhou,X., Zhou,A., Chen,J., Chen,G., Shi,X., Shi,B., Tai,Q.,
            Mi,X., Zhou,G., Wang,S., Sun,J., Yang,X., Yang,Y., Cao,H., Zhou,D.,
            Sun,L., Yao,Y. and He,S.
  TITLE     RFC5, regulated by circ_0038985/miR-3614-5p, functions as an
            oncogene in the progression of colorectal cancer
  JOURNAL   Mol Carcinog 62 (6), 771-785 (2023)
   PUBMED   36988339
  REMARK    GeneRIF: RFC5, regulated by circ_0038985/miR-3614-5p, functions as
            an oncogene in the progression of colorectal cancer.
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Li,W.J., Wu,D.W., Zhou,Y.F., Zhang,C.W. and Liao,X.W.
  TITLE     Prognostic biomarker replication factor C subunit 5 and its
            correlation with immune infiltrates in acute myeloid leukemia
  JOURNAL   Hematology 27 (1), 555-564 (2022)
   PUBMED   35544695
  REMARK    GeneRIF: Prognostic biomarker replication factor C subunit 5 and
            its correlation with immune infiltrates in acute myeloid leukemia.
REFERENCE   3  (bases 1 to 2104)
  AUTHORS   Zhao,X., Sun,Y., Sun,X., Li,J., Shi,X., Liang,Z., Ma,Y. and
            Zhang,X.
  TITLE     AEG-1 Knockdown Sensitizes Glioma Cells to Radiation Through
            Impairing Homologous Recombination Via Targeting RFC5
  JOURNAL   DNA Cell Biol 40 (7), 895-905 (2021)
   PUBMED   34042508
  REMARK    GeneRIF: AEG-1 Knockdown Sensitizes Glioma Cells to Radiation
            Through Impairing Homologous Recombination Via Targeting RFC5.
REFERENCE   4  (bases 1 to 2104)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 2104)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 2104)
  AUTHORS   Wood,R.D. and Shivji,M.K.
  TITLE     Which DNA polymerases are used for DNA-repair in eukaryotes?
  JOURNAL   Carcinogenesis 18 (4), 605-610 (1997)
   PUBMED   9111189
  REMARK    Review article
REFERENCE   7  (bases 1 to 2104)
  AUTHORS   Mossi,R., Jonsson,Z.O., Allen,B.L., Hardin,S.H. and Hubscher,U.
  TITLE     Replication factor C interacts with the C-terminal side of
            proliferating cell nuclear antigen
  JOURNAL   J Biol Chem 272 (3), 1769-1776 (1997)
   PUBMED   8999859
REFERENCE   8  (bases 1 to 2104)
  AUTHORS   Uhlmann,F., Cai,J., Flores-Rozas,H., Dean,F.B., Finkelstein,J.,
            O'Donnell,M. and Hurwitz,J.
  TITLE     In vitro reconstitution of human replication factor C from its five
            subunits
  JOURNAL   Proc Natl Acad Sci U S A 93 (13), 6521-6526 (1996)
   PUBMED   8692848
REFERENCE   9  (bases 1 to 2104)
  AUTHORS   Okumura,K., Nogami,M., Taguchi,H., Dean,F.B., Chen,M., Pan,Z.Q.,
            Hurwitz,J., Shiratori,A., Murakami,Y., Ozawa,K. et al.
  TITLE     Assignment of the 36.5-kDa (RFC5), 37-kDa (RFC4), 38-kDa (RFC3),
            and 40-kDa (RFC2) subunit genes of human replication factor C to
            chromosome bands 12q24.2-q24.3, 3q27, 13q12.3-q13, and 7q11.23
  JOURNAL   Genomics 25 (1), 274-278 (1995)
   PUBMED   7774928
REFERENCE   10 (bases 1 to 2104)
  AUTHORS   O'Donnell,M., Onrust,R., Dean,F.B., Chen,M. and Hurwitz,J.
  TITLE     Homology in accessory proteins of replicative polymerases--E. coli
            to humans
  JOURNAL   Nucleic Acids Res 21 (1), 1-3 (1993)
   PUBMED   8441605
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AU121576.1 and BC001866.1.
            
            On Nov 23, 2018 this sequence version replaced NM_007370.6.
            
            Summary: This gene encodes the smallest subunit of the replication
            factor C complex, which consists of five distinct subunits (140,
            40, 38, 37, and 36 kDa) and is required for DNA replication. This
            subunit interacts with the C-terminal region of proliferating cell
            nuclear antigen and is required to open and load proliferating cell
            nuclear antigen onto DNA during S phase. It is a member of the AAA+
            (ATPases associated with various cellular activities) ATPase family
            and forms a core complex with the 38 and 40 kDa subunits that
            possesses DNA-dependent ATPase activity. A related pseudogene has
            been identified on chromosome 9. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, Nov 2016].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC001866.1, SRR1803611.126310.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000454402.7/ ENSP00000408295.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-24                AU121576.1         3-26
            25-2104             BC001866.1         1-2080
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q24.23"
     gene            1..2104
                     /gene="RFC5"
                     /gene_synonym="RFC36"
                     /note="replication factor C subunit 5"
                     /db_xref="GeneID:5985"
                     /db_xref="HGNC:HGNC:9973"
                     /db_xref="MIM:600407"
     exon            1..190
                     /gene="RFC5"
                     /gene_synonym="RFC36"
                     /inference="alignment:Splign:2.1.0"
     CDS             126..1148
                     /gene="RFC5"
                     /gene_synonym="RFC36"
                     /note="isoform 1 is encoded by transcript variant 1; RFC,
                     36.5 kD subunit; A1 36 kDa subunit; replication factor C,
                     36-kDa subunit; replication factor C (activator 1) 5,
                     36.5kDa; RF-C 36 kDa subunit"
                     /codon_start=1
                     /product="replication factor C subunit 5 isoform 1"
                     /protein_id="NP_031396.1"
                     /db_xref="CCDS:CCDS9185.1"
                     /db_xref="GeneID:5985"
                     /db_xref="HGNC:HGNC:9973"
                     /db_xref="MIM:600407"
                     /translation="METSALKQQEQPAATKIRNLPWVEKYRPQTLNDLISHQDILSTI
                     QKFINEDRLPHLLLYGPPGTGKTSTILACAKQLYKDKEFGSMVLELNASDDRGIDIIR
                     GPILSFASTRTIFKKGFKLVILDEADAMTQDAQNALRRVIEKFTENTRFCLICNYLSK
                     IIPALQSRCTRFRFGPLTPELMVPRLEHVVEEEKVDISEDGMKALVTLSSGDMRRALN
                     ILQSTNMAFGKVTEETVYTCTGHPLKSDIANILDWMLNQDFTTAYRNITELKTLKGLA
                     LHDILTEIHLFVHRVDFPSSVRIHLLTKMADIEYRLSVGTNEKIQLSSLIAAFQVTRD
                     LIVAEA"
     exon            191..255
                     /gene="RFC5"
                     /gene_synonym="RFC36"
                     /inference="alignment:Splign:2.1.0"
     exon            256..392
                     /gene="RFC5"
                     /gene_synonym="RFC36"
                     /inference="alignment:Splign:2.1.0"
     exon            393..472
                     /gene="RFC5"
                     /gene_synonym="RFC36"
                     /inference="alignment:Splign:2.1.0"
     exon            473..546
                     /gene="RFC5"
                     /gene_synonym="RFC36"
                     /inference="alignment:Splign:2.1.0"
     exon            547..706
                     /gene="RFC5"
                     /gene_synonym="RFC36"
                     /inference="alignment:Splign:2.1.0"
     exon            707..788
                     /gene="RFC5"
                     /gene_synonym="RFC36"
                     /inference="alignment:Splign:2.1.0"
     exon            789..918
                     /gene="RFC5"
                     /gene_synonym="RFC36"
                     /inference="alignment:Splign:2.1.0"
     exon            919..996
                     /gene="RFC5"
                     /gene_synonym="RFC36"
                     /inference="alignment:Splign:2.1.0"
     exon            997..1051
                     /gene="RFC5"
                     /gene_synonym="RFC36"
                     /inference="alignment:Splign:2.1.0"
     exon            1052..2104
                     /gene="RFC5"
                     /gene_synonym="RFC36"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1959..1964
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RFC5"
                     /gene_synonym="RFC36"
                     /note="hexamer: AACAAA"
     polyA_site      1988
                     /gene="RFC5"
                     /gene_synonym="RFC36"
     regulatory      2076..2081
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RFC5"
                     /gene_synonym="RFC36"
                     /note="hexamer: AATAAA"
     polyA_site      2104
                     /gene="RFC5"
                     /gene_synonym="RFC36"
                     /note="major polyA site"
ORIGIN      
        1 gagagttgct tttgcgcgcg aactgtaagt gccagggtct cagggtcagg tcgcggctgg
       61 tcactgtgca ggcgcttggg tgacgcgacg atctcagcgg atctggtcac cttcgtctcc
      121 ccgccatgga gacctcagca ctcaagcagc aggagcagcc cgcggcgacc aagatcagga
      181 acctgccctg ggttgaaaaa taccggccac agaccctgaa tgatctcatt tctcatcagg
      241 acattctgag taccattcag aagtttatca atgaagaccg actgccacac ttgcttctct
      301 acggtccccc agggacaggc aagacatcta ccatcctagc ctgtgcgaaa cagctatata
      361 aagacaaaga atttggctcc atggtcttgg agctgaatgc ttcagatgac cgaggaatag
      421 acatcattcg aggaccgatc ctgagctttg ctagcacaag gacaatattt aagaaaggct
      481 ttaagctagt gatcttggat gaagcagacg ccatgactca ggacgcccag aatgccttga
      541 gaagagtaat tgagaaattc acagaaaata ccagattctg cctcatctgt aactatctgt
      601 caaagatcat ccctgccttg cagtcccgct gcacgaggtt tcggttcggt cccctgactc
      661 ctgaactcat ggttccccgc ctggaacatg tcgtggaaga agagaaagtt gatataagtg
      721 aagatggaat gaaagcacta gtcactcttt ccagtggaga catgcgtagg gctctgaaca
      781 ttttgcagag caccaatatg gcctttggga aggtgacaga ggagactgtc tacacctgca
      841 ccgggcaccc gctcaagtca gacattgcca acatcctgga ctggatgttg aatcaagatt
      901 tcaccacagc ctacagaaat attacagagt tgaaaactct gaaggggttg gcactgcatg
      961 atatcctgac agagatacac ttgtttgtgc atagagttga ctttccatct tcagttcgaa
     1021 tacatttatt gaccaaaatg gcagacattg agtacaggct ttctgttggc accaacgaga
     1081 agatccagct gagctccctc attgctgcat ttcaagtcac cagagacctg attgttgcag
     1141 aggcctagat gctctgaggg ccattcacaa ttctcagggc tcagcagtga tgggagaaca
     1201 gaggacagtt ccaggataaa ctgctgcctg gggctgtggg atgaatcagt caccccgaat
     1261 cttggaaaaa cccccttcca ggagaggatg ggcaggcatt taaaaagtac catttttgtg
     1321 gttgtttgga gcagggatgt acaaaataat tttaatgtat taactcatac tgcctgtctt
     1381 ttatagggga aaaaaataac cttttttatt ttaaagttat aaggttttta ccttttagtt
     1441 gcttggatga cagggaatta gcctacccca ttttggtctg gaacagaaga ctttcaaatt
     1501 taatatggtc caagtgtctt cctactcaag gtaaacatta tctccaaaat tacatttatg
     1561 attctaatat ttggcattgt gtctgtatct aatttaaaca gatgttaatg gacgtctggc
     1621 cgaaactatt atacttttat aagatgagct gaatcctctt actttaaaaa ctggtctttt
     1681 tatttaccct ctgtggtaga agagtcacca gcaggttcca aattgatgtg tatgatagga
     1741 aaaaaacctt aattttaaat ataatataga gccttaaact atgccactgg gtggcagagg
     1801 ctgtataaaa cgcacttgtt ttcatgcagg agcggggcaa gtaaggttga gcctgactgt
     1861 aaacctagaa ctgcggaagg actgggactt ttgtacaaat ttcacattta ttttacagca
     1921 atcatgctgc atttgtgtta gtgttcttta taataaaaaa caaagcagcc tcctggctgg
     1981 ctgtgttaca agtgactctt ccttagacag ctagaaagat gctgactttg tcaaacttgc
     2041 atttatatga aaaccttctg tctctaatat cacagaataa actttcttta gatgctgttc
     2101 ttta
//
