LOCUS       NM_181471               1685 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens replication factor C subunit 2 (RFC2), transcript
            variant 1, mRNA.
ACCESSION   NM_181471
VERSION     NM_181471.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Lou,F. and Zhang,M.
  TITLE     RFC2 promotes aerobic glycolysis and progression of colorectal
            cancer
  JOURNAL   BMC Gastroenterol 23 (1), 353 (2023)
   PUBMED   37821801
  REMARK    GeneRIF: RFC2 promotes aerobic glycolysis and progression of
            colorectal cancer.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Li,H., Chai,L., Ding,Z. and He,H.
  TITLE     CircCOL1A2 Sponges MiR-1286 to Promote Cell Invasion and Migration
            of Gastric Cancer by Elevating Expression of USP10 to Downregulate
            RFC2 Ubiquitination Level
  JOURNAL   J Microbiol Biotechnol 32 (7), 938-948 (2022)
   PUBMED   35791074
  REMARK    GeneRIF: CircCOL1A2 Sponges MiR-1286 to Promote Cell Invasion and
            Migration of Gastric Cancer by Elevating Expression of USP10 to
            Downregulate RFC2 Ubiquitination Level.
REFERENCE   3  (bases 1 to 1685)
  AUTHORS   Zhao,X., Wang,Y., Li,J., Qu,F., Fu,X., Liu,S., Wang,X., Xie,Y. and
            Zhang,X.
  TITLE     RFC2: a prognosis biomarker correlated with the immune signature in
            diffuse lower-grade gliomas
  JOURNAL   Sci Rep 12 (1), 3122 (2022)
   PUBMED   35210438
  REMARK    GeneRIF: RFC2: a prognosis biomarker correlated with the immune
            signature in diffuse lower-grade gliomas.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1685)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 1685)
  AUTHORS   Ji,Z., Li,J. and Wang,J.
  TITLE     Up-regulated RFC2 predicts unfavorable progression in
            hepatocellular carcinoma
  JOURNAL   Hereditas 158 (1), 17 (2021)
   PUBMED   34022962
  REMARK    GeneRIF: Up-regulated RFC2 predicts unfavorable progression in
            hepatocellular carcinoma.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1685)
  AUTHORS   Uhlmann,F., Cai,J., Flores-Rozas,H., Dean,F.B., Finkelstein,J.,
            O'Donnell,M. and Hurwitz,J.
  TITLE     In vitro reconstitution of human replication factor C from its five
            subunits
  JOURNAL   Proc Natl Acad Sci U S A 93 (13), 6521-6526 (1996)
   PUBMED   8692848
REFERENCE   7  (bases 1 to 1685)
  AUTHORS   Peoples,R., Perez-Jurado,L., Wang,Y.K., Kaplan,P. and Francke,U.
  TITLE     The gene for replication factor C subunit 2 (RFC2) is within the
            7q11.23 Williams syndrome deletion
  JOURNAL   Am J Hum Genet 58 (6), 1370-1373 (1996)
   PUBMED   8651315
REFERENCE   8  (bases 1 to 1685)
  AUTHORS   Okumura,K., Nogami,M., Taguchi,H., Dean,F.B., Chen,M., Pan,Z.Q.,
            Hurwitz,J., Shiratori,A., Murakami,Y., Ozawa,K. et al.
  TITLE     Assignment of the 36.5-kDa (RFC5), 37-kDa (RFC4), 38-kDa (RFC3),
            and 40-kDa (RFC2) subunit genes of human replication factor C to
            chromosome bands 12q24.2-q24.3, 3q27, 13q12.3-q13, and 7q11.23
  JOURNAL   Genomics 25 (1), 274-278 (1995)
   PUBMED   7774928
REFERENCE   9  (bases 1 to 1685)
  AUTHORS   Pan,Z.Q., Chen,M. and Hurwitz,J.
  TITLE     The subunits of activator 1 (replication factor C) carry out
            multiple functions essential for proliferating-cell nuclear
            antigen-dependent DNA synthesis
  JOURNAL   Proc Natl Acad Sci U S A 90 (1), 6-10 (1993)
   PUBMED   8093561
REFERENCE   10 (bases 1 to 1685)
  AUTHORS   Chen,M., Pan,Z.Q. and Hurwitz,J.
  TITLE     Sequence and expression in Escherichia coli of the 40-kDa subunit
            of activator 1 (replication factor C) of HeLa cells
  JOURNAL   Proc Natl Acad Sci U S A 89 (7), 2516-2520 (1992)
   PUBMED   1313560
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from HY046046.1, HY056452.1,
            AB451243.1, BC002813.1 and AW450467.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_181471.2.
            
            Summary: This gene encodes a member of the activator 1 small
            subunits family. The elongation of primed DNA templates by DNA
            polymerase delta and epsilon requires the action of the accessory
            proteins, proliferating cell nuclear antigen (PCNA) and replication
            factor C (RFC). Replication factor C, also called activator 1, is a
            protein complex consisting of five distinct subunits. This gene
            encodes the 40 kD subunit, which has been shown to be responsible
            for binding ATP and may help promote cell survival. Disruption of
            this gene is associated with Williams syndrome. Alternatively
            spliced transcript variants encoding distinct isoforms have been
            described. A pseudogene of this gene has been defined on chromosome
            2. [provided by RefSeq, Jul 2013].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.357563.1,
                                           SRR3476690.959359.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2162568 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000055077.8/ ENSP00000055077.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-9                 HY046046.1         61-69
            10-16               HY056452.1         63-69
            17-1080             AB451243.1         1-1064
            1081-1523           BC002813.1         968-1410
            1524-1685           AW450467.1         16-177              c
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q11.23"
     gene            1..1685
                     /gene="RFC2"
                     /gene_synonym="RFC40"
                     /note="replication factor C subunit 2"
                     /db_xref="GeneID:5982"
                     /db_xref="HGNC:HGNC:9970"
                     /db_xref="MIM:600404"
     exon            1..129
                     /gene="RFC2"
                     /gene_synonym="RFC40"
                     /inference="alignment:Splign:2.1.0"
     CDS             17..1081
                     /gene="RFC2"
                     /gene_synonym="RFC40"
                     /note="isoform 1 is encoded by transcript variant 1; A1 40
                     kDa subunit; RF-C 40 kDa subunit; activator 1 subunit 2;
                     replication factor C (activator 1) 2, 40kDa"
                     /codon_start=1
                     /product="replication factor C subunit 2 isoform 1"
                     /protein_id="NP_852136.1"
                     /db_xref="CCDS:CCDS5568.1"
                     /db_xref="GeneID:5982"
                     /db_xref="HGNC:HGNC:9970"
                     /db_xref="MIM:600404"
                     /translation="MEVEAVCGGAGEVEAQDSDPAPAFSKAPGSAGHYELPWVEKYRP
                     VKLNEIVGNEDTVSRLEVFAREGNVPNIIIAGPPGTGKTTSILCLARALLGPALKDAM
                     LELNASNDRGIDVVRNKIKMFAQQKVTLPKGRHKIIILDEADSMTDGAQQALRRTMEI
                     YSKTTRFALACNASDKIIEPIQSRCAVLRYTKLTDAQILTRLMNVIEKERVPYTDDGL
                     EAIIFTAQGDMRQALNNLQSTFSGFGFINSENVFKVCDEPHPLLVKEMIQHCVNANID
                     EAYKILAHLWHLGYSPEDIIGNIFRVCKTFQMAEYLKLEFIKEIGYTHMKIAEGVNSL
                     LQMAGLLARLCQKTMAPVAS"
     exon            130..199
                     /gene="RFC2"
                     /gene_synonym="RFC40"
                     /inference="alignment:Splign:2.1.0"
     exon            200..241
                     /gene="RFC2"
                     /gene_synonym="RFC40"
                     /inference="alignment:Splign:2.1.0"
     exon            242..348
                     /gene="RFC2"
                     /gene_synonym="RFC40"
                     /inference="alignment:Splign:2.1.0"
     exon            349..450
                     /gene="RFC2"
                     /gene_synonym="RFC40"
                     /inference="alignment:Splign:2.1.0"
     exon            451..551
                     /gene="RFC2"
                     /gene_synonym="RFC40"
                     /inference="alignment:Splign:2.1.0"
     exon            552..709
                     /gene="RFC2"
                     /gene_synonym="RFC40"
                     /inference="alignment:Splign:2.1.0"
     exon            710..775
                     /gene="RFC2"
                     /gene_synonym="RFC40"
                     /inference="alignment:Splign:2.1.0"
     exon            776..856
                     /gene="RFC2"
                     /gene_synonym="RFC40"
                     /inference="alignment:Splign:2.1.0"
     exon            857..970
                     /gene="RFC2"
                     /gene_synonym="RFC40"
                     /inference="alignment:Splign:2.1.0"
     exon            971..1685
                     /gene="RFC2"
                     /gene_synonym="RFC40"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1503..1508
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RFC2"
                     /gene_synonym="RFC40"
                     /note="hexamer: AATAAA"
     polyA_site      1523
                     /gene="RFC2"
                     /gene_synonym="RFC40"
                     /note="major polyA site"
     regulatory      1658..1663
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RFC2"
                     /gene_synonym="RFC40"
                     /note="hexamer: AATAAA"
     polyA_site      1685
                     /gene="RFC2"
                     /gene_synonym="RFC40"
ORIGIN      
        1 aagaggaggc gcgagaatgg aggtggaggc cgtctgtggt ggcgcgggcg aggtggaggc
       61 ccaggactct gaccctgccc ctgccttcag caaggccccc ggcagcgccg gccactacga
      121 actgccgtgg gttgaaaaat ataggccagt aaagctgaat gaaattgtcg ggaatgaaga
      181 caccgtgagc aggctagagg tctttgcaag ggaaggaaat gtgcccaaca tcatcattgc
      241 gggccctcca ggaaccggca agaccacaag cattctgtgc ttggcccggg ccctgctggg
      301 cccagcactc aaagatgcca tgttggaact caatgcttca aatgacaggg gcattgacgt
      361 tgtgaggaat aaaattaaaa tgtttgctca acaaaaagtc actcttccca aaggccgaca
      421 taagatcatc attctggatg aagcagacag catgaccgac ggagcccagc aagccttgag
      481 gagaaccatg gaaatctact ctaaaaccac tcgcttcgcc cttgcttgta atgcttcgga
      541 taagatcatc gagcccattc agtcccgctg tgcagtcctc cggtacacaa agctgaccga
      601 cgcccagatc ctcaccaggc tgatgaatgt tatcgagaag gagagggtac cctacactga
      661 tgacggccta gaagccatca tcttcacggc ccagggagac atgaggcagg cgctgaacaa
      721 cctgcagtcc accttctcag gatttggctt cattaacagt gagaacgtgt tcaaggtctg
      781 tgacgagccc cacccactgc tggtaaagga gatgatccag cactgtgtga atgccaacat
      841 tgacgaagcc tacaagattc ttgctcactt gtggcatctg ggctactcac cagaagatat
      901 cattggcaac atctttcgag tgtgtaaaac tttccaaatg gcagaatacc tgaaactgga
      961 gtttatcaag gaaattggat acactcacat gaaaatagcg gaaggagtga actctctttt
     1021 gcagatggca ggcctcctgg caaggctgtg tcagaagaca atggccccgg tggccagtta
     1081 gagcagagac ttcactgact gacttacagg tgccctattc tgaggtacag gagccgcggc
     1141 tttctgatgg gggaaaatgc cgccttaggc tggagccaac atgactgtcc tttaaactcc
     1201 agtggctggc caggcacggt agctcacgcc tgtaatccca acactttggg aggccgaggc
     1261 aggtggatca cctgaggtca gaagttcaag accagcctgg ccaacatggg gaaaccctgt
     1321 ctttactaaa aatataaaaa ttagctgggt gtggtggcgg gcacctgtaa tcccagctac
     1381 tcgggaggct gtggcaggag aatcgcttga acccaggagg tggaggttgc agtgagccaa
     1441 gatcacacca ttgcactcca gcctgggcga cagagtctcc atctggggaa aaaaattaaa
     1501 taaataaact cccgtgactt gcatgtttgc ttctgggacg tctgtgcccc gcaagtgttt
     1561 gagtttggcc tccaccccat tgatgcggtg acggggcgga aaggcgcaga gaagctgagg
     1621 gcggtctctg atctgtgtgt gggttgacat tttagctaat aaagccttgc agtgtttgtt
     1681 ggcaa
//
